LOCUS       MR223777                8295 bp    DNA     circular  SYN 
DEFINITION  Smg7 (Myc-DDK-tagged) - Mouse Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) (Smg7), transcript variant 2
ACCESSION   NM_001160256
VERSION     NM_001160256.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4452..4543 
                     /label="Myc-DDK"  
     misc_feature    4573..5158 
                     /label="PolyA signal"  
     misc_feature    4630..4649 
                     /label="Restriction Enzyme"  
     misc_feature    5434..6106 
                     /label="ColE1"  
     misc_feature    6536..7330 
                     /label="Kan/Neo"  
     misc_feature    7365..7667 
                     /label="SV40 ori"  
     misc_feature    7753..8230 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4434..4440 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4434 
                     /label="Smg7"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCTGCAG AGCGCGCAGT ATCTCCGGCA GGCAGAAGTC CTGAAGGCTG 
     1081 AAATGACAGA TTCTAAGCTG GGTCCGGCTG AGGTCTGGAC ATCCAGGCAA GCTCTGCAGG 
     1141 ACTTGTACCA GAAAATGCTA GTTACTGATC TGGAATACGC TTTAGACAAG AAAGTAGAAC 
     1201 AGGATCTCTG GAATCATGCC TTTAAGAATC AGATCACAAC ACTGCAAGGC CAAGCCAAGA 
     1261 ATCGAGCAAA CCCAAATCGA AGTGAAGTCC AGGCAAACCT TTCTCTGTTC CTAGAGGCAG 
     1321 CTAGCGGCTT CTATACTCAG TTATTACAGG AACTGTGTAC GGTGTTTAAT GTAGATTTGC 
     1381 CATGCCGTGT GAAGTCTTCC CAGTTGGGAA TTATTAGCAA TAAACAGACG CACAGCAGCA 
     1441 CCATAGTGAA GCCACAGTCT AGCTCCTGCT CCTACATTTG CCAACACTGC CTCGTCCACC 
     1501 TTGGAGACAT TGCTCGATAC AGAAATCAGA CTAGCCAGGC AGAGTCCTAC TATAGGCATG 
     1561 CAGCTCAGCT TGTCCCCTCC AATGGTCAGC CTTACAATCA GTTGGCTATC TTAGCTTCTT 
     1621 CCAAAGGAGA CCACCTGACC ACAATTTTCT ACTACTGCAG AAGCATTGCT GTGAAATTCC 
     1681 CTTTCCCAGC TGCCTCCACT AATCTACAAA AAGCACTTTC TAAAGCACTG GAAAGCCGGG 
     1741 ATGAGTTGAA AACCAAGTGG GGTGTTTCTG ACTTCATCAA GGCCTTTATT AAATTCCACG 
     1801 GTCATGTATA CCTGAGTAAG AGTTTGGAAA AGCTGAGCCC TCTTCGAGAG AAGTTGGAAG 
     1861 AACAGTTTAA GAGACTGCTG TTCCAAAAGG CTTTCAACTC TCAACAGTTA GTTCATGTCA 
     1921 CTGTCATTAA CCTGTTTCAA CTTCATCATC TTCGTGACTT TAGCAATGAA ACAGAGCAAC 
     1981 ACAGTTACAG CCAAGATGAA CAGCTGTGTT GGACACAGTT GCTGGCCCTC TTTATGTCTT 
     2041 TTCTCGGCAT CCTGTGCAAA TGTCCTCTCC AGAATGATTC TCAGGAGTCC AACAATGCCT 
     2101 ACCCCCTTCC TGCAGTCAAG GTCTCCATGG ACTGGCTAAG ACTCCGACCT AGAGTCTTTC 
     2161 AAGAAGCAGT GGTGGATGAA AGACAGTACA TTTGGCCCTG GCTAATTTCT CTTCTAAATA 
     2221 GTTTCCATCC CCGTGAAGAT GATCTCTCAA ATACTAATGC CACACCACTT CCAGAGGAGT 
     2281 TTGAATTACA AGGATTCTTG GCTTTAAGAC CTTCTTTCAG GAACTTGGAT TTTTCCAAAG 
     2341 GCCATCAGGG TATTACAGGA GACAAAGAGG GTCAACAACG ACGAATACGA CAGCAGCGTT 
     2401 TGATCTCCAT AGGGAAATGG ATTGCTGATA ACCAGCCACG GCTGATTCAG TGTGAAAATG 
     2461 AGGTAGGGAA ATTGTTATTT ATCACAGAAA TCCCAGAATT AATACTAGAA GACCCCAGTG 
     2521 AAGCCAAAGA GAACCTCATC CTACAAGAAA CATCTGTGGT AGAGTCACTG GCTACGGATG 
     2581 GGAGCCCAGG ACTGAAATCA GTGCTGTCTA CAGGCCGAAA TCCAAGCAAC AGCTGTGACT 
     2641 CAGGAGAGAA ACCAGTGGTC ACCTTTAAAG AGAACATTAA GCCACGAGAA GTGAACCAAG 
     2701 GAAGAAGCTT TCCTCCCAAA GAGGTAAAAT CCCAGACAGA ACTAAGAAAG ACTCCAGTGT 
     2761 CTGAAGCCAG GAAAACGCCT GTCACTCAAA CTCCAAGTCA AACGAGTAAT TCTCAGTTCA 
     2821 TCCCCATCCA TCACCCTGGA GCCTTCCCTC CTCTTCCCAG CCGACCAGGA TTCCCGCCCC 
     2881 CAACATATGT TATCCCCCCT CCTGTGGCAT TTTCTATGGG CTCAGGTTAC ACCTTCCCAG 
     2941 CTGGTGTTTC TGTCCCAGGA ACCTTTCTTC AGTCTACAGC TCACTCTCCA GCAGGAAACC 
     3001 AGGTGCAAGC TGGGAAACAG TCCCACATTC CTTACAGCCA GCAACGGCCC TCTGGACCAG 
     3061 GGCCAATGAA CCAGGGACCT CAACAGTCAC AGCCACCTTC CCAGCCACCC CTTACATCTT 
     3121 TACCAGCTCA GCCAACAGCA CAGTCTACAA GCCAATTGCA GGTTCAAGCT CTAGCTCAGC 
     3181 AACAGCAATC CCCTACAAAA GTCATACCAG CTTTGGGGAA AAGCCCGCCT CACCACTCTG 
     3241 GATTCCAGCA GTATCAACAG GCAGATGCCT CCAAACAGCT GTGGAATCCC CCTCAGGTTC 
     3301 AAAGCCCACT AGGGAAAATT ATGCCTGTGA AACAGTCCTA CTACCTTCAG ACCCAAGACC 
     3361 CTATAAAACT GTTTGAGCCG TCATTGCAAC CTCCTGTAAT ACAGCAGCAG CCTCTAGAGA 
     3421 AAAAAATGAA GCCTTTCCCC ATGGAGCCAT ATAACCATAA TCCCTCAGAA GTCAAGGTCC 
     3481 CAGAGTTCTA CTGGGATTCT TCCTACAGCA TGGCCGATAA CAGAGCAGTA ATGGCTCAGC 
     3541 AACCAAATAT GGACCGCAGG AGCAAACGGT CACCTGGAGT CTTCCGTCCA GAACAGGATC 
     3601 CTGTGCCCAG GATGCCATTT GAGGACCCCA AGAGCTCCCC TCTGCTTCCT CCGGACCTGT 
     3661 TAAAGAGTCT GGCTGCCTTG GAGGAAGAGG AAGAGCTGAT CTTTTCTAAC CCTCCTGATC 
     3721 TTTACCCAGC TCTGCTGGGT CCTCTCGCCT CTCTTCCTGG ACGAAGCCTC TTTAAATCCT 
     3781 TATTGGAGAA GCCTTCTGAG CTCATGTCAC ATTCATCTTC TTTCCTGTCC CTCACGGGGT 
     3841 TCTCTGTCAA TCAGGAAAGA TATCCAAACA GCAGTATGTT CAATGAAGTG TATGGAAAAA 
     3901 ACCTGACAAC CAGCTCCAAG GCAGAACTGA ACCCCTCAGT CGCCTCCCAG GAAACATCAC 
     3961 TGTACTCCCT TTTTGAAGGG ACCCCATGGT CTCCATCACT TCCTGCCAGT TCAGATCATT 
     4021 CGACACCAGC CAGCCAGTCT CCTCATTCCT CTAACCCAAG CAGCCTGCCC AGTTCTCCTC 
     4081 CAACGCATAA CCATAATTCT GCTCCATTCT CTAATTTTGG ACCCATTGGG ACTCCAGATA 
     4141 ACAGGGATAG GCGGCCTGCA GATAGGTGGA AAACTGATAA ACCAGCCATG GGTGGTTTTG 
     4201 GTGTTGATTA TCTCTCAGCA ACGTCATCAT CTGAGAGCAG TTGGCATCAG GCTAGCACTC 
     4261 CGAGTGGCAC CTGGACAGGC CACGGCCCCT CCATGGAGGA TTCCTCTGCT GTCCTCATGG 
     4321 AAAGCCTAAA GTCTATCTGG TCCAGTTCCA TGATGCATCC TGGGCCCTCC GCTCTGGAGC 
     4381 AGTTGTTAAT GCAGCAGAAG CAGAAACAGC AGCGGGGACA AGGTGCCATG AACCCTCCAC 
     4441 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4501 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4561 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4621 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4681 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4741 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4801 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4861 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4921 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4981 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5041 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5101 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5161 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5221 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5281 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5341 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5401 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5461 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5521 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5581 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5641 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5701 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5761 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5821 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5881 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5941 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6001 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6061 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6121 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6181 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6241 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6301 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6361 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6421 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6481 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6541 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6601 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6661 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6721 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6781 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6841 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6901 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6961 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7021 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7081 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7141 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7201 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7261 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7321 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7381 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7441 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7501 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7561 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7621 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7681 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7741 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7801 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7861 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7921 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7981 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8041 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8101 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8161 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8221 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8281 TTTAACGCGA ATTTT
//