LOCUS       MR222801                8343 bp    DNA     circular  SYN 
DEFINITION  Cacna2d2 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), transcript variant 2
ACCESSION   NM_020263
VERSION     NM_020263.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4500..4591 
                     /label="Myc-DDK"  
     misc_feature    4621..5206 
                     /label="PolyA signal"  
     misc_feature    4678..4697 
                     /label="Restriction Enzyme"  
     misc_feature    5482..6154 
                     /label="ColE1"  
     misc_feature    6584..7378 
                     /label="Kan/Neo"  
     misc_feature    7413..7715 
                     /label="SV40 ori"  
     misc_feature    7801..8278 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4482..4488 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4482 
                     /label="Cacna2d2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGTGCCG GCTCGGACCT GCGGCGCTTC TTGGCCCGGC CCGGTGCGGA 
     1081 CCGCTCGCCC CTGGCCCGGT CGCGGCCCCC GGCCCTGCCC TGACCCCCGG GGCCCAGCGT 
     1141 CCGGGCCCGC ACGCCCGCTC TTGCTCTTGC TGCCGCCTCT GTTGCTTTTA CCTCTGCTCA 
     1201 CCGCCCCCGG CGCCTCTGCC TACAGCTTCC CCCAGCAGCA CACGATGCAG CACTGGGCCC 
     1261 GGCGCCTGGA GCAGGAGATT GACGGTGTGA TGCGGATTTT TGGAGGCGTG CAGCAGCTCC 
     1321 GAGAGATCTA CAAGGACAAT CGGAACCTGT TTGAAGTGCA GGAGAATGAA CCACAGAAAT 
     1381 TGGTGGAGAA AGTGGCAGGG GACATCGAGA GCCTGCTGGA CAGGAAGGTC CAGGCCCTGA 
     1441 AGAGACTGGC TGACGCTGCA GAGAATTTCC AGAAAGCCCA TCGCTGGCAG GACAACATCA 
     1501 AGGAGGAAGA CATCATGTAC TACGATGCCA AGGCTGACGC CGAGCTGGAT GACCCTGAGA 
     1561 GTGAAGATAT GGAGAGGGGA TCCAAGACCA GCGCCTTAAG GCTGGACTTC ATCGAGGACC 
     1621 CAAACTTCAA GAACAAAGTC AACTATTCAT ACACGGCTGT GCAGATCCCC ACAGACATCT 
     1681 ACAAAGGCTC TACCGTCATC CTCAATGAGC TTAACTGGAC AGAGGCCCTG GAGAACGTCT 
     1741 TCATTGAGAA CCGTAGGCAA GACCCTACAC TGTTGTGGCA AGTCTTTGGC AGTGCCACGG 
     1801 GAGTCACTCG CTATTACCCA GCCACACCAT GGCGAGCCCC CAAGAAGATT GACCTGTACG 
     1861 ATGTCAGAAG ACGACCCTGG TATATACAGG GGGCCTCATC ACCCAAGGAC ATGGTCATCA 
     1921 TTGTGGATGT GAGTGGCAGC GTGAGCGGCC TGACTCTGAA GCTGATGAAG ACGTCCGTCT 
     1981 GTGAGATGCT AGACACGCTC TCTGATGATG ACTATGTGAA CGTGGCCTCA TTCAACGAGA 
     2041 AGGCGCAGCC TGTGTCTTGC TTCACACACC TGGTGCAGGC CAATGTGCGT AACAAGAAGG 
     2101 TGTTCAAGGA AGCTGTGCAG GGCATGGTGG CCAAGGGCAC CACAGGCTAC AAGGCCGGCT 
     2161 TTGAGTATGC CTTTGACCAG CTACAGAATT CCAACATCAC GCGGGCTAAC TGCAATAAGA 
     2221 TGATCATGAT GTTCACGGAT GGGGGTGAGG ATCGCGTGCA GGATGTCTTC GAAAAGTACA 
     2281 ATTGGCCCAA TCGGACGGTA CGTGTGTTCA CGTTCTCCGT AGGACAGCAT AACTATGATG 
     2341 TCACACCCTT GCAGTGGATG GCCTGTACTA ACAAAGGTTA CTATTTTGAG ATCCCTTCCA 
     2401 TCGGAGCCAT CCGCATCAAC ACACAGGAAT ACCTGGATGT GCTGGGTAGG CCCATGGTAC 
     2461 TGGCAGGCAA GGACGCCAAG CAAGTGCAAT GGACAAACGT GTATGAAGAT GCACTGGGGC 
     2521 TGGGGTTGGT GGTAACAGGA ACTCTCCCTG TTTTCAACCT GACACAGGAT GGCCCTGGGG 
     2581 AAAAGAAGAA CCAGTTAATC CTGGGTGTCA TGGGCATCGA TGTGGCCTTG AATGACATCA 
     2641 AAAGGCTGAC TCCCAACTAC ACACTCGGCG CCAATGGCTA TGTGTTCGCC ATCGACCTGA 
     2701 ACGGCTACGT GTTGCTACAT CCCAATCTCA AGCCCCAGAC TACCAACTTC CGGGAGCCTG 
     2761 TGACCTTGGA CTTCCTGGAC GCAGAGCTGG AAGATGAGAA CAAGGAGGAG ATCCGTCGCA 
     2821 GCATGATTGA CGGCGACAAA GGCCACAAGC AGATCAGAAC CTTGGTCAAA TCCCTGGATG 
     2881 AGAGGTACAT AGACGAAGTG ATTCGGAACT ACACCTGGGT GCCTATAAGG AGTACCAACT 
     2941 ACAGCCTGGG GCTGGTGCTC CCACCCTACA GCACCTACTA CCTCCAAGCC AACCTCAGCG 
     3001 ACCAGATCCT GCAGGTCAAG TTGCCAATCA GCAAACTGAA GGATTTTGAG TTCCTGCTTC 
     3061 CCAGCAGCTT TGAGTCTGAA GGACACGTTT TCATTGCTCC GAGAGAGTAT TGCAAGGACT 
     3121 TGAACGCCTC GGACAACAAC ACCGAGTTCC TGAAGAACTT CATCGAGCTC ATGGAGAAAG 
     3181 TGACTCCGGA CTCCAAGCAG TGTAATAACT TCCTTCTTCA TAACTTGATT CTGGACACGG 
     3241 GCATTACACA GCAGTTAGTG GAACGTGTGT GGCGGGACCA AGATCTCAAC ACGTACAGCC 
     3301 TGCTAGCCGT ATTTGCTGCC ACTGATGGTG GCATCACACG TGTCTTCCCG AACAAGGCAG 
     3361 CCGAAGACTG GACAGAAAAC CCTGAACCCT TCAATGCCAG CTTCTACCGT CGCAGCCTGG 
     3421 ATAACCATGG TTATATCTTC AAGCCCCCAC ACCAGGACTC CCTGTTAAGA CCACTGGAGC 
     3481 TGGAGAATGA CACAGTAGGT GTCCTCGTCA GCACAGCTGT GGAGCTCAGT CTAGGTCGTC 
     3541 GCACACTGAG GCCAGCAGTG GTGGGTGTCA AACTGGACCT AGAGGCTTGG GCTGAAAAGT 
     3601 TCAAGGTGCT GGCCAGCAAC CGTACCCATC AGGACCAACC TCAGAAGTGC GGCCCCAGCA 
     3661 GCCACTGTGA GATGGACTGC GAGGTTAACA ACGAGGATCT ACTCTGTGTC CTCATTGATG 
     3721 ACGGAGGGTT CCTGGTGCTG TCAAACCAGA ACCATCAGTG GGACCAGGTT GGCAGATTCT 
     3781 TCAGTGAGGT GGATGCCAAC CTGATGCTGG CACTGTACAA TAACTCCTTC TACACCCGAA 
     3841 AGGAATCCTA TGACTATCAG GCAGCCTGTG CCCCTCAGCC TCCTGGGAAC CTGGGTGCTG 
     3901 CACCCCGGGG TGTCTTTGTG CCCACCATTG CAGATTTCCT TAACTTGGCC TGGTGGACCT 
     3961 CTGCTGCCGC CTGGTCCTTA TTCCAGCAGC TACTCTATGG TCTCATCTAT CACAGCTGGT 
     4021 TCCAGGCAGA CCCGGCAGAA GCTGAGGGCA GCCCCGAGAC GCGCGAGAGC AGCTGCGTCA 
     4081 TGAAACAGAC CCAGTACTAC TTCGGCTCGG TGAACGCATC CTATAATGCC ATCATTGACT 
     4141 GCGGAAACTG CAGCAGGCTG TTCCATGCGC AGAGACTGAC CAACACCAAT CTCCTGTTCG 
     4201 TGGTGGCCGA GAAGCCGCTG TGCAGCCAGT GCGAGGCGGG CCGGCTGCTG CAGAAGGAGA 
     4261 CACACTCGGA CGGCCCGGAG CAGTGTGAGC TGGTGCAGAG ACCGAGATAC CGAAGAGGTC 
     4321 CGCACATCTG TTTTGACTAC AATGCGACGG AAGATACCTC AGACTGTGGC CGCGGAGCCT 
     4381 CCTTCCCTCC GTCGCTGGGC GTCTTGGTTT CCTTGCAGCT TTTGCTCCTC CTGGGCCTGC 
     4441 CACCTCGGCC GCAGCCTCAA GTCCACTCCT TCGCTGCCTC TCGCCACCTC ACGCGTACGC 
     4501 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4561 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4621 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4681 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4741 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4801 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4861 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4921 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4981 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5041 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5101 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5161 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5221 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5281 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5341 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5401 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5461 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5521 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5581 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5641 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5701 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5761 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5821 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5881 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5941 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6001 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6061 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6121 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6181 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6241 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6301 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6361 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6421 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6481 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6541 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6601 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6661 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6721 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6781 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6841 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6901 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6961 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7021 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7081 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7141 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7201 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7261 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7321 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7381 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7441 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7501 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7561 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7741 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7801 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7861 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7921 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7981 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8041 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8101 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8161 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8221 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8281 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8341 TTT
//