LOCUS       MR222906                8262 bp    DNA     circular  SYN 
DEFINITION  Grip1 (Myc-DDK-tagged) - Mouse glutamate receptor interacting protein 1 (Grip1), transcript variant 1
ACCESSION   NM_028736
VERSION     NM_028736.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4419..4510 
                     /label="Myc-DDK"  
     misc_feature    4540..5125 
                     /label="PolyA signal"  
     misc_feature    4597..4616 
                     /label="Restriction Enzyme"  
     misc_feature    5401..6073 
                     /label="ColE1"  
     misc_feature    6503..7297 
                     /label="Kan/Neo"  
     misc_feature    7332..7634 
                     /label="SV40 ori"  
     misc_feature    7720..8197 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4401..4407 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4401 
                     /label="Grip1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATAGCTGTC TCTTTTAAAT GCCGCTGTCA AATTCTAAGG CGACTTACCA 
     1081 AAGATGAGAG TCCCTACACT AAATCTGCCA GCCAGACAAA GCCGCCCGAT GGAGCATTGG 
     1141 CTGTGAGGAG ACAGAGCATC CCAGAGGAAT TCAAGGGCTC CACAGTGGTG GAGTTGATGA 
     1201 AGAAGGAGGG AACCACTCTT GGCCTGACGG TATCGGGAGG CATTGATAAA GATGGCAAGC 
     1261 CAAGAGTGTC CAACCTGCGG CAGGGAGGAA TCGCTGCCAG AAGTGACCAG CTGGATGTGG 
     1321 GCGACTACAT CAAGGCGGTG AATGGGATCA ACCTGGCCAA GTTCCGCCAC GATGAGATCA 
     1381 TCAGCCTGCT GAAAAATGTC GGGGAAAGAG TGGTCCTGGA GGTCGAGTAC GAGCTTCCAC 
     1441 CGGTCTCTGT ACAAGGATCC AGTGTTATGT TCCGAACTGT GGAGGTCACG CTGCACAAAG 
     1501 AAGGCAACAC CTTTGGTTTT GTCATCCGAG GGGGAGCGCA TGATGACAGG AACAAGTCCC 
     1561 GTCCGGTTGT GATAACCTGT GTTCGTCCTG GAGGGCCTGC TGACAGAGAG GGCACCATCA 
     1621 AACCTGGAGA CAGGTTGCTC AGCGTGGATG GAATTCGGCT CCTGGGAACC ACCCATGCCG 
     1681 AGGCCATGAG CATCCTTAAA CAGTGCGGAC AAGAAGCCAC GCTGCTGATA GAATACGATG 
     1741 TCTCTGTGAT GGATTCTGTG GCGACAGCAT CCGGGCCACT ACTAGTTGAA GTTGCCAAAA 
     1801 CTCCAGGTGC CAGCCTTGGG GTTGCCCTAA CTACCTCCGT GTGCTGTAAC AAGCAGGTCA 
     1861 TTGTCATAGA CAAAATCAAA TCTGCAAGCA TTGCGGACAG ATGTGGCGCG CTACACGTGG 
     1921 GAGACCACAT CCTCTCCATT GACGGCACGA GTATGGAGTA CTGTACCCTC GCAGAAGCGA 
     1981 CCCAGTTCCT GGCCAATACC ACTGACCAGG TCAAGCTGGA GATTCTCCCA CACCATCAGA 
     2041 CCCGCCTGGC CCTAAAGGGC CCTGACCATG TGAAAATTCA GAGGAGCGAC AGACAACATC 
     2101 CCTGGGATGC CTGGGCCAGC AACCAGTGCG GCGTTCATAC CAACCATCAC CATAACACGT 
     2161 ACCACCCAGA CCATTGCAGA GTGCCAGCCC TGACTTTCCC GAAAGCGCTT CCTCCAAACA 
     2221 GCCCTCCGGC TATGGTGCCC TCATCTTCTC CTACCTCCAT GAGTGCGTAC AGTCTGAGTT 
     2281 CCCTGAACAT GGGGACTTTA CCTCGAAGCC TCTACTCCAC CAGCCCACGA GGAACCATGA 
     2341 TGAGGAGGAG ACTGAAAAAG AAAGACTTCA AAAGCTCACT GTCTTTAGCC TCCAGCACTG 
     2401 TGGGGTTGGC TGGCCAGGTC GTTCACACTG AAACCACAGA GGTTGTGCTG ACGGCTGACC 
     2461 CTGTCACGGG CTTCGGAATC CAACTGCAGG GCAGCGTGTT TGCCACAGAG ACGCTCTCCT 
     2521 CTCCGCCTCT GATTTCCTAT ATTGAAGCTG ACAGCCCAGC AGAGAGATGT GGTGTGCTAC 
     2581 AGATTGGAGA CAGAGTCATG GCCATTAATG GAATCCCAAC AGAAGACAGC ACCTTCGAGG 
     2641 AAGCCAATCA ACTCCTGAGA GACTCTTCCA TCACGAGCAA AGTCACACTA GAAATCGAGT 
     2701 TTGATGTTGC AGAGTCTGTC ATCCCAAGTA GTGGAACATT TCATGTAAAA CTGCCCAAGA 
     2761 AGCACAGCGT GGAACTTGGA ATAACCATCA GTTCGCCATC CAGTAGAAAA CCGGGGGACC 
     2821 CCCTTGTCAT TTCAGATATC AAGAAAGGCA GTGTGGCACA CAGAACCGGA ACTCTGGAAC 
     2881 TGGGAGACAA ACTGCTTGCG ATAGATAACA TCCGGCTGGA TAACTGTTCC ATGGAAGATG 
     2941 CGGTCCAGAT CCTCCAGCAG TGTGAAGACC TGGTGAAGCT CAAAATCCGC AAAGATGAAG 
     3001 ATAACTCAGA CGAGCAAGAG AGTTCCGGAG CGATTATTTA CACGGTGGAG CTGAAGCGCT 
     3061 ATGGGGGGCC CCTTGGCATC ACAATTTCTG GAACTGAAGA GCCGTTTGAT CCTATTATCA 
     3121 TCTCGAGCCT CACTAAAGGG GGATTAGCTG AAAGGACTGG AGCGATCCAC ATCGGAGATA 
     3181 GAATCCTAGC CATCAATAGC AGCAGCTTGA AGGGGAAGCC TCTGAGTGAA GCCATCCACT 
     3241 TGCTCCAGAT GGCAGGAGAG ACTGTCACCC TGAAAATTAA GAAACAGACA GATGCCCAAT 
     3301 CTGCATCAAG TCCCAAGAAG TTCCCCATCC CTGGCCACTC GGGGGACCTA GGAGATGGTG 
     3361 AGGAGGACCC CTCCCCAATA CAGAAACCTG GCAAGCTCTC CGATGCGTAC CCCTCCACGG 
     3421 TGCCCAGCGT GGACAGTGCT GTGGACTCCT GGGATGGGTC TGGAATAGAT GCCAGCTATG 
     3481 GGAGTCAAGG CTCAACTTTT CAGACTTCAG GATACAATTA CAACACCTAT GATTGGAGGA 
     3541 GTCCAAAGCA AAGAACCAGC CTGTCCCCAG TCCCCAAGCC TCGAAGCCAG ACGTACCCAG 
     3601 ATGTGGGCCT GAGTAATGAA GACTGGGATC GATCCACAGC CAGTGGCTTT GTAGGGGCTT 
     3661 CTGACAGTGC AGATGCTGAA CAAGAGGAAA ACTTCTGGTC TCAAGCATTG GAGGACCTGG 
     3721 AGACCTGCGG CCAGTCGGGG ATCCTGAGAG AGCTTGAGGA GAAAGCTGAC AGGCGTGTGT 
     3781 CATTGAGAAA CATGACCCTC TTGGCAACAA TCATGTCGGG GAGTACTATG AGTTTGAATC 
     3841 ATGAGGCTCC AATGGCTCGC AGTCAGCTGG GGCGACAGGC CAGCTTCCAG GAACGGAGCA 
     3901 GTTCACGGCC ACACTATAGC CAAACAACTC GCAGCAACAC CCTGCCCTCA GACGTGGGCA 
     3961 GAAAGTCTGT AACCCTGCGG AAAATGAAGC AAGAAATAAA GGAGATCATG TCCCCAACTC 
     4021 CGGTGGAGCT ACACAAGGTG ACCTTATACA AGGACTCTGG CATGGAGGAC TTCGGGTTCA 
     4081 GTGTGGCAGA TGGCCTGCTG GAGAAAGGCG TGTATGTCAA AAATATCCGC CCAGCTGGGC 
     4141 CAGGTGATGT TGGGGGCTTG AAGCCCTACG ACAGGCTCTT ACAGGTAAAT CACGTGCGGA 
     4201 CGAGAGACTT TGACTGCTGC CTGGTGGTGC CTCTCATAGC TGAATCTGGC AACAAGCTGG 
     4261 ACCTGGTTAT TAGCAGAAAT CCACTGGCCT CCCAGAAGTC GATAGAACAG CCGGCTCTGC 
     4321 CCAGCGACTG GAGCGAACAG AACAGCGCTT TCTTCCAGCA ACCCAGCCAC GGTGGTAATC 
     4381 TAGAGACACG AGAACCCACT AACACACTAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4441 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4501 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4561 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4621 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4681 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4741 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4801 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4861 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4921 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4981 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5041 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5101 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5161 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5221 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5281 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5341 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5401 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5461 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5521 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5581 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5641 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5701 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5761 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5821 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5881 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5941 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6001 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6061 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6121 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6181 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6241 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6301 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6361 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6421 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6481 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6541 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6601 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6661 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6721 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6781 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6841 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6901 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6961 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7021 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7081 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7141 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7201 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7261 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7321 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7381 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7441 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7501 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7561 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7621 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7681 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7741 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7801 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7861 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7921 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7981 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8041 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8101 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8161 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8221 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//