LOCUS       MR223075                8847 bp    DNA     circular  SYN 
DEFINITION  Mapk8ip3 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3), transcript variant 3
ACCESSION   NM_001163448
VERSION     NM_001163448.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5004..5095 
                     /label="Myc-DDK"  
     misc_feature    5125..5710 
                     /label="PolyA signal"  
     misc_feature    5182..5201 
                     /label="Restriction Enzyme"  
     misc_feature    5986..6658 
                     /label="ColE1"  
     misc_feature    7088..7882 
                     /label="Kan/Neo"  
     misc_feature    7917..8219 
                     /label="SV40 ori"  
     misc_feature    8305..8782 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4986..4992 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4986 
                     /label="Mapk8ip3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGAGATC CAGATGGACG AGGGAGGAGG AGTGGTGGTG TACCAAGACG 
     1081 ACTACTGCTC GGGCTCGGTC ATGTCGGAGC GTGTGTCGGG CCTGGCGGGC TCCATCTACC 
     1141 GCGAGTTCGA GCGCCTCATT CACTGCTATG ACGAGGAGGT GGTCAAGGAG CTCATGCCGC 
     1201 TGGTGGTGAA CGTGCTGGAG AACCTTGACT CGGTGCTGAG CGAGAACCAG GAGCACGAGG 
     1261 TGGAGCTGGA GCTCCTACGC GAGGACAACG AGCAGCTGCT CACGCAATAC GAGCGCGAGA 
     1321 AGGCGCTGCG CAAACAGGCC GAGGAGAAAT TCATCGAATT TGAAGATGCC TTGGAACAAG 
     1381 AGAAGAAAGA ACTCCAGATC CAGGTAGAAC ATTATGAGTT TCAGACACGC CAGCTGGAGC 
     1441 TAAAGGCCAA AAACTATGCA GATCAGATTT CCCGACTGGA GGAACGAGAA TCGGAGATGA 
     1501 AGAAGGAATA CAATGCCCTG CACCAGCGGC ACACAGAGAT GATCCAGACC TATGTGGAAC 
     1561 ACATTGAAAG ATCCAAGATG CAGCAAGTTG GGGGTAGCGG CCAAACAGAA AGCAGCCTGC 
     1621 CCGGGCGGAG CAGGAAGGAG CGTCCCACCT CTCTGAATGT CTTCCCCCTG GCTGATGGCA 
     1681 TGGTACGTGC ACAGATGGGG GGCAAGCTCG TGCCTGCGGG GGACCACTGG CACCTGAGTG 
     1741 ACCTCGGCCA GCTACAGTCC AGCTCCAGCT ACCAGTGTCC AAATGATGAG ATGTCTGAGT 
     1801 CAGGCCAGTC CTCAGCAGCT GCAACACCCA GTACCACAGG TACCAAGTCC AACACACCCA 
     1861 CGTCCTCCGT GCCCTCAGCA GCAGTCACGC CACTCAACGA GAGCCTACAG CCCCTGGGGG 
     1921 ACTATGTCAG TGTCACAAAG AACAACAAGC AGGCCCGAGA GAAGCGCAAT AGCCGTAACA 
     1981 TGGAGGTCCA GGTCACCCAA GAGATGCGGA ACGTCAGTAT CGGCATGGGC AGCAGTGACG 
     2041 AGTGGTCCGA TGTTCAGGAC ATTATCGACT CCACCCCAGA GCTGGATGTG TGTCCTGAAA 
     2101 CCCGTCTGGA GCGCACAGGA AGCAGCCCAA CCCAGGGAAT TGTAAACAAA GCTTTTGGAA 
     2161 TCAACACTGA CTCCTTGTAT CACGAACTCT CCACGGCGGG ATCTGAGGTC ATCGGGGATG 
     2221 TGGACGAGGG AGCTGATCTC CTAGGGGAGT TTTCAGGAAT GGGCAAAGAA GTGGGGAACC 
     2281 TGCTGCTGGA GAACTCACAG CTTCTAGAGA CAAAAAATGC TTTAAATGTA GTGAAGAATG 
     2341 ACCTCATTGC TAAGGTTGAC CAACTGTCAG GAGAACAGGA GGTCCTGAAG GGTGAGCTGG 
     2401 AAGCAGCCAA GCAAGCCAAA GTCAAGCTGG AGAACCGAAT CAAAGAGCTT GAAGAAGAAC 
     2461 TGAAGAGAGT CAAGTCAGAG GCAGTAACTG CCCGCCGTGA GCCCAGAGAA GAGGTGGAGG 
     2521 ATGACAAAAT CCCCATGGCC CAGCGCCGAC GCTTCACACG GGTGGAGATG GCCCGAGTGC 
     2581 TCATGGAACG CAACCAGTAC AAGGAACGCC TCATGGAGCT GCAGGAGGCT GTGAGGTGGA 
     2641 CTGAAATGAT CAGAGCATCA AGGGAACACC CATCTGTCCA GGAGAAGAAG AAGTCCACCA 
     2701 TCTGGCAGTT CTTTAGTCGC CTCTTCAGCT CCTCATCTAG CCCCCCTCCG GCCAAACGAT 
     2761 CCTACCCATC TGTGAACATT CACTACAAGT CACCCACTGC AGCTGGCTTT AGCCAGCGTC 
     2821 GCAGCCATGC TTTGTGCCAG ATCTCAGCCG GCAGCAGGCC CCTGGAGTTC TTCCCTGATG 
     2881 ATGACTGCAC CTCTTCTGCC CGGCGGGAGC AGAAGCGGGA GCAGTACCGC CAGGTTCGTG 
     2941 AACACGTGCG CAATGATGAC GGGAGGCTGC AGGCCTGTGG GTGGAGCCTG CCTGCCAAGT 
     3001 ACAAGCAGCT GAGCCCCAAT GGAGGCCAGG AAGACACCCG GATGAAAAAT GTGCCTGTCC 
     3061 CTGTGTACTG CCGCCCTCTG GTGGAGAAGG ACCCTTCGAC AAAGCTGTGG TGTGCTGCTG 
     3121 GTGTCAACCT GAGTGGGTGG AAGCCACATG AAGAGGACTC TAGCAATGGA CCCAAGCCTG 
     3181 TACCAGGTCG AGACCCTCTG ACCTGTGACC GGGAAGGAGA AGGCGAACCC AAGAGCACAC 
     3241 ACCCATCACC TGAGAAGAAG AAGGCAAAGG AAACCCCTGA GGCAGATGCT ACCTCCAGTC 
     3301 GGGTATGGAT CCTCACCAGC ACCCTGACAA CCAGCAAGGT GGTGATCATT GATGCCAACC 
     3361 AGCCAGGCAC AATTGTGGAT CAGTTCACAG TCTGCAATGC CCACGTCCTG TGTATCTCCA 
     3421 GCATTCCTGC GGCCAGTGAC AGTGACTATC CCCCTGGGGA GATGTTCCTA GACAGTGATG 
     3481 TGAACCCTGA AGATTCAGGT GCTGATGGTG TGCTGGCTGG CATCACCCTG GTGGGGTGTG 
     3541 CTACCCGCTG CAATGTTCCA CGTAGCAACT GTTCCTCACG AGGAGACACC CCAGTACTGG 
     3601 ACAAGGGGCA GGGGGATGTG GCGACCACTG CCAATGGGAA GGTCAACCCG TCCCAATCCA 
     3661 CAGAAGAAGC CACAGAAGCC ACGGAGGTGC CAGACCCTGG TCCCAGCGAG TCAGAAGCAA 
     3721 CGACAGTCCG GCCCGGGCCT CTCACAGAGC ATGTCTTTAC TGACCCAGCA CCCACCCCAT 
     3781 CCTCCAGCAC CCAGCCTGCC AGTGAGAATG GGTCAGAGTC CAATGGCACC ATTGTACAGC 
     3841 CTCAGGTGGA GCCCAGTGGG GAACTCTCAA CAACAACCAG TAGCGCTGCA CCCACTATGT 
     3901 GGCTAGGAGC CCAGAATGGC TGGCTCTATG TGCATTCAGC GGTAGCCAAC TGGAAGAAGT 
     3961 GTCTGCACTC CATCAAGCTA AAAGACTCTG TGCTGAGCCT GGTGCATGTC AAAGGCCGAG 
     4021 TGCTGGTAGC TCTTGCAGAT GGGACCCTGG CTATCTTCCA TCGTGGAGAG GATGGCCAGT 
     4081 GGGACCTGAG CAACTACCAC CTAATGGACC TGGGCCACCC ACACCACTCC ATCCGCTGCA 
     4141 TGGCTGTTGT GAATGACCGA GTTTGGTGTG GCTACAAGAA CAAGGTGCAT GTTATCCAGC 
     4201 CCAAGACAAT GCAGATTGAG AAATCATTTG ATGCCCACCC AAGGCGGGAA AGCCAGGTAC 
     4261 GTCAGCTGGC CTGGATCGGT GATGGAGTGT GGGTCTCTAT TCGCTTGGAT TCTACCCTTC 
     4321 GGCTCTACCA TGCTCACACC CACCAGCACC TGCAGGATGT GGACATTGAG CCCTATGTTA 
     4381 GCAAGATGCT AGGAACCGGC AAGCTGGGCT TCTCCTTCGT GCGCATCACA GCCTTACTCA 
     4441 TTGCAGGCAA CCGTCTGTGG GTGGGCACTG GCAATGGGGT TGTCATCTCC ATCCCCTTGA 
     4501 CTGAGACTGT GGTCCTGCAT CGAGGCCAGC TCCTAGGGCT CCGAGCCAAC AAGACATCCC 
     4561 CAACATCTGG GGAGGGGACC CGCCCAGGGG GCATCATCCA TGTGTATGGG GACGACAGCA 
     4621 GTGACAAGGC CGCCAGTAGT TTCATCCCCT ACTGCTCCAT GGCACAGGCT CAGCTTTGCT 
     4681 TCCATGGGCA CCGTGATGCT GTCAAATTCT TTGTCTCTGT GCCAGGAAAT GTGCTGGCCA 
     4741 CTCTCAATGG CAGTGTGCTA GACAGCCCAT CAGAGGGCCC TGGGCCTGCT GCACCCGCTG 
     4801 CAGATGCTGA GGGCCAGAAG TTGAAGAATG CACTGGTGCT GAGTGGTGGT GAAGGTTACA 
     4861 TTGACTTCCG TATCGGAGAC GGAGAGGATG ATGAAACTGA GGAATGTGCC GGGGACGTGA 
     4921 ACCAGACAAA GCCCTCGTTG TCCAAGGCTG AGCGCAGCCA CATCATCGTG TGGCAGGTGT 
     4981 CCTACACCCC TGAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5041 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5101 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5161 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5221 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5281 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5341 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5401 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5461 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5521 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5581 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5641 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5701 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5761 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5821 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5881 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5941 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6001 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6061 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6121 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6181 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6241 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6301 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6361 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6421 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6481 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6541 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6601 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6661 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6721 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6781 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6841 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6901 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6961 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7021 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7081 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7141 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7201 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7261 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7321 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7381 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7441 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7501 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7561 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7621 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7681 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7741 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7801 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7861 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7921 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7981 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8041 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8101 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8161 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8221 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8281 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8341 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8401 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8461 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8521 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8581 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8641 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8701 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8761 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8821 ATTTAACAAA AATTTAACGC GAATTTT
//