LOCUS       MR223216                7956 bp    DNA     circular  SYN 
DEFINITION  Lnpep (Myc-DDK-tagged) - Mouse leucyl/cystinyl aminopeptidase (Lnpep)
ACCESSION   NM_172827
VERSION     NM_172827.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4113..4204 
                     /label="Myc-DDK"  
     misc_feature    4234..4819 
                     /label="PolyA signal"  
     misc_feature    4291..4310 
                     /label="Restriction Enzyme"  
     misc_feature    5095..5767 
                     /label="ColE1"  
     misc_feature    6197..6991 
                     /label="Kan/Neo"  
     misc_feature    7026..7328 
                     /label="SV40 ori"  
     misc_feature    7414..7891 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4095..4101 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4095 
                     /label="Lnpep"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTCCTTT ACCAATGATC GGCTTCAGCT TCCAAGGAAT ATGATCGAAA 
     1081 ACAGCATGTT TGAAGAAGAG CCAGATGTGG TAGATTTAGC CAAAGAACCT TGTTTACATC 
     1141 CTCTGGAACC CGATGAAGTG GAATATGAGC CCCGAGGTTC GAGGCTTCTG GTGCGAGGTC 
     1201 TTGGTGAGCA TGAGATGGAC GAGGATGAAG AGGATTATGA GTCCTCTGCG AAGCTGCTGG 
     1261 GCATGTCCTT CATGAACAGA AGCTCAGGCC TGCGGAACAG TGCAGCAGGC TACAGGCAGA 
     1321 GTCCAGATGG GACTTGTTCA TTACCCTCTG CCAGGACCTT AGTGATCTGT GTTTTTGTCA 
     1381 TTGTGGTTGC GGTCTCTGTA ATCATGGTGA TTTATCTACT GCCTAGATGT ACCTTTACCA 
     1441 AAGAAGGCTG CCACAAAACA AACCAGTCAG CAGAACTCAT CCAGCCAGTT GCTACAAACG 
     1501 GGAAAGTGTT CCCATGGGCT CAAATTAGGC TTCCCACTGC CATTATTCCT CTATGCTATG 
     1561 AACTTAGCCT ACATCCAAAC CTAACCTCAA TGACATTCAG GGGATCTGTG ACAATTTCAC 
     1621 TTCAGGCTCT TCAAGACACA CGGGATATCA TTCTCCATAG CACAGGACAT AATATTTCAA 
     1681 GAGTGACATT TATGTCAGCA GTTTCAAGTC AAGAAAAACA AGTTGAAATT CTGGAATATC 
     1741 CATATCATGA ACAAATCGCC GTTGTTGCCC CGGAACCTCT TCTAACAGGA CACAATTATA 
     1801 CCCTGAAGAT AGAGTATTCA GCAAATATAT CTAACTCTTA TTATGGGTTT TATGGCATCA 
     1861 CCTACACAGA TAAAAGTAAT GAGAAAAAGT ACTTTGCAGC AACTCAGTTT GAACCTCTGG 
     1921 CAGCAAGATC TGCTTTTCCT TGTTTTGATG AACCAGCATT TAAGGCCACA TTTATCATCA 
     1981 AGATCACAAG GAATGAGCAC CATACTGCGT TATCAAATAT GCCTAAGAAG TCATCGGTCC 
     2041 CTGCAGAAGA AGGACTTATT CAAGATGAGT TTTCTGAAAG TGTGAAAATG AGCACATACC 
     2101 TGGTTGCTTT CATTGTAGGG GAGATGAGGA ACCTGAGTCA GGATGTAAAT GGAACTCTGG 
     2161 TTTCTGTGTA TGCTGTACCA GAAAAAATTG GTCAAGTTCA CCATGCCTTG GACACAACTA 
     2221 TAAAGCTTCT TGAGTTTTAT CAAACTTACT TTGAAATTCA GTACCCACTT AAGAAATTGG 
     2281 ATTTGGTGGC CATTCCTGAC TTTGAAGCAG GAGCAATGGA AAACTGGGGC CTGCTTACAT 
     2341 TCCGAGAAGA GACTCTTCTG TATGACAATG CAACTTCTTC AGTAGCAGAC AGAAAACTGG 
     2401 TCACTAAAAT CATTGCTCAC GAACTGGCAC ATCAGTGGTT TGGAAATCTG GTTACAATGC 
     2461 AGTGGTGGAA TGACCTGTGG CTAAATGAAG GCTTTGCCAC TTTCATGGAG TATTTCTCTG 
     2521 TGGAAAAAAT ATTCAAAGAG CTCAACAGTT ATGAAGACTT CTTAGATGCT CGATTTAAAA 
     2581 CCATGAGGAA AGATTCCTTG AATTCGTCTC ATCCAATATC ATCATCTGTT CAGTCTTCGG 
     2641 AACAAATAGA AGAAATGTTT GATTCTCTCT CCTATTTTAA GGGAGCTTCT CTCTTGTTGA 
     2701 TGCTGAAAAG TTACCTTAGT GAAGATGTAT TTCGGCATGC TGTCATTCTT TACCTGCACA 
     2761 ATCACAGCTA TGCAGCTATC CAAAGTGATG ATCTCTGGGA CAGCTTCAAT GAGGTCACAG 
     2821 ACAAAACTCT AGATGTAAAG AAAATGATGA AAACCTGGAC CCTACAGAAA GGATTCCCGT 
     2881 TAGTGACAGT CCAGAGGAAG GGGACTGAGC TTCTTCTACA ACAGGAAAGA TTTTTTCTAC 
     2941 GCATGCAACC AGAAAGTCAG CCTTCAGATA CAAGCCACCT TTGGCATATT CCAATATCCT 
     3001 ATGTCACTGA TGGAAGAAAC TATTCAGAAT ATCGATCAGT TTCACTACTG GACAAGAAAT 
     3061 CAGATGTCAT CAATCTTACA GAACAAGTGC AGTGGGTCAA AGTCAATTCA AACATGACTG 
     3121 GCTATTACAT TGTTCACTAT GCTCATGATG ACTGGACAGC TCTAATCAAT CAGTTAAAAA 
     3181 GAAACCCTTA TGTTCTGAGT GACAAAGACC GAGCCAACCT GATCAATAAC ATCTTTGAAC 
     3241 TTGCAGGTCT TGGCAAAGTG CCTCTTCGGA TGGCATTTGA TTTGATTGAC TATCTTAAAA 
     3301 ATGAGACCCA CACTGCACCG ATCACTGAAG CCCTGTTCCA GACAAACCTT ATCTATAATC 
     3361 TCCTAGAAAA ACTGGGGCAC ATGGACCTGT CCTCAAGATT GGTGGCCAGA GTACATAAAT 
     3421 TGCTCCAGAA CCAAATCCAG CAGCAGACTT GGACAGATGA AGGCACACCA TCCATGCGAG 
     3481 AGCTTCGGTC AGCCTTGCTG GAATTTGCCT GTGCCCACAG CCTAGAGAAC TGTACCACTA 
     3541 TGGCCACAAA TCTGTTTGAC AGTTGGATGG CATCAAATGG AACTCAGAGC CTGCCTACTG 
     3601 ATGTCATGGT CACTGTGTTC AAGGTGGGAG CGAGAACCGA GAAAGGCTGG TTGTTCCTCT 
     3661 TTAGCATGTA TTCCTCCATG GGCTCTGAAG CAGAAAAGAA TAAAATTCTT GAAGCTCTTG 
     3721 CCAGCTCAGA GGATGTACAT AAACTTTACT GGTTAATGAA AAGTAGCCTT GATGGTGACA 
     3781 TCATCCGAAC ACAGAAGTTG TCACTTATCA TTAGAACAGT GGGCAGACAT TTTCCTGGAC 
     3841 ACTTGCTGGC TTGGGATTTT GTTAAAGAAA ACTGGAATAA GCTTGTACAT AAGTTCCATC 
     3901 TGGGCTCCTA TACCATACAA AGTATTGTTG CTGGATCTAC TCACTTATTT TCAACAAAGA 
     3961 CACATTTATC TGAGGTCCAG GCATTCTTTG AAAATCAGTC AGAGGCAACC TTGAAGCTTC 
     4021 GGTGTGTTCA GGAGGCTTTG GAAGTGATTC AGCTGAATAT CCAGTGGATG GTCAGGAATC 
     4081 TGAAAACGCT CTCCCAGTGG CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4141 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4201 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4261 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4321 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4381 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4441 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4501 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4561 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4621 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4681 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4741 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4801 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4861 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5761 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5821 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5881 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5941 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6001 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6061 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6121 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6181 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6241 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6301 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6361 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6421 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6481 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6541 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6601 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6661 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6721 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6781 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6841 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6901 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6961 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7021 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7081 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7141 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7201 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7381 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7501 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7561 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7621 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7681 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7741 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7801 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7861 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7921 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//