LOCUS       MR223642                7407 bp    DNA     circular  SYN 
DEFINITION  Tas1r1 (Myc-DDK-tagged) - Mouse taste receptor, type 1, member 1 (Tas1r1)
ACCESSION   NM_031867
VERSION     NM_031867.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3564..3655 
                     /label="Myc-DDK"  
     misc_feature    3685..4270 
                     /label="PolyA signal"  
     misc_feature    3742..3761 
                     /label="Restriction Enzyme"  
     misc_feature    4546..5218 
                     /label="ColE1"  
     misc_feature    5648..6442 
                     /label="Kan/Neo"  
     misc_feature    6477..6779 
                     /label="SV40 ori"  
     misc_feature    6865..7342 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3546..3552 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3546 
                     /label="Tas1r1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTTTCTGG GCAGCTCACC TGCTGCTCAG CCTGCAGCTG GCCGTTGCTT 
     1081 ACTGCTGGGC TTTCAGCTGC CAAAGGACAG AATCCTCTCC AGGTTTCAGC CTCCCTGGGG 
     1141 ACTTCCTCCT GGCAGGCCTG TTCTCCCTCC ATGCTGACTG TCTGCAGGTG AGACACAGAC 
     1201 CTCTGGTGAC AAGTTGTGAC AGGTCTGACA GCTTCAACGG CCATGGCTAT CACCTCTTCC 
     1261 AAGCCATGCG GTTCACCGTT GAGGAGATAA ACAACTCCAC AGCTCTGCTT CCCAACATCA 
     1321 CCCTGGGGTA TGAACTGTAT GACGTGTGCT CAGAGTCTTC CAATGTCTAT GCCACCCTGA 
     1381 GGGTGCTCGC CCAGCAAGGG ACAGGCCACC TAGAGATGCA GAGAGATCTT CGCAACCACT 
     1441 CCTCCAAGGT GGTGGCACTC ATTGGGCCTG ATAACACTGA CCACGCTGTC ACCACTGCTG 
     1501 CCCTGCTGAG CCCTTTTCTG ATGCCCCTGG TCAGCTATGA GGCGAGCAGC GTGATCCTCA 
     1561 GTGGGAAGCG CAAGTTCCCG TCCTTCTTGC GCACCATCCC CAGCGATAAG TACCAGGTGG 
     1621 AAGTCATAGT GCGGCTGCTG CAGAGCTTCG GCTGGGTCTG GATCTCGCTC GTTGGCAGCT 
     1681 ATGGTGACTA CGGGCAGCTG GGCGTACAGG CGCTGGAGGA GCTGGCCACT CCACGGGGCA 
     1741 TCTGCGTCGC CTTCAAGGAC GTGGTGCCTC TCTCCGCCCA GGCGGGTGAC CCAAGGATGC 
     1801 AGCGCATGAT GCTGCGTCTG GCTCGAGCCA GGACCACCGT GGTCGTGGTC TTCTCTAACC 
     1861 GGCACCTGGC TGGAGTGTTC TTCAGGTCTG TGGTGCTGGC CAACCTGACT GGCAAAGTGT 
     1921 GGATCGCCTC CGAAGACTGG GCCATCTCCA CGTACATCAC CAATGTGCCC GGGATCCAGG 
     1981 GCATTGGGAC GGTGCTGGGG GTGGCCATCC AGCAGAGACA AGTCCCTGGC CTGAAGGAGT 
     2041 TTGAAGAGTC CTATGTCCAG GCAGTGATGG GTGCTCCCAG AACTTGCCCA GAGGGGTCCT 
     2101 GGTGCGGCAC TAACCAGCTG TGCAGGGAGT GTCACGCTTT CACGACATGG AACATGCCCG 
     2161 AGCTTGGAGC CTTCTCCATG AGCGCTGCCT ACAATGTGTA TGAGGCTGTG TATGCTGTGG 
     2221 CCCACGGCCT CCACCAGCTC CTGGGATGTA CCTCTGGGAC CTGTGCCAGA GGCCCAGTCT 
     2281 ACCCCTGGCA GCTTCTTCAG CAGATCTACA AGGTGAATTT CCTTCTACAT AAGAAGACTG 
     2341 TAGCATTCGA TGACAAGGGG GACCCTCTAG GTTATTATGA CATCATCGCC TGGGACTGGA 
     2401 ATGGACCTGA ATGGACCTTT GAGGTCATTG GTTCTGCCTC ACTGTCTCCA GTTCATCTAG 
     2461 ACATAAATAA GACAAAAATC CAGTGGCACG GGAAGAACAA TCAGGTGCCT GTGTCAGTGT 
     2521 GTACCAGGGA CTGTCTCGAA GGGCACCACA GGTTGGTCAT GGGTTCCCAC CACTGCTGCT 
     2581 TCGAGTGCAT GCCCTGTGAA GCTGGGACAT TTCTCAACAC GAGTGAGCTT CACACCTGCC 
     2641 AGCCTTGTGG AACAGAAGAA TGGGCCCCTG AGGGGAGCTC AGCCTGCTTC TCACGCACCG 
     2701 TGGAGTTCTT GGGGTGGCAT GAACCCATCT CTTTGGTGCT ATTAGCAGCT AACACGCTAT 
     2761 TGCTGCTGCT GCTGATTGGG ACTGCTGGCC TGTTTGCCTG GCGTCTTCAC ACGCCTGTTG 
     2821 TGAGGTCAGC TGGGGGTAGG CTGTGCTTCC TCATGCTGGG TTCCTTGGTA GCTGGGAGTT 
     2881 GCAGCCTCTA CAGCTTCTTC GGGAAGCCCA CGGTGCCCGC GTGCTTGCTG CGTCAGCCCC 
     2941 TCTTTTCTCT CGGGTTTGCC ATTTTCCTCT CCTGTCTGAC AATCCGCTCC TTCCAACTGG 
     3001 TCATCATCTT CAAGTTTTCT ACCAAGGTAC CCACATTCTA CCACACTTGG GCCCAAAACC 
     3061 ATGGTGCCGG AATATTCGTC ATTGTCAGCT CCACGGTCCA TTTGTTCCTC TGTCTCACGT 
     3121 GGCTTGCAAT GTGGACCCCA CGGCCCACCA GGGAGTACCA GCGCTTCCCC CATCTGGTGA 
     3181 TTCTTGAGTG CACAGAGGTC AACTCTGTGG GCTTCCTGGT GGCTTTCGCA CACAACATCC 
     3241 TCCTCTCCAT CAGCACCTTT GTCTGCAGCT ACCTGGGTAA GGAACTGCCG GAGAACTATA 
     3301 ACGAAGCCAA ATGTGTCACC TTCAGCCTGC TCCTCCACTT CGTATCCTGG ATCGCTTTCT 
     3361 TCACCATGTC CAGCATTTAC CAGGGCAGCT ACCTACCCGC GGTCAATGTG CTGGCAGGGC 
     3421 TGGCCACTCT GAGTGGCGGC TTCAGCGGCT ATTTCCTCCC TAAATGCTAC GTGATTCTCT 
     3481 GCCGTCCAGA ACTCAACAAC ACAGAACACT TTCAGGCCTC CATCCAGGAC TACACGAGGC 
     3541 GCTGCGGCAC TACCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3601 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3661 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3721 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3781 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3841 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3901 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3961 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4021 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4081 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4141 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4201 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4261 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4321 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4381 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4441 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4501 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4561 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4621 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4681 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4741 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4801 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4861 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4921 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4981 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5041 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5101 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5161 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5221 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5281 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5341 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5401 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5461 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5521 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5581 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5641 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5701 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5761 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5821 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5881 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5941 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6001 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6061 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6121 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6181 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6241 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6301 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6361 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6421 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6481 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6541 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6601 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6661 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6721 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6781 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6841 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6901 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6961 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7021 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7081 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7141 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7201 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7261 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7321 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7381 ATTTAACAAA AATTTAACGC GAATTTT
//