LOCUS       MR223701                7824 bp    DNA     circular  SYN 
DEFINITION  Ano3 (Myc-DDK-tagged) - Mouse anoctamin 3 (Ano3), transcript variant 2
ACCESSION   NM_001128103
VERSION     NM_001128103.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3981..4072 
                     /label="Myc-DDK"  
     misc_feature    4102..4687 
                     /label="PolyA signal"  
     misc_feature    4159..4178 
                     /label="Restriction Enzyme"  
     misc_feature    4963..5635 
                     /label="ColE1"  
     misc_feature    6065..6859 
                     /label="Kan/Neo"  
     misc_feature    6894..7196 
                     /label="SV40 ori"  
     misc_feature    7282..7759 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3969 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="Ano3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCACCAC TCAGGCTCCA TTCAGTCCTT TAAACAGCAA AAAGGTATGA 
     1081 ATATAAGCAA AAGTGAGATA ACAACTGAAG CGTCATTAAA ACCATCTAGG AGATCCCTGC 
     1141 CTTGCCTGGC CCAGAGCTAT GCTCATTCAA AAAGCTTGAG TCAATCTGCT TCACTGTTTC 
     1201 AGTCAACTGA GAGTGAATCT CAGGCTCCAA CTTCTGTAAC ATTTCTTTCT GCTGATAAGC 
     1261 CTGAACATGT TACTTCGGAG GAGAGTAGAA AGGACTCTAC CCTTAAGTGT TCCTTTGCTG 
     1321 ACCTCAGTGA CTTTTGCTTG GCTCTGGGGA AAGACAAGGA TTACCTGGAT GAATCTGAAC 
     1381 ATGCCAACTA TGATCGATCT CGTCTCCTTA ATGACTTTGT TACCAAAGAT AAGCCTGCAT 
     1441 CCAAGACAAA GCTGTCTAAG AATGACATGA GTTACATAGC ATCTAGTGGA CTCTTATTCA 
     1501 AAGATGGCAA AAAGCGAATT GATTACATCC TGGTTTATCG AAAAACAAAT ATACAATATG 
     1561 ATAAAAGAAA CACATTTGAA AAGAACCTGA GAGCAGAAGG CTTGATGTTG GAGAAGGAGC 
     1621 CAGCAATTGC CAACCCCGAC ATCATGTTTA TTAAAATTCA CATTCCATGG GACACACTGT 
     1681 GCAAGTATGC AGAGAGGCTG AACATCAGGG TGCCCTTCAG GAAGAAATGT TACTATACTG 
     1741 ACCAGAAGAA CAAATCAAAG AGCAGGGTAC AAAACTATTT CAAAAGAATC AAAAAATGGA 
     1801 TGTCCCAAAA CCCAATGGTC CTTGACAAGT CAGCGTTTCC AGAGCTGGAG GAGTCGGACT 
     1861 GCTATACTGG ACCCTTCAGC AGAGCTAGGA TTCACCACTT CATAATAAAC AATAAGGACA 
     1921 CCTTCTTCAG CAATGCTACT CGAAGCAGGA TAGTTTATCA CATGCTGGAA CGCACCAAAT 
     1981 ATGAAAATGG AATATCAAAA GTGGGTATCC GTAAGCTTAT CACTAATGGT TCATACATAG 
     2041 CAGCATTTCC CCCACATGAG GGAGCCTACA AGAGTAGCCT TCCGATCAAA ACCCATGGAC 
     2101 CTCAGAATAA CAGGCATCTG TTGTATGAGC GCTGGGCACG CTGGGGAATG TGGTACAAGC 
     2161 ATCAGCCTCT GGACTTAATC AGGATGTACT TTGGTGAGAA GATTGGCTTA TACTTCGCCT 
     2221 GGCTGGGATG GTACACCGGA ATGCTGATTC CTGCTGCAGT TGTCGGCCTG TGTGTTTTCT 
     2281 TCTATGGATT AGTCACAATG AACGAAAGCC AAGTAAGCCA AGAAATCTGT AAAGCCACTG 
     2341 AAGTCTTCAT GTGTCCTCTA TGTGACAAGA ATTGCTCACT GCAGAGACTC AACGACAGCT 
     2401 GCATCTATGC CAAGGTAACA TATTTGTTTG ATAATGGAGG GACAGTTTTC TTCGCTATTT 
     2461 TTATGGCAAT ATGGGCCACA GTCTTTCTTG AGTTTTGGAA AAGGAGAAGA AGCATTCTGA 
     2521 CTTACACTTG GGACCTTATT GAATGGGAAG AAGAGGAGGA AACACTTCGC CCCCAGTTTG 
     2581 AAGCAAAATA TTACAGGATG GAGGTAATAA ACCCCATCAC GGGGAAGCCT GAGCCTCATC 
     2641 AGCCTTCATC CGACAAAGTC ACGCGACTTC TTGTGTCTGT CTCAGGAATC TTCTTCATGA 
     2701 TCTCCTTGGT CATCACAGCA GTGTTCGCAG TTGTAGTGTA CCGTCTGGTA GTCATGGAAC 
     2761 AGTTTGCATC ATTCAAGTGG AATTTCGTCA AACAGCACTG GCAGTTTGCT ACATCTGGTG 
     2821 CTGCTGTCTG TATCAATTTC ATAATCATCA TGCTGCTGAA TCTTGCTTAT GAAAAAATTG 
     2881 CATACCTCCT CACTAACTTA GAATATCCTC GAACTGAATC AGAATGGGAA AACAGCTTTG 
     2941 CCTTGAAGAT GTTCCTTTTC CAGTTTGTCA ATTTGAACAG TTCTATCTTC TATATTGCAT 
     3001 TCTTTTTGGG AAGATTCGTA GGCCACCCAG GAAAATACAA TAAACTTTTT GAGCGGTGGA 
     3061 GACTGGAGGA ATGCCATCCT AGTGGCTGTC TGATAGACCT CTGCCTCCAG ATGGGAGTCA 
     3121 TCATGTTTTT GAAGCAAATA TGGAACAACT TCATGGAGCT GGGATATCCG TTAATCCAAA 
     3181 ACTGGTGGTC ACGACACAAA ATCAAAAGGG GAATACAAGA CGCTTCCATA CCACAATGGG 
     3241 AAAATGACTG GAACCTTCAG CCTATGAACA TCCATGGGCT GATGGACGAG TACTTAGAAA 
     3301 TGGTTTTGCA ATTTGGATTC ACTACCATCT TTGTTGCTGC TTTTCCCCTG GCCCCTCTAT 
     3361 TGGCTTTGTT AAACAATATC ATAGAAATCA GATTGGATGC ATATAAATTT GTGACCCAGT 
     3421 GGAGGAGGCC TCTGCCAGCC CGAGCCACTG ACATAGGTAT CTGGCTTGGA ATTCTTGAGG 
     3481 GAATTGGCAT ATTGGCTGTG ATCACCAATG CATTTGTAAT TGCTATTACA TCTGATTACA 
     3541 TCCCACGTTT TGTCTATGAA TACAAATATG GCCCTTGTGC AAATCATGTG AAGCAGAATG 
     3601 AAAATTGCCT AAAGGGCTAT GTCAACAATA GCTTATCCTT CTTTGACCTG AGTGAACTTG 
     3661 GTATGGGAAA ATCTGGTTAC TGCAGGTACC GAGACTATAG AGGCCCACCT TGGAGTTCCA 
     3721 AACCCTATGA ATTCACCTTA CAGTACTGGC ACATCCTGGC TGCTCGATTG GCCTTCATTA 
     3781 TCGTGTTTGA GCATCTTGTT TTTGGCATTA AGTCATTCAT TGCATACCTG ATTCCTGATA 
     3841 TACCAAAGGG CCTCCGTGAG CGCATACGGC GAGAGAAATA CTTGGTCCAG GAAATGATGT 
     3901 ATGAGGCTGA ACTGGAACAT TTACAACAAC AGAGAAGAAA AAGTGGTCAG CCTATTCACC 
     3961 ATGAATGGCC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4021 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4081 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4141 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4201 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4261 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4321 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4381 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4441 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4501 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4561 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4621 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4681 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4741 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4801 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4861 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4921 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4981 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5041 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5161 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5221 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5281 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5341 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5461 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5521 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5581 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5641 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5701 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5761 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5821 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5881 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5941 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6001 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6061 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6121 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6181 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6241 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6301 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6361 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6421 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6481 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6541 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6601 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6661 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6721 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6781 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6841 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6901 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6961 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7021 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7081 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7141 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7261 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7321 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7381 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7441 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7501 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7561 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7621 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7681 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7741 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7801 TAACAAAAAT TTAACGCGAA TTTT
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