LOCUS       MR223156                8973 bp    DNA     circular  SYN 
DEFINITION  Atad2 (Myc-DDK-tagged) - Mouse ATPase family, AAA domain containing 2 (Atad2)
ACCESSION   NM_027435
VERSION     NM_027435.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5130..5221 
                     /label="Myc-DDK"  
     misc_feature    5251..5836 
                     /label="PolyA signal"  
     misc_feature    5308..5327 
                     /label="Restriction Enzyme"  
     misc_feature    6112..6784 
                     /label="ColE1"  
     misc_feature    7214..8008 
                     /label="Kan/Neo"  
     misc_feature    8043..8345 
                     /label="SV40 ori"  
     misc_feature    8431..8908 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5112..5118 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5112 
                     /label="Atad2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGTCCTC CGCAGCAGCC TGGAGCTGCA CAGTCGCTCC ACCACCTCCT 
     1081 CGGCCACCGA CTCTCTGGAC CTGTCCAGTG AGTTCCTCAG CCTGCAGGAC AGCGGCGGGA 
     1141 GGCGGCCCAG ATTGCGCCGA GCCGGGAGGA AATCGACGGC GTCCAAGGCG GCGGGCGATG 
     1201 GATTCACAGT TAAGGGAGCT GAAACCTACC ACAACACGCG CTCTTTAAGG TCTCTGAGAA 
     1261 ACCAAGCACA AACTTCTTCA GAGTCTAGTT TTGACAAGAA CGTGGACAGA ACAGAGAAAT 
     1321 ACTCCAATGG CAGACATTTC ACAAGGCAAT TTGCTCGACA GCAGGCTGAT AAAAAAGAAG 
     1381 AGTGCAAAGA AGACAAAGTT ATTCCAGTTA TTCGCTCATT GAGGAATAGA AACATTGCTC 
     1441 CAACTGCAGA ACATTTGCAC GAACAAAGTG GAGATGTTGA AGTTCGAAGA AGTTGCAGGA 
     1501 TTAGAAGTCG TTACAGTACC ATGAACCAGT CTGTGCTATT TGACAAGCTT ATAACAAACA 
     1561 CTGCTGAAGC TGTACTTCAA AAGATGGATG ACATGAAAAA GATGCGCAGA CAGCGGATGA 
     1621 AGAAGCTTGA AGACTTGGGA GTATTCAATG AAACAGAAGA GAGTAATCTC ACTATGTACA 
     1681 CAAGAGGAAA ACTAAAGGCT ATCCAGCGAG CTGATGAAGA AACAACTGAT AACCAGGACG 
     1741 GCAGTGTGGA ATCATCTGAA GAGGGGGAAG AGCAAGAGGA TGATGATGGC GAAGATGAAG 
     1801 ATGATGAAGA TGAAGAAGAG GGAGAAGAAG ATAATCAAAA AAGATACTAT CTTAGGCAGA 
     1861 GAAAAACAAC GGTTTACTAC CAGTCTCCAC TGGAAAGTAA ACCTCGTCAC CAGAGAAAGC 
     1921 CCAACATGTT TTATAGTGGC CCAGCTTCTC CTGCAAGACC AAGATTCCGC TTGTCTTCTA 
     1981 CGGGACCAAG AAGTCCCTAT TGTAAACGAA TGAGCAGGCG AAGGCATGCC ATCCACAGCA 
     2041 GTGACTCTAC TTCCTCCTCC TCCTCTGAAG ACGACTGTTT TGAGAGGAGA ACAAAAAGGA 
     2101 ACCGAAATAG AGCTATTAAC AGGTGCCTCC CACTAAATTT TCGGAAAGAT GAAATAAGAG 
     2161 GGATTTATAA AGATCGAATG AAAATTGGAG CAAGCCTTGC TGATGTTGAC CCAATGCAAC 
     2221 TAGATACTTC AGTGCGCTTT GACAGTGTTG GCGGCCTGTC CAGTCACATT GCAGCTCTAA 
     2281 AAGAGATGGT GGTGTTTCCC TTACTTTATC CAGAAGTCTT CGAAAAGTTT AAAATTCAAC 
     2341 CCCCAAGAGG TTGTTTGTTT TATGGACCAC CTGGAACTGG AAAAACCCTG GTTGCTAGAG 
     2401 CACTTGCCAA TGAGTGCAGC CGAGGGGATA AGAGAGTGGC GTTCTTCATG AGGAAAGGTG 
     2461 CTGACTGCCT GAGTAAATGG GTAGGAGAAT CTGAGAGACA GCTTCGGTTG CTATTTGACC 
     2521 AGGCCTATCA GATGCGCCCA GCAATTATAT TCTTTGATGA AATTGATGGT TTGGCTCCAG 
     2581 TACGATCTAG CAGGCAAGAT CAGATTCACA GTTCTATTGT TTCAACCCTG TTAGCTCTTA 
     2641 TGGATGGTTT GGACAGCAGA GGAGAAATTG TGGTCATTGG AGCTACCAAC CGGCTAGATT 
     2701 CCATAGACCC TGCTTTACGG AGGCCCGGTC GGTTTGACAG AGAATTCCTT TTTAGTCTAC 
     2761 CTGATAAAAA TGCTCGAAAA GAGATTCTGA AGATTCATAC AAGAGATTGG AATCCAAAGC 
     2821 CAGTGGACAT GTTTCTAGAA GAACTAGCAG AACACTGTGT TGGCTACTGT GGTGCGGATA 
     2881 TTAAGTCAAT CTGTGCTGAA GCTGCTTTGT GCGCCCTGCG TCGACGGTAC CCGCAGATCT 
     2941 ACACCACCAG CGAGAAGCTT CAGCTGGATC TCTCTTCAAT TACCATCTCA GCTAAGGATT 
     3001 TTGAGGCAGC TCTGCAGAAA ATAAGACCAG CTTCCCAGAG AGCTGTGACA TCACCCGGAC 
     3061 AGGCACTGTC TGCCATTGTG AAACCACTTC TGCAAAATAC TGTTCATAGG ATCTTAGACG 
     3121 CCCTACAGAA AGTATTTCCA CATGTGGAAG TTGGAACAAA CAAATCTTTA AATTCAGATG 
     3181 TTTCTTGCCC TTTTCTAGAA AGTGATTTGG CATACAGTGA TGATGATACA CCATCTGTGT 
     3241 ATGAAAATGG ACTTTCTCAA AAAGAAAATT TAAATTTTCT TCATTTAAAT AGAAATGCCT 
     3301 GTTACCAGCC TATGTCTTTT CGACCAAGAC TATTGATAGT GGGAGAACCA GGATTTGGAC 
     3361 AGAGTTCTCA CTTGGCACCA GCTGTCATCC ATGCTTTGGA AAAATTTACT GTATATACAT 
     3421 TAGACATTCC TGTTCTTTTT GGCATCAGTA CAACATCTCC TGAAGAGGCA TGTTCCCAAA 
     3481 TGATTCGTGA AGCTAAGAGA ACAGCACCCA GCATAGTCTA TGTCCCACAT ATCCATTTGT 
     3541 GGTGGGAAAT AGTTGGACCG ACACTCAAAG CCACATTTAC AACATTATTA CAGACTATAC 
     3601 CTTCATTTGC TCCAGTTTTA TTACTTGCAA CTTCTGAGAA ACCATACTCA GCTTTGCCGG 
     3661 AAGAGGTACA AGAATTGTTT ACCCATGATT ATGGAGAAAT TTTTAATGTA CAGTTACCAG 
     3721 ATAAAGAAGA ACGAACCAAA TTTTTTGAAG ACTTAATTTT AAAACAAGCT TCAAAGCCTC 
     3781 CTGTGTCACA AAAGAAAGCA GTTCTGCAGG CCTTGGAGGT GCTCCCAGTC GCACCACCGC 
     3841 CTGAACCAAG ACCACTGACA GCAGAGGAAG TTAAACGGCT TGAAGAACAA GAAGAAGATA 
     3901 CATTCAGAGA ACTCAGGATT TTCTTAAGAA ATGTCACACA TAGACTTGCT ATTGATAAGC 
     3961 GATTCCGAGT CTTTACTAAG CCTGTTGACC CTGATGAGGT TCCTGATTAT GTCACTGTCA 
     4021 TAAAGCAACC AATGGACCTT TCATCTGTAA TCAGTAAAAT TGATTTACAT AAATATCTCA 
     4081 CTGTGAAAGA CTATTTGAAG GATATTGATC TAATCTGTAG TAATGCTTTA GAATACAATC 
     4141 CAGATAGAGA TCCTGGAGAT CGTCTTATTA GGCATAGAGC TTGTGCTTTG AGAGACACTG 
     4201 CTTATGCAAT AATTAAAGAG GAGCTTGATG AAGACTTTGA GCAGCTCTGT GAAGAAATTC 
     4261 AGGAATCTCG GAAGAAGAGA GGTTGTAGCT CCTCCAAATA CGCACCATCT TATTACCACG 
     4321 TCATGCCAAA GCAAAATTCT CCTCCTGTTG GTGATAAAAA GCCAGATCAA GAGCAGAATG 
     4381 AGAAGCTAAA GGTACCGTGC ACTCCTGTGG CGTGCAGCAC ACCTGCTCAG TTGAAAAGAA 
     4441 AATTCCATAA AAAGTCAAAG TGGCATGTAG GCACCAAAAT AAAGCGAAGG AAAATTTCAC 
     4501 AAGCTAAAGA CAACAGCTTG AATGCTATGA ACAGTTCAAG CAGGAGCGAC ACAGAAGACA 
     4561 GCCAGCACAC ACATGCAGAG CACACGGAGC CTGGAAACAC GGACGAGTCC TCGGTAGAAG 
     4621 AAAGTGACAA GCAAAATAGA CTTGAAAGCA ACATAGACTT GAAAAATAAC TCAAGTTCCT 
     4681 CTAATATTGA GAATGAACTT GAAGAGCCTA AGGAAACTAC GGAGGGCACA GAACTGAGAA 
     4741 AAGACAGGAT TGTTTGCAGG GGTGATGCCT CTGCCTCCCA GGTCACAGAC ATTCCCGAAG 
     4801 ACAGTGAATC AAAAGAAATG GATTTTCTGC GGATGACTCT AGCTAGAGGT TCCCAGGTAG 
     4861 AGCAGCAGGA GCTCATCAGT ATGGAGCAGG CTCTGGCCAT CCTCTCTCAG CCGACACCCT 
     4921 CACTTGTGCT GGACCACAAG CAGTTAACAA ATATTTTGAA GACGGTTGTT AAAAAAAGTC 
     4981 AGAAATATAA TATATTCCAA CTGGAGAATT TGTATGCAGT AATCAGCCAG TGTATTTATG 
     5041 AGCACCGCAG GGACTATGAC AAAACAGCGC TTGTTCAGAA AATGGAGCAA GCAGTAGAAA 
     5101 ACTTCAATTG TTCCAGATCA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5161 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5221 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5281 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5341 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5401 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5461 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5521 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5581 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5641 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5701 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5761 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5821 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5881 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5941 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6001 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6061 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6121 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6181 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6241 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6301 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6361 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6421 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6481 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6541 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6601 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6661 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6721 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6781 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6841 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6901 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6961 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7021 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7081 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7141 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7201 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7261 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7321 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7381 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7441 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7501 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7561 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7621 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7681 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7741 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7801 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7861 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7921 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7981 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8041 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8101 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8161 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8221 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8281 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8341 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8401 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8461 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8521 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8581 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8641 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8701 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8761 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8821 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8881 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8941 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//