LOCUS       MR222717                8469 bp    DNA     circular  SYN 
DEFINITION  Lamc2 (Myc-DDK-tagged) - Mouse laminin, gamma 2 (Lamc2)
ACCESSION   NM_008485
VERSION     NM_008485.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4624..4715 
                     /label="Myc-DDK"  
     misc_feature    4745..5330 
                     /label="PolyA signal"  
     misc_feature    4802..4821 
                     /label="Restriction Enzyme"  
     misc_feature    5606..6278 
                     /label="ColE1"  
     misc_feature    6708..7502 
                     /label="Kan/Neo"  
     misc_feature    7537..7839 
                     /label="SV40 ori"  
     misc_feature    7925..8402 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4599..4606 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4599 
                     /label="Lamc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCGCTC TGGCTCAGCT GCTGCCTTGG TGTCGCGCTC CTGCTGCCCG 
     1081 CAGCCCAGGC CACCTCCAGG AGGGAAGTCT GTGATTGCAA TGGGAAGTCC AGGCAATGTG 
     1141 TCTTTGATCA GGAGCTCCAT CGACAGACAG GCAGCGGGTT CCGTTGCCTC AACTGCAATG 
     1201 ACAATACAGC GGGGGTTCAC TGCGAGCGGT GCAGGGAGGG GTTTTACCGA CATCGAGACA 
     1261 GAGACCGCTG CCTACCCTGC AACTGCCACT CAAAGGGTTC CCTCAGTGCT GGATGTGACA 
     1321 ACTCTGGACA ATGCAGGTGT AAGCCAGGTG TGACAGGACA AAGATGTGAC CGATGTCAGC 
     1381 CAGGCTTCCA TATGCTCACC GATGCTGGAT GCACCCGAGA CCAGGGGCAA CTAGATTCCA 
     1441 AGTGTGACTG TGACCCAGCT GGCATCTCTG GACCCTGTGA TTCTGGCCGA TGTGTCTGCA 
     1501 AACCAGCCGT CACTGGAGAG CGCTGTGATA GGTGCCGACC AGGCTACTAT CATCTGGACC 
     1561 GGGCAAACCC TGAGGGCTGT ACCCAGTGTT TCTGCTATGG GCATTCAGCC AGCTGCCACG 
     1621 CCTCTGCCGA CTTCAGTGTC CACAAAATCA CTTCAACTTT CAGTCAGGAT GTGGATGGTT 
     1681 GGAAGGCGGT TCAGAGAAAC GGGGCACCTG CAAAACTCCA CTGGTCACAG CGCCATCGGG 
     1741 ACGTGTTTAG TTCTGCCCGA AGATCAGACC CCGTCTATTT CGTGGCCCCT GCCAAATTCC 
     1801 TCGGTAACCA GCAAGTGAGT TACGGGCAGA GCCTGTCTTT TGACTACCGC GTGGACAGAG 
     1861 GAGGTAGACA GCCGTCTGCC TACGATGTGA TCCTGGAAGG TGCTGGTCTA CAGATCAGAG 
     1921 CTCCTCTGAT GGCTCCAGGC AAGACACTTC CCTGTGGGAT CACAAAGACT TACACATTCA 
     1981 GACTGAATGA ACATCCAAGC AGTCACTGGA GTCCCCAGCT GAGTTATTTC GAATATCGAA 
     2041 GGTTACTGCG GAACCTCACA GCCCTCCTGA TCCGAGCTAC GTACGGAGAA TATAGTACAG 
     2101 GGTACATTGA TAACGTGACC CTGGTTTCAG CCCGCCCTGT CTCTGGAGCC CCAGCCCCTT 
     2161 GGGTTGAACG TTGTGTATGC CCTGCTGGGT ACAAGGGACA ATTCTGCCAG GAATGTGCTT 
     2221 CTGGTTACAA AAGAGATTCG GCAAGATTGG GGCCTTTTGG CGCCTGTGTT CCCTGTAACT 
     2281 GCCAAGGGGG AGGGGCCTGT GATCCAGACA CGGGAGATTG CTACTCGGGG GACGAGAATC 
     2341 CTGACATTGA GTGTGCTGAC TGTCCCATCG GTTTCTACAA TGACCCACAT GACCCCCGCA 
     2401 GCTGCAAGCC ATGTCCCTGT CACAATGGGT TCAGCTGTTC AGTGATGCCT GAGACAGAGG 
     2461 AGGTGGTGTG TAACAACTGT CCCCCTGGGG TCACAGGTGC CCGCTGTGAG CTCTGTGCTG 
     2521 ATGGCTTCTT TGGGGATCCC TTTGGGGAAC GTGGCCCAGT GAGGCCTTGT CAACGCTGCC 
     2581 AATGCAACAA CAACGTGGAC CCCAATGCCT CTGGGAACTG TGACCAGTTG ACAGGCAGAT 
     2641 GCTTGAAATG TATCTACAAC ACGGCCGGTG TCTACTGTGA CCAGTGCAAA GCAGGTTACT 
     2701 TTGGAGACCC ATTGGCTCCC AACCCAGCAG ACAAGTGTCG AGCTTGCAAC TGCAGCCCCA 
     2761 TGGGCTCGGA GCCTGGAGAG TGTCGAGGTG ATGGCAGCTG TGTTTGCAAG CCAGGCTTTG 
     2821 GCGGCCTCAA CTGTGATCAC GCAGCCCTAA CCAGTTGTCC TGCTTGCTAC AATCAAGTGA 
     2881 AGATTCAGAT GGACCAGTTT ACCCAGCAGC TCCAGAGCCT GGAGGCCCTG GTTTCAAAGG 
     2941 CTCAGGGTGG TGGTGGTGGT GGTACAGTCC CCAGTGGGGA GCTGGAAGGC AGGATGCAGC 
     3001 AGGCTGAGCA GGCCCTTCGA GACATTCTAA GAGAAGCTCA GATTTCAGAA GGGGCAATGA 
     3061 GAGCCCTTGG CTTCCGGCTG GCCAAGGCAA GGAGCCAAGA GAACGACTAC AAGACCCGCC 
     3121 TGGATGACCT CAAGATGACT GCAGAAAGGA TCCGGGCCCT GGGCAGTCAG CATCAGAACA 
     3181 GAGTTCAGGA TACGAGCAGA CTCATCTCTC AGATGCGCCT GAGTCTGGCA GGAAGCGAAG 
     3241 CTTCCCTGCA AAACACTAAT ATCCATTCTT CTGAGCACTA CGTGGGGCCG AATGGATTTA 
     3301 AAAGTCTGGC TCAGGAGGCT ACAAGATTGG CAGACAGCCA CGCTGAGTCA GCTAACGCAA 
     3361 TGAAGCAACT AGCAAGGGAA ACTGAGGACT ACTCCAAACA AGCACTTTCA TTGGCCCGCA 
     3421 AGGCTCTGAG TGGAGGAGGC GGAAGTGGCG TCTTGGACAG CTCCGTGGTA CAAGGTCTTA 
     3481 TGGGAAAATT AGAGAAAACC AAGTCCCTGA GCCAGCAGCT GTCAAGGGAG GGCACCCAAG 
     3541 CCGACATTGA AGCTGATAGG TCGTATCAGC ACAGTCTCCG CCTCCTGGAT TCTGCCTCTC 
     3601 AGCTTCAGGG AGTCAGTGAT CTGTCCTTTC AGGTGGAAGC AAAGAGGATC AGACAAAAGG 
     3661 CTGATTCTCT CTCAAACCTG GTGACCAGAC AAACGGATGC ATTCACGCGT GTGCGAAACA 
     3721 ATCTGGGGAA CTGGGAAAAA GAAACACGGC AGCTTTTACA GACTGGAAAG GATAGGAGAC 
     3781 AGACTTCAGA TCAGCTGCTT TCCCGTGCCA ACCTTGCTAA AAACAGAGCC CAAGAAGCGC 
     3841 TAAGTATGGG CAATGCCACT TTTTATGAAG TTGAGAACAT CCTGAAGAAC CTCCGAGAGT 
     3901 TTGATCTGCA GGTTGAAGAC AGAAAAGCAG AGGCTGAAGA GGCCATGAAG AGACTCTCCT 
     3961 CTATTAGCCA GAAGGTTGCA GATGCCAGTG ACAAGACCCA GCAAGCAGAA ACGGCCCTGG 
     4021 GGAGCGCCAC TGCCGACACC CAACGGGCAA AGAACGCAGC TAGGGAGGCC CTGGAGATCA 
     4081 GCAGCGAGAT AGAGCAGGAG ATAGGGAGTC TGAACTTGGA AGCTAATGTG ACAGCAGATG 
     4141 GGGCCTTGGC CATGGAGAAG GGACTGGCCA CTCTGAAGAG CGAGATGAGA GAGATGGAGG 
     4201 GGGAGCTGGC CAGAAAGGAG CTGGAGTTTG ACACGGATAA GGACACGGTG CAGCTGGTGA 
     4261 TTACTGAAGC CCAGCAAGCT GATGCCAGAG CCACGAGTGC CGGAGTTACC ATCCAAGACA 
     4321 CACTCAACAC ATTGGACGGC ATCCTACACC TCATAGACCA GCCTGGCAGT GTGGATGAAG 
     4381 AAGGGATGAT GCTATTAGAA CAAGGGCTTT TCCAAGCCAA GACCCAGATC AACAGTCGAC 
     4441 TTCGGCCCTT GATGTCTGAC CTGGAGGAGA GGGTGCGTCG GCAGAGGAAC CACCTCCATC 
     4501 TGCTGGAGAC CAGCATAGAT GGAATTCTAG CTGATGTGAA GAACCTGGAG AACATTCGAG 
     4561 ACAACCTGCC CCCAGGCTGC TACAATACCC AAGCTCTTGA GCAACAGAGC GGACCGACGC 
     4621 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4681 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5521 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6241 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6301 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6361 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6421 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6481 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6541 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6601 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6661 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6721 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6781 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6841 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6901 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6961 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7021 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7081 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7141 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7201 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7261 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7321 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7381 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7441 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7501 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7561 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7621 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7681 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7921 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7981 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8041 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8101 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8161 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8221 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8281 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8341 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8401 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8461 GCGAATTTT
//