LOCUS       MR223721                8079 bp    DNA     circular  SYN 
DEFINITION  Pdzrn3 (Myc-DDK-tagged) - Mouse PDZ domain containing RING finger 3 (Pdzrn3)
ACCESSION   NM_018884
VERSION     NM_018884.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4234..4325 
                     /label="Myc-DDK"  
     misc_feature    4355..4940 
                     /label="PolyA signal"  
     misc_feature    4412..4431 
                     /label="Restriction Enzyme"  
     misc_feature    5216..5888 
                     /label="ColE1"  
     misc_feature    6318..7112 
                     /label="Kan/Neo"  
     misc_feature    7147..7449 
                     /label="SV40 ori"  
     misc_feature    7535..8012 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4209..4216 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4209 
                     /label="Pdzrn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTTTCGAG TTGGATCGCT TCGACGGCGA CGTGGACCCT GATCTGAAGT 
     1081 GCGCCTTGTG CCACAAGGTC CTGGAGGACC CGCTGACCAC CCCGTGCGGC CACGTATTCT 
     1141 GCGCCGGCTG CGTGCTGCCC TGGGTAGTGC AGGAGGGCAG CTGCCCCGCG CGTTGTCGCG 
     1201 GTCGCCTATC GGCCAAGGAG CTCAACCACG TCCTGCCCCT CAAGCGTCTC ATCCTCAAGT 
     1261 TGGACATCAA GTGCGCGCAC GCGGCGCGGG GCTGCGGCCG GGTGGTCAAG TTGCAGGACT 
     1321 TGCCCGAGCA CCTGGAGCGC TGCGACTTCG CGCCCGCGCG CTGCCGCCAC GCGGGCTGCG 
     1381 GCCAGCTGCT GCTGCGACGC GACGTGGAGG CCCACATGCG CGACGCGTGC GACGCGCGGC 
     1441 CCGTGGGTCG CTGCCAGGAG GGCTGCGGGC TGCCGCTGAC GCACGGCGAG CAGCGGGCGG 
     1501 GCGGCCACTG CTGCGCGCGG GCTCTGCGGG CGCACAACGG AGCGCTGCAG GCCCGCCTGG 
     1561 GCGCGCTGCA CAAGGCGCTC AAGAAGGAGG CGCTGCGGGC CGGCAAGCGC GAGAAGTCGC 
     1621 TGGTGGCGCA GCTGGCCGCC GCGCAGCTCG AACTGCAGAT GACCGCGCTG CGCTACCAGA 
     1681 AGAAGTTCAC CGAGTACAGC GCGCGCCTCG ACTCGCTCAG CCGCTGCGTG GCCGCGCCGC 
     1741 CAGGCGGCAA GGGAGAAGAG ACCAAAAGCC TGACTCTTGT CCTGCATCGG GACTCTGGCT 
     1801 CCCTGGGATT CAATATCATT GGCGGCCGAC CCTGTGTGGA CAATCAAGAT GGATCCTCCA 
     1861 GTGAAGGAAT CTTTGTATCC AAAATAGTTG ACAGCGGGCC TGCTGCCAAG GAAGGAGGCC 
     1921 TGCAAATTCA TGACAGGATT ATTGAGGTCA ACGGCAAAGA CTTATCCCGA GCAACTCATG 
     1981 ACCAGGCTGT GGAAGCTTTC AAGACAGCCA AGGAGCCCAT TGTGGTGCAG GTGTTGAGGA 
     2041 GGACACCTCG AACCAAGATG TTCACGCCTG CCTCAGAGTC GCAGCTGGTA GACACGGGCA 
     2101 CCCAAACCGA CATCACCTTT GAGCACATCA TGGCCCTGAC AAAGATGTCT TCTCCCAGCC 
     2161 CACCTGTGCT AGACCCCTAC CTGCTGCCTG AAGAGCATCC CGCATCCCAT GACTACTATG 
     2221 ATCCCAATGA CTACATGGGG GATATCCATC AGGACATGGA CAGAGAAGAG CTGGAGCTGG 
     2281 AAGAAGTGGG CCTCTACAGG ATGAACAGCC AGGACAAGCT GGGTCTCACT GTGTGCTACC 
     2341 GGACGGATGA CGAAGATGAC ATTGGGATAT ATATAAGTGA GATTGACCCT AACAGCATTG 
     2401 CAGCCAAGGA TGGACGGATC CGTGAAGGAG ATCGCATCAT CCAGATTAAT GGCATCGAAG 
     2461 TACAGAACCG TGAAGAGGCC GTGGCGCTTC TAACCAGTGA AGAAAACAAG AACTTTTCAT 
     2521 TGCTGATTGC AAGGCCTGAG CTCCAGCTGG ATGAGGGCTG GATGGACGAC GACAGGAACG 
     2581 ACTTCCTGGA TGACTTACAC ATGGACATGC TGGAGGAACA GCATCACCAG GCCATGCAGT 
     2641 TCACCGCCAG TGTGCTCCAG CAGAAGAAGC ATGAGGAAGA CGGTGGGACC ACAGACACAG 
     2701 CCACCATCTT ATCCAACCAG CACGAGAAAG ACAGTGGTGT CGGGCGAACG GATGAGAGCA 
     2761 CCCGCAATGA TGAGAGCTCA GAACAGGAGA ATAACGGTGA AGATGCTACG GCATCCGCCA 
     2821 ACCCGCTGGC AGGCCAGAGG AAGCTGACCT GTAGCCAGGA CACCCTGGGC AGTGGCGACC 
     2881 TGCCTTTCAG CAATGAGTCC TTCATCTCTG CGGACTGTAC CGATGTGGAC TACCTGGGCA 
     2941 TCCCAGAGGA TGAGTGCGAG CGTTTTCGCG AACTGCTGGA GCTCAAGTGC CAAGTGCAGA 
     3001 GCGCCAGCCC CTACAGCCTG TACTACCCTA GCAGCCCACT GGATGCTGCT GGCAAGAGTG 
     3061 ACCCCGAGAG CGTGGACAAG GAGCTGGAGC TACTCAATGA GGAGTTGCGC AGCATCGAGC 
     3121 TGGAGTGCCT GAGCATCGTG CGCGCGCACA AGATGCAACA GCTCAAGGAA CAGTACCGTG 
     3181 AGTCCTGGAT GCTGCACCAC AGTGGCTTCC GCAACTACAA CACCAGTGTG GACGTGCGCC 
     3241 GCCATGAGCT CTCGGACATC ACTGAGCTGC CGGAGAAGTC AGACAAGGAT AGTTCAAGCG 
     3301 CCTACAACAC GGGGGAGAGC TGCAGAAGTA CCCCACTCAC CCTGGAGATC TCTCCGGACA 
     3361 ACTCCCTGCG GAGAGTAGCC GAGGGCAGTA GTGAAGGGGC CACAGCTAAC ATCGAAGCTT 
     3421 ACAGGCCATC CCCCAAGAAT CTGCTCGCCA TCACTGAGGA CCCTGAAGTA AGCACCCCAA 
     3481 GCTATAACCC TAGTGCCAAA GAGCTGGACC CCAGCCAGGC TTTGGAGATC AAAGAACGCC 
     3541 GAGGTAGCGA TGGCAGCAGG AGCCCCACCG CCAGCCCAAA GCTGGGCAAT GCCTACCTGC 
     3601 CCTCATACCA CCACTCCCCC TACAAACACG CGCACATCCC AGCTCACGCG CAACACTACC 
     3661 AGAGCTACAT GCACCTGATC CAGCAGAAGT CAGCAGTGGA GTATGCGCAG AGCCAGATGA 
     3721 GCCTGGTGAG CATGTGCAAG GACTTGAACT CTTCTAACTC TGTGGAACCC AGGATGGAAT 
     3781 GGAAAGTGAA GATCCGCAGT GACGGGACGC GCTACATCAC CAAGAGGCCG GTGCGTGACA 
     3841 GGCTGCTGCG GGAGCGTGCG CTGAAGATCC GCGAGGAGCG CAGTGGCCTG ACCACGGATG 
     3901 ATGACGCCAT GAGTGAGATG AAGATGGGGC GCTACTGGAG CAAGGAGGAG CGCAAGCAAC 
     3961 ATCTGGTGAA GGCCAAGGAG CAGAGGCGGA GGCGCGAGTT TATGATGCAG AGCAGGCTGG 
     4021 ATTGTCTGAA GGAGCAGCAG GCCTCCGACG ACAGGAAGGA GATGAACATC CTGGAACTGA 
     4081 GCCACAAAAA GATGATGAAG AAGAGGAACA AAAAGATCTT TGACAATTGG ATGACTATCC 
     4141 AAGAACTCTT AACCCACGGC ACAAAATCGC CAGACGGCAC TAGGGTATAC AATTCCTTCC 
     4201 TGTCGGTGAC TACTGTAAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4261 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5881 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5941 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6001 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6061 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6121 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6181 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6241 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6301 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6361 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6421 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6481 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6541 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6601 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6661 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6721 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6781 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6841 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6901 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6961 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7021 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7081 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7141 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7201 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7261 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7321 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7501 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7561 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7621 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//