LOCUS       MR223442                9255 bp    DNA     circular  SYN 
DEFINITION  Nfat5 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant a
ACCESSION   NM_133957
VERSION     NM_133957.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5412..5503 
                     /label="Myc-DDK"  
     misc_feature    5533..6118 
                     /label="PolyA signal"  
     misc_feature    5590..5609 
                     /label="Restriction Enzyme"  
     misc_feature    6394..7066 
                     /label="ColE1"  
     misc_feature    7496..8290 
                     /label="Kan/Neo"  
     misc_feature    8325..8627 
                     /label="SV40 ori"  
     misc_feature    8713..9190 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5394..5400 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5394 
                     /label="Nfat5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGGTGCT TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT 
     1081 CTACCTCAGT CACCGACAGC AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG 
     1141 TAAGTAACAG AGGGGTAAGT GAAAAGCAGT TAACCGGTAA CACAGTTCAG CAGCATCCAT 
     1201 CAACCCCGAA GAGGCACACA GTTTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA 
     1261 ACAGTCGGAT GTCCTGCCAG GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA 
     1321 TGTGGATGGA GGATTCCCCC TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA 
     1381 ACACTGAGGT ACCTCGTAAA TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC 
     1441 GACGAGATTG TGAAGAATCT AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC 
     1501 ACTATGTGCT TTCTCAGCTT ACCACGGACA ACAAAGGCAA CTCAAAAGCT GGAAATGGAA 
     1561 CATTGGACAG CCAAAAGGGA ACTGGAGTAA AGAAGAGCCC TATGTTGTGC GGACAGTATC 
     1621 CGGTTAAAAG TGAGGGGAAG GAGCTGAAGA TAGTGGTACA GCCTGAAACC CAACACCGAG 
     1681 CCCGGTACCT GACAGAGGGC AGCCGAGGCT CTGTAAAAGA CAGAACACAG CAAGGCTTTC 
     1741 CTACGGTGAA GCTGGAAGGC CATAATGAAC CAGTGGTGTT GCAGGTATTT GTGGGCAATG 
     1801 ATTCTGGTCG AGTAAAACCA CATGGATTCT ATCAGGCCTG TAGAGTAACT GGGCGAAATA 
     1861 CAACTCCCTG CAAAGAAGTG GACATTGAAG GTACCACTGT TATAGAAGTT GGTCTTGATC 
     1921 CTAGCAACAA CATGACACTG GCGGTGGACT GTGTTGGAAT ATTGAAGCTG AGGAATGCCG 
     1981 ATGTTGAAGC CAGAATTGGA ATTGCTGGAT CTAAGAAAAA GAGCACTCGT GCCAGATTGG 
     2041 TTTTTCGAGT TAATATCACA AGGAAAGATG GCTCTACTCT GACATTGCAG ACACCTTCTT 
     2101 CCCCCATTTT ATGCACTCAG CCAGCAGGAG TTCCTGAGAT CTTAAAGAAA AGCTTGCATA 
     2161 GCTGTTCAGT GAAAGGAGAG GAAGAAGTAT TTTTAATTGG GAAAAACTTT CTGAAAGGAA 
     2221 CTAAAGTTAT TTTCCAGGAA AATGTTTCTG ATGAAAACTC TTGGAAATCA GAAGCTGAAA 
     2281 TTGACATGGA GTTATTCCAT CAGAACCATC TTATTGTGAA GGTTCCTCCG TATCATGACC 
     2341 AACATATAAC TTTGCCTGTA TCAGTGGGAA TATATGTTGT GACCAATGCT GGAAGATCTC 
     2401 ATGATGTTCA GCCATTTACG TACACTCCGG ATCCAGCAGC TGGTGCTTTG AATGTAAATG 
     2461 TGAAAAAGGA GATATCTAGT CCAGCAAGAC CTTGCTCTTT TGAAGAAGCC ATGAAAGCAA 
     2521 TGAAAACGAC TGGATGTAAC GTAGATAAGG TGACTATCCT TCCTAATGCC CTGATCACTC 
     2581 CACTCATATC AAGCAGTATG ATTAAGACTG AAGATGTTAC TCCAATGGAA GTAACTTCAG 
     2641 AAAAAAGATC TTCCCCAATT TTTCAGACTA CAAAGAGCAT TGGATCAACC CAGCAAACCT 
     2701 TAGAAACTAT TTCTAACATA GCGGGGGGTG CACCCTTCTC CTCACCGTCG TCATCTTCCC 
     2761 ACTTAACTCC TGAAAGTGAG AATCAGCAGC AGCTTCAGCC CAAGGCATAC AACCCAGAGA 
     2821 CCCTGACAAC TATCCAGACA CAGGACATCT CACAGCCCGG GACCTTTCCC GCAGTTTCTG 
     2881 CTGCTAGTCA GCTGCCCAGC AGCGATGCAC TACTGCAGCA GGCAACACAG TTTCAGACAA 
     2941 GAGAAGCTCA GTCCAGAGAC ACAATACAGT CAGATACAGT GGTTAACTTG TCACAGTTAA 
     3001 CTGAAGCATC ACAGCAGCAG CAATCCCCAC TACAAGAACA AGCACAAACG TTACAGCAGC 
     3061 AGATACCATC AAATATTTTT CCATCGCCAA GCAGTGTCAG CCAGCTCCAG AGTACTATTC 
     3121 AGCAGCTGCA AGCAGGAAGT TTCACAGGCA GCGCTGCTGG CGGCAGGAGT GGAAGCGTTG 
     3181 ATTTGGTCCA GCAGGTTTTA GAGGCACAAC AGCAGTTATC GTCTGTTCTG TTTTCTACTC 
     3241 CAGATGGGAA TGAGAATGTT CAAGAACAGC TTAATGCAGA CATTTTCCAA GTCAGTCAAA 
     3301 TTCAAAATAG TGTAAGCCCT GGAATGTTTT CTTCAGCGGA GTCTGCAGTT CACACTAGAC 
     3361 CAGATAACTT ACTACCTGGG AGGGCTGACA GCGTCCATCA ACAGACTGAA AATACACTGT 
     3421 CTAATCAGCA GCAGCAGCAA CAGCAGCAGC AGCAAGTGAT GGAGTCATCA GCTGCAATGG 
     3481 TGATGGAGAT GCAGCAGAGC ATTTGCCAAG CAGCTGCCCA GATCCAGTCA GAGCTGTTTC 
     3541 CTTCAGCTGC TTCAGCAAGT GGAAGCCTTC AGCAGTCTCC AGTTTACCAG CAGCCTTCTC 
     3601 ACATGATGAG TGCACTGCCT ACCAACGAGG ACATGCAGAT GCAGTGTGAG CTGTTCTCGT 
     3661 CTCCCCCTGC AGCTTCTGGA AACGAAACTT CCACAACCAC CACGCCACAG GTGGCAACCC 
     3721 CTGGCTCCAC CATGTTTCAG ACACCAAGTT CAGGAGATGG AGAAGAAACT GGAGCACAAG 
     3781 CAAAACAGAT TCAGAACAGC GTCTTTCAGA CAATGGTCCA AATGCAGCGC AGTGGTGACA 
     3841 GCCAACCTCA AGTAAACCTC TTTTCATCTA CAAAAAATAT AATGAGTGTT CAGAATAATG 
     3901 GTACCCAGCA ACAAGGGAAT AGCTTGTTCC AGCAAGGGAG CGAGATGATG TCACTTCAGT 
     3961 CTGGGAACTT TTTGCAGCAG TCCTCTCATT CACAGGCTCA GCTCTTTCAT CCTCAAAATC 
     4021 CTATTGCTGA TGCTCAGAAC CTTTCCCAGG AAACTCAAGG TTCAATTTTT CATAGTCCAA 
     4081 ATCCTATTGT CCACAGTCAA ACTTCCACAG CATCCTCTGA ACAACTGCAG CCTTCAATGT 
     4141 TTCACTCTCA GAATACCATT GCTGTGTTAC AGGGCTCTTC AGTTCCTCAA GACCAGCAGT 
     4201 CACCCAACAT ATTTCTTTCC CAAAGTTCTA TCAATAATCT TCAAACTAAC ACAGTAGCCC 
     4261 AAGAAGAGCA GATTTCATTT TTTGCAGCAC AGAACTCAAT TTCTCCACTT CAGTCAACAT 
     4321 CAAACACTGA GCAGCAAGCT GCTTTCCAAC AGCAGCCTCC AATCTCACAC ATCCAGACCC 
     4381 CTATTCTTTC CCAGGAACAG GCACAACCCT CTCAGCAAGG GTTATTTCAG CCTCAGGTGG 
     4441 CCCTTGGCTC CCTGCCTCCT AATCCAATGC CTCAAAACCA GCAAGGTCCA ATTTTCCAAA 
     4501 CACAGCGCCC AATAGTTGGC ATGCAGAGTA ACTCTCCATC CCAGGAGCAG CAGCAGCAGC 
     4561 AACAGCAGCA ACAGCAGCAG CAGCAACAGC AACAACAACA GCAACAGAGC ATTTTATTCA 
     4621 GTAATCAGAA TGCCATGGCT ACAATGGCCT CCCAGAAGCA GCCACCACCA AACATGATGT 
     4681 TTAGCCCAAA CCAGAACCCA ATGGCTAGTC AGGAGCAGCA GAACCAGTCA ATTTTTCACC 
     4741 AGCAAAGTAA TATGGCCCCA ATGAACCAAG AGCAGCAGCC CATGCAATTT CAGAATCAGC 
     4801 CTACGGTTTC CTCACTTCAG AACCCAGGTC CTACGCAGTC TGAGTCACCA CAGACCTCCT 
     4861 TGTTCCATAG CTCTCCTCAG ATCCAGTTGG TTCAAGGGTC ACCTAGTTCT CAAGATCAGC 
     4921 AAGTAACACT CTTCCTCTCT CCAGCATCCA TGTCTGCATT GCAAACCAGT ATAAACCAAC 
     4981 CAGACATGCA GCAGTCTCCT CTTTATTCCC CGCAGAACAA CATCCCTGGA ATCCAAGGAA 
     5041 GCACTTCTTC ACCCCAGCCA CAGGCTACTT TATTTCACAA CACTACAGGA GGCACAATAA 
     5101 ACCAAATACA AAATTCTCCT GGCTCATCTC AGCAGACTTC AGGAATGTTC TTATTTGGCA 
     5161 TTCAAAATAA TTGTAGTCAG CTTTTAACCT CCGGACCAGC TACATTGCCA GATCAGCTGA 
     5221 TGGCCATAAA TCAGCAGGGC CAACCACAAA ATGAGGGCCA ATCTTCTGTG ACAACACTTC 
     5281 TTTCTCAGCA AATGCCAGAG ACTTCCCCAC TGGCCTCCTC TGTGAACAGC AGTCAGAACA 
     5341 TGGAAAAGAT TGATTTGCTT GTTTCATTGC AAAGCCAAGG GAACAATTTA ACCGGCTCCT 
     5401 TTACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5461 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5521 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5581 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5641 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5701 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5761 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5821 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5881 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5941 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6001 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6061 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6121 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6181 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6241 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6301 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6361 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6421 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6481 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6541 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6601 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6661 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6721 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6781 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6841 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6901 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6961 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7021 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7081 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7141 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7201 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7261 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7321 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7381 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7441 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7501 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7561 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7621 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7681 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7741 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7801 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7861 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7921 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7981 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8041 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8101 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8161 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8221 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8281 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8341 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8401 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8461 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8521 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8581 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8641 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8701 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8761 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8821 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8881 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8941 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9001 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9061 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9121 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9181 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9241 TTTAACGCGA ATTTT
//