LOCUS       MR223023L1                8787 bp    DNA     circular  SYN 
DEFINITION  Braf (Myc-DDK-tagged) - Mouse Braf transforming gene (Braf)
ACCESSION   NM_139294
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7078..7258 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7986..8645 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7417..7985 
                     /label="ColE1"  
     misc_feature    5953..5973 
                     /label="LR50 primer"  
     misc_feature    5750..5821 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5962..6553 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5732..5738 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5732 
                     /label="Braf"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGCGCTG AGTGGCGGCG GTGGCAGCAG 
     3361 CAGCGGTGGC GGCGGCGGCG GTGGCGGCGG CGGTGGCGGT GGCGACGGCG GCGGCGGCGC 
     3421 CGAGCAGGGC CAGGCTCTGT TCAATGGCGA CATGGAGCCG GAGGCCGGCG CTGGCGCCGC 
     3481 GGCCTCTTCG GCTGCGGACC CGGCCATTCC TGAAGAGGTA TGGAATATCA AGCAAATGAT 
     3541 TAAGTTGACA CAGGAACATA TAGAGGCCCT ATTGGACAAA TTTGGTGGAG AGCATAACCC 
     3601 ACCATCAATA TACCTGGAGG CCTATGAAGA GTACACCAGC AAGCTAGATG CCCTTCAGCA 
     3661 AAGAGAACAG CAGCTTTTGG AATCCCTGGT TTTTCAAACT CCCACAGATG CATCACGGAA 
     3721 CAACCCCAAG TCACCACAGA AACCTATCGT TAGAGTCTTC CTGCCCAACA AACAGAGGAC 
     3781 AGTGGTACCC GCAAGATGTG GTGTTACAGT TCGAGACAGT CTAAAGAAAG CACTGATGAT 
     3841 GAGAGGTCTC ATCCCAGAAT GCTGTGCTGT TTACAGAATT CAGGATGGAG AGAAGAAACC 
     3901 AATTGGCTGG GACACGGACA TTTCCTGGCT TACTGGAGAG GAGTTACATG TTGAAGTACT 
     3961 GGAGAATGTC CCACTTACAA CACACAACTT TGTACGGAAA ACTTTTTTCA CCTTAGCATT 
     4021 TTGTGACTTT TGCCGAAAGC TGCTTTTCCA GGGTTTCCGT TGTCAAACAT GTGGTTATAA 
     4081 ATTTCACCAG CGTTGTAGTA CAGAGGTTCC ACTGATGTGT GTAAATTATG ACCAACTTGA 
     4141 TTTGCTGTTT GTCTCCAAGT TCTTTGAGCA TCACCCAGTA CCACAGGAGG AGGCCTCCTT 
     4201 CCCAGAGACT GCCCTTCCAT CTGGATCCTC TTCCGCACCC CCCTCAGACT CTACTGGGCC 
     4261 CCAAATCCTC ACCAGTCCAT CTCCTTCAAA ATCCATTCCA ATTCCACAGC CCTTCCGACC 
     4321 AGCAGATGAA GATCATCGCA ATCAGTTTGG GCAACGAGAC CGGTCCTCCT CAGCTCCCAA 
     4381 TGTTCATATA AACACAATTG AGCCTGTGAA TATCGATGAA AAATTCCCAG AAGTGGAATT 
     4441 ACAGGATCAA AGGGATTTGA TTAGAGACCA GGGGTTTCGT GGTGATGGAG CCCCCTTGAA 
     4501 CCAACTGATG CGCTGTCTTC GGAAATACCA ATCCCGGACT CCCAGCCCCC TCCTCCATTC 
     4561 TGTCCCCAGT GAAATAGTGT TTGATTTTGA GCCTGGCCCA GTGTTCAGAG GGTCAACCAC 
     4621 AGGCTTGTCC GCCACCCCGC CTGCCTCATT ACCTGGCTCA CTCACTAACG TGAAAGCCTT 
     4681 ACAGAAATCT CCAGGTCCTC AGCGGGAAAG GAAGTCATCT TCTTCCTCAT CCTCGGAGGA 
     4741 CAGAAGTCGG ATGAAAACAC TTGGTAGAAG AGATTCAAGT GATGACTGGG AGATTCCTGA 
     4801 TGGACAGATT ACAGTGGGAC AGAGAATTGG ATCTGGGTCA TTTGGAACTG TCTACAAGGG 
     4861 AAAGTGGCAT GGTGATGTGG CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAACA 
     4921 GCTACAGGCC TTCAAAAATG AAGTAGGAGT GCTCAGGAAA ACTCGACATG TGAATATCCT 
     4981 CCTTTTCATG GGCTATTCTA CAAAGCCACA ACTGGCAATT GTTACACAGT GGTGTGAGGG 
     5041 CTCCAGCTTA TATCACCATC TCCACATCAT TGAGACCAAA TTTGAGATGA TCAAACTTAT 
     5101 AGATATTGCT CGGCAGACTG CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA 
     5161 CAGAGACCTC AAGAGTAATA ATATATTTCT TCATGAAGAC CTCACGGTAA AAATAGGTGA 
     5221 CTTTGGTCTA GCCACAGTGA AATCTCGGTG GAGTGGGTCC CATCAGTTTG AACAGTTGTC 
     5281 TGGATCTATT TTGTGGATGG CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCGTATAG 
     5341 CTTTCAGTCA GACGTGTATG CGTTTGGGAT TGTTCTGTAC GAACTGATGA CCGGCCAGCT 
     5401 ACCTTATTCA AACATCAACA ACAGGGATCA GATAATTTTT ATGGTGGGAC GAGGATACCT 
     5461 ATCTCCAGAT CTCAGTAAGG TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC 
     5521 AGAGTGCCTC AAAAAGAAAA GAGACGAGAG ACCACTCTTT CCCCAAATTC TCGCCTCCAT 
     5581 TGAGCTGCTG GCCCGCTCAT TGCCAAAAAT TCACCGCAGT GCATCAGAAC CTTCCTTGAA 
     5641 TCGGGCTGGT TTCCAAACAG AAGATTTTAG TCTGTATGCT TGTGCTTCTC CGAAAACACC 
     5701 CATCCAAGCA GGGGGATATG GAGAATTTGC AGCCTTCAAG ACGCGTACGC GGCCGCTCGA 
     5761 GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA 
     5821 CGACGATAAG GTTTAAACGG CCGGCCGCGG TCTGTACAAG TAGGATTCGT CGAGGGACCT 
     5881 AATAACTTCG TATAGCATAC ATTATACGAA GTTATACATG TTTAAGGGTT CCGGTTCCAC 
     5941 TAGGTACAAT TCGATATCAA GCTTATCGAT AATCAACCTC TGGATTACAA AATTTGTGAA 
     6001 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA 
     6061 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA 
     6121 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG 
     6181 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC 
     6241 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC 
     6301 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG 
     6361 GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG 
     6421 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG 
     6481 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC 
     6541 CTTTGGGCCG CCTCCCCGCA TCGATACCGT CGACCTCGAT CGAGACCTAG AAAAACATGG 
     6601 AGCAATCACA AGTAGCAATA CAGCAGCTAC CAATGCTGAT TGTGCCTGGC TAGAAGCACA 
     6661 AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC ACCTCAGGTA CCTTTAAGAC CAATGACTTA 
     6721 CAAGGCAGCT GTAGATCTTA GCCACTTTTT AAAAGAAAAG GGGGGACTGG AAGGGCTAAT 
     6781 TCACTCCCAA CGAAGACAAG ATATCCTTGA TCTGTGGATC TACCACACAC AAGGCTACTT 
     6841 CCCTGATTGG CAGAACTACA CACCAGGGCC AGGGATCAGA TATCCACTGA CCTTTGGATG 
     6901 GTGCTACAAG CTAGTACCAG TTGAGCAAGA GAAGGTAGAA GAAGCCAATG AAGGAGAGAA 
     6961 CACCCGCTTG TTACACCCTG TGAGCCTGCA TGGGATGGAT GACCCGGAGA GAGAAGTATT 
     7021 AGAGTGGAGG TTTGACAGCC GCCTAGCATT TCATCACATG GCCCGAGAGC TGCATCCGGA 
     7081 CTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG 
     7141 GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG 
     7201 TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT 
     7261 CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     7321 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     7381 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     7441 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     7501 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     7561 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     7621 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     7681 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     7741 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     7801 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     7861 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     7921 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     7981 GATTTTGGTC ATGATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA 
     8041 AGCATTCTGC CGACATGGAA GCCATCACAA ACGGCATGAT GAACCTGAAT CGCCAGCGGC 
     8101 ATCAGCACCT TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG 
     8161 TTGTCCATAT TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG 
     8221 ACGAAAAACA TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC 
     8281 GCCACATCTT GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG 
     8341 AGCGATGAAA ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC 
     8401 CATATCACCA GCTCACCGTC TTTCATTGCC ATACGGAACT CCGGATGAGC ATTCATCAGG 
     8461 CGGGCAAGAA TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT 
     8521 AAAAAGGCCG TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA 
     8581 AATGCCTCAA AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG 
     8641 ATTTTTTTCT CCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8701 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTCCCGCGC 
     8761 ACATTTCCCC GAAAAGTGCC ACCTGAC
//