LOCUS       MR222994                9045 bp    DNA     circular  SYN 
DEFINITION  Rock2 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2)
ACCESSION   NM_009072
VERSION     NM_009072.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5202..5293 
                     /label="Myc-DDK"  
     misc_feature    5323..5908 
                     /label="PolyA signal"  
     misc_feature    5380..5399 
                     /label="Restriction Enzyme"  
     misc_feature    6184..6856 
                     /label="ColE1"  
     misc_feature    7286..8080 
                     /label="Kan/Neo"  
     misc_feature    8115..8417 
                     /label="SV40 ori"  
     misc_feature    8503..8980 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5184..5190 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5184 
                     /label="Rock2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCGGCCC CCGCCGACGG GGAAAATGCC CGGCGCCCCC GAGACCGCGC 
     1081 CGGGGGACGG GGCGGGCGCC GGCCGTCAGA GGAAGCTGGA GGCGCTCATC CGAGACCCTC 
     1141 GCTCGCCCAT CAACGTGGAG AGTCTGCTGG ATGGCTTAAA TTCCTTGGTC CTTGATTTGG 
     1201 ATTTTCCTGC TTTGAGGAAA AATAAAAATA TAGATAACTT CTTAAATAGA TATGAGAAAA 
     1261 TTGTGAAAAA AATCAGAGGT TTACAGATGA AAGCGGAAGA CTATGATGTT GTAAAAGTTA 
     1321 TTGGAAGAGG TGCTTTTGGT GAAGTTCAGT TGGTTCGTCA TAAGGCATCA CAGAAGGTTT 
     1381 ATGCAATGAA GCTTCTTAGT AAATTTGAAA TGATAAAAAG ATCAGATTCT GCTTTTTTCT 
     1441 GGGAAGAAAG AGATATTATG GCCTTTGCCA ACAGTCCCTG GGTAGTTCAG CTCTTCTGTG 
     1501 CCTTTCAAGA TGACAGGTAC CTGTACATGG TGATGGAGTA CATGCCAGGC GGAGACCTCG 
     1561 TCAACCTTAT GAGTAATTAT GATGTACCTG AAAAGTGGGC CAAGTTTTAC ACTGCTGAAG 
     1621 TAGTGCTTGC ACTGGATGCA ATACACTCCA TGGGCTTAAT ACACAGAGAT GTGAAGCCTG 
     1681 ATAACATGCT ATTGGATAAA CATGGACATC TAAAGTTAGC AGATTTTGGC ACATGTATGA 
     1741 AAATGGATGA AACAGGCATG GTGCATTGCG ACACAGCAGT TGGAACTCCC GACTACATAT 
     1801 CGCCCGAGGT TCTGAAATCA CAAGGAGGTG ATGGTTACTA TGGGCGAGAA TGTGATTGGT 
     1861 GGTCTGTAGG TGTTTTCCTT TTTGAGATGT TAGTAGGGGA CACTCCATTT TACGCAGATT 
     1921 CACTTGTAGG AACCTACAGC AAAATTATGG ATCATAAAAA TTCACTGTGT TTTCCTGAAG 
     1981 ATACAGAAAT TTCTAAACAT GCGAAGAATC TCATATGTGC CTTCTTAACA GACAGGGAGG 
     2041 TACGACTTGG AAGAAATGGG GTAGAAGAAA TCAAACAGCA TCCTTTCTTT AAGAATGATC 
     2101 AATGGAATTG GGATAACATA AGAGAGACGG CAGCTCCAGT GGTACCTGAG CTCAGCAGTG 
     2161 ACATAGACAG CAGCAACTTT GACGACATTG AGGATGACAA AGGCGATGTA GAGACCTTCC 
     2221 CGATTCCTAA AGCTTTTGTG GGAAATCAGC TGCCTTTTAT AGGATTTACC TACTTTAGAG 
     2281 AAAATTTGTT ATTAAGTGAC TCTCCACCTT GTAGAGAAAA TGATGCAATA CAAACAAGGA 
     2341 AAAGTGAAGA AAGTCAGGAG ATTCAGAAAA AATTATATGC ACTAGAAGAA CACCTTAGCA 
     2401 GTGAGGTGCA AGCCAAGGAG GAGCTGGAAC AGAAGTGCAA ATCTATTAAT ACTCGCCTAG 
     2461 AGAAAACAGC AAAGGAGCTA GAAGAAGAGA TTACCTTACG GAAAAGTGTG GAATCAACAT 
     2521 TAAGACAGCT GGAAAGAGAA AAGGCCCTTC TTCAGCACAA AAATGCAGAG TATCAGCGGA 
     2581 AAGCTGATCA TGAAGCCGAC AAGAAGCGGA ACTTGGAAAA TGATGTTAAC AGCTTAAAAG 
     2641 ATCAACTTGA AGATCTGAAG AAAAGGAACC AGAGCTCTCA GATATCCACT GAGAAGGTCA 
     2701 ATCAGCTCCA GAAACAACTG GATGAAGCTA ATGCTTTGCT GAGAACAGAG TCTGATACTG 
     2761 CAGCACGGTT AAGAAAAACC CAGGCAGAAA GTTCCAAACA GATTCAGCAG CTGGAATCTA 
     2821 ACAATAGAGA TCTACAAGAT AAAAACTGCC TGCTTGAGAC TGCCAAGTTA AAACTTGAAA 
     2881 AGGAATTTAT CAATCTTCAG TCAGCTTTAG AATCTGAAAG AAGGGACCGG ACCCATGGAT 
     2941 CAGAGATAAT TAATGATTTA CAAGGTAGAA TATCTGGGTT GGAAGAAGAT TTGAAGACTG 
     3001 GCAAAGCCTT ACTAGCAAAA GTAGAGCTGG AGAAGAGACA GCTGCAGGAG AAGCTCACTG 
     3061 ACTTAGAGAA GGAAAAAAGC AACATGGAAA TAGATATGAC ATATCAACTG AAAGTTATAC 
     3121 AGCAGAGTTT AGAACAAGAA GAAGCTGAAC ACAAGACCAC AAAAGCACGG CTAGCGGATA 
     3181 AAAATAAGAT CTATGAATCC ATTGAAGAAG CTAAATCAGA AGCCATGAAA GAAATGGAGA 
     3241 AGAAGCTGTT GGAAGAGAGG TCTTTAAAGC AGAAAGTGGA GAACCTGCTC CTGGAAGCCG 
     3301 AGAAAAGATG CTCTATATTG GACTGTGACC TCAAACAGTC TCAGCAGAAG CTAAATGAGC 
     3361 TGCTGAAGCA GAAAGATGTG CTGAACGAGG ATGTTAGAAA TTTGACATTA AAAATAGAGC 
     3421 AGGAAACTCA GAAGCGTTGT CTTATGCAAA ATGACCTGAA GATGCAGACC CAACAAGTTA 
     3481 ACACCCTAAA AATGTCAGAA AAGCAAATAA AACAAGAAAA TAATCATCTT ATGGAAATGA 
     3541 AAATGAACTT GGAAAAGCAA AATACTGAAC TTCGAAAAGA GCGGCAGGAT GCAGATGGGC 
     3601 AGATGAAGGA ACTGCAAGAC CAACTTGAAG CAGAGCAGTA TTTCTCAACC CTCTATAAAA 
     3661 CACAAGTTAG AGAACTTAAA GAAGAAAATG AAGAGAAGAC AAAACTTTGT AAAGAGTTGC 
     3721 AACAGAAGAA GCAGGATTTA CAGGATGAAA GAGACTCTTT GGCTGCCCAG CTGGAAATTA 
     3781 CCCTTACCAA AGCAGATTCT GAGCAGCTGG CTCGTTCCAT TGCTGAAGAA CAATACTCTG 
     3841 ATTTGGAAAA AGAGAAGATC ATGAAAGAGC TGGAGATCAA AGAGATGATG GCTAGACACA 
     3901 AACAAGAACT TACTGAAAAG GATACTACAA TTGCATCTCT TGAAGAAACA AATAGGACAC 
     3961 TAACTAGCGA TGTTGCAAAT CTTGCAAATG AGAAAGAAGA ATTAAACAAC AAGCTGAAAG 
     4021 ATAGCCAAGA ACAACTGTCC AAGTTGAAAG ATGAAGAGAT GAGTGCAGCA GCTATTAAAG 
     4081 CACAGTTTGA GAAGCAGCTT CTGAATGAGA GAACTCTCAA GACTCAAGCT GTGAATAAGT 
     4141 TGGCGGAGAT CATGAATCGA AAAGAACCTG TCAAGCGTGG TAGTGACACA GATGTGCGAA 
     4201 GAAAAGAGAA GGAAAACAGA AAGCTACATA TGGAGCTTAA GTCTGAACGT GAAAAATTGA 
     4261 CACAACAGAT GATCAAATAT CAGAAAGAAC TGAATGAAAT GCAGGCTCAA ATAGCTGAAG 
     4321 AGAGCCAAAT TCGAATAGAA CTCCAGATGA CCCTGGACAG TAAAGACAGC GACATTGAAC 
     4381 AGCTGCGGTC ACAGCTCCAG GCCTTGCATA TTGGTATGGA CAGTTCCAGT ATAGGCAGTG 
     4441 GACCAGGCGA TGCTGAGCCT GATGATGGAT TTCCAGAATC AAGATTAGAA GGATGGTTGT 
     4501 CATTGCCTGT GCGCAACAAC ACTAAAAAGT TTGGATGGGT TAAAAAGTAT GTGATTGTAA 
     4561 GCAGCAAGAA GATTCTTTTT TATGACAGCG AACAAGATAA AGAGCAGTCC AACCCTTACA 
     4621 TGGTGTTAGA CATAGACAAG CTATTTCACG TCCGACCTGT TACCCAGACA GATGTATACA 
     4681 GAGCAGATGC TAAAGAAATT CCAAGGATAT TTCAGATTCT ATATGCCAAT GAAGGAGAAA 
     4741 GTAAGAAGGA ACCAGAGTTT CCAGTGGAAC CAGTGGGAGA AAAATCTAAT TATATTTGTC 
     4801 ACAAAGGACA TGAGTTTATT CCTACTCTTT ATCACTTCCC AACCAACTGT GAGGCATGTA 
     4861 TGAAGCCGCT GTGGCACATG TTTAAGCCTC CTCCTGCTCT AGAGTGCCGT AGGTGCCACA 
     4921 TTAAATGTCA TAAAGATCAC ATGGACAAGA AGGAAGAGAT TATAGCGCCC TGCAAAGTTT 
     4981 ATTATGATAT ATCGTCGGCA AAGAATCTGT TGTTATTAGC AAATTCAACA GAAGAACAGC 
     5041 AGAAATGGGT TAGTCGGTTG GTGAAAAAGA TACCTAAAAA GCCTCCAGCT CCAGACCCTT 
     5101 TTGCCCGATC ATCCCCTAGA ACTTCAATGA AAATACAACA AAACCAGTCC ATTCGGCGGC 
     5161 CCAGTCGACA GCTTGCTCCA AACAAGCCAA GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5221 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5281 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5341 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5401 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5461 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5521 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5581 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5641 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5701 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5761 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5821 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5881 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5941 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6001 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6061 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6121 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6181 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6241 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6301 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6361 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6421 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6481 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6541 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6601 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6661 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6721 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6781 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6841 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6901 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6961 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7021 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7081 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7141 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7201 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7261 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7321 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7381 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7441 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7501 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7561 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7621 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7681 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7741 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7801 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7861 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7921 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7981 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8041 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8101 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8161 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8221 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8281 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8341 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8401 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8461 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8521 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8581 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8641 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8701 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8761 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8821 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8881 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8941 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9001 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//