LOCUS       MR223909                8073 bp    DNA     circular  SYN 
DEFINITION  Pik3cb (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, catalytic, beta polypeptide (Pik3cb)
ACCESSION   NM_029094
VERSION     NM_029094.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4230..4321 
                     /label="Myc-DDK"  
     misc_feature    4351..4936 
                     /label="PolyA signal"  
     misc_feature    4408..4427 
                     /label="Restriction Enzyme"  
     misc_feature    5212..5884 
                     /label="ColE1"  
     misc_feature    6314..7108 
                     /label="Kan/Neo"  
     misc_feature    7143..7445 
                     /label="SV40 ori"  
     misc_feature    7531..8008 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4212..4218 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4212 
                     /label="Pik3cb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCCTGCT ATGGCAGACA ACCTTGACAT CTGGGCAGTG GACTCACAGA 
     1081 TTGCATCCGA TGGCGCCATA TCCGTCGATT TCCTTCTGCC CACCGGGATT TATATCCAGT 
     1141 TGGAAGTACC TCGGGAAGCT ACCATTTCTT ATATTAAACA GATGTTATGG AAGCAAGTTC 
     1201 ACAACTACCC GATGTTTAAC CTCCTCATGG ACATTGACTC GTATATGTTT GCATGTGTGA 
     1261 ATCAAACTGC TGTATATGAG GAACTGGAAG ACGAAACACG AAGACTTTGT GATGTCAGAC 
     1321 CTTTTCTTCC AGTTCTCAAA CTAGTGACTA GAAGCTGTGA CCCCGCAGAA AAATTGGACT 
     1381 CAAAAATTGG GGTTCTTATA GGAAAAGGTC TTCATGAGTT TGATGCCTTG AAGGATCCCG 
     1441 AAGTGAATGA ATTTAGAAGA AAAATGCGCA AATTCAGTGA GGCCAAGATT CAGTCTCTGG 
     1501 TAGGGTTGTC TTGGATCGAC TGGCTAAAGC ACACGTATCC GCCTGAGCAC GAGCCGTCCG 
     1561 TCCTGGAGAA CTTGGAAGAT AAACTTTATG GAGGAAAGCT GGTTGTGGCT GTGCACTTTG 
     1621 AAAATAGCCA GGATGTATTT AGTTTTCAAG TGTCTCCCAA TTTGAATCCT ATAAAAATAA 
     1681 ATGAATTGGC AATCCAGAAA CGCCTCACTA TTCGTGGAAA GGAAGATGAA GCTAGCCCCT 
     1741 GTGACTATGT GTTACAGGTC AGTGGGAGAG TGGAGTATGT GTTTGGCGAT CATCCACTAA 
     1801 TTCAGTTCCA GTACATCCGG AATTGTGTGA TGAATAGAAC CCTGCCCCAC TTCATCCTTG 
     1861 TGGAATGTTG TAAGATCAAG AAAATGTATG AACAAGAAAT GATTGCCATA GAGGCTGCCA 
     1921 TCAACCGAAA CTCATCCAAC CTTCCTCTCC CTTTACCACC AAAGAAAACG CGAGTTATTT 
     1981 CTCATATCTG GGACAACAAC AACCCTTTCC AAATTACCTT GGTTAAAGGA AATAAGCTTA 
     2041 ATACAGAAGA AACTGTGAAA GTTCATGTCC GAGCTGGGCT TTTTCACGGA ACCGAGCTCC 
     2101 TGTGTAAAAC CGTCGTAAGC TCAGAGATAT CAGGAAAGAA CGACCATATT TGGAATGAAC 
     2161 AACTGGAATT TGATATTAAT ATTTGTGACT TACCAAGAAT GGCTCGATTA TGTTTTGCTG 
     2221 TTTATGCAGT TTTGGATAAA GTAAAAACGA AGAAATCAAC AAAGACTATT AATCCCTCTA 
     2281 AGTATCAGAC CATCAGGAAA GCCGGGAAAG TGCATTATCC TGTCGCATGG GTAAATACCA 
     2341 TGGTTTTTGA CTTCAAAGGA CAGCTGAGGT CTGGAGACGT CATATTGCAT AGCTGGTCTT 
     2401 CGTTTCCTGA TGAGCTGGAA GAAATGCTGA ATCCCATGGG GACTGTGCAG ACGAACCCAT 
     2461 ATGCTGAGAA CGCCACCGCC TTGCACATTA CGTTCCCAGA GAATAAGAAG CAGCCGTGTT 
     2521 ATTATCCCCC CTTCGATAAG ATCATTGAGA AGGCAGCTGA GCTTGCCAGC GGAGACAGTG 
     2581 CTAATGTGTC AAGTCGTGGT GGAAAAAAAT TTCTTGCTGT GCTGAAAGAA ATCTTGGACA 
     2641 GGGACCCCCT GTCTCAGCTG TGTGAGAACG AAATGGACCT TATTTGGACT CTACGGCAAG 
     2701 ACTGCCGAGA AAATTTCCCT CAGTCACTGC CAAAACTACT CTTGTCAATC AAGTGGAATA 
     2761 AACTTGAAGA TGTTGCTCAG CTTCAGGCGC TCCTGCAGAT ATGGCCCAAA CTGCCCCCCA 
     2821 GGGAAGCCCT GGAACTCCTG GATTTCAACT ATCCAGACCA GTATGTCCGG GAATACGCTG 
     2881 TAGGCTGCCT TCGACAGATG AGTGATGAAG AACTCTCTCA GTATCTTTTA CAATTGGTGC 
     2941 AAGTTTTGAA ATATGAGCCT TTTCTCGATT GTGCCCTCTC CAGATTCCTA TTAGAAAGAG 
     3001 CACTTGATAA TCGGAGGATT GGGCAGTTTC TGTTTTGGCA TCTTAGGTCA GAGGTGCACA 
     3061 CTCCTGCTGT GTCCGTACAG TTTGGTGTCA TCCTGGAAGC ATACTGTCGA GGAAGCGTGG 
     3121 GGCACATGAA AGTGCTTTCC AAACAGGTGG AAGCACTCAA TAAGTTAAAA ACTTTAAATA 
     3181 GCTTAATCAA ACTGAATGCG GTGAAGCTGA GCAGAGCTAA GGGAAAGGAG GCCATGCACA 
     3241 CGTGCCTGAA ACAGAGTGCT TACCGGGAGG CGCTCTCTGA CCTGCAGTCG CCGCTGAACC 
     3301 CCTGCGTCAT CCTCTCAGAG CTCTATGTTG AAAAGTGCAA ATACATGGAC TCCAAGATGA 
     3361 AGCCCCTGTG GCTGGTCTAC AGCAGCAGAG CCTTTGGAGA GGACTCGGTT GGAGTGATCT 
     3421 TTAAAAATGG TGACGATTTG CGGCAGGACA TGCTGACGCT GCAGATGTTG CGCCTGATGG 
     3481 ATCTGCTTTG GAAAGAAGCT GGCTTGGACC TGCGGATGCT CCCCTATGGC TGCTTAGCAA 
     3541 CAGGAGATCG CTCTGGCCTC ATTGAGGTTG TGAGCACCTC TGAGACAATC GCTGACATTC 
     3601 AGCTGAACAG TAGTAACGTG GCTGCCACGG CAGCCTTCAA CAAAGACGCA CTCCTGAACT 
     3661 GGCTCAAGGA GTACAACTCT GGGGATGACC TGGACCGAGC GATTGAGGAG TTTACCTTGT 
     3721 CCTGTGCTGG CTACTGTGTA GCCTCTTATG TCCTCGGCAT TGGTGACAGG CACAGTGACA 
     3781 ACATCATGGT GAAGAAAACC GGCCAGCTCT TCCACATAGA TTTTGGGCAT ATTCTTGGAA 
     3841 ATTTCAAATC TAAATTTGGC ATTAAAAGGG AGCGAGTACC TTTTATTCTT ACTTATGACT 
     3901 TCATTCATGT CATTCAACAA GGAAAAACGG GAAACACTGA AAAATTTGGC AGATTCCGCC 
     3961 AGTGCTGTGA AGATGCGTAT CTGATTTTAC GGCGGCATGG GAATCTCTTC ATCACCCTGT 
     4021 TTGCCCTGAT GTTGACTGCA GGGCTGCCTG AGCTCACATC GGTCAAAGAT ATACAGTATC 
     4081 TTAAGGACTC GCTTGCCTTA GGGAAGAGCG AGGAGGAAGC ACTGAAGCAG TTCAAGCAGA 
     4141 AGTTTGACGA GGCCCTCAGG GAAAGCTGGA CTACTAAAGT GAACTGGATG GCTCACACAG 
     4201 TACGGAAAGA CTACAGGTCC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4261 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4321 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4381 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4441 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4501 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4561 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4621 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4681 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4741 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4801 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4861 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4921 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4981 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5041 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5101 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5161 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5221 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5281 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5341 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5401 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5461 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5521 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5581 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5641 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5701 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5761 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5821 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5881 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5941 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6001 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6061 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6121 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6181 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6241 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6301 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6361 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6421 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6481 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6541 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6601 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6661 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6721 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6781 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6841 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6901 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6961 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7021 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7081 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7141 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7201 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7261 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7321 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7381 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7441 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7501 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7561 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7621 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7681 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7741 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7801 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7861 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7921 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7981 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8041 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//