LOCUS       MR223543                7593 bp    DNA     circular  SYN 
DEFINITION  Ano5 (Myc-DDK-tagged) - Mouse anoctamin 5 (Ano5)
ACCESSION   NM_177694
VERSION     NM_177694.6
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3750..3841 
                     /label="Myc-DDK"  
     misc_feature    3871..4456 
                     /label="PolyA signal"  
     misc_feature    3928..3947 
                     /label="Restriction Enzyme"  
     misc_feature    4732..5404 
                     /label="ColE1"  
     misc_feature    5834..6628 
                     /label="Kan/Neo"  
     misc_feature    6663..6965 
                     /label="SV40 ori"  
     misc_feature    7051..7528 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3732..3738 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3732 
                     /label="Ano5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGAGCAG GAAGGCTTAA CAGCCAAAGA AATAGACTAT GCTTTCCAAC 
     1081 AGAATGAGAA CCTGGGCTCC AAAGAGACCA GCTTTCTCAT CCCTGAAGAC TTACAGTCAC 
     1141 CGCCTGAGAA GCGATTCAAT TTGTTCCTGA GGAGGCGTCT TATGTTCCAG AGAAGTGAGC 
     1201 ACAGCAAAGA CTCGGTCTTC TTCAGAGATG GAATCCGGCA GATCGACTTT GTCCTGTCCT 
     1261 ACGTGGAGGA TTTGAAGAAA GATGGAGAGC TGAAGGCAGA AAGAAGACGA GAGTTTGAAC 
     1321 AAAATCTCAG AAAAACAGGT CTTGACTTGG AAACAGAAGA CAAATTGAAC TCTGAAGATG 
     1381 GGAAAACCTA TTTTGTGAAG ATTCACGCCC CATGGGAAGT CCTGGTCACT TACGCTGAAG 
     1441 TGCTGGGAAT CAAGATGCCT ATTAAGTTGA GTGACATCCC ACGGCCAAAG TACCCACCCC 
     1501 TGTCTTACAT GCTTGGGGCG GTGAAGCTTC CATCCTCTGT GAAGTATCCT ACTCCTGAGT 
     1561 ATTTCACTGC CCAGTTCAGC AGACACCGTC AAGAACTCTT CCTCATTGAA GATGAGGCCA 
     1621 CTTTCTTTCC ATCCTCAACA AGGAACAGAA TTGTTTACTA TATTCTGTCA CGATGCCCTT 
     1681 TTGGAGTGGA AGAGGGGAAG AAAAAGATTG GGATCGAGAG ACTGCTGAAC TCGAACACTT 
     1741 ATTTATCTGC CTACCCGCTC CATGATGGCC AGTATTGGAA ACCATCAAAA ACTACTCGCC 
     1801 CCAATGAAAG GTACAACCTT TGCAAGAACT GGGCTCGATT TTCCTACTTT TACAAGGAGC 
     1861 AACCTTTTCA TTTGATTCGG AATTATTTTG GAGAAAAAAT CGGTATCTAT TTTGTATTTC 
     1921 TCGGGTATTA CACAGAAATG CTGTTGTTTG CGGCTCTGGT CGGCTTAGCC TGCTTCATCT 
     1981 ATGGCTTGTT ATCAATGGAA AATAACCGAA CTAGCACTGA AATCTGTGAC CCTGACATCG 
     2041 GAGGTCAGAT GATCATGTGC CCACTTTGCG ATGAAGTGTG TGATTACTGG AGACTGAACA 
     2101 CCACGTGTTT GCATTCAAAG TTCTCCCATC TGTTTGATAA CGAGTCGACT GTGTTCTTTG 
     2161 CGCTCTTCAT GGGGATCTGG GTCACATTGT TTCTGGAGTT TTGGAAGCAG CGACAAGCCA 
     2221 GGCTTGAGTA CGAATGGGAT CTGGTGGACT TTGAAGAAGA ACAGCAGCAG CTCCAGCTTC 
     2281 GGCCTGAATT TGAAGCTATG TGTAAACACA AGAAGATGAA TCCTGTGACA AAGGAAATGG 
     2341 AGCCACACAT GCCTCTATGC CATCGCATCC CATGGTACTT TGTGTCAGGG ACCACAGTGA 
     2401 CCTTTGGGAT GGCTCTCCTC CTCAGCAGCA TGGTGTCCAT CCTCATATAC CGCCTGTCAG 
     2461 TCTTTGCCAC TTTTGCCAGC TTCATGGAAA GCGAAGCCAC CCTGCAGAGC GTGAAGAGCT 
     2521 TCTTTACTCC ACAGCTGGCT ACGGCTCTCT CTGGATCATG CCTGAACTGC ATTGTCATTT 
     2581 TGATACTGAA TTTTTTTTAT GAGAAGATAT CTGCCTGGAT TACGAAAATG GAAATACCAC 
     2641 GAACTCACCA GGAGTATGAG AGCAGCCTCA CTCTGAAGAT GTTTCTCTTC CAATTTGTGA 
     2701 ACTATTACTC ATCTTGCTTC TATGTGGCCT TCTTTAAAGG GAAGTTCGTG GGATATCCTG 
     2761 GGAGTTACAC ATATATGTTC AACATATGGA GAAGCGAAGA GTGTGGTCCT GCAGGCTGTC 
     2821 TGATAGAGCT GACCACCCAG CTAACCATCA TTATGATTGG AAAACAGATT TTTGGAAACA 
     2881 TCCATGAAGC CTTTCAACCC TTGATTTTTA ATTGGTGGAG ACGCCGAAGA GCCCGAACCC 
     2941 ACTCTGAGAA ACTGTACAGT CGTTGGGAGC AAGATCATGA CCTTCAGGTT TATGGACATC 
     3001 GTGGGCTGTT TTATGAGTAC TTGGAAACAG TTATCCAGTT TGGGTTTGCC ACGCTGTTTG 
     3061 TGGCCTCTTT TCCTCTGGCA CCCCTGTTTG CTCTCATGAA CAACATCATG GGAATCCGAG 
     3121 TGGATGCCTG GAAGCTGACA ACTCAGTACA GGAGACCTGT CGCAGCGAAA GCCCACTCCA 
     3181 TAGGTGTCTG GCAAGACATT CTTTTTGGAA TGGCCATCGT TTCTGTTGCA ACTAATGCCT 
     3241 TCATTGTCTC ATTTACATCT GACATCATCC CCCGGTTAGT TTACTTCTAT GCCTACTCAA 
     3301 CGAATTCCAC AGAGCCCTTG AGTGGATACG TCAATAACAG CCTGTCAGTG TTCCTGATAG 
     3361 CTGACTTTCC CAACCACACA GTGCCCATGG AGAAAAAAGA CTTCGTCACC TGCAGGTACA 
     3421 GAGATTATAG GTACCCTCCT GATCATGAGG ATAAGTATAG CCATAACATG CAGTTCTGGC 
     3481 ATGTTCTTGC TGCCAAGATG ACCTTCATCA TAGTGATGGA ACACGTCGTG TTTCTGTTTA 
     3541 AATTTTTATT AGCCTGGCTG ATACCAGATG TTCCTAAAGA TGTTGTGGAG AAAATCAAAC 
     3601 GAGAAAAGTT AATGACTATC AAAATTATTC ATGACTTTGA GCTCAACAAG TTGAAAGAAA 
     3661 ACTTGGATGT TGAATATGGT AACATCATGA AGAATGTTTT AGTAGATGAA GACAATTCCC 
     3721 TGAAGGCTAA AACTACAGTC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3781 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3841 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3901 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3961 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4021 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4081 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4141 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4201 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4261 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4321 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4381 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4441 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4501 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4561 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4621 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4681 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4741 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4801 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4861 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4921 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4981 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5041 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5101 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5161 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5221 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5281 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5341 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5401 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5461 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5521 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5581 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5641 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5701 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5761 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5821 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5881 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5941 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6001 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6061 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6121 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6181 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6241 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6301 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6361 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6421 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6481 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6541 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6601 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6661 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6721 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6781 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6841 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6901 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6961 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7021 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7081 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7141 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7201 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7261 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7321 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7381 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7441 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7501 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7561 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//