LOCUS       MR223498                8478 bp    DNA     circular  SYN 
DEFINITION  Grm1 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 1 (Grm1), transcript variant 1
ACCESSION   NM_016976
VERSION     NM_016976.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4635..4726 
                     /label="Myc-DDK"  
     misc_feature    4756..5341 
                     /label="PolyA signal"  
     misc_feature    4813..4832 
                     /label="Restriction Enzyme"  
     misc_feature    5617..6289 
                     /label="ColE1"  
     misc_feature    6719..7513 
                     /label="Kan/Neo"  
     misc_feature    7548..7850 
                     /label="SV40 ori"  
     misc_feature    7936..8413 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4617..4623 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4617 
                     /label="Grm1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCGGCTC CTCTTGATTT TCTTCCCAAT GATCTTTTTG GAGATGTCCA 
     1081 TTTTGCCCAG GATGCCTGAC AGAAAAGTAC TGCTGGCAGG TGCCTCGTCC CAGCGCTCTG 
     1141 TGGCGAGAAT GGACGGAGAT GTCATCATCG GAGCCCTCTT CTCAGTCCAT CACCAGCCTC 
     1201 CAGCCGAAAA GGTACCGGAA AGGAAGTGTG GGGAGATCAG AGAACAGTAT GGTATCCAGA 
     1261 GAGTGGAGGC CATGTTCCAC ACATTAGATA AGATTAATGC GGACCCGGTG CTCCTGCCCA 
     1321 ACATCACTCT GGGCAGTGAG ATCCGGGACT CCTGCTGGCA CTCTTCTGTG GCTCTGGAAC 
     1381 AGAGCATTGA GTTCATCAGA GACTCCCTGA TTTCCATCCG AGATGAGAAG GATGGACTGA 
     1441 ACCGCTGCCT GCCTGATGGC CAGACCCTAC CCCCAGGCAG GACTAAGAAG CCTATTGCTG 
     1501 GAGTGATCGG CCCTGGCTCC AGCTCTGTGG CCATTCAAGT CCAGAATCTT CTGCAGCTGT 
     1561 TCGACATCCC ACAAATCGCC TATTCTGCCA CGAGCATAGA CCTGAGCGAC AAAACTTTGT 
     1621 ACAAATACTT CCTGAGGGTG GTCCCTTCTG ACACTTTGCA GGCAAGGGCG ATGCTTGATA 
     1681 TCGTCAAGCG GTACAACTGG ACCTATGTCT CTGCAGTCCA CACAGAAGGG AATTACGGCG 
     1741 AGAGTGGAAT GGATGCTTTC AAAGAGTTGG CTGCCCAGGA AGGACTCTGC ATCGCACACT 
     1801 CGGACAAAAT CTACAGCAAT GCTGGCGAGA AGAGCTTTGA TCGGCTTCTG CGCAAGCTCC 
     1861 GAGAGCGGCT TCCCAAGGCC AGGGTTGTGG TCTGCTTCTG CGAGGGCATG ACAGTGCGGG 
     1921 GGTTACTGAG TGCCATGCGC CGCCTTGGCG TCGTGGGAGA GTTCTCACTC ATTGGAAGTG 
     1981 ATGGATGGGC AGACAGAGAT GAAGTCATCG AAGGCTATGA GGTGGAAGCC AATGGGGGAA 
     2041 TCACAATAAA GCTGCAGTCT CCAGAGGTCA GGTCGTTTGA TGACTACTTT CTGAAGCTGA 
     2101 GGCTGGACAC CAACACGAGG AATCCTTGGT TCCCTGAGTT CTGGCAACAT CGCTTCCAGT 
     2161 GTCGCCTACC TGGACACCTC TTGGAAAACC CCAACTTTAA AAAAGTCTGC ACAGGAAATG 
     2221 AAAGCTTGGA AGAAAATTAT GTCCAGGACA GCAAAATGGG ATTTGTCATC AACGCCATCT 
     2281 ATGCCATGGC ACACGGCCTA CAGAACATGC ACCATGCTCT ATGTCCTGGC TACGTGGGCC 
     2341 TTTGTGATGC CATGAAGCCC ATTGACGGCA GGAAGCTCCT GGATTTCCTC ATCAAATCCT 
     2401 CTTTTGTTGG AGTGTCTGGA GAGGAGGTCT GGTTCGATGA GAAGGGGGAT GCACCTGGAA 
     2461 GGTATGACAT TATGAATCTG CAGTACACAG AGGCTAATCG CTATGACTAT GTCCATGTGG 
     2521 GAACCTGGCA TGAAGGTGTG CTGAATATCG ATGATTACAA AATCCAGATG AACAAAAGCG 
     2581 GAATGGTACG ATCTGTGTGC AGCGAGCCTT GCTTAAAGGG TCAGATTAAG GTCATACGGA 
     2641 AAGGGGAAGT GAGCTGCTGC TGGATCTGCA CAGCCTGCAA AGAGAATGAG TTTGTGCAAG 
     2701 ATGAGTTTAC CTGCAGAGCC TGTGACCTGG GGTGGTGGCC CAATGCAGAG CTCACAGGCT 
     2761 GTGAGCCCAT TACTATCCGT TACCTCGAGT GGAGTGACAT AGAATCCATC ATAGCCATCG 
     2821 CCTTTTCTTG CCTGGGCATC CTCGTGACGC TATTTGTCAC CCTCATCTTT GTGCTGTACC 
     2881 GGGACACACC TGTGGTCAAA TCCTCCAGTA GAGAGCTCTG CTATATCATT CTGGCTGGTA 
     2941 TTTTCCTCGG CTACGTATGC CCTTTCACCC TCATCGCCAA ACCTACTACC ACATCCTGCT 
     3001 ACCTCCAGCG CCTCCTAGTT GGCCTCTCTT CTGCCATGTG CTACTCTGCT CTTGTGACCA 
     3061 AAACCAATCG TATTGCACGC ATCCTGGCTG GCAGCAAGAA GAAGATCTGT ACCCGGAAGC 
     3121 CCAGGTTCAT GAGCGCTTGG GCCCAAGTGA TCATAGCCTC CATTCTGATT AGTGTACAGC 
     3181 TGACACTAGT GGTGACCTTG ATCATCATGG AGCCTCCCAT GCCCATTTTG TCCTACCCGA 
     3241 GCATCAAGGA AGTCTATCTT ATCTGCAATA CCAGCAACCT GGGTGTAGTG GCACCTGTGG 
     3301 GTTACAACGG ACTTCTCATC ATGAGCTGTA CCTACTATGC CTTCAAGACC CGCAACGTGC 
     3361 CGGCCAATTT CAATGAGGCT AAATACATCG CCTTCACTAT GTACACCACC TGCATCATCT 
     3421 GGCTGGCTTT TGTTCCCATT TACTTTGGGA GCAACTACAA GATTATCACT ACCTGCTTCG 
     3481 CAGTGAGCCT CAGTGTGACG GTGGCCCTGG GCTGCATGTT CACTCCCAAG ATGTACATCA 
     3541 TTATTGCCAA ACCCGAGAGG AATGTCCGCA GTGCCTTCAC CACCTCTGAT GTAGTGCGCA 
     3601 TGCACGTCGG TGACGGCAAG CTGCCATGCC GCTCCAACAC CTTCCTCAAC ATTTTCCGGA 
     3661 GAAAGAAGCC TGGGGCAGGG AACGCCAATT CTAACGGCAA GTCTGTGTCA TGGTCTGAAC 
     3721 CAGGTGGAAG ACAGGCGCCC AAGGGACAGC ATGTGTGGCA GCGCCTCTCT GTGCACGTGA 
     3781 AGACCAATGA GACGGCCTGT AACCAAACAG CCGTAATTAA ACCCCTCACT AAAAGTTACC 
     3841 AAGGCTCTGG CAAGAGTCTG ACCTTTTCAG ACGCCAGCAC CAAGACCCTT TACAACGTGG 
     3901 AGGAAGAGGA CAATACCCCT TCAACTCACT TCAGCCCTCC CAGCAGCCCT TCCATGGTGG 
     3961 TGCACCGACG CGGGCCACCC GTGGCCACCA CGCCACCTCT GCCACCCCAC CTTAGCGCAG 
     4021 AAGAGACGCC CTTGTTCCTG GCTGATTCTG TCATCCCCAA GGGCTTGCCT CCTCCTCTCC 
     4081 CGCAGCAGCA ACAGCAGCCG CCCCCTCAGC CGCCTCCGCA GCAGCCCAAA TCCCTGATGG 
     4141 ACCAGCTGCA AGGCGTGGTC ACCAACTTTG GCTCTGGGAT TCCAGACTTC CACGCGGTGC 
     4201 TGGCAGGCCC GGGGACACCA GGGAACGGGC TGCGCTCCCT GTACCCGCCC CCGCCTCCAC 
     4261 CGCAACACCT GCAGATGTTG CCCCTGCAGC TGAGCACCTT CCGAGAGGAG CCCATCTCCC 
     4321 CTCCGGGGGA GGACGACGAT GATGACAGCA GCGAGAGGTT CAAGCTCCTG CAGGAGTTCG 
     4381 TGTATGAGCG TGAAGGGAAC ACGGAGGAAG ACGATCTGGA AGAGGAGGAG GACCTGCCTG 
     4441 CAGCCAGCAA GCTGACGCCT GAGGATTCGC CTGCCCTGAC GCCCCCTTCT CCTTTCCGAG 
     4501 ATTCCGTGGC CTCTGGCAGC TCAGTGCCCA GTTCCCCAGT GTCTGAGTCA GTGCTCTGCA 
     4561 CCCCTCCAAA TGTGACCTAT GCCTCTGTCA TTCTGAGGGA CTACAAGCAA AGCTCTTCCA 
     4621 CTCTGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4681 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4741 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4801 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4861 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4921 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4981 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5041 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5101 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5161 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5221 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5281 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5341 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5401 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5461 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5521 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5581 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5641 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5701 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5761 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5821 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5881 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5941 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6001 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6061 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6121 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6181 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6241 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6301 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6361 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6421 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6481 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6541 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6601 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6661 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6721 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6781 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6841 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6901 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6961 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7021 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7081 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7141 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7201 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7261 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7321 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7381 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7441 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7501 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7561 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7621 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7681 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7741 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7801 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7861 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7921 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7981 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8041 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8101 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8161 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8221 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8281 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8341 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8401 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8461 AAATTTAACG CGAATTTT
//