LOCUS       MR222887                8322 bp    DNA     circular  SYN 
DEFINITION  Cacna2d2 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), transcript variant 3
ACCESSION   NM_001174048
VERSION     NM_001174048.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4479..4570 
                     /label="Myc-DDK"  
     misc_feature    4600..5185 
                     /label="PolyA signal"  
     misc_feature    4657..4676 
                     /label="Restriction Enzyme"  
     misc_feature    5461..6133 
                     /label="ColE1"  
     misc_feature    6563..7357 
                     /label="Kan/Neo"  
     misc_feature    7392..7694 
                     /label="SV40 ori"  
     misc_feature    7780..8257 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4461..4467 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4461 
                     /label="Cacna2d2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGCCG GCTCGGACCT GCGGCGCTTC TTGGCCCGGC CCGGTGCGGA 
     1081 CCGCTCGCCC CTGGCCCGGT CGCGGCCCCC GGCCCTGCCC TGACCCCCGG GGCCCAGCGT 
     1141 CCGGGCCCGC ACGCCCGCTC TTGCTCTTGC TGCCGCCTCT GTTGCTTTTA CCGCTGCTCA 
     1201 CCGCCCCCGG CGCCTCTGCC TACAGCTTCC CCCAGCAGCA CACGATGCAG CACTGGGCCC 
     1261 GGCGCCTGGA GCAGGAGATT GACGGTGTGA TGCGGATTTT TGGAGGCGTG CAGCAGCTCC 
     1321 GAGAGATCTA CAAGGACAAT CGGAACCTGT TTGAAGTGCA GGAGAATGAA CCACAGAAAT 
     1381 TGGTGGAGAA AGTGGCAGGG GACATCGAGA GCCTGCTGGA CAGGAAGGTC CAGGCCCTGA 
     1441 AGAGACTGGC TGACGCTGCA GAGAATTTCC AGAAAGCCCA TCGCTGGCAG GACAACATCA 
     1501 AGGAGGAAGA CATCATGTAC TACGATGCCA AGGCTGACGC CGAGCTGGAT GACCCTGAGA 
     1561 GTGAAGATAT GGAGAGGGGA TCCAAGACCA GCGCCTTAAG GCTGGACTTC ATCGAGGACC 
     1621 CAAACTTCAA GAACAAAGTC AACTATTCAT ACACGGCTGT GCAGATCCCC ACAGACATCT 
     1681 ACAAAGGCTC TACCGTCATC CTCAATGAGC TTAACTGGAC AGAGGCCCTG GAGAACGTCT 
     1741 TCATTGAGAA CCGTAGGCAA GACCCTACAC TGTTGTGGCA AGTCTTTGGC AGTGCCACGG 
     1801 GAGTCACTCG CTATTACCCA GCCACACCAT GGCGAGCCCC CAAGAAGATT GACCTGTACG 
     1861 ATGTCAGAAG ACGACCCTGG TATATACAGG GGGCCTCATC ACCCAAGGAC ATGGTCATCA 
     1921 TTGTGGATGT GAGTGGGAGC GTGAGCGGCC TGACTCTGAA GCTGATGAAG ACGTCCGTCT 
     1981 GTGAGATGCT AGACACGCTC TCTGATGATG ACTATGTGAA CGTGGCCTCA TTCAACGAGA 
     2041 AGGCGCAGCC TGTGTCTTGC TTCACACACC TGGTGCAGGC CAATGTGCGT AACAAGAAGG 
     2101 TGTTCAAGGA AGCTGTGCAG GGCATGGTGG CCAAGGGCAC CACAGGCTAC AAGGCCGGCT 
     2161 TTGAGTATGC CTTTGACCAG CTACAGAATT CCAACATCAC GCGGGCTAAC TGCAATAAGA 
     2221 TGATCATGAT GTTCACGGAT GGGGGTGAGG ATCGCGTGCA GGATGTCTTC GAAAAGTACA 
     2281 ATTGGCCCAA TCGGACGGTA CGTGTGTTCA CGTTCTCCGT AGGACAGCAT AACTATGATG 
     2341 TCACACCCTT GCAGTGGATG GCCTGTACTA ACAAAGGTTA CTATTTTGAG ATCCCTTCCA 
     2401 TCGGAGCCAT CCGCATCAAC ACACAGGAAT ACCTGGATGT GCTGGGTAGG CCCATGGTAC 
     2461 TGGCAGGCAA GGACGCCAAG CAAGTGCAAT GGACAAACGT GTATGAAGAT GCACTGGGGC 
     2521 TGGGGTTGGT GGTAACAGGA ACTCTCCCTG TTTTCAACCT GACACAGGAT GGCCCTGGGG 
     2581 AAAAGAAGAA CCAGTTAATC CTGGGTGTCA TGGGCATCGA TGTGGCCTTG AATGACATCA 
     2641 AAAGGCTGAC TCCCAACTAC ACACTCGGCG CCAATGGCTA TGTGTTCGCC ATCGACCTGA 
     2701 ACGGCTACGT GTTGCTACAT CCCAATCTCA AGCCCCAGAC TACCAACTTC CGGGAGCCTG 
     2761 TGACCTTGGA CTTCCTGGAC GCAGAGCTGG AAGATGAGAA CAAGGAGGAG ATCCGTCGCA 
     2821 GCATGATTGA CGGCGACAAA GGCCACAAGC AGATCAGAAC CTTGGTCAAA TCCCTGGATG 
     2881 AGAGGTACAT AGACGAAGTG ATTCGGAACT ACACCTGGGT GCCTATAAGG AGTACCAACT 
     2941 ACAGCCTGGG GCTGGTGCTC CCACCCTACA GCACCTACTA CCTCCAAGCC AACCTCAGCG 
     3001 ACCAGATCCT GCAGGTCAAG TATTTTGAGT TCCTGCTTCC CAGCAGCTTT GAGTCTGAAG 
     3061 GACACGTTTT CATTGCTCCG AGAGAGTATT GCAAGGACTT GAACGCCTCG GACAACAACA 
     3121 CCGAGTTCCT GAAGAACTTC ATCGAGCTCA TGGAGAAAGT GACTCCGGAC TCCAAGCAGT 
     3181 GTAATAACTT CCTTCTTCAT AACTTGATTC TGGACACGGG CATTACACAG CAGTTAGTGG 
     3241 AACGTGTGTG GCGGGACCAA GATCTCAACA CGTACAGCCT GCTAGCCGTA TTTGCTGCCA 
     3301 CTGATGGTGG CATCACACGT GTCTTCCCGA ACAAGGCAGC CGAAGACTGG ACAGAAAACC 
     3361 CTGAACCCTT CAATGCCAGC TTCTACCGTC GCAGCCTGGA TAACCATGGT TATATCTTCA 
     3421 AGCCCCCACA CCAGGACTCC CTGTTAAGAC CACTGGAGCT GGAGAATGAC ACAGTAGGTG 
     3481 TCCTCGTCAG CACAGCTGTG GAGCTCAGTC TAGGTCGTCG CACACTGAGG CCAGCAGTGG 
     3541 TGGGTGTCAA ACTGGACCTA GAGGCTTGGG CTGAAAAGTT CAAGGTGCTG GCCAGCAACC 
     3601 GTACCCATCA GGACCAACCT CAGAAGTGCG GCCCCAGCAG CCACTGTGAG ATGGACTGCG 
     3661 AGGTTAACAA CGAGGATCTA CTCTGTGTCC TCATTGATGA CGGAGGGTTC CTGGTGCTGT 
     3721 CAAACCAGAA CCATCAGTGG GACCAGGTTG GCAGATTCTT CAGTGAGGTG GATGCCAACC 
     3781 TGATGCTGGC ACTGTACAAT AACTCCTTCT ACACCCGAAA GGAATCCTAT GACTATCAGG 
     3841 CAGCCTGTGC CCCTCAGCCT CCTGGGAACC TGGGTGCTGC ACCCCGGGGT GTCTTTGTGC 
     3901 CCACCATTGC AGATTTCCTT AACTTGGCCT GGTGGACCTC TGCTGCCGCC TGGTCCTTAT 
     3961 TCCAGCAGCT ACTCTATGGT CTCATCTATC ACAGCTGGTT CCAGGCAGAC CCGGCAGAAG 
     4021 CTGAGGGCAG CCCCGAGACG CGCGAGAGCA GCTGCGTCAT GAAACAGACC CAGTACTACT 
     4081 TCGGCTCGGT GAACGCATCC TATAATGCCA TCATTGACTG CGGAAACTGC AGCAGGCTGT 
     4141 TCCATGCGCA GAGACTGACC AACACCAATC TCCTGTTCGT GGTGGCCGAG AAGCCGCTGT 
     4201 GCAGCCAGTG CGAGGCGGGC CGGCTGCTGC AGAAGGAGAC ACACTCGGAC GGCCCGGAGC 
     4261 AGTGTGAGCT GGTGCAGAGA CCGAGATACC GAAGAGGTCC GCACATCTGT TTTGACTACA 
     4321 ATGCGACGGA AGATACCTCA GACTGTGGCC GCGGAGCCTC CTTCCCTCCG TCGCTGGGCG 
     4381 TCTTGGTTTC CTTGCAGCTT TTGCTCCTCC TGGGCCTGCC ACCTCGGCCG CAGCCTCAAG 
     4441 TCCACTCCTT CGCTGCCTCT CGCCACCTCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4501 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4561 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4621 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4681 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4741 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4801 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4861 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4921 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4981 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5041 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5101 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5161 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5221 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5281 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5341 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5401 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5461 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5521 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5581 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5641 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5701 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5761 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5821 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5881 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5941 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6001 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6061 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6121 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6181 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6241 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6301 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6361 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6421 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6481 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6541 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6601 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6661 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6721 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6781 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6841 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6901 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6961 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7021 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7081 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7141 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7201 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7261 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7321 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7381 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7441 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7501 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7561 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7741 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7801 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7861 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7921 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7981 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8041 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8101 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8161 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8221 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8281 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//