LOCUS       MR222942                8643 bp    DNA     circular  SYN 
DEFINITION  Cyfip1 (Myc-DDK-tagged) - Mouse cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant 3
ACCESSION   NM_001164662
VERSION     NM_001164662.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4798..4889 
                     /label="Myc-DDK"  
     misc_feature    4919..5504 
                     /label="PolyA signal"  
     misc_feature    4976..4995 
                     /label="Restriction Enzyme"  
     misc_feature    5780..6452 
                     /label="ColE1"  
     misc_feature    6882..7676 
                     /label="Kan/Neo"  
     misc_feature    7711..8013 
                     /label="SV40 ori"  
     misc_feature    8099..8576 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4773..4780 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4773 
                     /label="Cyfip1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCCAA GTGACACTAG AGGACGCACT GTCCAATGTG GACCTCCTGG 
     1081 AGGAGCTGCC TCTGCCTGAC CAGCAGCCCT GCATCGAGCC CCCACCGTCC TCGCTGCTCT 
     1141 ATCAGCCAAA TTTCAACACC AACTTTGAAG ACAGAAATGC ATTTGTCACT GGCATTGCAA 
     1201 GATACATCGA ACAAGCAACT GTCCACTCTA GCATGAACGA GATGCTAGAG GAAGGCCAAG 
     1261 AGTATGCTGT CATGCTGTAC ACCTGGAGGA GCTGCTCCCG GGCCATCCCT CAGGTGAAGT 
     1321 GCAACGAGCA GCCGAATAGA GTTGAAATTT ATGAGAAAAC CGTGGAAGTC CTTGAACCCG 
     1381 AGGTCACAAA ACTGATGAAT TTTATGTACT TTCAGAGAAA TGCCATCGAG CGGTTCTGTG 
     1441 GGGAAGTGAG ACGCCTGTGT CATGCTGAGA GGAGAAAGGA CTTTGTTTCT GAAGCCTACT 
     1501 TGATCACCCT GGGCAAATTT ATCAACATGT TTGCTGTGTT GGATGAGCTG AAGAACATGA 
     1561 AGTGCAGTGT GAAGAATGAC CACTCTGCAT ATAAGAGGGC TGCTCAGTTT TTACGTAAAA 
     1621 TGGCAGATCC ACAATCCATC CAGGAGTCAC AGAATCTGTC CATGTTCCTG GCCAACCACA 
     1681 ACAAGATTAC ACAATCTCTG CAGCAGCAGC TTGAGGTCAT TTCTGGCTAT GAAGAGCTCC 
     1741 TGGCAGACAT CGTGAATCTG TGTGTGGATT ACTATGAGAA CAGGATGTAC TTGACACCCA 
     1801 GCGAAAAACA CATGCTTCTC AAAGTCATGG GATTTGGCCT GTACCTGATG GATGGGAGTG 
     1861 TGAGTAACAT CTACAAACTA GATGCCAAGA AAAGAATAAA TTTATCCAAA ATCGACAAGT 
     1921 ATTTCAAGCA GCTGCAGGTG GTTCCACTCT TTGGAGATAT GCAAATAGAA CTGGCAAGAT 
     1981 ACATCAAGAC CAGCGCACAC TATGAGGAGA ATAAGTCCCG GTGGACCTGC GCGTCCTCCA 
     2041 GCAGCAGCCC GCAGTACAAC ATCTGTGAGC AGATGATCCA GATCCGTGAG GACCATATGC 
     2101 GTTTCATCTC CGAGCTGGCA CGCTACAGCA ATAGCGAGGT AGTCACAGGG TCTGGCCGGC 
     2161 AGGAAGCCCA GAAGACAGAT GCTGAGTACC GGAAGCTCTT CGATCTGGCT CTGCAGGGCC 
     2221 TGCAGCTTCT GTCGCAGTGG AGCGCTCACG TGATGGAAGT GTATTCCTGG AAACTTGTAC 
     2281 ATCCAACAGA CAAGTACTCC AACAAGGACT GCCCCGACAA CGCTGAGGAG TACGAGCGAG 
     2341 CCACACGCTA CAACTACACC ACTGAGGAGA AGTTTGCCCT GGTGGAGGTG ATTGCCATGA 
     2401 TCAAAGGCCT GCAGGTGCTG ATGGGCAGGA TGGAGAGTGT GTTCAATCAC GCCATCAGAC 
     2461 ACACGGTTTA CGCTGCGCTT CAGGACTTCT CCCAGGTGAC CCTCAGGGAA CCACTGCGAC 
     2521 AGGCCATCAA GAAGAAAAAG AACGTAATCC AGAGTGTCCT ACAGGCCATC AGGAAGACTG 
     2581 TGTGTGACTG GGAGACTGGG CATGAGCCCT TTAACGACCC AGCTCTGCGG GGAGAGAAGG 
     2641 ACCCTAAGAG TGGCTTTGAC ATCAAGGTGC CACGCCGTGC TGTGGGACCC TCCAGCACGC 
     2701 AGCTCTACCT GGTGCGCACT ATGGCTGAGT CCTTGAGCTC TGCCGAGCTG CTGAGGCAGC 
     2761 TCAAGTCTCT GGGCATGGAA AGGCTCTTGC ATGTGGTAAA CGCGTTTCTG AGGCAGTCGT 
     2821 ACACCTATCC ACCTCTTTTA ACCTTTGGTG AAACACTGCA GCAGTGCTGT GACCTTTCCC 
     2881 AGCTGTGGTT CCGAGAGTTC TTCCTGGAGC TGACCATGGG CAGGAGGATC CAGTTCCCCA 
     2941 TTGAGATGTC TATGCCCTGG ATTCTGACTG ACCACATCCT AGAGACCAAG GAGGCATCAA 
     3001 TGATGGAGTA CGTCCTCTAC TCCTTGGACC TGTACAACGA CAGTGCCCAC TATGCGCTCA 
     3061 CCAAGTTCAA CAAGCAGTTT CTCTATGATG AGATTGAGGC AGAGGTGAAT CTGTGTTTTG 
     3121 ACCAATTTGT TTATAAACTA GCAGACCAGA TATTTGCATA TTACAAGGTT ATGGCAGGAA 
     3181 GTTTGCTTCT TGACAAAAGG TTACGGTCAG AATGCAAAAA TCAGGGAGCC ACGATCCACC 
     3241 TCCCCCCATC TAACCGCTAT GAGACGCTGT TGAAGCAAAG GCACGTGCAG CTGCTCGGGA 
     3301 GATCCATTGA TCTCAACCGT CTGATTACAC AGCGCGTTTC AGCAGCCATG TACAAATCTC 
     3361 TAGAACTGGC AATTGGACGG TTTGAAAGTG AAGATTTAAC ATCAGTAGTC GAGCTAGATG 
     3421 GACTCTTAGA AATCAACCGT ATGACACACA AGCTGTTGAG CAGGTACCTC ACACTGGACA 
     3481 GCTTTGATGC CATGTTCCGG GAAGCCAACC ACAACGTGTC TGCACCTTAT GGAAGAATTA 
     3541 CCCTCCACGT CTTCTGGGAG CTAAACTATG ACTTCTTGCC CAACTACTGC TATAATGGCT 
     3601 CTACCAACCG GTTTGTTCGG ACAGTATTAC CATTTTCTCA GGAATTTCAA AGAGACAAAC 
     3661 AGCCTAACGC ACAGCCCCAG TATTTGCATG GATCCAAGGC TTTGAACCTA GCCTACTCAA 
     3721 GCATTTATGG CAGCTACCGG AACTTTGTGG GGCCTCCGCA CTTCCAAGTC ATCTGCCGGC 
     3781 TACTTGGCTA CCAGGGCATT GCAGTAGTCA TGGAAGAGCT GCTGAAGGTT GTCAAGAGCC 
     3841 TGCTGCAAGG CACAATCCTG CAGTACGTGA AAACTCTGAT GGAAGTGATG CCCAAGATCT 
     3901 GCCGGCTTCC GCGACATGAG TACGGCTCTC CTGGCATCCT GGAGTTCTTC CACCACCAGC 
     3961 TGAAGGACAT TGTGGAGTAT GCAGAGCTGA AAACCGTATG CTTCCAGAAC CTGCGGGAGG 
     4021 TGGGCAATGC TGTTCTCTTC TGCCTGCTTA TTGAGCAAAG CCTGTCTTTA GAAGAAGTCT 
     4081 GTGACCTGTT GCATGCAGCT CCTTTCCAGA ATATCTTACC TCGAATCCAT GTAAAAGAGG 
     4141 GGGAGAGAGT TGATGCCAAA ATGAAAAGAC TAGAATCCAA GTATGCCCCA CTGCACCTTG 
     4201 TCCCACTGAT TGAAAGGCTG GGGACCCCAC AGCAAATTGC AATTGCAAGA GAGGGGGACT 
     4261 TGCTAACCAA GGAGCGTCTC TGTTGTGGTC TGTCCATGTT TGAAGTCATC CTGACACGGA 
     4321 TCCGGACCTT TCTGGATGAT CCCATCTGGC GTGGGCCCCT ACCCAGCAAT GGTGTCATGC 
     4381 ACGTGGATGA GTGTGTGGAG TTTCACAGAC TATGGAGTGC CATGCAGTTT GTTTACTGCA 
     4441 TTCCTGTAGG GACACACGAG TTTACAGTGG AGCAGTGTTT TGGAGATGGG CTCCACTGGG 
     4501 CTGGCTGCAT GATCATTGTA CTTCTTGGAC AGCAGCGGCG CTTTGCTGTG TTGGATTTCT 
     4561 GCTATCATCT TCTCAAAGTT CAGAAACATG ATGGCAAAGA TGAGATCATC AAAAATGTGC 
     4621 CATTGAAGAA GATGGTGGAG AGGATCCGCA AGTTCCAGAT TCTCAACGAT GAAATCATCA 
     4681 CTATCCTGGA CAAGTACTTG AAATCTGGTG ATGGCGAGAG CACACCTGTG GAGCATGTAC 
     4741 GCTGCTTCCA GCCACCCATC CACCAGTCCC TAGCCAGTAG CAGCGGACCG ACGCGTACGC 
     4801 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4861 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4921 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4981 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5041 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5101 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5161 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5221 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5281 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5341 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5401 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5461 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5521 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5581 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5641 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5701 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5761 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5821 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5881 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5941 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6001 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6061 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6121 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6181 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6241 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6301 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6361 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6421 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6481 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6541 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6601 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6661 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6721 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6781 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6841 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6901 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6961 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7021 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7081 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7141 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7201 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7261 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7321 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7381 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7441 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7501 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7561 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7621 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7681 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7741 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7801 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7861 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7921 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7981 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8041 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8101 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8161 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8221 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8281 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8341 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8401 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8461 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8521 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8581 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8641 TTT
//