LOCUS       MR223105                8937 bp    DNA     circular  SYN 
DEFINITION  Astn2 (Myc-DDK-tagged) - Mouse astrotactin 2 (Astn2), transcript variant 2
ACCESSION   NM_207109
VERSION     NM_207109.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5094..5185 
                     /label="Myc-DDK"  
     misc_feature    5215..5800 
                     /label="PolyA signal"  
     misc_feature    5272..5291 
                     /label="Restriction Enzyme"  
     misc_feature    6076..6748 
                     /label="ColE1"  
     misc_feature    7178..7972 
                     /label="Kan/Neo"  
     misc_feature    8007..8309 
                     /label="SV40 ori"  
     misc_feature    8395..8872 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5076..5082 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5076 
                     /label="Astn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCC GGCGCCCGGC GCAGCCCGGG CCGCGGCTTG GGGCTTCGGG 
     1081 GGCGGCCGAG GCTCGGCTTC CACCCGGGGC CGCCGCCGCC ACCGCCGCCG CCGCTGCTGT 
     1141 TGCTGTTTCT GCTCCTGCTG CCGCCGCCGC CGTTGCTGGC CGGCGCCACG GCCGCCGCCG 
     1201 CCTCGCGGGA GCCCGACAGC CCATGCCGGC TCAAGACCGT CACCGTATCC ACGCTGCCCG 
     1261 CTCTGCGGGA AAGCGACATC GGCTGGAGCG GTGCCCGCAC CGGGGCCGCG GCCGGGGCTG 
     1321 GGGCCGGGAC CGGAGCCGGG GCCGGAGCTG CTGCTGCTGC CGCCTCCGCC GCGTCCCCGG 
     1381 GCTCCGCGGG TTCAGCCGGC ACAGCCGCGG AGTCTCGTCT CCTTCTCTTT GTGCGTAATG 
     1441 AGCTCCCCGG GCGCATCGCG GTACAAGATG ACCTGGACAA CACTGAGCTG CCCTTCTTCA 
     1501 CCCTGGAGAT GTCTGGCACA GCAGCGGACA TTTCTTTGGT TCACTGGAGG CAGCAGTGGC 
     1561 TGGAGAATGG CACATTGTAC TTCCACGTTT CCATGAGCAG CTCTGGGCAA CTGGCTCAGG 
     1621 CCACTGCTCC CACACTCCAG GAGCCCTCGG AGATCGTTGA GGAACAGATG CATATCCTCC 
     1681 ACATTTCTGT GATGGGTGGA CTCATTGCAC TTCTCCTCCT GCTGTTGGTG TTCACAGTGG 
     1741 CACTGTATGC CCAGCGACGC TGGCAGAAGC GCCGGCGCAT CCCACAGAAG AGCGCAAGTA 
     1801 CAGAAGCTAC TCATGAGATT CACTACATCC CATCAGTGCT GCTGGGTCCT CAGGCCAGGG 
     1861 AAAGCTTCCG ATCCTCCAGA CTTCAGACAC ACAACTCAGT CATTGGTGTG CCTATCCGGG 
     1921 AAACCCCCAT CCTGGATGAC TATGACTATG AAGAGGAGGA GGAACCACCC AGGCGAGCCA 
     1981 ACCACGTCTC CCGTGAGGAT GAGTTTGGTA GCCAGATGAC CCATGCCCTG GACAGCTTGG 
     2041 GAAGGCCAGG AGAAGAGAAA GTGGAATTTG AGAAGAAAGC AGCAGCTGAG GCGACACAGG 
     2101 AAACAGTGGA GTCCCTGATG CAGAAGTTCA AGGAGAGTTT CCGTGCTAAC ACACCAGTGG 
     2161 AGATCGGTCA GTTGCAGCCA GCCTCGCGCA GCAGCACCTC TGCAGGGAAG AGGAAGCGGA 
     2221 GGAACAAATC TCGAGGGGGA ATCAGCTTTG GGAGAACCAA GGGGACATCA GGCTCAGAAG 
     2281 CAGATGACGA AACACAGCTG ACCTTCTACA CAGAGCAGTA CCGCAGTCGC CGCCGCAGCA 
     2341 AAGGTTTACT GAAAAGCCCT GTGAATAAGA CAGCCTTAAC ACTGATTGCT GTGAGCTCCT 
     2401 GCATCTTGGC CATGGTGTGT GGCAACCAGA TGTCCTGTCC ACTTACTGTG AAGGTGACTT 
     2461 TGCATGTGCC TGAACACTTC ATTGCAGATG GGAGCAGCTT TGTGGTGAGT GAAGGAAGCT 
     2521 ACCTGGATAT CTCCGATTGG CTAAACCCTG CCAAGCTGTC TCTGTATTAC CAGATCAATG 
     2581 CCACCTCGCC ATGGGTGAGA GACCTCTGTG GACAGAGGAC GACAGATGCC TGTGAACAGC 
     2641 TCTGTGACCC AGATACTGGG GAGTGCAGTT GTCATGAAGG TTACGCCCCT GACCCAGTCC 
     2701 ACAGACATCT ATGTGTTCGC AGTGACTGGG GTCAAAGTGA AGGACCTTGG CCTTACACAA 
     2761 CACTGGAGAG GGGCTATGAT CTGGTGACAG GAGAGCAAGC CCCTGAAAAG ATTCTCAGGT 
     2821 CTACTTTCAG CTTGGGCCAA GGCCTCTGGC TTCCGGTCAG CAAAAGCTTT GTGGTGCCAC 
     2881 CGGTGGAGCT GTCCATCAAC CCCCTGGCCA GCTGCAAGAC GGATGTGCTC GTCACAGAAG 
     2941 ACCCTGCGGA TGTCAGGGAA GAAGCAATGT TGTCCACGTA CTTTGAAACC ATCAACGACC 
     3001 TGCTGTCCTC CTTTGGTCCA GTCCGTGACT GCTCAAGGAA CAACGGGGGC TGTACCCGTA 
     3061 ACTTCAAGTG TGTGTCTGAC CGCCAGGTGG ACTCCTCGGG ATGTGTGTGT CCTGAGGAAC 
     3121 TGAAGCCCAT GAAGGATGGC TCTGGCTGCT ATGATCACTC CAAGGGCATC GACTGCTCTG 
     3181 ATGGCTTTAA TGGAGGGTGT GAGCAGCTTT GCCTACAACA AACACTGCCT TTGCCCTATG 
     3241 ATACCACCTC CAGCACCATC TTCATGTTCT GCGGCTGTGT GGAAGAGTAC AAGCTGGCTC 
     3301 CTGATGGGAA ATCCTGTCTA ATGCTCTCAG ATGTCTGTGA GGGCCCCAAG TGCCTCAAAC 
     3361 CTGACTCCAA ATTCAATGAC ACCCTCTTTG GAGAGATGCT ACATGGTTAC AACAACCGGA 
     3421 CACAACATGT AAACCAAGGC CAAGTCTTCC AAATGACATT CAGGGAGAAT AACTTCATCA 
     3481 AGGACTTTCC ACAGCTGGCA GATGGGCTGT TGGTGATCCC GCTGCCAGTG GAAGAGCAAT 
     3541 GCCGGGGGGT TCTCTCTGAG CCCCTTCCAG ACCTTCAGCT TCTCACAGGA GACATCAGGT 
     3601 ATGATGAGGC CATGGGGTAC CCCATGGTGC AACAGTGGCG TGTCCGGAGC AATCTCTATC 
     3661 GTGTGAAGCT GAGTACTATC ACCCTCTCAG CAGGCTTCAC CAATGTCCTA AAGATCCTGA 
     3721 CCAAAGAGAG CAGTCGGGAT GAGCTGCTGT CCTTCATCCA GCACTATGGC TCCCACTACA 
     3781 TTGCCGAGGC TTTATACGGT TCTGAGCTCA CCTGCATCAT CCATTTTCCC AGCAAGAAGG 
     3841 TCCAGCAGCA GCTATGGCTC CAATATCAGA AAGAGACCAC GGAGCTGGGC AGCAAGAAGG 
     3901 AGCTCAAGTC CATGCCCTTC ATCACCTACC TCTCCGGTCT CCTGACAGCC CAGATGCTAT 
     3961 CAGATGACCA GCTCATCTCA GGTGTGGAGA TTCGATGTGA GGAGAAGGGC CGCTGTCCAT 
     4021 CTACCTGTCA CCTTTGCCGC CGGCCAGGCA AAGAGCAGCT GAGCCCCACA CCAGTGCTGC 
     4081 TGGAGATAAA CCGTGTGGTT CCACTTTATA CCCTCATCCA AGACAATGGC ACAAAGGAGG 
     4141 CCTTCAAGAA TGCACTGATG AGTTCTTACT GGTGTTCTGG TAAAGGAGAT GTGATTGATG 
     4201 ACTGGTGCAG GTGTGACCTC AGTGCCTTTG ATGCCAGTGG GCTTCCAAAT TGTAGCCCCC 
     4261 TTCCACAGCC GGTGTTACGA CTTTCTCCAA CAGTGGAGCC TTCCAGCACT GTGGTTTCCC 
     4321 TGGAGTGGGT AGATGTTCAG CCAGCCATTG GAACCAAAGT CTCTGACTAT ATTCTGCAGC 
     4381 ACAAGAAAGT GGATGAATAC ACAGACACGG ACCTATACAC AGGAGAATTC CTGAGTTTTG 
     4441 CCGATGACTT ACTTTCTGGC CTGGGTACAT CTTGTGTAGC AGCTGGTCGA AGCCATGGAG 
     4501 AGGTTCCTGA AGTCAGTATC TACTCGGTGA TCTTCAAGTG CCTGGAGCCA GATGGTCTTT 
     4561 ACAAGTTCAC TCTGTATGCT GTGGATACTC GAGGGAGGCA CTCAGAGCTC AGCACAGTGA 
     4621 CTCTAAGGAC AGCTTGTCCA CTGGTAGATG ACAACAAAGC AGAAGAGATA GCGGACAAGA 
     4681 TTTACAATCT ATACAATGGT TACACAAGTG GCAAGGAGCA GCAGACTGCT TACAACACAC 
     4741 TGATGGAAGT CTCCGCCTCC ATGCTCTTCC GTGTCCAGCA CCACTACAAC TCTCACTATG 
     4801 AAAAGTTTGG TGACTTCGTC TGGAGGAGTG AGGATGAGCT AGGACCAAGG AAGGCTCATC 
     4861 TGATTCTGCG GCGGCTGGAG CGGGTCAGCA GCCACTGTTC TAGTCTCTTG AGAAGCGCCT 
     4921 ACATCCAGAG TCGTGTGGAC ACCATACCCT ATCTCTTCTG CCGCAGTGAG GAGGTCCGGC 
     4981 CTGCAGGAAT GGTGTGGTAC AGCATCCTCA AAGACACCAA AATCACATGT GAGGAGAAGA 
     5041 TGGTGTCTAT GGCCCGAAAC ACGTACGGGG AAACCAAAGG CCGGACGCGT ACGCGGCCGC 
     5101 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5161 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5221 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5281 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5341 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5401 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5461 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5521 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5581 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5641 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5701 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5761 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5821 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5881 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5941 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6001 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6061 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6121 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6181 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6241 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6301 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6361 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6421 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6481 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6541 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6601 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6661 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6721 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6781 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6841 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6901 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6961 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7021 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7081 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7141 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7201 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7261 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7321 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7381 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7441 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7501 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7561 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7621 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7681 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7741 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7801 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7861 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7921 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7981 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8041 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8101 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8161 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8221 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8281 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8341 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8401 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8461 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8521 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8581 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8641 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8701 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8761 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8821 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8881 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//