LOCUS       MR223027                7956 bp    DNA     circular  SYN 
DEFINITION  Col6a1 (Myc-DDK-tagged) - Mouse collagen, type VI, alpha 1 (Col6a1)
ACCESSION   NM_009933
VERSION     NM_009933.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4113..4204 
                     /label="Myc-DDK"  
     misc_feature    4234..4819 
                     /label="PolyA signal"  
     misc_feature    4291..4310 
                     /label="Restriction Enzyme"  
     misc_feature    5095..5767 
                     /label="ColE1"  
     misc_feature    6197..6991 
                     /label="Kan/Neo"  
     misc_feature    7026..7328 
                     /label="SV40 ori"  
     misc_feature    7414..7891 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4095..4101 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4095 
                     /label="Col6a1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCTGGCC CACGCTCTGC TGCCCCTGCT GCTACAAGCC TGCTGGGTGG 
     1081 CCACACAGGA CATCCAGGGC TCCAAAGCGA TTGCCTTCCA AGACTGCCCT GTGGATCTAT 
     1141 TCTTCGTGCT CGACACCTCG GAGAGTGTGG CCTTGAGGCT GAAACCTTAT GGGGCCTTGG 
     1201 TGGACAAGGT GAAGTCCTTC ACTAAGCGCT TCATTGACAA CCTGAGAGAC AGGTACTACC 
     1261 GGTGTGACCG CAACCTGGTT TGGAATGCGG GTGCGCTGCA CTACAGTGAC GAGGTGGAGA 
     1321 TCATCCGAGG GCTCACGCGC ATGCCCAGTG GCCGCGATGA GCTCAAGGCC AGCGTGGATG 
     1381 CGGTCAAGTA CTTCGGGAAA GGCACCTACA CCGACTGCGC CATTAAGAAG GGGCTGGAGG 
     1441 AGCTGCTCAT AGGGGGCTCC CACCTGAAGG AGAACAAGTA CTTGATCGTG GTGACCGACG 
     1501 GGCATCCTCT AGAGGGCTAC AAGGAACCAT GCGGGGGTCT GGAAGATGCA GTAAATGAGG 
     1561 CCAAACACCT GGGCATCAAG GTCTTTTCTG TGGCCATCAC ACCTGACCAC CTGGAGCCAC 
     1621 GTCTAAGTAT CATTGCCACA GACCACACAT ACCGGCGCAA TTTCACGGCA GCTGACTGGG 
     1681 GGCATAGCCG CGATGCAGAA GAGGTCATCA GCCAGACCAT TGACACCATT GTGGACATGA 
     1741 TTAAAAATAA CGTGGAACAA GTGTGTTGTT CTTTTGAGTG CCAGGCTGCC AGAGGACCTC 
     1801 CAGGGCCCCG AGGCGACCCT GGGTATGAGG GGGAGCGAGG AAAGCCAGGT CTTCCGGGAG 
     1861 AGAAGGGAGA AGCTGGAGAC CCTGGACGAC CTGGGGATCT TGGACCAGTC GGGTACCAGG 
     1921 GTATGAAGGG AGAAAAGGGG AGCCGTGGAG AGAAGGGTTC CAGAGGACCG AAAGGTTACA 
     1981 AGGGCGAGAA AGGCAAGCGC GGAATCGACG GGGTCGACGG CATGAAGGGA GAGACGGGGT 
     2041 ACCCAGGACT ACCGGGCTGC AAGGGCTCCC CAGGATTTGA TGGCATTCAA GGACCCCCGG 
     2101 GTCCCAAGGG TGATGCTGGT GCCTTTGGGA TGAAGGGAGA AAAGGGTGAA GCTGGAGCAG 
     2161 ACGGTGAGGC TGGGAGACCA GGGAACTCAG GGTCACCTGG AGATGAGGGT GATCCTGGAG 
     2221 AGCCTGGTCC CCCCGGAGAA AAAGGAGAGG CCGGTGATGA AGGAAATGCT GGCCCAGACG 
     2281 GTGCCCCTGG AGAGAGGGGT GGCCCTGGTG AAAGAGGACC TCGGGGGACC CCTGGTGTGA 
     2341 GAGGACCAAG GGGAGACCCG GGTGAAGCTG GACCACAGGG TGACCAAGGA AGAGAGGGGC 
     2401 CCGTCGGCAT CCCTGGAGAC TCGGGTGAGG CTGGCCCCAT TGGACCTAAA GGATACCGAG 
     2461 GTGATGAGGG TCCTCCAGGT CCTGAGGGCC TCAGAGGAGC CCCAGGACCT GTTGGTCCTC 
     2521 CTGGAGACCC CGGACTGATG GGTGAGAGAG GTGAGGATGG ACCACCAGGA AACGGCACGG 
     2581 AAGGTTTCCC CGGCTTCCCT GGGTATCCAG GCAACAGAGG CCCTCCTGGG CTAAATGGCA 
     2641 CAAAAGGCTA CCCTGGCCTC AAGGGGGATG AGGGTGAAGT GGGAGACCCA GGAGAGGATA 
     2701 ACAACGACAT TTCACCCCGT GGGGTCAAAG GGGCAAAGGG ATACCGAGGC CCAGAAGGAC 
     2761 CCCAGGGACC TCCAGGACAT GTGGGACCAC CTGGGCCAGA TGAGTGTGAG ATCCTGGATA 
     2821 TCATCATGAA AATGTGCTCC TGCTGTGAGT GCACATGTGG ACCCATTGAC ATCCTCTTCG 
     2881 TGCTGGACAG CTCGGAGAGC ATTGGCCTAC AGAACTTTGA GATTGCCAAG GACTTCATCA 
     2941 TCAAGGTCAT TGACCGGTTG AGCAAGGATG AGCTGGTCAA ATTTGAGCCA GGGCAGTCTC 
     3001 ACGCGGGCGT GGTACAGTAC AGCCACAACC AGATGCAAGA GCACGTGGAC ATGCGGAGCC 
     3061 CCAACGTCCG CAACGCCCAG GACTTCAAAG AAGCTGTCAA GAAGCTACAA TGGATGGCTG 
     3121 GTGGCACATT CACCGGAGAA GCGCTGCAGT ACACCCGGGA CCGGCTACTC CCACCCACAC 
     3181 AGAACAACCG AATTGCCCTG GTCATTACGG ATGGACGTTC TGACACTCAA CGGGACACGA 
     3241 CACCTCTCAG TGTGCTCTGT GGTGCAGACA TTCAGGTAGT TTCTGTGGGA ATCAAGGATG 
     3301 TGTTTGGCTT TGTGGCGGGC TCCGACCAGC TCAATGTCAT TTCCTGCCAA GGCTTATCGC 
     3361 AAGGTCGGCC AGGTATCTCC CTGGTGAAGG AGAACTATGC AGAGCTTCTC GATGACGGCT 
     3421 TTCTGAAGAA CATAACAGCC CAGATCTGTA TAGATAAGAA GTGTCCGGAT TATACCTGTC 
     3481 CAATCACATT CTCCTCCCCG GCTGACATCA CCATCCTGCT AGACAGCTCA GCCAGTGTCG 
     3541 GCAGCCACAA CTTCGAAACC ACCAAGGTCT TCGCCAAGCG CCTAGCTGAG CGATTCCTGT 
     3601 CAGCAGGCAG GGCGGATCCT TCCCAGGATG TGCGGGTGGC CGTGGTACAG TATAGTGGCC 
     3661 AGGGGCAGCA ACAGCCAGGT CGGGCGGCTC TTCAGTTCTT ACAGAATTAC ACAGTGCTGG 
     3721 CCAGCTCTGT GGACAGCATG GATTTCATCA ACGACGCCAC AGACGTCAAC GATGCTCTGA 
     3781 GCTACGTGAC TCGTTTCTAC CGGGAAGCCT CGTCAGGTGC CACCAAGAAG AGAGTGCTGT 
     3841 TGTTTTCAGA CGGCAACTCT CAGGGGGCCA CAGCAGAGGC CATTGAGAAG GCTGTGCAGG 
     3901 AGGCCCAGCG TGCAGGCATT GAGATCTTTG TGGTGGTGGT GGGACCCCAG GTGAACGAGC 
     3961 CCCACATCCG TGTGCTTGTC ACTGGCAAGA CTGCAGAGTA CGACGTGGCC TTTGGCGAGC 
     4021 GCCACCTATT CCGTGTACCA AACTACCAGG CCCTGCTACG TGGCGTACTC TACCAGACAG 
     4081 TCTCCAGGAA GGTGGCACTG GGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4141 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4201 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4261 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4321 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4381 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4441 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4501 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4561 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4621 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4681 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4741 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4801 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4861 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5761 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5821 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5881 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5941 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6001 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6061 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6121 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6181 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6241 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6301 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6361 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6421 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6481 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6541 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6601 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6661 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6721 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6781 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6841 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6901 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6961 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7021 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7081 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7141 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7201 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7381 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7501 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7561 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7621 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7681 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7741 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7801 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7861 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7921 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//