LOCUS       MR223515                8805 bp    DNA     circular  SYN 
DEFINITION  Mink1 (Myc-DDK-tagged) - Mouse misshapen-like kinase 1 (zebrafish) (Mink1), transcript variant 2
ACCESSION   NM_176893
VERSION     NM_176893.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4962..5053 
                     /label="Myc-DDK"  
     misc_feature    5083..5668 
                     /label="PolyA signal"  
     misc_feature    5140..5159 
                     /label="Restriction Enzyme"  
     misc_feature    5944..6616 
                     /label="ColE1"  
     misc_feature    7046..7840 
                     /label="Kan/Neo"  
     misc_feature    7875..8177 
                     /label="SV40 ori"  
     misc_feature    8263..8740 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4944..4950 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4944 
                     /label="Mink1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACCCA GCCCCCGCCC GCAGCCTGGA CGACATCGAC CTGTCTGCCC 
     1081 TGCGGGACCC TGCAGGAATC TTTGAGCTGG TGGAGGTGGT TGGCAATGGA ACCTATGGAC 
     1141 AGGTATACAA GGGGCGGCAC GTCAAGACTG GGCAGCTGGC TGCCATTAAG GTCATGGATG 
     1201 TCACAGAGGA TGAGGAGGAA GAGATCAAAC AGGAAATCAA CATGTTAAAG AAGTACTCTC 
     1261 ACCATCGCAA TATTGCCACC TACTATGGGG CCTTTATCAA GAAGAGCCCT CCTGGGAACG 
     1321 ATGACCAGCT CTGGCTGGTG ATGGAGTTCT GCGGTGCTGG TTCAGTGACC GACCTGGTAA 
     1381 AGAACACAAA AGGGAACGCA CTGAAGGAGG ATTGCATTGC TTACATCTGC AGGGAGATTC 
     1441 TCAGGGGTCT TGCCCATCTC CATGCCCACA AGGTGATCCA CAGAGATATC AAGGGACAAA 
     1501 ATGTGCTGCT GACAGAGAAT GCTGAAGTCA AGCTAGTGGA TTTTGGGGTG AGTGCTCAGC 
     1561 TGGACCGCAC TGTGGGCAGG CGGAACACTT TCATTGGAAC CCCATACTGG ATGGCTCCAG 
     1621 AAGTCATTGC CTGTGACGAG AACCCCGATG CCACCTATGA CTACAGGAGT GACATTTGGT 
     1681 CTCTAGGAAT CACAGCCATT GAAATGGCAG AGGGAGCCCC CCCTCTGTGT GACATGCACC 
     1741 CTATGCGGGC CCTCTTCCTC ATCCCTCGGA ACCCTCCCCC CAGGCTCAAG TCAAAGAAAT 
     1801 GGTCTAAGAA GTTCACTGAC TTCATCGACA CGTGTCTCAT CAAGACTTAC CTGAGCCGCC 
     1861 CACCCACCGA ACAGTTACTC AAATTCCCCT TCATCCGAGA CCAGCCCACG GAGCGGCAGG 
     1921 TCCGCATCCA GCTCAAGGAC CACATCGACC GCTCGCGGAA GAAGCGGGGT GAGAAAGAGG 
     1981 AGACAGAGTA TGAGTACAGC GGCAGTGAGG AGGAAGACGA CAGCCATGGA GAGGAAGGCG 
     2041 AGCCAAGCTC CATCATGAAT GTGCCCGGTG AGTCCACACT GCGCAGAGAA TTCCTCAGAC 
     2101 TCCAGCAGGA GAATAAGAGC AACTCTGAGG CTTTAAAGCA GCAGCAGCAG CTGCAGCAAC 
     2161 AGCAGCAGCG GGACCCGGAG GCACACATCA AACACCTGCT GCACCAGCGG CAGCGTCGCA 
     2221 TAGAGGAGCA GAAGGAGGAG CGGCGACGTG TGGAGGAGCA ACAGCGGCGA GAGCGAGAAC 
     2281 AGCGTAAGCT ACAAGAGAAG GAGCAGCAGC GGCGATTGGA AGACATGCAA GCCCTACGAC 
     2341 GAGAGGAAGA GAGGCGGCAA GCAGAGCGGG AACAGGAATA CAAGCGGAAG CAGCTGGAGG 
     2401 AGCAGCGGCA GTCAGAGCGG CTGCAGAGAC AGCTGCAGCA GGAGCACGCC TACCTCAAGT 
     2461 CCCTGCAGCA GCAGCAGCAG CAGCAGCAGC TCCAGAAGCA GCAGCAGCAG CAGCAGCAGA 
     2521 TCCTGCCTGG AGACAGGAAG CCCCTGTATC ATTACGGTCG GGGCATTAAT CCTGCTGACA 
     2581 AGCCAGCATG GGCCCGCGAG GTGGAAGAGA GAGCACGGAT GAACAAGCAG CAGAACTCTC 
     2641 CCTTGGCGAA GGCGAAGCCA AGCAGTGCGG GGCCAGAGCC CCCCATCTCC CAGGCCTCTC 
     2701 CTAGCCCCCC AGGACCTCTT TCCCAGACTC CTCCTATGCA GAGGCCTGTG GAGCCCCAGG 
     2761 AAGGACCGCA CAAGAGCCTG GTGGCACACC GGGTCCCACT GAAGCCATAT GCAGCACCTG 
     2821 TACCCCGATC CCAGTCCCTG CAGGACCAGC CGACTCGAAA CCTGGCTGCC TTCCCAGCCT 
     2881 CCCACGACCC TGACCCTGCT GCTGTCCCTA CACCCACTGC CACACCCAGT GCCCGAGGAG 
     2941 CTGTCATCCG CCAGAATTCA GACCCCACCT CTGAAGGGCC AGGGCCTAGC CCAAACCCTC 
     3001 CATCCTGGGT TCGGCCTGAT AATGAGGCTC CACCTAAGGT TCCACAGAGG ACCTCTTCTA 
     3061 TCGCCACTGC CCTTAACACC AGTGGGGCCG GAGGGTCCCG GCCAGCTCAG GCTGTCCGTG 
     3121 CCAGTAACCC TGACCTCAGG AGGAGTGACC CTGGCTGGGA GCGCTCAGAC AGTGTCCTCC 
     3181 CGGCCTCCCA CGGCCACCTC CCTCAGGCTG GCTCCTTGGA GCGGAACCGA AACCGTGTGG 
     3241 GAGCCTCCAC AAAACTGGAT AGCTCTCCAG TGCTCTCCCC TGGGAACAAA GCCAAGCCTG 
     3301 AAGACCACCG CTCAAGGCCA GGCCGGCCCG CAAGCTATAA GCGAGCAATT GGCGAGGACT 
     3361 TTGTGTTGCT CAAAGAGCGG ACTCTGGATG AGGCCCCTAA GCCTCCCAAG AAGGCCATGG 
     3421 ACTACTCCTC ATCCAGTGAG GAGGTGGAAA GCAGTGAAGA GGAGGAGGAG GAAGGCGATG 
     3481 GGGAGCCGTC AGAGGGGAGC AGAGACACTC CCGGGGGCCG CAGTGATGGT GATACAGACA 
     3541 GCGTCAGCAC CATGGTGGTT CATGATGTTG AGGAGATATC CGGGACCCAG CCCTCATATG 
     3601 GCGGCGGCAC CATGGTGGTC CAGCGTACTC CTGAAGAGGA ACGAAGCCTG CTGCTTGCTG 
     3661 ATAGCAATGG CTACACAAAC CTGCCTGATG TGGTCCAGCC CAGCCACTCA CCTACTGAGA 
     3721 ACAGCAAAGG TCAAAGCCCT CCAACAAAGG ATGGAGGCAG TGATTACCAG TCTCGTGGGC 
     3781 TGGTAAAGGC CCCAGGAAAG AGCTCATTCA CCATGTTTGT GGATCTAGGG ATCTACCAGC 
     3841 CTGGAGGCAG TGGGGACACC ATCCCTATCA CAGCCCTAGT GGGTGGAGAA GGTGGTCGCC 
     3901 TTGATCAACT GCAGTTCGAT GTGAGGAAGG GCTCTGTGGT CAACGTCAAT CCCACCAACA 
     3961 CCCGAGCTCA TAGTGAAACT CCTGAAATTC GCAAGTACAA GAAGCGATTC AACTCAGAGA 
     4021 TCCTATGTGC AGCTCTCTGG GGGGTCAACC TCCTAGTGGG CACAGAGAAT GGGCTGATGT 
     4081 TGCTGGACCG AAGTGGGCAG GGCAAGGTGT ATGGACTTAT TGGGCGACGA CGCTTCCAGC 
     4141 AAATGGATGT CTTAGAAGGG CTCAACTTGC TCATCACCAT CTCAGGGAAA AGGAACAAAC 
     4201 TGCGGGTATA TTACCTGTCC TGGCTTCGGA ACAAGATCCT ACACAATGAC CCAGAGGTGG 
     4261 AAAAGAAGCA GGGGTGGACC ACCGTGGGGG ACATGGAGGG CTGCGGCCAC TACCGTGTTG 
     4321 TGAAATATGA ACGGATTAAG TTCCTGGTCA TTGCCCTGAA GAACTCCGTG GAGGTTTATG 
     4381 CCTGGGCTCC CAAACCCTAC CACAAATTCA TGGCCTTCAA GTCCTTTGCT GACCTCCCTC 
     4441 ACCGCCCTCT ACTGGTGGAC CTGACAGTAG AGGAGGGACA GCGGCTCAAG GTCATCTATG 
     4501 GCTCCAGTGC TGGCTTCCAT GCTGTGGATG TTGATTCTGG GAACAGCTAT GACATCTACA 
     4561 TCCCTGTACA TATCCAGAGC CAGATCACAC CCCACGCCAT CATCTTCCTC CCCAACACTG 
     4621 ATGGCATGGA GATGCTGCTG TGCTATGAAG ATGAGGGTGT CTATGTCAAC ACTTACGGGC 
     4681 GGATCATCAA GGATGTGGTG CTGCAGTGGG GAGAGATGCC CACCTCTGTG GCCTACATCT 
     4741 GCTCCAACCA GATAATGGGC TGGGGTGAGA AGGCCATAGA GATCCGCTCT GTGGAGACAG 
     4801 GCCACCTAGA TGGGGTCTTC ATGCACAAAC GAGCCCAGAG GCTCAAGTTC CTGTGTGAGC 
     4861 GCAATGACAA GGTGTTTTTT GCCTCTGTCC GCTCTGGAGG AAGCAGCCAA GTTTACTTTA 
     4921 TGACTCTGAA CCGTAACTGC ATCATGAACT GGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4981 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5041 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5101 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5161 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5221 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5281 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5341 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5401 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5461 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5521 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5581 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5641 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5701 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5761 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5821 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5881 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5941 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6001 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6061 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6121 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6181 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6241 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6301 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6361 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6421 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6481 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6541 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6601 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6661 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6721 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6781 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6841 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6901 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6961 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7021 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7081 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7141 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7201 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7261 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7321 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7381 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7441 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7501 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7561 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7621 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7681 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7741 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7801 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7861 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7921 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7981 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8041 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8221 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8281 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8341 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8401 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8461 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8521 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8581 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8641 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8701 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8761 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//