LOCUS       MR223434                7356 bp    DNA     circular  SYN 
DEFINITION  Cdc27 (Myc-DDK-tagged) - Mouse cell division cycle 27 homolog (S. cerevisiae) (Cdc27)
ACCESSION   NM_145436
VERSION     NM_145436.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3513..3604 
                     /label="Myc-DDK"  
     misc_feature    3634..4219 
                     /label="PolyA signal"  
     misc_feature    3691..3710 
                     /label="Restriction Enzyme"  
     misc_feature    4495..5167 
                     /label="ColE1"  
     misc_feature    5597..6391 
                     /label="Kan/Neo"  
     misc_feature    6426..6728 
                     /label="SV40 ori"  
     misc_feature    6814..7291 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3495..3501 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3495 
                     /label="Cdc27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGTGCTG CAGGAACCTG TCCAGGCTGC TATATGGCAA GCGCTAAACC 
     1081 ACTATGCTTA CCGAGATGCA GTTTTCCTCG CAGAACGACT ATATGCAGAA GTACATTCAG 
     1141 AAGAAGCCTT GTTTTTACTG GCAACCTGTT ACTACCGCTC AGGAAAGGCT TATAAAGCAT 
     1201 ATAGACTCTT GAAAGGACAC AGTTGTACCA CCCCACAGTG TAAATACCTG CTTGCAAAAT 
     1261 GTTGTGTTGA CCTCAGCAAG CTTGCAGAAG GGGAACAGAT CTTATCTGGT GGAGTGTTTA 
     1321 ATAAGCAGAA AAGCCATGAC GACCTTGTCA CTGAGTTTGG AGATTCAGCT TGCTTCACTC 
     1381 TTTCCTTGTT GGGACATGTG TATTGCAAGA CAGATCGGCT TGCCAAAGGG TCAGAATGTT 
     1441 ACCAAAAGAG CCTTAGTTTA AATCCTTTCC TCTGGTCTCC CTTTGAATCG TTATGTGAAA 
     1501 TAGGTGAGAA GCCAGATCCT GACCAAACAT TTAAATTAAC ATCTCTACAG AATTTTAGCA 
     1561 GTTGTCTTCC CAACACTTGT ACAACTCTAG TATCTAATCA CAGTTTATCT CACAGACAGC 
     1621 CTGAGACAGT CCTTACAGAA ACACCCCAAG ACACAATTGA ATTAAACAGA CTGAATTTAG 
     1681 AATCTTCCAA TTCAAAGTAC TCCTTGAATA CAGATTCCTC CGTGTCTTAC ATTGATTCAA 
     1741 CTGTAATTTC ACCAGATAAC GTCCCCCTTG GACCTGGTAC TGCCATATTA TCTAAACAGG 
     1801 TTCAAAATAA ACCAAAAACT GGTCGAAGTT TATTAGGAGG ACCAACTGCT CTTAGCCCAT 
     1861 TAACCCCAAG TTTTGGGATT TTGCCATTAG AAACCCCAAG TCCTGGAGAT GGATCCTATT 
     1921 TACAAAACTA CACTAATACA CCTTCTGTAA TTGATGTGGC ACCCACCGGA GCACCTACAA 
     1981 AAAAGTCTGT TGCAAGAATG GGCCAAACTG GAACAAAGTC TGTCTTCTCA CAGAGTGGAA 
     2041 ATAGTCGAGA GGTCACACCA GTACTTGTTG CACAAACACA AAGTTCTGGC CCACAAACAA 
     2101 GTACAACACC TCAGGTATTG AGCCCCACTA TCACATCTCC CCCAAACGCA TTGCCTCGGA 
     2161 GAAGTTCCCG CCTTTTCACT AGTGACAGTT CTACAACCAA GGAGAATAGC AAAAAGTTAA 
     2221 AAATGAAGTT TCCACCTAAA ATCCCTAACA GAAAAACAAA AAGTAAAACT AATAAAGGAG 
     2281 GACTAACTCA GCCCAGCATA AACGATAGTC TGGAAATTAC AAAACTGGAC TCCTCTATCA 
     2341 TTTCAGAAGG GAAAATAACC ACAGTCACAC CTCAGATCCA GGCATTTAAC CTACAAAAGG 
     2401 CAGCAGCAGA AGGTTTGATG AGCCTTCTTC GTGAAATGGG AAAAGGTTAT TTAGCTTTGT 
     2461 GTTCATACAA CTGCAAAGAA GCTATCAATA TTTTGAGCCA TCTACCTTCT CATCACTACA 
     2521 GTACTGGTTG GGTCCTATGC CAGATTGGAC GGGCTTATTT TGAACTTTCA GAATACATGC 
     2581 AGGCTGAAAG AATATTCTCA GAGGTTAGAA GGATTGAGAG TTTCAGAGTT GAAGGAATGG 
     2641 AGATCTACTC TACAACACTC TGGCATCTTC AGAAAGACGT TGCTCTTTCA GTTCTTTCAA 
     2701 AAGATTTAAC AGACATGGAT AAGAATTCAC CAGAGGCCTG GTGTGCTGCA GGGAATTGTT 
     2761 TCAGTCTACA ACGAGAGCAT GATATAGCAA TTAAATTCTT CCAAAGAGCT ATCCAGGTCG 
     2821 ATCCAAATTA CGCTTACGCC TATACTCTAT TAGGACATGA GTTTGTGTTA ACTGAAGAAC 
     2881 TAGATAAAGC ATTAGCATGT TTTCGAAATG CTATAAGAGT AAATCCCAGA CATTACAATG 
     2941 CATGGTATGG TTTAGGAATG ATTTATTACA AGCAAGAGAA GTTCAGCCTC GCAGAAATGC 
     3001 ATTTCCAGAA AGCACTTGAT ATCAACCCTC AGAGTTCAGT TTTACTTTGC CACATTGGAG 
     3061 TAGTTCAGCA TGCACTAAAG AAGTCTGAGA AGGCTTTGGA TACCCTAAAC AAAGCCATTG 
     3121 TTATCGATCC CAAGAACCCT CTATGCAAAT TTCACAGAGC CTCGGTTTTA TTTGCAAATG 
     3181 AAAAATACAA GTCTGCTTTA CAAGAACTCG AGGAACTGAA GCAGATTGTC CCCAAAGAGT 
     3241 CCCTCGTTTA CTTCCTAATA GGAAAGGTTT ACAAGAAGTT GGGTCAAACT CATCTCGCCT 
     3301 TGATGAATTT CTCTTGGGCT ATGGATTTAG ATCCTAAAGG AGCCAATAAT CAGATTAAAG 
     3361 AGGCAATTGA CAAGCGCTAC CTTCCAGATG ATGAGGAGCC AATAACCCAG GAGGAACAGA 
     3421 TCATGGGGAC AGATGAATCC CAGGAGAGCA GCATGACAGA TGCAGATGAC ACACAACTTC 
     3481 ATGCGGCGGA AAGTGACGAA TTTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3541 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3601 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3661 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3721 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3781 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3841 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3901 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3961 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4021 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4081 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4141 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4201 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4261 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4321 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4381 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4441 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4501 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4561 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4621 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4681 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4741 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4801 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4861 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4921 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4981 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5041 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5101 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5161 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5221 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5281 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5341 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5401 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5461 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5521 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5581 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5641 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5701 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5761 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5821 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5881 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5941 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6001 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6061 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6121 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6181 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6241 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6301 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6361 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6421 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6481 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6541 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6601 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6661 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6721 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6781 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6841 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6901 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6961 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7021 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7081 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7141 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7201 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7261 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7321 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//