LOCUS       MR223878                8877 bp    DNA     circular  SYN 
DEFINITION  Sos2 (Myc-DDK-tagged) - Mouse son of sevenless homolog 2 (Drosophila) (Sos2)
ACCESSION   NM_001135559
VERSION     NM_001135559.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5034..5125 
                     /label="Myc-DDK"  
     misc_feature    5155..5740 
                     /label="PolyA signal"  
     misc_feature    5212..5231 
                     /label="Restriction Enzyme"  
     misc_feature    6016..6688 
                     /label="ColE1"  
     misc_feature    7118..7912 
                     /label="Kan/Neo"  
     misc_feature    7947..8249 
                     /label="SV40 ori"  
     misc_feature    8335..8812 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5016..5022 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5016 
                     /label="Sos2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCAGGCG CCGCAGCCCT ACGAGTTCTT CAGCGAAGAA AACAGCCCGA 
     1081 AATGGCGGGG ACTATTGGTC CCGGCCCTGC GGAAGGTTCA GGAGCAAGTA CATCCCACCC 
     1141 TCTCAGCTAA TGAAGAGTCT CTCTATTATA TTGAAGAACT GATTTTTCAG CTGCTTAATA 
     1201 AGCTATGCAT GGCTCAACCA AGGACTGTTC AAGATGTTGA GGAACGAGTT CAAAAGACCT 
     1261 TTCCTCATCC TATTGATAAA TGGGCAATTG CTGATGCACA ATCTGCTATA GAGAAACGAA 
     1321 AACGAAGAAA TCCTCTCTTA CTACCTGTGG ACAAAATCCA TCCTTCCTTG AAGGAAGTTT 
     1381 TGGGGTATAA AGTGGACTAC CATGTGTCCC TCTACATTGT GGCTGTATTG GAGTATATCT 
     1441 CAGCAGATAT TTTGAAATTG GCTGGTAATT ATGTTTTTAA TATCCGGCAT TATGAAATAT 
     1501 CTCAGCAAGA CATTAAAGTG TCCATGTGTG CAGATAAGGT TTTGATGGAC ATGTTCGATC 
     1561 AGGATGATGA TATAGGCTTG GTTTCTCTCT GTGAAGATGA GCCTTGTTCT TCTGGTGAGC 
     1621 TAAACTATTA TGACCTCGTC AGGACTGAAA TTGCAGAAGA AAGACAGTAT CTACGGGAGC 
     1681 TGAATATGAT CATTAAAGTG TTCCGGGAAG CCTTTCTCTT GGACAGAAAG TTGTTCAAGC 
     1741 CTTCTGAAAT TGAAAAGATT TTCAGTAACA TTTCAGATAT ACATGAATTG ACTGTGAAAC 
     1801 TTTTAGGTTT AATTGAAGAC ACAGTAGAAA TGACAGATGA AAGTAGTCCT CATCCATTAG 
     1861 CTGGTAGCTG TTTTGAAGAT TTAGCAGAGG AGCAAGCGTT TGATCCCTAT GAAACATTAT 
     1921 CACAGGACAT TCTTGCACCA GAGTTTAATG ACCACTTCAG CAAGTTGATG GCCAGACCTG 
     1981 CAGTCGCTCT ACATTTTCAG TCCATTGCTG ACGGCTTTAA GGAGGCTGTT CGTTATGTCC 
     2041 TTCCACGCCT CATGCTGGTT CCCGTGTATC ACTGTTGGCA TTACTTTGAA TTATTAAAGT 
     2101 TGAAGGCATG CAGTGAAGAG CAGGAGGACA AAGAGTGCTT GAATCAGGCT ATAACTGCCC 
     2161 TCATGAACCT CCAAGGCAGC ATGGACCGCA TTTACAAGCA GCACTCCCCC AGACGCCGGC 
     2221 CTGGGGATCC AGTTTGCCTT TTTTACAATC GTCAATTAAG AAGCAAACAC CTGGCTATCA 
     2281 AAAAAATGAA TGAAATTCAG AAAAACATAG ATGGGTGGGA AGGCAAAGAT ATCGGACAGT 
     2341 GTTGTAATGA GTTCATAATG GAGGGGCCAC TGACCAGAAT TGGTGCTAAA CACGAAAGGC 
     2401 ATATCTTTCT CTTTGATGGC TTAATGATCA GCTGTAAACC CAATCATGGC CAGACCCGGC 
     2461 TTCCAGGATA TAGCAGTGCA GAATACAGAT TAAAGGAGAA GTTTGTCATG AGGAAAATTC 
     2521 AAATCTGTGA TAAGGAAGAC GCCTGTGAGT ACAGACATGC TTTTGAATTA GTGTCCAAAG 
     2581 ATGAAAACAG TGTAATATTT GCTGCCAAAT CAGCTGAAGA GAAAAACAAC TGGATGGCAG 
     2641 CCCTCATTTC CCTGCACTAT CGCAGCACTC TAGACAGAAT GCTGGACTCT GTGCTGCTGA 
     2701 AAGAAGAGAA TGAGCAGCCC CTGAGGCTAC CCAGTCCAGA TATGTATCGC TTTGTGGTAA 
     2761 CAGACTCTGA GGAAAACATT GTGTTTGAAG ACAACTTGCA AAGCAGAAGT GGGATCCCCA 
     2821 TAATTAAAGG AGGCACTGTG GTGAAGTTGA TCGAAAGGCT AACATACCAC ATGTATGCAG 
     2881 ATCCCAATTT TGTTCGTACT TTTCTTACTA CATATCGTTC ATTTTGTAAA CCACAGGAAT 
     2941 TGCTAAACTT GCTGATAGAA CGGTTTGAAA TTCCAGAACC AGAACCTACT GAGGCAGACA 
     3001 AGCTGGCGTT AGAAAAAGGC GAGCAGCCAA TCAGCGCAGA TCTGAAAAGA TTCCGCAAGG 
     3061 AATACGTCCA ACCTGTGCAG CTTAGGGTCT TGAATGTCTT TCGCCACTGG GTTGAGCATC 
     3121 ATTATTATGA CTTTGAAAGA GACTTGGAAC TGCTTGAAAG ACTAGAATCC TTCATTTCAA 
     3181 GTGTAAGAGG GAAAGCCATG AAGAAATGGG TAGAATCCAT TGCTAAAATA ATCAAGAGGA 
     3241 AGAAGCAAGC TCAGGCAAAT GGAATAAGCC ATAATATCAC CTTTGAAAGT TCCCCCCCAC 
     3301 CAGTGGAATG GCACATCAGT AGAACAGGAC AGTTCGAAAC ATTTGACCTT ATGACACTTC 
     3361 ATCCAATAGA GATCGCACGG CAGCTAACAC TTTTGGAATC TGACCTCTAC AGGAAAGTCC 
     3421 AGCCCTCTGA ACTTGTAGGG AGTGTCTGGA CCAAAGAAGA TAAAGAAATA AATTCTCCAA 
     3481 ACTTATTAAA AATGATTCGC CATACAACAA ACCTCACCCT ATGGTTTGAG AAATGCATTG 
     3541 TGGAAGCAGA AAACTTTGAA GAACGGGTGG CAGTGCTCAG CAGAATAGTA GAAATTCTGC 
     3601 AAGTATTTCA AGACTTGAAT AATTTCAATG GCGTGTTGGA GATAGTGAGT GCAGTCAACT 
     3661 CCGTGTCAGT GTACAGGCTA GACCACACGT TTGAGGCACT GCAGGAAAGG AAGCGGAGAA 
     3721 TTTTGGATGA CGCTGTGGAA CTAAGTCAGG ACCACTTTAA AAAGTACCTA GTAAAACTTA 
     3781 AGTCAATCAA TCCGCCTTGT GTGCCTTTTT TTGGAATATA TTTAACAAAT ATTCTGAAGA 
     3841 CTGAAGAAGG GAACAGTGAC TTTCTAAAGA GGAAAGGGAA AGATTTGATC AATTTCAGTA 
     3901 AGAGGAGGAA AGTGGCTGAA ATAACTGGAG AGATCCAGCA GTATCAGAAC CAACCGTACT 
     3961 GCTTACGGAC AGAACCAGAA ATGAGGAGAT TCTTTGAAAA CCTCAACCCC ATGGGAATTT 
     4021 TATCTGAAAA AGAGTTTACA GATTATTTGT TCAACAAATC ATTAGAAATC GAACCCCGAA 
     4081 ACTGCAAACA ACCACCTCGA TTTCCTAGGA AGTCAACCTT TTCCTTAAAA TCTCCTGGAA 
     4141 TAAGGCCCAA TGCTGGCCGC CATGGCTCTA CCTCAGGCAC GCTACGAGGT CACCCAACGC 
     4201 CTCTGGAAAG AGAGCCTTAT AAGATAAGCT TTAGCCGGAT CGCTGAGACA GAGCTAGAAT 
     4261 CAACAGTGTC TGCACCAACC TCCCCCAACA CTCCATCCAC CCCACCAGTG TCTGCTTCTT 
     4321 CAGACCACAG CGTGTTTCTA GATGTGGACC TCAATAGCTC CTGTGGCAGC AACACCATCT 
     4381 TTGCTCCAGT CCTCTTGCCA CACTCAAAGA CTTTCTTCAG CTCATGTGGA AGTTTACACA 
     4441 AACTGAGTGA AGAGCCACTA ATTCCTCCTC CGCTTCCCCC TCGGAAAAAG TTTGATCATG 
     4501 ATGCTCTCAA TTCCAAGGGA GCTGTGAAAT CTGATGATGA CCCTCCTGCT ATTCCACCAA 
     4561 GACAGCCCCC TCCTCCGAAG GTAAAGCCAA GAGTTCCTGT CCTCATGGGT ACATTTGATG 
     4621 GGCCTGTGCC CAGTCCACCT CCACCTCCTC CAAGAGACCC TCTTCCTGAT ACCCCTCCAC 
     4681 CAGTTCCTCT TCGGCCTCCG GAACACTTTA TAAACTGTCC ATTTAATCTT CAGCCGCCTC 
     4741 CACTGGGCCA TCCTCACAGA GACCCAGACT GGCTCAGAGA CGTCAGCACG TGTCCTAACT 
     4801 CACCAAGCAC TCCTCCCACT ACGCCCTCTC CACGGATTCC ACGCAGCTGT CACTTGCTCA 
     4861 GCTCCAGTCA CAGCAGCCTT GCTCATCTTC CAGCTCCTCC TGTCCCACCA AGGCAGAATT 
     4921 CAAGCCCTCT CTTACCAAAG CTGCCACCAA AGACTTACAA ACGGGAGCTT TCCCACCCGC 
     4981 CACTGTATAG ACTGCCTCTG CTGGAAAATG CAGAAACTCC TCAAACGCGT ACGCGGCCGC 
     5041 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5101 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5161 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5221 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5281 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5341 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5401 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5461 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5521 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5581 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5641 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5701 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5761 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5821 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5881 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5941 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6001 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6061 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6121 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6181 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6241 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6301 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6361 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6421 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6481 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6541 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6601 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6661 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6721 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6781 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6841 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6901 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6961 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7021 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7081 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7141 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7201 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7261 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7321 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7381 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7441 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7501 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7561 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7621 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7681 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7741 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7801 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7861 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7921 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7981 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8041 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8101 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8281 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8341 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8401 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8461 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8521 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8581 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8641 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8701 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8761 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8821 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//