LOCUS       MR223553                9189 bp    DNA     circular  SYN 
DEFINITION  Ptpru (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, receptor type, U (Ptpru), transcript variant 1
ACCESSION   NM_011214
VERSION     NM_011214.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5346..5437 
                     /label="Myc-DDK"  
     misc_feature    5467..6052 
                     /label="PolyA signal"  
     misc_feature    5524..5543 
                     /label="Restriction Enzyme"  
     misc_feature    6328..7000 
                     /label="ColE1"  
     misc_feature    7430..8224 
                     /label="Kan/Neo"  
     misc_feature    8259..8561 
                     /label="SV40 ori"  
     misc_feature    8647..9124 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5328..5334 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5328 
                     /label="Ptpru"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCGGGCT CAGGCTCTGG TCCTGGCGCT CACCTTCCAG TTCTGCGCGC 
     1081 CTGAGACCGA GACTCCCGCA GCTGGCTGCA CCTTCGAGGA GGCGAGTGAC CCGGTCGTGC 
     1141 CCTGCGAGTT CAGCCAGGCT CAGTATGACG ACTTCCAATG GGAGCAAGTG CGGATCCACC 
     1201 CCGGCACCCG GACCCCTGAA GACCTGCCCC ATGGTGCCTA CTTGATGGTC AATGCTTCTC 
     1261 AGCATGCCCC AGGTCAGAGG GCCCACATCA TCTTCCAGAC CCTGAGCGAG AACGACACCC 
     1321 ATTGTGTGCA GTTCAGCTAC TTCCTGTACA GCAGGGATGG GCACAGCCCA GGCACCCTGG 
     1381 GGGTCTACGT GCGCGTGAAT GGGGGCCCTC TGGGCAGTGC CGTGTGGAAT ATGACCGGAT 
     1441 CCCACGGCCG TCAGTGGCAC CAGGCTGAGC TGGCTGTCAG CACCTTCTGG CCCAATGAGT 
     1501 ATCAGGTGCT GTTTGAGGCC CTCATCTCCC CAGACCACAA GGGCTACATA GGCTTAGACG 
     1561 ACATCTTGCT CTTCAGCTAT CCCTGCGCAA AGGCCCCTCA CTTCTCCCGC CTTGGGGACG 
     1621 TGGAGGTCAA TGCAGGCCAG AACGCATCCT TCCAATGCAT GGCAGCAGGC AGAGCCGCAG 
     1681 AGGCAGAACA CTTCTTCCTG CAGCGTCAGA GTGGAGTGCT GGTGCCTGCG GCCGGGGTGC 
     1741 GGCACATCAG CCACCGTCGC TTCCTGGCCA CTTTTCCGCT GGCCTCGGTA GGCCGCTCAG 
     1801 AGCAGGATCT GTACCGTTGC GTGTCCCAGG CCCCGCGTGG TGCTGGCGTC TCCAACTTTG 
     1861 CAGAGCTCAT CGTCAAAGAG CCTCCCACCC CCATCGCGCC CCCACAGCTG CTGCGTGCAG 
     1921 GCCCCACCTA CCTCATTATC CAGCTCAACA CCAACTCCAT CATTGGCGAC GGGCCGATCG 
     1981 TGCGCAAGGA GATCGAGTAC CGCATGGCAC GGGGCCCGTG GGCCGAGGTG CACGCTGTCA 
     2041 ACCTGCAGAC CTACAAGCTG TGGCATCTGG ACCCAGACAC TGAGTATGAA ATCAGCGTGC 
     2101 TGCTCACACG CCCGGGAGAT GGAGGCACAG GCCGCCCTGG GCCACCACTG ATCAGCCGGA 
     2161 CCAAGTGCGC AGAGCCCACG AGGGCCCCCA AAGGTCTGGC TTTTGCTGAG ATCCAGGCTC 
     2221 GCCAGCTGAC CCTGCAGTGG GAGCCCCTGG GCTATAATGT CACACGTTGT CATACCTACG 
     2281 CTGTGTCCCT TTGCTATCGC TACACCCTGG GCGGCAGCCA CAACCAGACC ATCCGGGAGT 
     2341 GTGTGAAGAT GGAGCGGGGT GCCAGCCGCT ACACCATCAA GAATCTGCTG CCATTCAGAA 
     2401 ACATCCACGT GCGTCTGATT CTCACAAACC CTGAGGGGCG CAAGGAGGGC AAGGAGGTCA 
     2461 CCTTCCAGAC AGATGAAGAT GTGCCTGGTG GGATTGCAGC TGAGTCCCTA ACCTTCACTC 
     2521 CACTGGAGGA CATGATCTTT CTCAAGTGGG AGGAGCCCCA GGAGCCCAAT GGCCTCATCA 
     2581 CTCAGTATGA GATCAGCTAC CAAAGCATTG AGTCCTCAGA CCCAGCAGTG AACGTGCCCG 
     2641 GCCCGAGACG CACCATCTCC AAACTCCGGA ATGAGACTTA CCACGTCTTC TCCAACCTGC 
     2701 ATCCCGGCAC CACGTATCTG TTCTCCGTGC GTGCTCGGAC GAGCAAGGGC TTCGGCCAGG 
     2761 CGGCTCTCAC TGAGATAACC ACCAACATCT CAGCTCCCAG CTTTGATTAT GCCGACATGC 
     2821 CGTCACCCCT GGGCGAGTCC GAGAACACCA TCACTGTGCT GTTGAGGCCG GCCCAGGGCC 
     2881 GAGGAGCCCC CATCAGCGTC TACCAGGTGG TTGTGGAGGA AGAGCGGCCA CGGCGCTTGC 
     2941 GGCGGGAGCC CGGAGCTCAG GACTGCTTCT CGGTACCTCT GACCTTTGAG ACGGCCCTGG 
     3001 CTCGCGGCCT GGTGCACTAC TTTGGGGCTG AACTGGCTGC CAGCAGCCTG CTTGAGGCCA 
     3061 TGCCCTTCAC CGTGGGTGAC AACCAGACCT ATCGTGGCTT CTGGAACCCA CCGCTTGAGC 
     3121 CCAGAAAGGC CTATCTCATC TATTTCCAGG CAGCAAGCCA CCTGAAAGGG GAAACCCGAC 
     3181 TGAACTGCAT CCGAATTGCC AGGAAAGCTG CGTGCAAGGA GAGCAAGCGA CCCCTCGAAG 
     3241 TGTCCCAGAG ATCGGAGGAG ATGGGGCTCA TCCTGGGCAT CTGTGCAGGT GGTCTTGCCG 
     3301 TCCTCATTCT CCTCCTGGGG GCCATCATTG TCATCATCCG CAAAGGGAAG CCAGTGAACA 
     3361 TGACGAAAGC CACGGTCAAC TACCGCCAGG AGAAGACTCA CATGATGAGT GCCGTGGACC 
     3421 GCAGCTTCAC AGATCAGAGT ACTCTGCAGG AGGATGAGCG GTTGGGTCTG TCCTTTATGG 
     3481 ATGCTCCTGG CTATAGTCCT CGTGGAGACC AGCGAAGCGG TGGTGTCACC GAGGCCAGCA 
     3541 GCCTCCTGGG GGGTTCTCCA AGGCGCCCAT GCGGCCGGAA GGGTTCTCCG TATCATACCG 
     3601 GGCAGCTCCA CCCTGCAGTC CGAGTGGCTG ACCTTCTACA GCACATCAAC CAGATGAAGA 
     3661 CAGCCGAGGG CTACGGCTTC AAGCAGGAGT ACGAGAGTTT CTTTGAGGGC TGGGACGCCA 
     3721 CCAAGAAGAA AGACAAGCTC AAGGGCGGCC GACAGGAGCC AGTGTCTGCC TATGATCGAC 
     3781 ACCATGTGAA ACTACACCCG ATGCTGGCAG ACCCTGATGC CGACTACATC TCTGCCAACT 
     3841 ACATAGACGG CTACCACAGG TCAAACCACT TCATAGCCAC TCAAGGGCCA AAGCCTGAGA 
     3901 TGATCTACGA TTTCTGGCGC ATGGTGTGGC AGGAACAGTG TGCGAGCATC GTCATGATCA 
     3961 CCAAGCTGGT AGAGGTGGGC AGGGTGAAGT GTTCTCGCTA CTGGCCTGAG GACTCAGACA 
     4021 TGTATGGGGA CATCAAGATC ACGCTGGTAA AGACAGAGAC ACTGGCTGAG TATGTGGTGC 
     4081 GCACCTTTGC CCTGGAGCGG AGAGGTTACT CAGCCCGGCA TGAGGTCCGC CAGTTCCATT 
     4141 TCACAGCGTG GCCAGAGCAT GGTGTCCCCT ACCACGCCAC GGGGCTGCTG GCCTTCATCC 
     4201 GGCGTGTGAA GGCTTCCACT CCACCTGATG CCGGGCCCAT TGTCATTCAC TGCAGTGCAG 
     4261 GAACTGGCCG CACAGGCTGC TACATCGTCC TGGATGTGAT GCTGGACATG GCTGAATGTG 
     4321 AGGGGGTCGT GGACATTTAC AACTGTGTGA AGACCCTCTG TTCCCGACGG GTCAACATGA 
     4381 TCCAGACGGA GGAACAATAT ATCTTCATCC ACGATGCAAT CTTGGAGGCC TGCCTGTGTG 
     4441 GGGAGACCAC CATCCCTGTC AACGAGTTCA AGGCCACCTA CAGGGAGATG ATCCGCATTG 
     4501 ACCCTCAGAG CAATTCCTCC CAGCTTCGGG AAGAGTTCCA GACGCTGAAC TCGGTCACGC 
     4561 CGCCGCTGGA TGTGGAGGAG TGTAGCATTG CCCTGCTGCC CCGGAATCGA GACAAGAACC 
     4621 GTAGCATGGA TGTGCTGCCA CCAGACCGCT GCCTGCCCTT CCTCATCTCC AGTGATGGGG 
     4681 ACCCCAATAA CTACATCAAT GCAGCACTGA CTGACAGCTA CACACGGAGC GCCGCCTTCA 
     4741 TCGTGACCCT GCACCCGCTG CAGAGTACCA CGCCCGACTT CTGGCGGCTG GTCTACGACT 
     4801 ACGGGTGCAC CTCCATCGTC ATGCTGAACC AACTTAACCA GTCCAACTCC GCCTGGCCCT 
     4861 GCTTGCAGTA CTGGCCGGAG CCAGGCCGAC AGCAGTATGG GCTCATGGAG GTGGAGTTTG 
     4921 TGTCTGGCAC AGCAAACGAG GATTTGGTGT CCCGAGTGTT CCGGGTGCAG AACTCTTCTC 
     4981 GGCTGCAGGA GGGTCACCTG CTGGTACGGC ACTTCCAGTT TCTGCGTTGG TCTGCTTATC 
     5041 GGGACACGCC TGACTCCAGG AAGGCCTTTC TGCACCTGTT GGCTGAGGTG GACAAGTGGC 
     5101 AGGCAGAGAG TGGGGATGGG CGCACCGTGG TGCATTGTCT CAACGGGGGT GGCCGCAGTG 
     5161 GCACCTTCTG CGCCTGTGCC ACGGTCTTGG AGATGATCCG CTGTCACAGC CTGGTGGATG 
     5221 TTTTCTTTGC TGCCAAAACA CTTCGGAACT ACAAGCCCAA TATGGTGGAG ACCATGGATC 
     5281 AGTATCATTT CTGCTACGAC GTGGCCCTGG AGTACCTGGA GGCTCTGGAG TTGAGAACGC 
     5341 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5401 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5461 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5521 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5581 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5641 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5701 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5761 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5821 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5881 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5941 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     6001 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     6061 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     6121 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     6181 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     6241 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6301 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6361 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6421 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6481 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6541 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6601 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6661 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6721 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6781 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6841 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6901 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6961 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     7021 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     7081 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     7141 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     7201 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     7261 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7321 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7381 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7441 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7501 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7561 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7621 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7681 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7741 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7801 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7861 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7921 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7981 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     8041 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     8101 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     8161 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     8221 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     8281 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8341 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8401 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8461 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8521 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8581 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8641 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8701 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8761 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8821 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8881 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8941 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     9001 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     9061 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     9121 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     9181 GCGAATTTT
//