LOCUS       MR222727                8799 bp    DNA     circular  SYN 
DEFINITION  Mapk8ip3 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3), transcript variant 5
ACCESSION   NM_001163450
VERSION     NM_001163450.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4956..5047 
                     /label="Myc-DDK"  
     misc_feature    5077..5662 
                     /label="PolyA signal"  
     misc_feature    5134..5153 
                     /label="Restriction Enzyme"  
     misc_feature    5938..6610 
                     /label="ColE1"  
     misc_feature    7040..7834 
                     /label="Kan/Neo"  
     misc_feature    7869..8171 
                     /label="SV40 ori"  
     misc_feature    8257..8734 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4938..4944 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4938 
                     /label="Mapk8ip3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGGAGATC CAGATGGACG AGGGAGGAGG AGTGGTGGTG TACCAAGACG 
     1081 ACTACTGCTC GGGCTCGGTC ATGTCGGAGC GTGTGTCGGG CCTGGCGGGC TCCATCTACC 
     1141 GCGAGTTCGA GCGCCTCATT CACTGCTATG ACGAGGAGGT GGTCAAGGAG CTCATGCCGC 
     1201 TGGTGGTGAA CGTGCTGGAG AACCTTGACT CGGTGCTGAG CGAGAACCAG GAGCACGAGG 
     1261 TGGAGCTGGA GCTCCTACGC GAGGACAACG AGCAGCTGCT CACGCAATAC GAGCGCGAGA 
     1321 AGGCGCTGCG CAAACAGGCC GAGGAGAAAT TCATCGAATT TGAAGATGCC TTGGAACAAG 
     1381 AGAAGAAAGA ACTCCAGATC CAGGTAGAAC ATTATGAGTT TCAGACACGC CAGCTGGAGC 
     1441 TAAAGGCCAA AAACTATGCA GATCAGATTT CCCGACTGGA GGAACGAGAA TCGGAGATGA 
     1501 AGAAGGAATA CAATGCCCTG CACCAGCGGC ACACAGAGAT GATCCAGACC TATGTGGAAC 
     1561 ACATTGAAAG ATCCAAGATG CAGCAAGTTG GGGGTAGCGG CCAAACAGAA AGCAGCCTGC 
     1621 CCGGGCGGAG CAGGAAGGAG CGTCCCACCT CTCTGAATGT CTTCCCCCTG GCTGATGGCA 
     1681 TGTGTCCAAA TGATGAGATG TCTGAGTCAG GCCAGTCCTC AGCAGCTGCA ACACCCAGTA 
     1741 CCACAGGTAC CAAGTCCAAC ACACCCACGT CCTCCGTGCC CTCAGCAGCA GTCACGCCAC 
     1801 TCAACGAGAG CCTACAGCCC CTGGGGGACT ATGTCAGTGT CACAAAGAAC AACAAGCAGG 
     1861 CCCGAGAGAA GCGCAATAGC CGTAACATGG AGGTCCAGGT CACCCAAGAG ATGCGGAACG 
     1921 TCAGTATCGG CATGGGCAGC AGTGACGAGT GGTCCGATGT TCAGGACATT ATCGACTCCA 
     1981 CCCCAGAGCT GGATGTGTGT CCTGAAACCC GTCTGGAGCG CACAGGAAGC AGCCCAACCC 
     2041 AGGGAATTGT AAACAAAGCT TTTGGAATCA ACACTGACTC CTTGTATCAC GAACTCTCCA 
     2101 CGGCGGGATC TGAGGTCATC GGGGATGTGG ACGAGGGAGC TGATCTCCTA GGGGAGTTTT 
     2161 CAGTGCGCGA TGATTTTTTT GGAATGGGCA AAGAAGTGGG GAACCTGCTG CTGGAGAACT 
     2221 CACAGCTTCT AGAGACAAAA AATGCTTTAA ATGTAGTGAA GAATGACCTC ATTGCTAAGG 
     2281 TTGACCAACT GTCAGGAGAA CAGGAGGTCC TGAAGGGTGA GCTGGAAGCA GCCAAGCAAG 
     2341 CCAAAGTCAA GCTGGAGAAC CGAATCAAAG AGCTTGAAGA AGAACTGAAG AGAGTCAAGT 
     2401 CAGAGGCAGT AACTGCCCGC CGTGAGCCCA GAGAAGAGGT GGAGGATGTA AGCAGCTATC 
     2461 TCTGTACAGA ATTGGACAAA ATCCCCATGG CCCAGCGCCG ACGCTTCACA CGGGTGGAGA 
     2521 TGGCCCGAGT GCTCATGGAA CGCAACCAGT ACAAGGAACG CCTCATGGAG CTGCAGGAGG 
     2581 CTGTGAGGTG GACTGAAATG ATCAGAGCAT CAAGGGAACA CCCATCTGTC CAGGAGAAGA 
     2641 AGAAGTCCAC CATCTGGCAG TTCTTTAGTC GCCTCTTCAG CTCCTCATCT AGCCCCCCTC 
     2701 CGGCCAAACG ATCCTACCCA TCTGTGAACA TTCACTACAA GTCACCCACT GCAGCTGGCT 
     2761 TTAGCCAGCG TCGCAGCCAT GCTTTGTGCC AGATCTCAGC CGGCAGCAGG CCCCTGGAGT 
     2821 TCTTCCCTGA TGATGACTGC ACCTCTTCTG CCCGGCGGGA GCAGAAGCGG GAGCAGTACC 
     2881 GCCAGGTTCG TGAACACGTG CGCAATGATG ACGGGAGGCT GCAGGCCTGT GGGTGGAGCC 
     2941 TGCCTGCCAA GTACAAGCAG CTGAGCCCCA ATGGAGGCCA GGAAGACACC CGGATGAAAA 
     3001 ATGTGCCTGT CCCTGTGTAC TGCCGCCCTC TGGTGGAGAA GGACCCTTCG ACAAAGCTGT 
     3061 GGTGTGCTGC TGGTGTCAAC CTGAGTGGGT GGAAGCCACA TGAAGAGGAC TCTAGCAATG 
     3121 GACCCAAGCC TGTACCAGGT CGAGACCCTC TGACCTGTGA CCGGGAAGGA GAAGGCGAAC 
     3181 CCAAGAGCAC ACACCCATCA CCTGAGAAGA AGAAGGCAAA GGAAACCCCT GAGGCAGATG 
     3241 CTACCTCCAG TCGGGTATGG ATCCTCACCA GCACCCTGAC AACCAGCAAG GTGGTGATCA 
     3301 TTGATGCCAA CCAGCCAGGC ACAATTGTGG ATCAGTTCAC AGTCTGCAAT GCCCACGTCC 
     3361 TGTGTATCTC CAGCATTCCT GCGGCCAGTG ACAGTGACTA TCCCCCTGGG GAGATGTTCC 
     3421 TAGACAGTGA TGTGAACCCT GAAGATTCAG GTGCTGATGG TGTGCTGGCT GGCATCACCC 
     3481 TGGTGGGGTG TGCTACCCGC TGCAATGTTC CACGTAGCAA CTGTTCCTCA CGAGGAGACA 
     3541 CCCCAGTACT GGACAAGGGG CAGGGGGATG TGGCGACCAC TGCCAATGGG AAGGTCAACC 
     3601 CGTCCCAATC CACAGAAGAA GCCACAGAAG CCACGGAGGT GCCAGACCCT GGTCCCAGCG 
     3661 AGTCAGAAGC AACGACAGTC CGGCCCGGGC CTCTCACAGA GCATGTCTTT ACTGACCCAG 
     3721 CACCCACCCC ATCCTCCAGC ACCCAGCCTG CCAGTGAGAA TGGGTCAGAG TCCAATGGCA 
     3781 CCATTGTACA GCCTCAGGTG GAGCCCAGTG GGGAACTCTC AACAACAACC AGTAGCGCTG 
     3841 CACCCACTAT GTGGCTAGGA GCCCAGAATG GCTGGCTCTA TGTGCATTCA GCGGTAGCCA 
     3901 ACTGGAAGAA GTGTCTGCAC TCCATCAAGC TAAAAGACTC TGTGCTGAGC CTGGTGCATG 
     3961 TCAAAGGCCG AGTGCTGGTA GCTCTTGCAG ATGGGACCCT GGCTATCTTC CATCGTGGAG 
     4021 AGGATGGCCA GTGGGACCTG AGCAACTACC ACCTAATGGA CCTGGGCCAC CCACACCACT 
     4081 CCATCCGCTG CATGGCTGTT GTGAATGACC GAGTTTGGTG TGGCTACAAG AACAAGGTGC 
     4141 ATGTTATCCA GCCCAAGACA ATGCAGATTG AGAAATCATT TGATGCCCAC CCAAGGCGGG 
     4201 AAAGCCAGGT ACGTCAGCTG GCCTGGATCG GTGATGGAGT GTGGGTCTCT ATTCGCTTGG 
     4261 ATTCTACCCT TCGGCTCTAC CATGCTCACA CCCACCAGCA CCTGCAGGAT GTGGACATTG 
     4321 AGCCCTATGT TAGCAAGATG CTAGGAACCG GCAAGCTGGG CTTCTCCTTC GTGCGCATCA 
     4381 CAGCCTTACT CATTGCAGGC AACCGTCTGT GGGTGGGCAC TGGCAATGGG GTTGTCATCT 
     4441 CCATCCCCTT GACTGAGACT GTGGTCCTGC ATCGAGGCCA GCTCCTAGGG CTCCGAGCCA 
     4501 ACAAGACATC CCCAACATCT GGGGAGGGGA CCCGCCCAGG GGGCATCATC CATGTGTATG 
     4561 GGGACGACAG CAGTGACAAG GCCGCCAGTA GTTTCATCCC CTACTGCTCC ATGGCACAGG 
     4621 CTCAGCTTTG CTTCCATGGG CACCGTGATG CTGTCAAATT CTTTGTCTCT GTGCCAGGAA 
     4681 ATGTGCTGGC CACTCTCAAT GGCAGTGTGC TAGACAGCCC ATCAGAGGGC CCTGGGCCTG 
     4741 CTGCACCCGC TGCAGATGCT GAGGGCCAGA AGTTGAAGAA TGCACTGGTG CTGAGTGGTG 
     4801 GTGAAGGTTA CATTGACTTC CGTATCGGAG ACGGAGAGGA TGATGAAACT GAGGAATGTG 
     4861 CCGGGGACGT GAACCAGACA AAGCCCTCGT TGTCCAAGGC TGAGCGCAGC CACATCATCG 
     4921 TGTGGCAGGT GTCCTACACC CCTGAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4981 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     5041 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     5101 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     5161 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     5221 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5281 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5341 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5401 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5461 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5521 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5581 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5641 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5701 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5761 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5821 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5881 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5941 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     6001 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     6061 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     6121 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     6181 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6241 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6301 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6361 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6421 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6481 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6541 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6601 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6661 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6721 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6781 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6841 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6901 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6961 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     7021 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     7081 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     7141 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     7201 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7261 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7321 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7381 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7441 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7501 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7561 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7621 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7681 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7741 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7801 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7861 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7921 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7981 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     8041 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8101 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8161 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     8221 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8281 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8341 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8401 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8461 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8521 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8581 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8641 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8701 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8761 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//