LOCUS       MR223104                8781 bp    DNA     circular  SYN 
DEFINITION  Astn2 (Myc-DDK-tagged) - Mouse astrotactin 2 (Astn2), transcript variant 1
ACCESSION   NM_019514
VERSION     NM_019514.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4938..5029 
                     /label="Myc-DDK"  
     misc_feature    5059..5644 
                     /label="PolyA signal"  
     misc_feature    5116..5135 
                     /label="Restriction Enzyme"  
     misc_feature    5920..6592 
                     /label="ColE1"  
     misc_feature    7022..7816 
                     /label="Kan/Neo"  
     misc_feature    7851..8153 
                     /label="SV40 ori"  
     misc_feature    8239..8716 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4920..4926 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4920 
                     /label="Astn2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGCCGCC GGCGCCCGGC GCAGCCCGGG CCGCGGCTTG GGGCTTCGGG 
     1081 GGCGGCCGAG GCTCGGCTTC CACCCGGGGC CGCCGCCGCC ACCGCCGCCG CCGCTGCTGT 
     1141 TGCTGTTTCT GCTCCTGCTG CCGCCGCCGC CGTTGCTGGC CGGCGCCACG GCCGCCGCCG 
     1201 CCTCGCGGGA GCCCGACAGC CCATGCCGGC TCAAGACCGT CACCGTATCC ACGCTGCCCG 
     1261 CTCTGCGGGA AAGCGACATC GGCTGGAGCG GTGCCCGCAC CGGGGCCGCG GCCGGGGCTG 
     1321 GGGCCGGGAC CGGAGCCGGG GCCGGAGCTG CTGCTGCTGC CGCCTCCGCC GCGTCCCCGG 
     1381 GCTCCGCGGG TTCAGCCGGC ACAGCCGCGG AGTCTCGTCT CCTTCTCTTT GTGCGTAATG 
     1441 AGCTCCCCGG GCGCATCGCG GTACAAGATG ACCTGGACAA CACTGAGCTG CCCTTCTTCA 
     1501 CCCTGGAGAT GTCTGGCACA GCAGCGGACA TTTCTTTGGT TCACTGGAGG CAGCAGTGGC 
     1561 TGGAGAATGG CACATTGTAC TTCCACGTTT CCATGAGCAG CTCTGGGCAA CTGGCTCAGG 
     1621 CCACTGCTCC CACACTCCAG GAGCCCTCGG AGATCGTTGA GGAACAGATG CATATCCTCC 
     1681 ACATTTCTGT GATGGGTGGA CTCATTGCAC TTCTCCTCCT GCTGTTGGTG TTCACAGTGG 
     1741 CACTGTATGC CCAGCGACGC TGGCAGAAGC GCCGGCGCAT CCCACAGAAG AGCGCAAGTA 
     1801 CAGAAGCTAC TCATGAGATT CACTACATCC CATCAGTGCT GCTGGGTCCT CAGGCCAGGG 
     1861 AAAGCTTCCG ATCCTCCAGA CTTCAGACAC ACAACTCAGT CATTGGTGTG CCTATCCGGG 
     1921 AAACCCCCAT CCTGGATGAC TATGACTATG AAGAGGAGGA GGAACCACCC AGGCGAGCCA 
     1981 ACCACGTCTC CCGTGAGGAT GAGTTTGGTA GCCAGATGAC CCATGCCCTG GACAGCTTGG 
     2041 GAAGGCCAGG AGAAGAGAAA GTGGAATTTG AGAAGAAAGG GGGAATCAGC TTTGGGAGAA 
     2101 CCAAGGGGAC ATCAGGCTCA GAAGCAGATG ACGAAACACA GCTGACCTTC TACACAGAGC 
     2161 AGTACCGCAG TCGCCGCCGC AGCAAAGGTT TACTGAAAAG CCCTGTGAAT AAGACAGCCT 
     2221 TAACACTGAT TGCTGTGAGC TCCTGCATCT TGGCCATGGT GTGTGGCAAC CAGATGTCCT 
     2281 GTCCACTTAC TGTGAAGGTG ACTTTGCATG TGCCTGAACA CTTCATTGCA GATGGGAGCA 
     2341 GCTTTGTGGT GAGTGAAGGA AGCTACCTGG ATATCTCCGA TTGGCTAAAC CCTGCCAAGC 
     2401 TGTCTCTGTA TTACCAGATC AATGCCACCT CGCCATGGGT GAGAGACCTC TGTGGACAGA 
     2461 GGACGACAGA TGCCTGTGAA CAGCTCTGTG ACCCAGATAC TGGGGAGTGC AGTTGTCATG 
     2521 AAGGTTACGC CCCTGACCCA GTCCACAGAC ATCTATGTGT TCGCAGTGAC TGGGGTCAAA 
     2581 GTGAAGGACC TTGGCCTTAC ACAACACTGG AGAGGGGCTA TGATCTGGTG ACAGGAGAGC 
     2641 AAGCCCCTGA AAAGATTCTC AGGTCTACTT TCAGCTTGGG CCAAGGCCTC TGGCTTCCGG 
     2701 TCAGCAAAAG CTTTGTGGTG CCACCGGTGG AGCTGTCCAT CAACCCCCTG GCCAGCTGCA 
     2761 AGACGGATGT GCTCGTCACA GAAGACCCTG CGGATGTCAG GGAAGAAGCA ATGTTGTCCA 
     2821 CGTACTTTGA AACCATCAAC GACCTGCTGT CCTCCTTTGG TCCAGTCCGT GACTGCTCAA 
     2881 GGAACAACGG GGGCTGTACC CGTAACTTCA AGTGTGTGTC TGACCGCCAG GTGGACTCCT 
     2941 CGGGATGTGT GTGTCCTGAG GAACTGAAGC CCATGAAGGA TGGCTCTGGC TGCTATGATC 
     3001 ACTCCAAGGG CATCGACTGC TCTGATGGCT TTAATGGAGG GTGTGAGCAG CTTTGCCTAC 
     3061 AACAAACACT GCCTTTGCCC TATGATACCA CCTCCAGCAC CATCTTCATG TTCTGCGGCT 
     3121 GTGTGGAAGA GTACAAGCTG GCTCCTGATG GGAAATCCTG TCTAATGCTC TCAGATGTCT 
     3181 GTGAGGGCCC CAAGTGCCTC AAACCTGACT CCAAATTCAA TGACACCCTC TTTGGAGAGA 
     3241 TGCTACATGG TTACAACAAC CGGACACAAC ATGTAAACCA AGGCCAAGTC TTCCAAATGA 
     3301 CATTCAGGGA GAATAACTTC ATCAAGGACT TTCCACAGCT GGCAGATGGG CTGTTGGTGA 
     3361 TCCCGCTGCC AGTGGAAGAG CAATGCCGGG GGGTTCTCTC TGAGCCCCTT CCAGACCTTC 
     3421 AGCTTCTCAC AGGAGACATC AGGTATGATG AGGCCATGGG GTACCCCATG GTGCAACAGT 
     3481 GGCGTGTCCG GAGCAATCTC TATCGTGTGA AGCTGAGTAC TATCACCCTC TCAGCAGGCT 
     3541 TCACCAATGT CCTAAAGATC CTGACCAAAG AGAGCAGTCG GGATGAGCTG CTGTCCTTCA 
     3601 TCCAGCACTA TGGCTCCCAC TACATTGCCG AGGCTTTATA CGGTTCTGAG CTCACCTGCA 
     3661 TCATCCATTT TCCCAGCAAG AAGGTCCAGC AGCAGCTATG GCTCCAATAT CAGAAAGAGA 
     3721 CCACGGAGCT GGGCAGCAAG AAGGAGCTCA AGTCCATGCC CTTCATCACC TACCTCTCCG 
     3781 GTCTCCTGAC AGCCCAGATG CTATCAGATG ACCAGCTCAT CTCAGGTGTG GAGATTCGAT 
     3841 GTGAGGAGAA GGGCCGCTGT CCATCTACCT GTCACCTTTG CCGCCGGCCA GGCAAAGAGC 
     3901 AGCTGAGCCC CACACCAGTG CTGCTGGAGA TAAACCGTGT GGTTCCACTT TATACCCTCA 
     3961 TCCAAGACAA TGGCACAAAG GAGGCCTTCA AGAATGCACT GATGAGTTCT TACTGGTGTT 
     4021 CTGGTAAAGG AGATGTGATT GATGACTGGT GCAGGTGTGA CCTCAGTGCC TTTGATGCCA 
     4081 GTGGGCTTCC AAATTGTAGC CCCCTTCCAC AGCCGGTGTT ACGACTTTCT CCAACAGTGG 
     4141 AGCCTTCCAG CACTGTGGTT TCCCTGGAGT GGGTAGATGT TCAGCCAGCC ATTGGAACCA 
     4201 AAGTCTCTGA CTATATTCTG CAGCACAAGA AAGTGGATGA ATACACAGAC ACGGACCTAT 
     4261 ACACAGGAGA ATTCCTGAGT TTTGCCGATG ACTTACTTTC TGGCCTGGGT ACATCTTGTG 
     4321 TAGCAGCTGG TCGAAGCCAT GGAGAGGTTC CTGAAGTCAG TATCTACTCG GTGATCTTCA 
     4381 AGTGCCTGGA GCCAGATGGT CTTTACAAGT TCACTCTGTA TGCTGTGGAT ACTCGAGGGA 
     4441 GGCACTCAGA GCTCAGCACA GTGACTCTAA GGACAGCTTG TCCACTGGTA GATGACAACA 
     4501 AAGCAGAAGA GATAGCGGAC AAGATTTACA ATCTATACAA TGGTTACACA AGTGGCAAGG 
     4561 AGCAGCAGAC TGCTTACAAC ACACTGATGG AAGTCTCCGC CTCCATGCTC TTCCGTGTCC 
     4621 AGCACCACTA CAACTCTCAC TATGAAAAGT TTGGTGACTT CGTCTGGAGG AGTGAGGATG 
     4681 AGCTAGGACC AAGGAAGGCT CATCTGATTC TGCGGCGGCT GGAGCGGGTC AGCAGCCACT 
     4741 GTTCTAGTCT CTTGAGAAGC GCCTACATCC AGAGTCGTGT GGACACCATA CCCTATCTCT 
     4801 TCTGCCGCAG TGAGGAGGTC CGGCCTGCAG GAATGGTGTG GTACAGCATC CTCAAAGACA 
     4861 CCAAAATCAC ATGTGAGGAG AAGATGGTGT CTATGGCCCG AAACACGTAC GGGGAAACCA 
     4921 AAGGCCGGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4981 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5041 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5101 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5161 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5221 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5281 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5341 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5401 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5461 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5521 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5581 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5641 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5761 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5821 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5881 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5941 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6061 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6121 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6181 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6241 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6361 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6421 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6481 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6541 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6601 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6661 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6721 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6781 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6841 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6901 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6961 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7021 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7081 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7141 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7201 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7261 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7321 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7381 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7441 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7501 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7561 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7621 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7681 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7741 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7801 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7861 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7921 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7981 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8221 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8281 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8341 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8401 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8461 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8521 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8581 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8641 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8701 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8761 CAAAAATTTA ACGCGAATTT T
//