LOCUS       MR222940                8640 bp    DNA     circular  SYN 
DEFINITION  Cyfip1 (Myc-DDK-tagged) - Mouse cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant 1
ACCESSION   NM_001164661
VERSION     NM_001164661.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4797..4888 
                     /label="Myc-DDK"  
     misc_feature    4918..5503 
                     /label="PolyA signal"  
     misc_feature    4975..4994 
                     /label="Restriction Enzyme"  
     misc_feature    5779..6451 
                     /label="ColE1"  
     misc_feature    6881..7675 
                     /label="Kan/Neo"  
     misc_feature    7710..8012 
                     /label="SV40 ori"  
     misc_feature    8098..8575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4779..4785 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4779 
                     /label="Cyfip1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGCCCAA GTGACACTAG AGGACGCACT GTCCAATGTG GACCTCCTGG 
     1081 AGGAGCTGCC TCTGCCTGAC CAGCAGCCCT GCATCGAGCC CCCACCGTCC TCGCTGCTCT 
     1141 ATCAGCCAAA TTTCAACACC AACTTTGAAG ACAGAAATGC ATTTGTCACT GGCATTGCAA 
     1201 GATACATCGA ACAAGCAACT GTCCACTCTA GCATGAACGA GATGCTAGAG GAAGGCCAAG 
     1261 AGTATGCTGT CATGCTGTAC ACCTGGAGGA GCTGCTCCCG GGCCATCCCT CAGGTGAAGT 
     1321 GCAACGAGCA GCCGAATAGA GTTGAAATTT ATGAGAAAAC CGTGGAAGTC CTTGAACCCG 
     1381 AGGTCACAAA ACTGATGAAT TTTATGTACT TTCAGAGAAA TGCCATCGAG CGGTTCTGTG 
     1441 GGGAAGTGAG ACGCCTGTGT CATGCTGAGA GGAGAAAGGA CTTTGTTTCT GAAGCCTACT 
     1501 TGATCACCCT GGGCAAATTT ATCAACATGT TTGCTGTGTT GGATGAGCTG AAGAACATGA 
     1561 AGTGCAGTGT GAAGAATGAC CACTCTGCAT ATAAGAGGGC TGCTCAGTTT TTACGTAAAA 
     1621 TGGCAGATCC ACAATCCATC CAGGAGTCAC AGAATCTGTC CATGTTCCTG GCCAACCACA 
     1681 ACAAGATTAC ACAATCTCTG CAGCAGCAGC TTGAGGTCAT TTCTGGCTAT GAAGAGCTCC 
     1741 TGGCAGACAT CGTGAATCTG TGTGTGGATT ACTATGAGAA CAGGATGTAC TTGACACCCA 
     1801 GCGAAAAACA CATGCTTCTC AAAGTCATGG GATTTGGCCT GTACCTGATG GATGGGAGTG 
     1861 TGAGTAACAT CTACAAACTA GATGCCAAGA AAAGAATAAA TTTATCCAAA ATCGACAAGT 
     1921 ATTTCAAGCA GCTGCAGGTG GTTCCACTCT TTGGAGATAT GCAAATAGAA CTGGCAAGAT 
     1981 ACATCAAGAC CAGCGCACAC TATGAGGAGA ATAAGTCCCG GTGGACCTGC GCGTCCTCCA 
     2041 GCAGCAGCCC GCAGTACAAC ATCTGTGAGC AGATGATCCA GATCCGTGAG GACCATATGC 
     2101 GTTTCATCTC CGAGCTGGCA CGCTACAGCA ATAGCGAGGT AGTCACAGGG TCTGGCCGGC 
     2161 AGGAAGCCCA GAAGACAGAT GCTGAGTACC GGAAGCTCTT CGATCTGGCT CTGCAGGGCC 
     2221 TGCAGCTTCT GTCGCAGTGG AGCGCTCACG TGATGGAAGT GTATTCCTGG AAACTTGTAC 
     2281 ATCCAACAGA CAAGTACTCC AACAAGGACT GCCCCGACAA CGCTGAGGAG TACGAGCGAG 
     2341 CCACACGCTA CAACTACACC ACTGAGGAGA AGTTTGCCCT GGTGGAGGTG ATTGCCATGA 
     2401 TCAAAGGCCT GCAGGTGCTG ATGGGCAGGA TGGAGAGTGT GTTCAATCAC GCCATCAGAC 
     2461 ACACGGTTTA CGCTGCGCTT CAGGACTTCT CCCAGGTGAC CCTCAGGGAA CCACTGCGAC 
     2521 AGGCCATCAA GAAGAAAAAG AACGTAATCC AGAGTGTCCT ACAGGCCATC AGGAAGACTG 
     2581 TGTGTGACTG GGAGACTGGG CATGAGCCCT TTAACGACCC AGCTCTGCGG GGAGAGAAGG 
     2641 ACCCTAAGAG TGGCTTTGAC ATCAAGGTGC CACGCCGTGC TGTGGGACCC TCCAGCACGC 
     2701 AGCTTTACAT GGTGAGAACT ATGCTAGAGT CCCTCATTGC AGACAAAAGT GGTTCCAAGA 
     2761 AAACCTTGAG AAGTAGCCTT GAGGGGCCCA CCATATTGGA CATAGAAAAA TTTCATCGAG 
     2821 AATCATTCTT CTACACTCAC TTGATAAATT TCAGTGAAAC ACTGCAGCAG TGCTGTGACC 
     2881 TTTCCCAGCT GTGGTTCCGA GAGTTCTTCC TGGAGCTGAC CATGGGCAGG AGGATCCAGT 
     2941 TCCCCATTGA GATGTCTATG CCCTGGATTC TGACTGACCA CATCCTAGAG ACCAAGGAGG 
     3001 CATCAATGAT GGAGTACGTC CTCTACTCCT TGGACCTGTA CAACGACAGT GCCCACTATG 
     3061 CGCTCACCAA GTTCAACAAG CAGTTTCTCT ATGATGAGAT TGAGGCAGAG GTGAATCTGT 
     3121 GTTTTGACCA ATTTGTTTAT AAACTAGCAG ACCAGATATT TGCATATTAC AAGGTTATGG 
     3181 CAGGAAGTTT GCTTCTTGAC AAAAGGTTAC GGTCAGAATG CAAAAATCAG GGAGCCACGA 
     3241 TCCACCTCCC CCCATCTAAC CGCTATGAGA CGCTGTTGAA GCAAAGGCAC GTGCAGCTGC 
     3301 TCGGGAGATC CATTGATCTC AACCGTCTGA TTACACAGCG CGTTTCAGCA GCCATGTACA 
     3361 AATCTCTAGA ACTGGCAATT GGACGGTTTG AAAGTGAAGA TTTAACATCA GTAGTCGAGC 
     3421 TAGATGGACT CTTAGAAATC AACCGTATGA CACACAAGCT GTTGAGCAGG TACCTCACAC 
     3481 TGGACAGCTT TGATGCCATG TTCCGGGAAG CCAACCACAA CGTGTCTGCA CCTTATGGAA 
     3541 GAATTACCCT CCACGTCTTC TGGGAGCTAA ACTATGACTT CTTGCCCAAC TACTGCTATA 
     3601 ATGGCTCTAC CAACCGGTTT GTTCGGACAG TATTACCATT TTCTCAGGAA TTTCAAAGAG 
     3661 ACAAACAGCC TAACGCACAG CCCCAGTATT TGCATGGATC CAAGGCTTTG AACCTAGCCT 
     3721 ACTCAAGCAT TTATGGCAGC TACCGGAACT TTGTGGGGCC TCCGCACTTC CAAGTCATCT 
     3781 GCCGGCTACT TGGCTACCAG GGCATTGCAG TAGTCATGGA AGAGCTGCTG AAGGTTGTCA 
     3841 AGAGCCTGCT GCAAGGCACA ATCCTGCAGT ACGTGAAAAC TCTGATGGAA GTGATGCCCA 
     3901 AGATCTGCCG GCTTCCGCGA CATGAGTACG GCTCTCCTGG CATCCTGGAG TTCTTCCACC 
     3961 ACCAGCTGAA GGACATTGTG GAGTATGCAG AGCTGAAAAC CGTATGCTTC CAGAACCTGC 
     4021 GGGAGGTGGG CAATGCTGTT CTCTTCTGCC TGCTTATTGA GCAAAGCCTG TCTTTAGAAG 
     4081 AAGTCTGTGA CCTGTTGCAT GCAGCTCCTT TCCAGAATAT CTTACCTCGA ATCCATGTAA 
     4141 AAGAGGGGGA GAGAGTTGAT GCCAAAATGA AAAGACTAGA ATCCAAGTAT GCCCCACTGC 
     4201 ACCTTGTCCC ACTGATTGAA AGGCTGGGGA CCCCACAGCA AATTGCAATT GCAAGAGAGG 
     4261 GGGACTTGCT AACCAAGGAG CGTCTCTGTT GTGGTCTGTC CATGTTTGAA GTCATCCTGA 
     4321 CACGGATCCG GACCTTTCTG GATGATCCCA TCTGGCGTGG GCCCCTACCC AGCAATGGTG 
     4381 TCATGCACGT GGATGAGTGT GTGGAGTTTC ACAGACTATG GAGTGCCATG CAGTTTGTTT 
     4441 ACTGCATTCC TGTAGGGACA CACGAGTTTA CAGTGGAGCA GTGTTTTGGA GATGGGCTCC 
     4501 ACTGGGCTGG CTGCATGATC ATTGTACTTC TTGGACAGCA GCGGCGCTTT GCTGTGTTGG 
     4561 ATTTCTGCTA TCATCTTCTC AAAGTTCAGA AACATGATGG CAAAGATGAG ATCATCAAAA 
     4621 ATGTGCCATT GAAGAAGATG GTGGAGAGGA TCCGCAAGTT CCAGATTCTC AACGATGAAA 
     4681 TCATCACTAT CCTGGACAAG TACTTGAAAT CTGGTGATGG CGAGAGCACA CCTGTGGAGC 
     4741 ATGTACGCTG CTTCCAGCCA CCCATCCACC AGTCCCTAGC CAGTAGCACG CGTACGCGGC 
     4801 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4861 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4921 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4981 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5041 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5101 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5161 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5221 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5281 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5341 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5401 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5461 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5521 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5581 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5641 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5701 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5761 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5821 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5881 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5941 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6001 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6061 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6121 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6181 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6241 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6301 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6361 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6421 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6481 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6541 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6601 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6661 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6721 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6781 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6841 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6901 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6961 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7021 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7081 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7141 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7201 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7261 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7321 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7381 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7441 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7501 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7561 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7621 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7681 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7741 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7801 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7861 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7921 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7981 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8041 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8101 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8161 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8221 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8281 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8341 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8401 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8461 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8521 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8581 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//