LOCUS       MR223355                8130 bp    DNA     circular  SYN 
DEFINITION  Ube3c (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3C (Ube3c)
ACCESSION   NM_133907
VERSION     NM_133907.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4287..4378 
                     /label="Myc-DDK"  
     misc_feature    4408..4993 
                     /label="PolyA signal"  
     misc_feature    4465..4484 
                     /label="Restriction Enzyme"  
     misc_feature    5269..5941 
                     /label="ColE1"  
     misc_feature    6371..7165 
                     /label="Kan/Neo"  
     misc_feature    7200..7502 
                     /label="SV40 ori"  
     misc_feature    7588..8065 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4269..4275 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4269 
                     /label="Ube3c"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCAGCTTC GAAGGCGACT TCAAGACAAG GCCCAAAGTG TCTCTCGGTG 
     1081 GCGCGAGCAG GAAGGAAGAA AAGGCTTCTC TTTTACATCG CACTCAGGAA GAAAGACGCA 
     1141 AGAGAGAGGA AGAAAGGCGA AGATTGAAAA ATGCAGTAAT AATCCAATCT TTTATCCGAG 
     1201 GCTATAGAGA CAGAAAACAA CAATACTTCA TCCAAAGAAG CGCGTTTGAC CAATGCACTG 
     1261 ACTCGGCTCA GCCTGGTGGT ACCTTCTGCC TTGCTGATGG TCCCAACCTT ACCCTCTTGG 
     1321 TAAGGCAGCT TCTGTTCTTT TACAAGCAGA GTGAAGACTC CAAACGTTTG ATATGGCTTT 
     1381 ATCAGAACTT AATTAAACAC AGCTCTCTGT TTGTCAAGCA ATTGGATGGA TCAGAGAGAC 
     1441 TGACATGCTT ATTTCAAATA AAGAGGTTGA TGAGCCTCTG TTGCAGGTTG CTGCAAAACT 
     1501 GTAGTGATGA CAGTCTGAAT GTTGCACTTC CAATGCGAAT GCTTGAGGTG TTTACATCTG 
     1561 AGAATACATA CTTGCCTGTT TTACAAGATT CTAGCTATGT GGTGTCAGTG ATTGAACAAA 
     1621 TTTTGCACTA CATGGTTCAC AGTGGATATT ATAGATCTCT CTACTTGTTG ATTAATAGCA 
     1681 AGCTTCCATC AAGTATTGAA TATTCTGATT TATCTCGAGT TCCTATAGCA AAAATTTTGC 
     1741 TGGAGAATGT CCTAAAACCA TTGCACTTTA CGTATAGCTC CTGTCCTGAA GCCTCAAGGC 
     1801 ATCAAGTTTT CTCAGCCTTC ACAGAGGAGT TTCTGGGAGC ACCTTTTACC GATCAAATTT 
     1861 TTCATTTCGT CATTCCGGCC TTTGCAGATG CACAAACTGT GTTCCCTTAT GAGCCCTTTC 
     1921 TCAATGCATT GTTGTTACTT GAGAGTCAAA GTTCAAAACG GTGTAGTGGA GTGCCATGGC 
     1981 TCTTCTATTT TGTTTTAACT GTTGGCGAGA ATTATTTAGG AGCACTCTCT GAGGATGGTC 
     2041 TACTGGTTTA TCTGCGAGTG CTTCAGACCT TCCTCTCTCA GCTACCTGCT TCACCCACTG 
     2101 GTACAGGCTG TCCTGATTCC ACCAGTGACT CTGAGGACGA TAACGAGGAG ACTGACCAAC 
     2161 CCAACAGCCC TGAGGATGGC AGGGTGTCAG CCCCATATAT TACTGAAGAG TGTCTGAGAA 
     2221 AACTGGATAC GAAGCAGCAA ACCAACACCC TGCTGAACCT GGTGTGGAGG GACTCGGCCA 
     2281 GTGAGGAGGT CTTCACAAGG ATGGCTTCTA TCTGCCACAC ATTGATGGTG CAGCACCGAA 
     2341 TGATGGTGCC CAAAGTCAGG CTTCTCTACA GTTTAGCCTT CAATGCCAGG TTTTTGAGGC 
     2401 ATCTTTGGTT TCTCATCTCT TCAATGACAA CACAGATGAT CACAGGATCT ATGGTGCCAT 
     2461 TGCTTCAGTT GATATCGCGG GGTTCGCCCA TGTCTTTTGA AGATTCTAGT CGAATCATCC 
     2521 CACTCTTTTA CCTCTTCAGC TCCTTGTTCA GTCATTCGCT GATCTCCATA CATGACAATG 
     2581 AATTTTTTGG TGACCCCATA GAAGTTGTAG GTCAGAGACA ATCATCCATG ATGCCTTTCA 
     2641 CTCTGGAGGA GTTAATCCTG CTGTCACGAT GCCTTCGAGA TGCCTGCTTG GGGATCATCA 
     2701 AGCTAGCATA CCCGGAAACA AAGCCAGAAG TTCGGGAAGA ATATGTCACC GCATTTCAGA 
     2761 GTATTGGGGT CACTACTAAT TCTGAGATGC AGCAGTGCAT ACAAATGGAA CAGAAGCGAT 
     2821 GGGTTCAGCT GTTCAAGGTT ATCACCAACC TAGTGAAAAT GCTGAAGTCT AGAGACACGA 
     2881 GGAGAAATTT CTGTCCTCCA AACCACTGGC TGTCAGAACA AGAGGATATC AAAGCAGATA 
     2941 AAGTTACCCA GCTCTATGTA CCTGCATCTA GACATGTGTG GCGATTCCGG AGGATGGGCA 
     3001 GGATTGGCCC ACTGCAGTCC ACCCTGGAGG TGGGTCTGGA GTCCTTACCC CTGTCTGTGT 
     3061 CTGAGGAACG ACAGCTTGCC ATCCTGACAG AACTGCCTTT TGTGGTTCCA TTTGAGGAGC 
     3121 GAGTGAAGAT CTTTCAAAGG TTGATTTATG CTGATAAGCA AGAAGTTCAA GGCGATGGTC 
     3181 CATTTCTGGA TGGAATCAAT GTTACTATAA GGAGAAATTA TATTTATGAA GATGCTTATG 
     3241 ACAAACTTTC TCCAGAAAAT GAGCCTGATT TGAAAAAACG GATCCGGGTG CACTTGCTCA 
     3301 ATGCCCATGG CCTGGATGAA GCTGGCATCG ATGGTGGTGG TATTTTCAGA GAGTTTTTAA 
     3361 ATGAACTACT GAAATCTGGA TTTAACCCCA ACCAGGGCTT CTTTAAGACT ACTAATGAAG 
     3421 GGCTTCTATA CCCCAACCCA GCTGCTCAGA TGCTTGTGGG AGATTCTTTC GCAAGGCATT 
     3481 ACTACTTCCT AGGCAGAATG CTTGGAAAGG CTCTGTATGA AAACATGTTG GTGGAGCTAC 
     3541 CCTTTGCTGG CTTTTTCCTG TCCAAGCTAC TGGGAACCAG TGCAGATGTG GACATTCACC 
     3601 ATCTTGCCTC ATTGGACCCC GAAGTCTACA GGAACCTGCT TTTCCTCAAG AGCTATGAGG 
     3661 AGGATGTGGA GGAACTGGGA CTGAACTTTA CTGTGGTGAA CAATGACCTG GGAGAAGCCC 
     3721 AGGTGGTTGA ACTAAAATTT GGTGGAAAAG ACATCCCGGT AACTGGGGCC AACCGAATTG 
     3781 CCTACATCCA CCTGGTGGCT GACTACCGGC TGAACAAGCA GATCCGACCG CATTGCCTAG 
     3841 CTTTCCGCCA GGGCCTGGCC AATGTTGTGA GCCTAGAGTG GCTCCGAATG TTTGACCAAC 
     3901 AGGAAATTCA GGTATTAATT TCTGGTGCAC AAGTTCCTGT AAGTCTGGAG GACCTAAAAT 
     3961 CCTTTACAAA CTATTCAGGG GGCTATTCTG CCGATCATCC TGTCATTAAA ATCTTTTGGA 
     4021 GAGTTGTAGA AGGTTTCACT GACGAAGAAA AGCGCAAGTT GCTCAAGTTT GTAACAAGTT 
     4081 GCTCTCGCCC CCCTCTCCTG GGTTTCAAGG AGCTATACCC TGCGTTTTGC ATTCACAATG 
     4141 GAGGCTCTGA CCTTGAGCGG CTACCTACAG CCAGCACCTG CATGAACCTG CTGAAGCTCC 
     4201 CAGAGTTTTA TGATGAAGCC CTGCTGCGAA GCAAGCTGCT GTATGCCATT GAGTGTGCTG 
     4261 CTGGCTTTGA GCTGAGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4321 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4381 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4441 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4501 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4561 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4621 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4681 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4741 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4801 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4861 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4921 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4981 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5041 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5101 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5161 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5221 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5281 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5341 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5401 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5461 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5521 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5581 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5641 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5701 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5761 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5821 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5881 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5941 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6001 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6061 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6121 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6181 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6241 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6301 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6361 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6421 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6481 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6541 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6601 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6661 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6721 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6781 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6841 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6901 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6961 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7021 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7081 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7141 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7201 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7261 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7321 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7381 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7501 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7561 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7621 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7681 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7741 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7801 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7861 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7921 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7981 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8041 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8101 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//