LOCUS       MR223086                8796 bp    DNA     circular  SYN 
DEFINITION  Mapk8ip3 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3), transcript variant 6
ACCESSION   NM_001163451
VERSION     NM_001163451.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4953..5044 
                     /label="Myc-DDK"  
     misc_feature    5074..5659 
                     /label="PolyA signal"  
     misc_feature    5131..5150 
                     /label="Restriction Enzyme"  
     misc_feature    5935..6607 
                     /label="ColE1"  
     misc_feature    7037..7831 
                     /label="Kan/Neo"  
     misc_feature    7866..8168 
                     /label="SV40 ori"  
     misc_feature    8254..8731 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4935..4941 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4935 
                     /label="Mapk8ip3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGAGATC CAGATGGACG AGGGAGGAGG AGTGGTGGTG TACCAAGACG 
     1081 ACTACTGCTC GGGCTCGGTC ATGTCGGAGC GTGTGTCGGG CCTGGCGGGC TCCATCTACC 
     1141 GCGAGTTCGA GCGCCTCATT CACTGCTATG ACGAGGAGGT GGTCAAGGAG CTCATGCCGC 
     1201 TGGTGGTGAA CGTGCTGGAG AACCTTGACT CGGTGCTGAG CGAGAACCAG GAGCACGAGG 
     1261 TGGAGCTGGA GCTCCTACGC GAGGACAACG AGCAGCTGCT CACGCAATAC GAGCGCGAGA 
     1321 AGGCGCTGCG CAAACAGGCC GAGGAGAAAT TCATCGAATT TGAAGATGCC TTGGAACAAG 
     1381 AGAAGAAAGA ACTCCAGATC CAGGTAGAAC ATTATGAGTT TCAGACACGC CAGCTGGAGC 
     1441 TAAAGGCCAA AAACTATGCA GATCAGATTT CCCGACTGGA GGAACGAGAA TCGGAGATGA 
     1501 AGAAGGAATA CAATGCCCTG CACCAGCGGC ACACAGAGAT GATCCAGACC TATGTGGAAC 
     1561 ACATTGAAAG ATCCAAGATG CAGCAAGTTG GGGGTAGCGG CCAAACAGAA AGCAGCCTGC 
     1621 CCGGGCGGAG GAAGGAGCGT CCCACCTCTC TGAATGTCTT CCCCCTGGCT GATGGCATGT 
     1681 GTCCAAATGA TGAGATGTCT GAGTCAGGCC AGTCCTCAGC AGCTGCAACA CCCAGTACCA 
     1741 CAGGTACCAA GTCCAACACA CCCACGTCCT CCGTGCCCTC AGCAGCAGTC ACGCCACTCA 
     1801 ACGAGAGCCT ACAGCCCCTG GGGGACTATG TCAGTGTCAC AAAGAACAAC AAGCAGGCCC 
     1861 GAGAGAAGCG CAATAGCCGT AACATGGAGG TCCAGGTCAC CCAAGAGATG CGGAACGTCA 
     1921 GTATCGGCAT GGGCAGCAGT GACGAGTGGT CCGATGTTCA GGACATTATC GACTCCACCC 
     1981 CAGAGCTGGA TGTGTGTCCT GAAACCCGTC TGGAGCGCAC AGGAAGCAGC CCAACCCAGG 
     2041 GAATTGTAAA CAAAGCTTTT GGAATCAACA CTGACTCCTT GTATCACGAA CTCTCCACGG 
     2101 CGGGATCTGA GGTCATCGGG GATGTGGACG AGGGAGCTGA TCTCCTAGGG GAGTTTTCAG 
     2161 TGCGCGATGA TTTTTTTGGA ATGGGCAAAG AAGTGGGGAA CCTGCTGCTG GAGAACTCAC 
     2221 AGCTTCTAGA GACAAAAAAT GCTTTAAATG TAGTGAAGAA TGACCTCATT GCTAAGGTTG 
     2281 ACCAACTGTC AGGAGAACAG GAGGTCCTGA AGGGTGAGCT GGAAGCAGCC AAGCAAGCCA 
     2341 AAGTCAAGCT GGAGAACCGA ATCAAAGAGC TTGAAGAAGA ACTGAAGAGA GTCAAGTCAG 
     2401 AGGCAGTAAC TGCCCGCCGT GAGCCCAGAG AAGAGGTGGA GGATGTAAGC AGCTATCTCT 
     2461 GTACAGAATT GGACAAAATC CCCATGGCCC AGCGCCGACG CTTCACACGG GTGGAGATGG 
     2521 CCCGAGTGCT CATGGAACGC AACCAGTACA AGGAACGCCT CATGGAGCTG CAGGAGGCTG 
     2581 TGAGGTGGAC TGAAATGATC AGAGCATCAA GGGAACACCC ATCTGTCCAG GAGAAGAAGA 
     2641 AGTCCACCAT CTGGCAGTTC TTTAGTCGCC TCTTCAGCTC CTCATCTAGC CCCCCTCCGG 
     2701 CCAAACGATC CTACCCATCT GTGAACATTC ACTACAAGTC ACCCACTGCA GCTGGCTTTA 
     2761 GCCAGCGTCG CAGCCATGCT TTGTGCCAGA TCTCAGCCGG CAGCAGGCCC CTGGAGTTCT 
     2821 TCCCTGATGA TGACTGCACC TCTTCTGCCC GGCGGGAGCA GAAGCGGGAG CAGTACCGCC 
     2881 AGGTTCGTGA ACACGTGCGC AATGATGACG GGAGGCTGCA GGCCTGTGGG TGGAGCCTGC 
     2941 CTGCCAAGTA CAAGCAGCTG AGCCCCAATG GAGGCCAGGA AGACACCCGG ATGAAAAATG 
     3001 TGCCTGTCCC TGTGTACTGC CGCCCTCTGG TGGAGAAGGA CCCTTCGACA AAGCTGTGGT 
     3061 GTGCTGCTGG TGTCAACCTG AGTGGGTGGA AGCCACATGA AGAGGACTCT AGCAATGGAC 
     3121 CCAAGCCTGT ACCAGGTCGA GACCCTCTGA CCTGTGACCG GGAAGGAGAA GGCGAACCCA 
     3181 AGAGCACACA CCCATCACCT GAGAAGAAGA AGGCAAAGGA AACCCCTGAG GCAGATGCTA 
     3241 CCTCCAGTCG GGTATGGATC CTCACCAGCA CCCTGACAAC CAGCAAGGTG GTGATCATTG 
     3301 ATGCCAACCA GCCAGGCACA ATTGTGGATC AGTTCACAGT CTGCAATGCC CACGTCCTGT 
     3361 GTATCTCCAG CATTCCTGCG GCCAGTGACA GTGACTATCC CCCTGGGGAG ATGTTCCTAG 
     3421 ACAGTGATGT GAACCCTGAA GATTCAGGTG CTGATGGTGT GCTGGCTGGC ATCACCCTGG 
     3481 TGGGGTGTGC TACCCGCTGC AATGTTCCAC GTAGCAACTG TTCCTCACGA GGAGACACCC 
     3541 CAGTACTGGA CAAGGGGCAG GGGGATGTGG CGACCACTGC CAATGGGAAG GTCAACCCGT 
     3601 CCCAATCCAC AGAAGAAGCC ACAGAAGCCA CGGAGGTGCC AGACCCTGGT CCCAGCGAGT 
     3661 CAGAAGCAAC GACAGTCCGG CCCGGGCCTC TCACAGAGCA TGTCTTTACT GACCCAGCAC 
     3721 CCACCCCATC CTCCAGCACC CAGCCTGCCA GTGAGAATGG GTCAGAGTCC AATGGCACCA 
     3781 TTGTACAGCC TCAGGTGGAG CCCAGTGGGG AACTCTCAAC AACAACCAGT AGCGCTGCAC 
     3841 CCACTATGTG GCTAGGAGCC CAGAATGGCT GGCTCTATGT GCATTCAGCG GTAGCCAACT 
     3901 GGAAGAAGTG TCTGCACTCC ATCAAGCTAA AAGACTCTGT GCTGAGCCTG GTGCATGTCA 
     3961 AAGGCCGAGT GCTGGTAGCT CTTGCAGATG GGACCCTGGC TATCTTCCAT CGTGGAGAGG 
     4021 ATGGCCAGTG GGACCTGAGC AACTACCACC TAATGGACCT GGGCCACCCA CACCACTCCA 
     4081 TCCGCTGCAT GGCTGTTGTG AATGACCGAG TTTGGTGTGG CTACAAGAAC AAGGTGCATG 
     4141 TTATCCAGCC CAAGACAATG CAGATTGAGA AATCATTTGA TGCCCACCCA AGGCGGGAAA 
     4201 GCCAGGTACG TCAGCTGGCC TGGATCGGTG ATGGAGTGTG GGTCTCTATT CGCTTGGATT 
     4261 CTACCCTTCG GCTCTACCAT GCTCACACCC ACCAGCACCT GCAGGATGTG GACATTGAGC 
     4321 CCTATGTTAG CAAGATGCTA GGAACCGGCA AGCTGGGCTT CTCCTTCGTG CGCATCACAG 
     4381 CCTTACTCAT TGCAGGCAAC CGTCTGTGGG TGGGCACTGG CAATGGGGTT GTCATCTCCA 
     4441 TCCCCTTGAC TGAGACTGTG GTCCTGCATC GAGGCCAGCT CCTAGGGCTC CGAGCCAACA 
     4501 AGACATCCCC AACATCTGGG GAGGGGACCC GCCCAGGGGG CATCATCCAT GTGTATGGGG 
     4561 ACGACAGCAG TGACAAGGCC GCCAGTAGTT TCATCCCCTA CTGCTCCATG GCACAGGCTC 
     4621 AGCTTTGCTT CCATGGGCAC CGTGATGCTG TCAAATTCTT TGTCTCTGTG CCAGGAAATG 
     4681 TGCTGGCCAC TCTCAATGGC AGTGTGCTAG ACAGCCCATC AGAGGGCCCT GGGCCTGCTG 
     4741 CACCCGCTGC AGATGCTGAG GGCCAGAAGT TGAAGAATGC ACTGGTGCTG AGTGGTGGTG 
     4801 AAGGTTACAT TGACTTCCGT ATCGGAGACG GAGAGGATGA TGAAACTGAG GAATGTGCCG 
     4861 GGGACGTGAA CCAGACAAAG CCCTCGTTGT CCAAGGCTGA GCGCAGCCAC ATCATCGTGT 
     4921 GGCAGGTGTC CTACACCCCT GAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4981 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5041 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5101 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5161 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5221 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5281 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5341 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5401 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5461 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5521 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5581 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5641 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5701 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5761 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5821 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5881 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5941 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6001 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6061 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6121 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6181 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6241 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6301 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6361 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6421 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6481 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6541 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6601 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6661 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6721 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6781 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6841 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6901 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6961 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7021 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7081 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7141 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7201 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7261 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7321 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7381 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7441 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7501 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7561 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7621 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7681 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7741 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7801 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7861 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7921 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7981 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8041 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8101 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8161 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8221 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8281 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8341 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8401 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8461 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8521 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8581 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8641 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8701 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8761 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//