LOCUS       MR223597                7341 bp    DNA     circular  SYN 
DEFINITION  Pomt2 (Myc-DDK-tagged) - Mouse protein-O-mannosyltransferase 2 (Pomt2)
ACCESSION   NM_153415
VERSION     NM_153415.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3498..3589 
                     /label="Myc-DDK"  
     misc_feature    3619..4204 
                     /label="PolyA signal"  
     misc_feature    3676..3695 
                     /label="Restriction Enzyme"  
     misc_feature    4480..5152 
                     /label="ColE1"  
     misc_feature    5582..6376 
                     /label="Kan/Neo"  
     misc_feature    6411..6713 
                     /label="SV40 ori"  
     misc_feature    6799..7276 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3480..3486 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3480 
                     /label="Pomt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCTACGCC TCAGGCCGCC TCTTAGCTGC CCGGGCGGCC ACCACACTTT 
     1081 CCGCCCCGCC CAGAGCCCGG GGCCCGGCGC TCCGCGGCAA ACGGAGGGAA CTACAAATCC 
     1141 CATGGCACCT CGAGACGCCT TCTTATGACC CCTTGACGGG GCAGCGGACA AGACCCGGCG 
     1201 TGCCTCCGGC CCGGAGGGTG ATCCTTCGGA AGGGGAGGAT GCCGCCGGCC ATAGGCGGTG 
     1261 GCCTAGCGGG CTCCGAGCTG CGTCCCCGTA GGGGCCGCTG TGTGCCGCAG GCTGCCAGAG 
     1321 CCGTGAGCCG AGACGTGGTC CCCCAGGCTG CAGCACGGAA ACTTAAACGG CCTGCTTGGA 
     1381 GCTCGCGGCG CTTCCAGGCG GCAGGGTGGT GGGCCACGTT GGCTGTGGTG ACGCTGCTGT 
     1441 CCTTTGCCAC CCGCTTTCAC CGGCTGGACC AGCCTGCTCA CATCTGTTGG GATGAGACTC 
     1501 ACTTTGGAAA AATGGGAAGT TACTATATTA ACCGCACCTT TTTCTTTGAT GTGCATCCAC 
     1561 CACTGGGAAA GATGCTGATC GGTCTTGCTG GCTACCTGAG CGGGTATGAT GGTACCTTTC 
     1621 TGTTCCAGAA GCCTGGGGAC AGATACGAGC ACCACAGCTA CATGGGAATG AGAGGATTCT 
     1681 GTGCTTTCCT GGGTTCCTGG CTGATCCCCT TTGCCTACCT CACCGTACTG GATCTGTCCA 
     1741 AGTCCTTCCC AGCAGCACTG CTCACCGCTG CCCTTCTCAC CTTTGACACA GGATGCCTCA 
     1801 CTCTATCCCA GTACATCCTC CTTGACCCCA TCCTGATGTT CTTCATCATG GCGGCCATGC 
     1861 TGAGCATGGT CAAGTACAAC TCCTGTGCTA ACAGGCCTTT CTCTGCCCCC TGGTGGTTCT 
     1921 GGCTCAGCCT GACTGGCATT AGTCTCGCTG GTGCTTTAGG GGTCAAATTT GTTGGCCTCT 
     1981 TTATCATCGT GCAAGTGGGG TTGAACACTA TTTCAGACCT CTGGCATCTG TTTGGAGACC 
     2041 TCAGCCTTTC ACTGGTGACT GTGGGGAAGC ACCTAACTGC TCGCATCCTG TGCCTCATAG 
     2101 TGCTGCCTTT GGTGCTCTAT GTGACCATTT TTGCTGTTCA TGTCATGGTG CTGAATAAAA 
     2161 GTGGTCCTGG TGATGGCTTC TTCAGTTCTG CCTTTCAAGC CCGGCTTTCA GGGAACAGCC 
     2221 TTCACAATGC TTCTATCCCT GAACACCTGG CGTATGGCTC TGTAATCACT GTGAAGAATC 
     2281 TCCGGATGGC CATTGGCTAC CTGCACTCTC ATAGGCACCT CTACCCTGAG GGCATTGGTG 
     2341 CTCGCCAGCA GCAGGTTACC ACCTATTTGC ATAAGGACTA CAACAACTTG TGGATTATCA 
     2401 AGAAGTATAA TGCCAACACA GATCCCCTAG ACCCTTCATT CCCAGTGGAG TTTGTCAGAC 
     2461 ATGGAGACAT CATACGACTA GAACACAAAG AAACTACTCG GAACCTGCAC AGTCACTATC 
     2521 ATGAGGCTCC CCTGACCCGG AAGCACTATC AGGTCACTGG CTATGGCATA AATGGGACAG 
     2581 GGGACTCAAA TGACTTCTGG CGGATTGAAG TTGTAAATAG AAAATTTGGG AACCGGATCA 
     2641 AGGTACTGAG AAGTCGAATT CGCCTCATCC ATTTGGTCAC AGGTTGTGTC CTGGGATCGT 
     2701 CAGGAAAGAT TCTGCCTAAG TGGGGCTGGG AGCAGTTGGA AGTTACCTGT ACTCCGTACC 
     2761 TAAAAGAAAC CACTAACTCC ATTTGGAACA TAGAAGAGCA TATCAATCCC AAATTGCCAA 
     2821 ACATCAGCCT GGATGTGCTG CAGCCCAGTT TTCCTGAGAT CTTGCTGGAG TCTCACATGG 
     2881 TCATGATCCG GGGAAATAAT GGCCTCAAAC CCAAGGACAA TGAGTTCACA TCCAAGCCCT 
     2941 GGCACTGGCC TATCAATTAT CAGGGCCTGC GCTTCTCAGG GGCCAATGAC ACGGACTTCC 
     3001 GAGTCTATCT GCTCGGCAAC CCTGTGGTCT GGTGGCTGAA TCTGGTGAGC ATTGTCCTCT 
     3061 ACCTCCTCTC GGGGAGCACC ATTGCTGTGG CTATGCAGAG AGGGATACAA CTGCCTGCAG 
     3121 AGTTGCAAGG GCTGACCAAG GTGCTGCTGC GAGGAGGTGG CCAGCTGCTC CTGGGATGGA 
     3181 TGCTCCATTA CTTCCCTTTC TTCTTGATGG GCCGGATCCT CTACTTCCAC CACTACTTCC 
     3241 CAGCCATGCT CTTTTCCAGC ATGTTGACAG GTATCCTATG GGATACTCTC CTGCGGCTCT 
     3301 GTGCCTGGGG CTTGGCCCCT TCTCCCCTGG GTAGGAGGAT ACATGCGGTG GGAATCCTGA 
     3361 GCCTGCTTCT GACAACTGCC TACAGCTTCT ACCTCTTCCA CCCTCTGGCT TATGGGATGG 
     3421 TTGGTCCCCT AGCCCAGGAA CCTGAGAGTC CAATGGCAGG ACTGAGGTGG CTGGAATCAT 
     3481 GGGACTTTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3541 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3601 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3661 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3721 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3781 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3841 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     3901 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     3961 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4021 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4081 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4141 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4201 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4261 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4321 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4381 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4441 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4501 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4561 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4621 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4681 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4741 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4801 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4861 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4921 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4981 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5041 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5101 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5161 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5221 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5281 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5341 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5401 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5461 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5521 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5581 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5641 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5701 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5761 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5821 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     5881 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     5941 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6001 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6061 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6121 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6181 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6241 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6301 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6361 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6421 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6481 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6541 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6601 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6661 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6721 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6781 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6841 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     6901 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     6961 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7021 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7081 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7141 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7201 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7261 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7321 CAAAAATTTA ACGCGAATTT T
//