LOCUS       MR222863                8691 bp    DNA     circular  SYN 
DEFINITION  Bcr (Myc-DDK-tagged) - Mouse breakpoint cluster region (Bcr)
ACCESSION   NM_001081412
VERSION     NM_001081412.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4848..4939 
                     /label="Myc-DDK"  
     misc_feature    4969..5554 
                     /label="PolyA signal"  
     misc_feature    5026..5045 
                     /label="Restriction Enzyme"  
     misc_feature    5830..6502 
                     /label="ColE1"  
     misc_feature    6932..7726 
                     /label="Kan/Neo"  
     misc_feature    7761..8063 
                     /label="SV40 ori"  
     misc_feature    8149..8626 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4830..4836 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4830 
                     /label="Bcr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGACTCG GTGGGCTTCG CCGAGGCGTG GAGGGCGCAG TTCCCGGACT 
     1081 CGGAGCCGCC GCGCATGGAG CTGCGCTCGG TGGGTGACAT CGAGCAGGAG CTGGAGCGCT 
     1141 GCAAGGCCTC CATTAGGCGC TTGGAGCAGG AGGTGAACCA GGAGCGCTTC CGCATGATCT 
     1201 ACCTGCAGAC GCTGCTGGCC AAGGAGAAGA AGAGCTACGA TCGGCAGCGC TGGGGCTTCC 
     1261 GCCGTGCAGC GCAGCCCCCC GACGGCGCCG CCGAGCCCCG AGCGTCCGCC CCGCGCCCGC 
     1321 CGCCCGCGCC CGCCGACGGC GCAGACCCGG CGCCCGTCGA GGAGTCCGAG GCGCGGCCGG 
     1381 ACGGAGAGGG GTCACCGAGT AAGGGGCGGT CAGCGTCCGC CCGCAGGCCA GCGGCTGCTG 
     1441 CATCGGCGGA CCGGGACGAC CGAGGGCCCC CCACCAGCGT GGCGGCGCTC AGATCCAACT 
     1501 TCGAGAAGAT CCGCAAGGGA CCTGCCCAGC CCGGCTCTGC CGACGCCGAG AAGCCCTTCT 
     1561 ACGTGAACGT CGAGTTCCAC CACGAGCGCG GCCTGGTGAA GGTCAACGAC AAAGAGGTGT 
     1621 CCGACCGAAT CAGTTCCCTG GGCAGCCAGG CCATGCAAAT GGAGCGCAAG AAGTCCCAGC 
     1681 AGAGCGCCGG GCAGGGCCTG GGGGAAGCAC CCAGACCCCA TTACCGTGGG CGCTCCTCGG 
     1741 AAAGCAGTTG CGGCCTTGAC GGTGACTATG AGGATGCAGA GTTGAATCCC CGCTTCCTGA 
     1801 AGGACAACCT AATAAATGCC AACGGCGGCA ACAGGCCCCC TTGGCCGCCC CTGGAGTACC 
     1861 AACCCTACCA GAGCATCTAT GTCGGAGGCA TGATGGTGGA AGGGGAGGGC AAGAGCCCTC 
     1921 TCCTGCGAAG CCAGAGCACT TCAGAGCAGG AGAAACGCCT AACCTGGCCT CGCAGGTCCT 
     1981 ACTCGCCTCG GAGTTTCGAG GACAGCGGAG GTGGCTACAC GCCGGACTGC AGCTCCAACG 
     2041 AGAACCTCAC CTCCAGCGAG GAGGACTTTT CCTCTGGCCA GTCCAGCCGC GTGTCACCAA 
     2101 GCCCCACCAC CTACCGCATG TTCCGGGACA AGAGCCGCTC ACCCTCGCAG AATTCGCAGC 
     2161 AGTCCTTTGA CAGCAGCAGC CCCCCGACGC CACAGTGCCA GAAGCGGCAC CGGCAGTGCC 
     2221 AGGTTGTGGT GTCTGAGGCT ACCATCGTGG GTGTCCGCAA GACTGGGCAG ATCTGGCCCA 
     2281 GTGATGGGGA CAGCACCTTT CAAGGGGAAG CAGATTCCTC GTTTGGAACA CCACCTGGAT 
     2341 ATGGCTGTGC CGCAGACCAG GCTGAGGAAC AGCGCCGGCA CCAGGACGGG CTGCCCTACA 
     2401 TCGATGATTC ACCTTCTTCC TCGCCACACC TCAGCAGCAA AGGCAGAGGC AGCCTGGCCT 
     2461 CAGGCGCCCT GGATCCCACC AAAGTGAGTG AGCTGGATCT GGAAAAGGGC CTGGAGATGA 
     2521 GAAAGTGGGT TTTGTCCGGA ATCCTGGCCA GCGAGGAGAC GTACCTGAGC CATCTGGAGG 
     2581 CCCTGCTGCT GCCCATGAAG CCTCTGAAAG CCGCTGCCAC CACCTCTCAG CCTGTGCTGA 
     2641 CCAGCCAGCA GATTGAGACC ATTTTCTTCA AAGTGCCTGA GCTCTACGAG ATCCACAAGG 
     2701 AGTTCTACGA TGGGCTGTTC CCCCGAGTGC AGCAGTGGAG CCACCAGCAG CGTGTAGGGG 
     2761 ACCTCTTCCA GAAGCTGGCC AGCCAGCTGG GTGTGTACCG AGCCTTTGTG GATAACTACG 
     2821 GAGTGGCCAT GGAAACTGCA GAGAAGTGCT GTCAGGCCAA CGCTCAGTTT GCAGAAATCT 
     2881 CTGAGAACCT GCGAGCCAGA AGCAACAAGG ATGTCAAGGA CTCAACTACC AAGAACTCTT 
     2941 TGGAAACTCT GCTCTACAAA CCTGTGGACC GGGTGACGAG GAGCACGCTA GTCCTTCACG 
     3001 ACTTGCTAAA ACACACCCCG TCCAGCCATC CCGACCATTC CCTGCTGCAA GACGCCCTCC 
     3061 GAATCTCTCA GAACTTCCTG TCCAGCATCA ACGAGGAGAT CACGCCCCGC CGACAGTCCA 
     3121 TGACGGTGAA AAAAGGAGAG CACCGGCAGC TGCTGAAGGA CAGCTTTATG GTAGAGCTGG 
     3181 TGGAGGGGGC CCGCAAGCTG CGTCACATCT TCCTGTTCAC TGACCTGCTC CTCTGCACCA 
     3241 AGCTGAAGAA GCAAAGTGGA GGCAAAACCC AGCAATATGA CTGTAAATGG TACATTCCGC 
     3301 TCACGGACCT CAGCTTCCAG ATGGTGGATG AATTGGAGGC ACTACCCAAC ATCCCTCTGG 
     3361 TGCCAGATGA GGAACTGGAT GCCTTGAAGA TCAAAATCTC CCAAATCAAG AGCGACATCC 
     3421 AACGAGAGAA GAGAGCAAAT AAGGGCAGCA AGGTCATGGA GAGGCTGAGG AAGAAGCTGT 
     3481 CAGAGCAGGA GTCACTGCTG CTGTTAATGT CTCCCAGCAT GGCCTTCCGC GTTCACAGTC 
     3541 GCAACGGCAA GAGTTACACG TTCCTTATTT CCTCTGACTA TGAGCGAGCC GAGTGGAGAG 
     3601 AAAGCATCCG AGAGCAGCAG AAGAAATGTT TCAAAAGTTT CTCTCTGACA TCTGTGGAGC 
     3661 TACAAATGCT GACCAACTCA TGTGTAAAAC TTCAGACGGT TCACCACATT CCACTGACCA 
     3721 TCAACAAAGA AGATGACGAG TCTCCTGGAC TCTACGGCTT CCTGCATGTC ATCGTCCACT 
     3781 CAGCCACCGG CTTTAAGCAG AGCTCAAATC TCTACTGCAC CCTGGAGGTG GATTCCTTTG 
     3841 GATATTTTGT GAATAAAGCA AAGACTCGAG TCTACAGGGA CACAACTGAG CCAAACTGGA 
     3901 ATGAGGAGTT TGAGATAGAG CTAGAAGGCT CCCAGACCCT GCGGATACTA TGTTATGAAA 
     3961 AGTGTTACAA CAAAATGAAG ATGACGAAGG AAGATGGTGA GAGCGCAGAC AAGCTCATGG 
     4021 GGAAAGGCCA GGTCCAGCTG GACCCCCAAA CCCTACAGGA CAGAGACTGG CAGCGGACTG 
     4081 TCATCGACAT GAATGGAATT GAAGTGAAAC TCTCCGTCAA GTTCACGAGC AGGGAGTTCA 
     4141 GCCTGAAGAG AATGCCCTCC CGCAAACAGA CAGGGGTCTT CGGTGTCAAG ATTGCTGTAG 
     4201 TCACAAAGAG AGAGAGGTCC AAGGTACCTT ACATTGTGCG CCAGTGTGTG GAAGAGATCG 
     4261 AGCGCCGGGG TATGGAGGAG GTGGGCATCT ACCGAGTGTC TGGAGTGGCC ACAGACATCC 
     4321 AGGCACTGAA GGCAGCCTTT GATGTCAATA ACAAGGATGT GTCCGTGATG ATGAGCGAGA 
     4381 TGGACGTGAA CGCCATTGCC GGCACGCTGA AGCTCTACTT CCGTGAGCTG CCCGAGCCCC 
     4441 TCTTCACGGA TGAGTTCTAC CCTAACTTCG CAGAAGGCAT TGCTCTTTCA GACCCCGTCG 
     4501 CAAAGGAGAG CTGCATGCTG AACCTGCTGC TGTCCCTCCC AGAAGCCAAC CTGCTCACCT 
     4561 TCCTCTTCCT CCTGGACCAC CTGAAAAGGG TAGCTGAAAA GGAGACGGTG AACAAGATGT 
     4621 CCCTGCACAA CCTCGCCACG GTCTTTGGCC CCACACTGCT TCGGCCCTCA GAGAAGGAAA 
     4681 GCAAGCTCCC TGCTAACCCC AGCCAGCCCA TCACCATGAC TGACAGCTGG TCCCTGGAGG 
     4741 TCATGTCCCA GGTCCAAGTG TTGCTGTACT TCCTGCAGCT GGAGGCCATC CCTGCCCCTG 
     4801 ACAGCAAGAG ACAGAGCATC CTGTTTTCCA CTGAAGTCAC GCGTACGCGG CCGCTCGAGC 
     4861 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4921 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4981 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5041 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5101 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5161 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5221 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5281 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5341 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5401 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5461 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5521 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5581 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5641 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5701 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5761 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5821 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5881 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5941 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6001 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6061 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6121 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6181 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6241 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6301 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6361 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6421 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6481 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6541 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6601 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6661 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6721 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6781 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6841 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6901 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6961 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7021 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7081 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7141 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7201 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7261 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7321 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7381 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7441 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7501 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7561 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7621 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7681 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7741 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7801 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7861 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7921 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7981 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8041 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8101 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8161 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8221 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8281 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8341 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8401 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8461 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8521 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8581 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8641 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//