LOCUS       MR223266                6783 bp    DNA     circular  SYN 
DEFINITION  Trim25 (Myc-DDK-tagged) - Mouse tripartite motif-containing 25 (Trim25)
ACCESSION   NM_009546
VERSION     NM_009546.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2940..3031 
                     /label="Myc-DDK"  
     misc_feature    3061..3646 
                     /label="PolyA signal"  
     misc_feature    3118..3137 
                     /label="Restriction Enzyme"  
     misc_feature    3922..4594 
                     /label="ColE1"  
     misc_feature    5024..5818 
                     /label="Kan/Neo"  
     misc_feature    5853..6155 
                     /label="SV40 ori"  
     misc_feature    6241..6718 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2922..2928 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2922 
                     /label="Trim25"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGCTG AATCCTCTGG CCGAGGAGCT GTCCTGCTCC GTCTGCCTGG 
     1081 AGCTCTTCAA GGAACCTGTC ACCACTCCGT GCGGCCACAA TTTCTGCATG TCATGCCTGG 
     1141 ATGAGACGTG GGTCGTCCAG GGCCCGCCGT ACCGATGCCC GCAATGTCGC AAAGTGTACC 
     1201 AGGTGCGCCC GCAGCTGCAA AAGAACACGG TAATGTGCGC GGTGGTGGAG CAGTTCCTGC 
     1261 AGGCCGAGCA GGCGCGGACC CCGGTGGACG ACTGGACGCC TCCTGCCCGC TTCTCCGCCT 
     1321 CCAGCGCAGC CACCCAGGTG GCCTGCGACC ACTGCCTGAC GGAGATCGCA GTGAAGACGT 
     1381 GCCTCGTGTG CATGGCCTCT TTCTGCCAGG AGCACTTGAG GCCTCACTTC GACAGTCCGG 
     1441 CCTTCCAGGA TCACCCCCTA CAGTCGCCCA TTCGTGACCT TCTGCGCCGC AAATGTACCC 
     1501 AGCACAACCG CCTTCGGGAA TTGTTTTGTC CCGAGCATGG CGAGTGCATC TGCCATATCT 
     1561 GCTTGGTAGA GCACAAGACC TGCTCCCCTA CGACCCTAAG TCAAGCCAGC GCCGACCTGG 
     1621 AGTACAAACT GAGGAATAAA CTCACTATCA TGCACAGCCA CATCAATGGG GCAACCAAAG 
     1681 CATTGGAGGA TGTGAGATCC AAGCAACAGT GTGTGCAGGA TTCTATGAAG AGGAAGATGG 
     1741 AACAGCTGAG ACAGGAGTAT ATGGAAATGA AGGCTGTCAT CGACGCAGCA GAGACCAGCT 
     1801 CCTTGCGCAA GTTAAAGGAG GAGGAGAAGA GAGTCTACGG AAAATTCGAC ACCATCTACC 
     1861 AGGTTCTCGT CAAGAAGAAG AGTGAGATGC AGAAACTGAA GGCAGAGGTT GAGCTCATTA 
     1921 TGGACAAAGG GGATGAGTTT GAGTTTCTGG AGAAAGCTGC AAAATTGCAA GGAGAGTCAA 
     1981 CAAAACCAGT CTACATCCCT AAGATAGATC TGGATCATGA TTTGATAATG GGAATCTACC 
     2041 AAGGGGCGGC GGACCTCAAG AGTGAACTGA AGCACTCCAT TAAGAAGCTA CAGAAGAAGT 
     2101 CTGAGGAGCA CAATGGCTCA GGTAACAAGG GAGACCAGAC ACAGTCCACT TTTAAACCCG 
     2161 TACAACCTTC GAAGAAGACC ATTCAAGAAA AGAAGACCAA GAAAACCCCT GTTGCTCCCG 
     2221 GCCCTCCCTC CCACTTCTCA CCTAACAAGT TGCCCACCTT TGGAGCTCCT GGCCAATCAC 
     2281 TGGATTCAAA AGCAACTTCC CCTGATGCCG CACCCAAAGC CAGTGCTGCA CAGCCAGACT 
     2341 CTGTAGGAGT CAAGGCTAAG GTGCTAGAAA ACTTCTTAAC CAAGTCCAGA ACGGAGCTTC 
     2401 TGGAGTATTT CGTGAAAGTC ATCTTCGACT ACAATACCGC CCACAACAAA GTGTCTCTGT 
     2461 CAAACAAGTA CACCACCGCC TCTGTGTCCG ATGGGCTCCA GCACTACCGG TCCCATCCCC 
     2521 AGAGGTTCAC CTACTGCTCT CAGGTCCTGG GGCTGCACTG CTACAAGAAC GGCATTCACT 
     2581 ACTGGGAGGT GGAACTACAG AAGAACAACT TCTGCGGCGT AGGCATCTGC TACGGCAGCA 
     2641 TGGAACGGCA GGGCCCCGAG AGCCGGCTGG GCCGCAACCC CAACTCATGG TGTGTGGAGT 
     2701 GGTTCAATAA CAAGATCTCT GCCTGGCACA ACAACGTGGA GAAGACTCTG CCCTCCACCA 
     2761 AGGCCACTCG AGTCGGCGTG CTGCTCAACT GTGACCACGG CTTCGTCATT TTCTTTGCGG 
     2821 TCACCGAAAA GGTGCACCTG ATGTACAAGT TCAAGGTGGA CTTCACCGAA GCTCTGTACC 
     2881 CAGCCTTCTG GGTGTTCTCG GCCGGTACCA CCCTCTCTAT CTGCTCCAAA ACGCGTACGC 
     2941 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3001 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3061 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3121 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3181 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3241 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3301 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     3361 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     3421 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     3481 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     3541 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     3601 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     3661 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     3721 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     3781 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     3841 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     3901 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     3961 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4021 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4081 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4141 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4201 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4261 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4321 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     4381 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     4441 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     4501 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     4561 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     4621 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     4681 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     4741 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     4801 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     4861 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     4921 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     4981 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5041 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5101 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5161 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5221 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5281 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     5341 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     5401 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     5461 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     5521 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     5581 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     5641 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     5701 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     5761 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     5821 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     5881 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     5941 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6001 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6061 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6121 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6181 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6241 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6301 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     6361 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     6421 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     6481 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     6541 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     6601 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     6661 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     6721 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     6781 TTT
//