LOCUS       MR223090                8781 bp    DNA     circular  SYN 
DEFINITION  Mapk8ip3 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3), transcript variant 7
ACCESSION   NM_001163453
VERSION     NM_001163453.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4938..5029 
                     /label="Myc-DDK"  
     misc_feature    5059..5644 
                     /label="PolyA signal"  
     misc_feature    5116..5135 
                     /label="Restriction Enzyme"  
     misc_feature    5920..6592 
                     /label="ColE1"  
     misc_feature    7022..7816 
                     /label="Kan/Neo"  
     misc_feature    7851..8153 
                     /label="SV40 ori"  
     misc_feature    8239..8716 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4920..4926 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4920 
                     /label="Mapk8ip3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGAGATC CAGATGGACG AGGGAGGAGG AGTGGTGGTG TACCAAGACG 
     1081 ACTACTGCTC GGGCTCGGTC ATGTCGGAGC GTGTGTCGGG CCTGGCGGGC TCCATCTACC 
     1141 GCGAGTTCGA GCGCCTCATT CACTGCTATG ACGAGGAGGT GGTCAAGGAG CTCATGCCGC 
     1201 TGGTGGTGAA CGTGCTGGAG AACCTTGACT CGGTGCTGAG CGAGAACCAG GAGCACGAGG 
     1261 TGGAGCTGGA GCTCCTACGC GAGGACAACG AGCAGCTGCT CACGCAATAC GAGCGCGAGA 
     1321 AGGCGCTGCG CAAACAGGCC GAGGAGAAAT TCATCGAATT TGAAGATGCC TTGGAACAAG 
     1381 AGAAGAAAGA ACTCCAGATC CAGGTAGAAC ATTATGAGTT TCAGACACGC CAGCTGGAGC 
     1441 TAAAGGCCAA AAACTATGCA GATCAGATTT CCCGACTGGA GGAACGAGAA TCGGAGATGA 
     1501 AGAAGGAATA CAATGCCCTG CACCAGCGGC ACACAGAGAT GATCCAGACC TATGTGGAAC 
     1561 ACATTGAAAG ATCCAAGATG CAGCAAGTTG GGGGTAGCGG CCAAACAGAA AGCAGCCTGC 
     1621 CCGGGCGGAG CAGGAAGGAG CGTCCCACCT CTCTGAATGT CTTCCCCCTG GCTGATGGCA 
     1681 TGTGTCCAAA TGATGAGATG TCTGAGTCAG GCCAGTCCTC AGCAGCTGCA ACACCCAGTA 
     1741 CCGCAGGTAC CAAGTCCAAC ACACCCACGT CCTCCGTGCC CTCAGCAGCA GTCACGCCAC 
     1801 TCAACGAGAG CCTACAGCCC CTGGGGGACT ATGTCAGTGT CACAAAGAAC AACAAGCAGG 
     1861 CCCGAGAGAA GCGCAATAGC CGTAACATGG AGGTCCAGGT CACCCAAGAG ATGCGGAACG 
     1921 TCAGTATCGG CATGGGCAGC AGTGACGAGT GGTCCGATGT TCAGGACATT ATCGACTCCA 
     1981 CCCCAGAGCT GGATGTGTGT CCTGAAACCC GTCTGGAGCG CACAGGAAGC AGCCCAACCC 
     2041 AGGGAATTGT AAACAAAGCT TTTGGAATCA ACACTGACTC CTTGTATCAC GAACTCTCCA 
     2101 CGGCGGGATC TGAGGTCATC GGGGATGTGG ACGAGGGAGC TGATCTCCTA GGGGAGTTTT 
     2161 CAGGAATGGG CAAAGAAGTG GGGAACCTGC TGCTGGAGAA CTCACAGCTT CTAGAGACAA 
     2221 AAAATGCTTT AAATGTAGTG AAGAATGACC TCATTGCTAA GGTTGACCAA CTGTCAGGAG 
     2281 AACAGGAGGT CCTGAAGGGT GAGCTGGAAG CAGCCAAGCA AGCCAAAGTC AAGCTGGAGA 
     2341 ACCGAATCAA AGAGCTTGAA GAAGAACTGA AGAGAGTCAA GTCAGAGGCA GTAACTGCCC 
     2401 GCCGTGAGCC CAGAGAAGAG GTGGAGGATG TAAGCAGCTA TCTCTGTACA GAATTGGACA 
     2461 AAATCCCCAT GGCCCAGCGC CGACGCTTCA CACGGGTGGA GATGGCCCGA GTGCTCATGG 
     2521 AACGCAACCA GTACAAGGAA CGCCTCATGG AGCTGCAGGA GGCTGTGAGG TGGACTGAAA 
     2581 TGATCAGAGC ATCAAGGGAA CACCCATCTG TCCAGGAGAA GAAGAAGTCC ACCATCTGGC 
     2641 AGTTCTTTAG TCGCCTCTTC AGCTCCTCAT CTAGCCCCCC TCCGGCCAAA CGATCCTACC 
     2701 CATCTGTGAA CATTCACTAC AAGTCACCCA CTGCAGCTGG CTTTAGCCAG CGTCGCAGCC 
     2761 ATGCTTTGTG CCAGATCTCA GCCGGCAGCA GGCCCCTGGA GTTCTTCCCT GATGATGACT 
     2821 GCACCTCTTC TGCCCGGCGG GAGCAGAAGC GGGAGCAGTA CCGCCAGGTT CGTGAACACG 
     2881 TGCGCAATGA TGACGGGAGG CTGCAGGCCT GTGGGTGGAG CCTGCCTGCC AAGTACAAGC 
     2941 AGCTGAGCCC CAATGGAGGC CAGGAAGACA CCCGGATGAA AAATGTGCCT GTCCCTGTGT 
     3001 ACTGCCGCCC TCTGGTGGAG AAGGACCCTT CGACAAAGCT GTGGTGTGCT GCTGGTGTCA 
     3061 ACCTGAGTGG GTGGAAGCCA CATGAAGAGG ACTCTAGCAA TGGACCCAAG CCTGTACCAG 
     3121 GTCGAGACCC TCTGACCTGT GACCGGGAAG GAGAAGGCGA ACCCAAGAGC ACACACCCAT 
     3181 CACCTGAGAA GAAGAAGGCA AAGGAAACCC CTGAGGCAGA TGCTACCTCC AGTCGGGTAT 
     3241 GGATCCTCAC CAGCACCCTG ACAACCAGCA AGGTGGTGAT CATTGATGCC AACCAGCCAG 
     3301 GCACAATTGT GGATCAGTTC ACAGTCTGCA ATGCCCACGT CCTGTGTATC TCCAGCATTC 
     3361 CTGCGGCCAG TGACAGTGAC TATCCCCCTG GGGAGATGTT CCTAGACAGT GATGTGAACC 
     3421 CTGAAGATTC AGGTGCTGAT GGTGTGCTGG CTGGCATCAC CCTGGTGGGG TGTGCTACCC 
     3481 GCTGCAATGT TCCACGTAGC AACTGTTCCT CACGAGGAGA CACCCCAGTA CTGGACAAGG 
     3541 GGCAGGGGGA TGTGGCGACC ACTGCCAATG GGAAGGTCAA CCCGTCCCAA TCCACAGAAG 
     3601 AAGCCACAGA AGCCACGGAG GTGCCAGACC CTGGTCCCAG CGAGTCAGAA GCAACGACAG 
     3661 TCCGGCCCGG GCCTCTCACA GAGCATGTCT TTACTGACCC AGCACCCACC CCATCCTCCA 
     3721 GCACCCAGCC TGCCAGTGAG AATGGGTCAG AGTCCAATGG CACCATTGTA CAGCCTCAGG 
     3781 TGGAGCCCAG TGGGGAACTC TCAACAACAA CCAGTAGCGC TGCACCCACT ATGTGGCTAG 
     3841 GAGCCCAGAA TGGCTGGCTC TATGTGCATT CAGCGGTAGC CAACTGGAAG AAGTGTCTGC 
     3901 ACTCCATCAA GCTAAAAGAC TCTGTGCTGA GCCTGGTGCA TGTCAAAGGC CGAGTGCTGG 
     3961 TAGCTCTTGC AGATGGGACC CTGGCTATCT TCCATCGTGG AGAGGATGGC CAGTGGGACC 
     4021 TGAGCAACTA CCACCTAATG GACCTGGGCC ACCCACACCA CTCCATCCGC TGCATGGCTG 
     4081 TTGTGAATGA CCGAGTTTGG TGTGGCTACA AGAACAAGGT GCATGTTATC CAGCCCAAGA 
     4141 CAATGCAGAT TGAGAAATCA TTTGATGCCC ACCCAAGGCG GGAAAGCCAG GTACGTCAGC 
     4201 TGGCCTGGAT CGGTGATGGA GTGTGGGTCT CTATTCGCTT GGATTCTACC CTTCGGCTCT 
     4261 ACCATGCTCA CACCCACCAG CACCTGCAGG ATGTGGACAT TGAGCCCTAT GTTAGCAAGA 
     4321 TGCTAGGAAC CGGCAAGCTG GGCTTCTCCT TCGTGCGCAT CACAGCCTTA CTCATTGCAG 
     4381 GCAACCGTCT GTGGGTGGGC ACTGGCAATG GGGTTGTCAT CTCCATCCCC TTGACTGAGA 
     4441 CTGTGGTCCT GCATCGAGGC CAGCTCCTAG GGCTCCGAGC CAACAAGACA TCCCCAACAT 
     4501 CTGGGGAGGG GACCCGCCCA GGGGGCATCA TCCATGTGTA TGGGGACGAC AGCAGTGACA 
     4561 AGGCCGCCAG TAGTTTCATC CCCTACTGCT CCATGGCACA GGCTCAGCTT TGCTTCCATG 
     4621 GGCACCGTGA TGCTGTCAAA TTCTTTGTCT CTGTGCCAGG AAATGTGCTG GCCACTCTCA 
     4681 ATGGCAGTGT GCTAGACAGC CCATCAGAGG GCCCTGGGCC TGCTGCACCC GCTGCAGATG 
     4741 CTGAGGGCCA GAAGTTGAAG AATGCACTGG TGCTGAGTGG TGGTGAAGGT TACATTGACT 
     4801 TCCGTATCGG AGACGGAGAG GATGATGAAA CTGAGGAATG TGCCGGGGAC GTGAACCAGA 
     4861 CAAAGCCCTC GTTGTCCAAG GCTGAGCGCA GCCACATCAT CGTGTGGCAG GTGTCCTACA 
     4921 CCCCTGAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4981 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5041 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5101 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5161 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5221 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5281 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5341 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5401 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5461 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5521 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5581 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5641 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5701 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5761 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5821 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5881 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5941 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6001 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6061 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6121 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6181 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6241 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6301 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6361 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6421 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6481 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6541 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6601 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6661 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6721 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6781 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6841 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6901 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6961 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7021 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7081 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7141 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7201 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7261 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7321 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7381 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7441 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7501 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7561 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7621 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7681 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7741 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7801 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7861 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7921 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7981 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8041 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8101 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8161 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8221 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8281 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8341 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8401 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8461 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8521 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8581 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8641 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8701 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8761 CAAAAATTTA ACGCGAATTT T
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