LOCUS       MR223023                7293 bp    DNA     circular  SYN 
DEFINITION  Braf (Myc-DDK-tagged) - Mouse Braf transforming gene (Braf)
ACCESSION   NM_139294
VERSION     NM_139294.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3450..3541 
                     /label="Myc-DDK"  
     misc_feature    3571..4156 
                     /label="PolyA signal"  
     misc_feature    3628..3647 
                     /label="Restriction Enzyme"  
     misc_feature    4432..5104 
                     /label="ColE1"  
     misc_feature    5534..6328 
                     /label="Kan/Neo"  
     misc_feature    6363..6665 
                     /label="SV40 ori"  
     misc_feature    6751..7228 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3432..3438 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3432 
                     /label="Braf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGCTG AGTGGCGGCG GTGGCAGCAG CAGCGGTGGC GGCGGCGGCG 
     1081 GTGGCGGCGG CGGTGGCGGT GGCGACGGCG GCGGCGGCGC CGAGCAGGGC CAGGCTCTGT 
     1141 TCAATGGCGA CATGGAGCCG GAGGCCGGCG CTGGCGCCGC GGCCTCTTCG GCTGCGGACC 
     1201 CGGCCATTCC TGAAGAGGTA TGGAATATCA AGCAAATGAT TAAGTTGACA CAGGAACATA 
     1261 TAGAGGCCCT ATTGGACAAA TTTGGTGGAG AGCATAACCC ACCATCAATA TACCTGGAGG 
     1321 CCTATGAAGA GTACACCAGC AAGCTAGATG CCCTTCAGCA AAGAGAACAG CAGCTTTTGG 
     1381 AATCCCTGGT TTTTCAAACT CCCACAGATG CATCACGGAA CAACCCCAAG TCACCACAGA 
     1441 AACCTATCGT TAGAGTCTTC CTGCCCAACA AACAGAGGAC AGTGGTACCC GCAAGATGTG 
     1501 GTGTTACAGT TCGAGACAGT CTAAAGAAAG CACTGATGAT GAGAGGTCTC ATCCCAGAAT 
     1561 GCTGTGCTGT TTACAGAATT CAGGATGGAG AGAAGAAACC AATTGGCTGG GACACGGACA 
     1621 TTTCCTGGCT TACTGGAGAG GAGTTACATG TTGAAGTACT GGAGAATGTC CCACTTACAA 
     1681 CACACAACTT TGTACGGAAA ACTTTTTTCA CCTTAGCATT TTGTGACTTT TGCCGAAAGC 
     1741 TGCTTTTCCA GGGTTTCCGT TGTCAAACAT GTGGTTATAA ATTTCACCAG CGTTGTAGTA 
     1801 CAGAGGTTCC ACTGATGTGT GTAAATTATG ACCAACTTGA TTTGCTGTTT GTCTCCAAGT 
     1861 TCTTTGAGCA TCACCCAGTA CCACAGGAGG AGGCCTCCTT CCCAGAGACT GCCCTTCCAT 
     1921 CTGGATCCTC TTCCGCACCC CCCTCAGACT CTACTGGGCC CCAAATCCTC ACCAGTCCAT 
     1981 CTCCTTCAAA ATCCATTCCA ATTCCACAGC CCTTCCGACC AGCAGATGAA GATCATCGCA 
     2041 ATCAGTTTGG GCAACGAGAC CGGTCCTCCT CAGCTCCCAA TGTTCATATA AACACAATTG 
     2101 AGCCTGTGAA TATCGATGAA AAATTCCCAG AAGTGGAATT ACAGGATCAA AGGGATTTGA 
     2161 TTAGAGACCA GGGGTTTCGT GGTGATGGAG CCCCCTTGAA CCAACTGATG CGCTGTCTTC 
     2221 GGAAATACCA ATCCCGGACT CCCAGCCCCC TCCTCCATTC TGTCCCCAGT GAAATAGTGT 
     2281 TTGATTTTGA GCCTGGCCCA GTGTTCAGAG GGTCAACCAC AGGCTTGTCC GCCACCCCGC 
     2341 CTGCCTCATT ACCTGGCTCA CTCACTAACG TGAAAGCCTT ACAGAAATCT CCAGGTCCTC 
     2401 AGCGGGAAAG GAAGTCATCT TCTTCCTCAT CCTCGGAGGA CAGAAGTCGG ATGAAAACAC 
     2461 TTGGTAGAAG AGATTCAAGT GATGACTGGG AGATTCCTGA TGGACAGATT ACAGTGGGAC 
     2521 AGAGAATTGG ATCTGGGTCA TTTGGAACTG TCTACAAGGG AAAGTGGCAT GGTGATGTGG 
     2581 CAGTGAAAAT GTTGAATGTG ACAGCACCCA CACCTCAACA GCTACAGGCC TTCAAAAATG 
     2641 AAGTAGGAGT GCTCAGGAAA ACTCGACATG TGAATATCCT CCTTTTCATG GGCTATTCTA 
     2701 CAAAGCCACA ACTGGCAATT GTTACACAGT GGTGTGAGGG CTCCAGCTTA TATCACCATC 
     2761 TCCACATCAT TGAGACCAAA TTTGAGATGA TCAAACTTAT AGATATTGCT CGGCAGACTG 
     2821 CACAGGGCAT GGATTACTTA CACGCCAAGT CAATCATCCA CAGAGACCTC AAGAGTAATA 
     2881 ATATATTTCT TCATGAAGAC CTCACGGTAA AAATAGGTGA CTTTGGTCTA GCCACAGTGA 
     2941 AATCTCGGTG GAGTGGGTCC CATCAGTTTG AACAGTTGTC TGGATCTATT TTGTGGATGG 
     3001 CACCAGAAGT AATCAGAATG CAAGATAAAA ACCCGTATAG CTTTCAGTCA GACGTGTATG 
     3061 CGTTTGGGAT TGTTCTGTAC GAACTGATGA CCGGCCAGCT ACCTTATTCA AACATCAACA 
     3121 ACAGGGATCA GATAATTTTT ATGGTGGGAC GAGGATACCT ATCTCCAGAT CTCAGTAAGG 
     3181 TACGGAGTAA CTGTCCAAAA GCCATGAAGA GATTAATGGC AGAGTGCCTC AAAAAGAAAA 
     3241 GAGACGAGAG ACCACTCTTT CCCCAAATTC TCGCCTCCAT TGAGCTGCTG GCCCGCTCAT 
     3301 TGCCAAAAAT TCACCGCAGT GCATCAGAAC CTTCCTTGAA TCGGGCTGGT TTCCAAACAG 
     3361 AAGATTTTAG TCTGTATGCT TGTGCTTCTC CGAAAACACC CATCCAAGCA GGGGGATATG 
     3421 GAGAATTTGC AGCCTTCAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3481 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     3541 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     3601 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     3661 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     3721 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     3781 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     3841 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     3901 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     3961 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4021 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4081 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4141 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4201 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4261 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4321 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4381 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4441 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4501 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     4561 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     4621 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     4681 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     4741 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     4801 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     4861 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     4921 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     4981 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5041 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5101 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5161 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5221 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5281 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5341 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5401 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5461 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     5521 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     5581 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     5641 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     5701 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     5761 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     5821 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     5881 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     5941 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6001 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6061 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6121 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6181 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6241 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6301 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6361 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6421 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6481 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     6541 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6601 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6661 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     6721 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     6781 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     6841 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     6901 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     6961 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7021 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7081 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7141 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7201 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7261 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//