LOCUS       MR223174                8352 bp    DNA     circular  SYN 
DEFINITION  Nfasc (Myc-DDK-tagged) - Mouse neurofascin (Nfasc), transcript variant 4
ACCESSION   NM_001160318
VERSION     NM_001160318.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4509..4600 
                     /label="Myc-DDK"  
     misc_feature    4630..5215 
                     /label="PolyA signal"  
     misc_feature    4687..4706 
                     /label="Restriction Enzyme"  
     misc_feature    5491..6163 
                     /label="ColE1"  
     misc_feature    6593..7387 
                     /label="Kan/Neo"  
     misc_feature    7422..7724 
                     /label="SV40 ori"  
     misc_feature    7810..8287 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4491..4497 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4491 
                     /label="Nfasc"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAGGCAG CAGGCGCCAC CCTGGGTCCA CATAGCCCTC ATCCTCTTCC 
     1081 TCCTCAGCCT CGGAGGGGCC ATCGAGATTC CGATGGACCT GACCCAACCC CCAACTATCA 
     1141 CCAAGCAGTC CGTGAAGGAC CACATCGTGG ACCCTCGAGA TAACATCCTG ATTGAATGTG 
     1201 AAGCTAAAGG CAACCCCGCC CCCAGTTTTC ACTGGACTCG CAACAGCAGA TTCTTCAACA 
     1261 TTGCCAAGGA CCCACGGGTG TCCATGAGGA GGAGATCTGG GACCTTGGTG ATCGACTTCC 
     1321 GCAGTGGTGG GCGGCCTGAG GAATACGAAG GGGAGTACCA GTGCTTTGCC CGGAACAAAT 
     1381 TTGGCACTGC ACTTAGCAAC CGCATCCGCC TGCAGGTGTC CAAATCTCCC CTGTGGCCCA 
     1441 AGGAAAACCT AGACCCCGTC GTGGTTCAAG AGGGTGCCCC CTTGACACTA CAGTGCAACC 
     1501 CCCCACCCGG CCTCCCGTCC CCCGTCATCT TCTGGATGAG CAGCTCCATG GAGCCCATCA 
     1561 CCCAGGACAA GCGTGTCTCC CAGGGTCACA ACGGGGACCT GTACTTCTCC AACGTCATGC 
     1621 TGCAGGACAT GCAGACCGAC TACAGCTGCA ACGCGCGCTT TCACTTCACC CACACCATTC 
     1681 AGCAGAAGAA CCCCTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCA GAAAGAACGC 
     1741 CCAGCTTCAT GTATCCCCAG GGCACATCGA GCAGTCAGAT GGTTCTCCGT GGCATGGACC 
     1801 TGCTGCTGGA ATGCATTGCC TCTGGCGTCC CAACACCAGA CATTGCATGG TACAAGAAAG 
     1861 GTGGGGACCT CCCATCTAAC AAGGCCAAGT TCGAGAACTT TAATAAGGCT CTGCGCATCA 
     1921 CCAATGTCTC TGAAGAGGAC TCTGGGGAGT ATTTCTGCCT GGCCTCCAAC AAGATGGGCA 
     1981 GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC ATACTGGCTG GATGAGCCCA 
     2041 AGAACCTGAT CCTGGCTCCT GGGGAAGATG GGAGGCTGGT ATGCCGAGCC AATGGGAACC 
     2101 CGAAGCCGAC CGTGCAGTGG ATGGTGAATG GAGAGCCTTT ACAATCGGCA CCACCCAATC 
     2161 CCAACCGTGA GGTAGCTGGA GACACTATCA TCTTCCGGGA TACTCAGATC AGCAGCAGGG 
     2221 CAGTGTACCA ATGTAATACA TCCAATGAAC ATGGCTACCT GCTGGCCAAT GCCTTCGTCA 
     2281 GCGTGTTAGA TGTACCCCCT CGGATGCTGT CTGCCCGCAA CCAGCTCATC AGGGTGATCC 
     2341 TTTATAACCG GACACGGCTG GACTGTCCGT TCTTTGGGTC TCCCATCCCA ACACTCCGAT 
     2401 GGTTTAAGAA TGGGCAAGGA AGCAACCTGG ATGGCGGTAA CTACCACGTC TACGAAAACG 
     2461 GCAGTCTAGA AATCAAGATG ATTCGCAAAG AGGACCAAGG CATCTACACC TGTGTGGCCA 
     2521 CCAACATCCT GGGCAAAGCC GAAAATCAAG TCCGCCTGGA GGTCAAAGAC CCCACCAGGA 
     2581 TCTACAGGAT GCCCGAGGAC CAGGTGGCCA AGAGGGGCAC CACGGTGCAG CTGGAGTGCC 
     2641 GCGTGAAACA TGACCCCTCC TTGAAGCTCA CAGTCTCCTG GCTGAAGGAC GATGAGCCAC 
     2701 TCTACATTGG AAACAGGATG AAGAAGGAAG ATGACTCCCT GACGATCTTC GGAGTGGCAG 
     2761 AGCGGGACCA GGGCAGTTAC ACGTGTATGG CCAGCACCGA GCTGGACCAG GACCTGGCAA 
     2821 AGGCCTACCT CACTGTTCTA GGGCGACCAG ACCGACCCAG GGACCTGGAG CTCACTGACC 
     2881 TGGCTGAGAG GAGTGTGAGG CTGACCTGGA TCCCAGGGGA TGACAACAAC AGCCCTATCA 
     2941 CAGACTACGT CGTTCAGTTT GAAGAGGACC AGTTCCAACC AGGGGTGTGG CATGACCACT 
     3001 CCAGGTTCCC AGGCAGCGTC AACTCAGCCG TCCTCCATCT GTCCCCATAT GTCAACTACC 
     3061 AATTCAGAGT CATCGCTGTC AACGAGGTTG GGAGCAGCCA CCCCAGCCTT CCATCCGAGC 
     3121 GGTACCGAAC CAGTGGGGCA CCCCCTGAAT CTAATCCCAG TGATGTGAAG GGCGAAGGGA 
     3181 CAAGAAAGAA CAATATGGAG ATCACGTGGA CGCCTATGAA TGCTACCTCT GCCTTTGGCC 
     3241 CCAACCTACG CTACATTGTC AAGTGGCGAC GGAGAGAGAC CCGAGAGACT TGGAACAATG 
     3301 TCACAGTGTG GGGCTCTCGC TACGTGGTGG GGCAGACGCC TGTCTACGTT CCCTATGAGA 
     3361 TCCGAGTCCA GGCTGAAAAT GACTTTGGGA AAGGCCCCGA GCCTGACACC ATCATTGGGT 
     3421 ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACTGAAGT TAAAATCCGA GTCCTGAACA 
     3481 GCACAGCCAT CAGCCTTCAG TGGAACCGAG TCTACTCTGA CACGGTCCAG GGCCAGCTCA 
     3541 GAGAGTATCG AGCTTACTAC TGGAGGGAAA GCAGTTTGCT GAAGAACCTG TGGGTGTCTC 
     3601 AGAAGAGACA GCAGGCCAGC TTCCCTGGTG ACCGTCCCCG GGGCGTGGTG GCCCGCCTGT 
     3661 TCCCCTACAG TAACTACAAG CTGGAGATGG TGGTGGTCAA TGGGAGAGGT GACGGGCCTC 
     3721 GAAGTGAAAC CAAGGAATTC ACCACCCCAG AAGGAGTACC CAGTGCCCCC AGGCGGTTCA 
     3781 GAGTCCGACA GCCCAACCTG GAGACCATCA ACCTGGAGTG GGACCACCCA GAGCACCCCA 
     3841 ACGGAATCCT GATTGGATAC ATCCTCAGAT ACGTGCCCTT TAATGGAACC AAACTGGGAA 
     3901 AGCAGATGGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CTCTGTGCAG AGAGCAGACC 
     3961 CAGTGTCGCG TTACCGCTTC TCCCTCAGTG CCAGGACACA GGTGGGCTCT GGAGAAGCAG 
     4021 CCACAGAGGA GTCCCCAGCA CCTCCAAATG AAGCTACTCC AACTGCAGCT TACACCAATA 
     4081 ACCAGGCAGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGTGCC ATTGCCCTTC 
     4141 TGGTGCTGAT CCTTCTCATC GTCTGCTTCA TCAAGAGGAG TCGAGGTGGC AAGTACCCAG 
     4201 TGCGGGAAAA GAAGGATGTC CCCTTGGGTC CTGAAGACCC CAAAGAAGAA GATGGCTCAT 
     4261 TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCT CTGGATGGCA 
     4321 CCATCAAGCA GCAGGAGAGC GATGACAGCC TGGTGGACTA TGGCGAAGGC GGCGAGGGCC 
     4381 AGTTCAATGA AGATGGCTCC TTTATTGGCC AGTACACTGT CAAAAAGGAC AAGGAGGAAA 
     4441 CGGAGGGCAA TGAGAGCTCA GAGGCCACAT CACCAGTCAA TGCCATCTAT TCCCTTGCCA 
     4501 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4561 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4621 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4681 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4741 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4801 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4861 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4921 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4981 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5041 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5101 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5161 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5221 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5281 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5341 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5401 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5461 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5521 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5581 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5641 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5701 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5761 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5821 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5881 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5941 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6001 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6061 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6121 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6181 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6241 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6301 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6361 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6421 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6481 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6541 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6601 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6661 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6721 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6781 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6841 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6901 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6961 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7021 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7081 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7141 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7201 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7261 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7321 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7381 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7441 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7501 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7561 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7621 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7681 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7741 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7801 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7861 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7921 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7981 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8041 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8101 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8161 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8221 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8281 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8341 AACGCGAATT TT
//