LOCUS       MR223032                8421 bp    DNA     circular  SYN 
DEFINITION  Pcdh12 (Myc-DDK-tagged) - Mouse protocadherin 12 (Pcdh12)
ACCESSION   NM_017378
VERSION     NM_017378.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4578..4669 
                     /label="Myc-DDK"  
     misc_feature    4699..5284 
                     /label="PolyA signal"  
     misc_feature    4756..4775 
                     /label="Restriction Enzyme"  
     misc_feature    5560..6232 
                     /label="ColE1"  
     misc_feature    6662..7456 
                     /label="Kan/Neo"  
     misc_feature    7491..7793 
                     /label="SV40 ori"  
     misc_feature    7879..8356 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4560..4566 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4560 
                     /label="Pcdh12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCTACTT CTGCCATTCC TGCTAGGGCT CTTAGGGCCA GGAAGCTACT 
     1081 TGTTCATTTC AGGGGATTGT CAGGAGGTGG CCACTGTCAT GGTGAAATTC CAAGTGACAG 
     1141 AGGAAGTGCC GTCTGGCACG GTGATAGGGA AACTGTCCCA AGAACTAAGA GTGGAGGAGA 
     1201 GGCGTGGGAA GGCAGGAGAT GCCTTCCAGA TTCTGCAGCT GCCTCAGGCA CTGCCGGTTC 
     1261 AGATGAACTC TGAGGACGGC CTGCTCAGCA CTTCCAGCCG GCTGGATCGG GAGAAGCTAT 
     1321 GTCGGCAGGA AGATCCCTGT CTGGTGTCAT TTGACGTGCT TGCCACAGGG GCGTCTGCTC 
     1381 TAATTCATGT GGAGATTCAG GTGCTAGACA TCAATGACCA CCAGCCACAG TTTCCCAAAG 
     1441 ACGAGCAGGA ACTGGAAATC TCAGAGAGTG CCTCTCTGCA CACACGAATC CCCTTGGACA 
     1501 GAGCTCTTGA CCAAGACACG GGTCCTAACA GCTTATATTC CTACTCCCTG TCTCCCAGTG 
     1561 AACACTTTGC CCTGGATGTT ATTGTGGGCC CTGATGAGAC CAAACATGCA GAGCTTGTGG 
     1621 TGGTGAAGGA GTTGGACAGG GAACTCCACT CATATTTTGA TCTGGTGCTG ACCGCCTATG 
     1681 ACAATGGGAA TCCCCCTAAG TCAGGAATCA GCGTGGTCAA GGTCAATGTC CTGGACTCCA 
     1741 ATGACAATAG TCCAGTGTTT GCTGAGAGTT CACTAGCACT AGAAATCCCA GAAGACACTG 
     1801 TTCCTGGTAC TCTTCTCATA AACCTGACTG CTACAGATCC CGACCAAGGA CCCAATGGGG 
     1861 AGGTAGAGTT CTTCTTTGGC AAGCATGTGT CCCCAGAGGT GATGAACACC TTTGGCATAG 
     1921 ATGCCAAGAC AGGCCAGATC ATTCTGCGCC AAGCCCTAGA TTACGAGAAG AACCCTGCCT 
     1981 ATGAGGTGGA TGTCCAGGCA AGGGATTTGG GTCCCAATTC CATCCCAGGC CATTGCAAAG 
     2041 TTCTTATCAA AGTTCTGGAT GTCAATGACA ATGCCCCAAG CATCCTCATC ACGTGGGCCT 
     2101 CCCAGACGTC GCTGGTGTCA GAAGATCTTC CCAGGGATAG CTTCATTGCC CTTGTCAGTG 
     2161 CGAATGACTT GGACTCAGGA AACAACGGTC TCGTCCACTG TTGGCTGAAT CAAGAGCTGG 
     2221 GCCACTTCAG ACTGAAAAGG ACTAACGGCA ACACGTACAT GCTGCTCACC AATGCCACAC 
     2281 TGGACAGAGA GCAGTGGCCC ATATATACTC TCACTGTGTT TGCCCAAGAC CAAGGACCCC 
     2341 AGCCCTTATC AGCTGAGAAG GAGCTCCAAA TTCAGGTTAG TGATGTCAAT GACAATGCCC 
     2401 CTGTGTTTGA GAAGAGCCGG TACGAGGTCT CCACTTGGGA AAATAACCCA CCCTCTCTTC 
     2461 ACCTCATCAC GCTCAAAGCG CATGATGCTG ACTTGGGCAG TAATGGAAAA GTGTCATACC 
     2521 GTATCAAGGA CTCCCCCGTT TCTCACTTAG TCATTATTGA CTTTGAAACA GGAGAAGTCA 
     2581 CTGCTCAGAG GTCACTGGAC TATGAACAGA TGGCAGGCTT TGAGTTCCAG GTGATAGCAG 
     2641 AGGACAGAGG GCAACCCCAG CTCGCATCCA GCATCTCGGT GTGGGTTAGC CTCTTGGATG 
     2701 CCAATGATAA TGCCCCAGAA GTGATTCAGC CTGTGCTCAG TGAAGGCAAA GCCACCCTTT 
     2761 CGGTGCTTGT AAATGCCTCC ACGGGCCACC TTCTGTTGCC CATTGAGAAT CCCAGTGGCA 
     2821 TGGATCCAGC AGGTACTGGT ATACCACCAA AGGCTACCCA CAGCCCCTGG TCTTTCCTTT 
     2881 TGTTAACAAT CGTGGCTAGG GATGCAGACT CGGGGGCCAA TGGGGAACTC TTCTACAGCA 
     2941 TTCAAAGTGG GAATGATGCT CATCTCTTTT TCCTCAGCCC TTCCTTGGGG CAGCTATTCA 
     3001 TTAATGTCAC CAATGCCAGC AGCCTCATCG GGAGTCAGTG GGACCTGGGG ATAGTGGTAG 
     3061 AGGACCAGGG CAGCCCCTCC TTGCAGACCC AAGTTTCATT GAAGGTCGTG TTTGTCACCA 
     3121 GTGTGGACCA CCTAAGGGAT TCTGCTCATG AGCCCGGAGT TCTGAGCACA CCAGCACTGG 
     3181 CTTTGATCTG CCTGGCTGTA CTGCTGGCCA TCTTTGGATT GCTCTTAGCC CTGTTCGTGT 
     3241 CCATCTGCAG GACAGAGAGA AAGGATAATA GGGCCTACAA CTGTCGAGAA GCTGAGTCGT 
     3301 CATACCGCCA CCAGCCCAAG AGGCCCCAGA AACACATTCA GAAGGCAGAT ATCCACCTGG 
     3361 TGCCTGTGCT TAGGGCCCAC GAGAATGAGA CTGATGAAGT CAGGCCATCT CACAAGGATA 
     3421 CCAGCAAGGA GACACTGATG GAGGCAGGCT GGGACTCTTG CCTGCAGGCC CCCTTCCACC 
     3481 TCACACCAAC CCTATACAGG ACCCTGCGTA ACCAAGGCAA CCAGGGAGAA CTGGCAGAGA 
     3541 GCCAGGAGGT ACTGCAGGAC ACCTTCAACT TTCTCTTTAA CCATCCCAGG CAGAGGAATG 
     3601 CCTCCCGGGA GAACCTAAAC CTTCCTGAGT CCCCACCTGC TGTACGCCAA CCACTCTTAA 
     3661 GGCCTCTGAA GGTGCCTGGT AGCCCCATAG CGAGGGCGAC TGGAGACCAA GACAAGGAGG 
     3721 AGGCCCCACA GAGCCCACCA GCGTCCTCTG CAACCCTAAG ACGACAGCGG AATTTCAATG 
     3781 GCAAAGTGTC TCCTAGAGGA GAGTCCGGTC CTCATCAGAT TCTGAGGAGC CTGGTTAGGC 
     3841 TCTCTGTGGC TGCTTTTGCG GAACGGAACC CGGTGGAGGA GCCTGCTGGG GACTCTCCTC 
     3901 CTGTCCAGCA AATCTCCCAG CTGCTGTCCT TGCTGCACCA GGGCCAATTC CAGCCCAAAC 
     3961 CAAACCACCG AGGAAATAAA TACTTGGCCA AGCCCGGCGG CAGCAGCAGG GGTACCATCC 
     4021 CAGACACAGA GGGCCTTGTA GGCCTCAAGC CTAGTGGTCA AGCAGAACCT GACCTGGAAG 
     4081 AAGGGCCCCC GAGCCCGGAG GAGGACCTTT CTGTAAAGCG ACTTCTAGAA GAAGAGCTGT 
     4141 CGAGCCTGTT GGACCCTAAT ACAGGTCTAG CCCTGGACAA GCTGAGTCCG CCTGACCCAG 
     4201 CCTGGATGGC GAGATTGTCA TTGCCCCTCA CCACCAATTA CCGAGACAAC TTGTCTTCCC 
     4261 CCGATGCTAC AACGTCAGAG GAACCGAGAA CCTTCCAGAC ATTCGGCAAG ACAGTTGGAC 
     4321 CGGGACCCGA GCTGAGCCCA ACAGGCACGC GCCTGGCCAG CACTTTCGTC TCGGAGATGA 
     4381 GCTCTCTGCT GGAAATGTTG TTGGGGCAGC ACACGGTACC AGTGGAAGCT GCGTCCGCGG 
     4441 CTTTGCGGAG GCTCTCGGTG TGCGGGAGGA CCCTCAGTCT AGACCTAGCC ACCAGTGGGG 
     4501 CCTCAGCTTC AGAAGCACAG GGTAGAAAGA AGGCAGCTGA GAGCAGACTT GGCTGTGGCA 
     4561 GGAATCTAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4621 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5401 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5461 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5521 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5581 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5641 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5761 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5821 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5881 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5941 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6061 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6121 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6181 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6241 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6301 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6361 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6421 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6481 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6541 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6601 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6661 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6721 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6781 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6841 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6901 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6961 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7021 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7081 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7141 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7201 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7261 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7321 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7381 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7441 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7501 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7561 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7621 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7801 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7861 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7921 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7981 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8401 CAAAAATTTA ACGCGAATTT T
//