LOCUS       MR222690L3                8978 bp    DNA     circular  SYN 
DEFINITION  Eng (Myc-DDK-tagged) - Mouse endoglin (Eng), transcript variant 2
ACCESSION   NM_001146350
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7269..7449 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8177..8836 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7497..8116 
                     /label="ColE1"  
     misc_feature    5309..5332 
                     /label="DDK"  
     misc_feature    6144..6164 
                     /label="LR50"  
     misc_feature    5261..5332 
                     /label="MYC"  
     misc_feature    5342..5407 
                     /label="P2A"  
     misc_feature    5408..6007 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6153..6744 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5243..5249 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5243 
                     /label="Eng"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGACCGTGGC GTGCTCCCTC TGCCCATTAC 
     3361 CCTGCTGTTT GTCATCTATA GCTTTGTACC CACAACAGGT CTCGCAGAAA GAGTCGGCTG 
     3421 TGATCTACAG CCTGTGGACC CCACAAGGGG TGAGGTGACG TTTACCACCA GCCAGGTCTC 
     3481 CGAGGGCTGT GTAGCTCAGG CTGCCAATGC TGTGCGTGAA GTCCACGTTC TCTTCCTGGA 
     3541 TTTTCCCGGA ATGCTGTCAC ATCTGGAGCT GACTCTTCAG GCATCCAAGC AAAATGGCAC 
     3601 GGAGACCCAG GAGGTGTTCC TGGTCCTCGT TTCGAACAAA AATGTCTTCG TGAAGTTCCA 
     3661 GGCCCCGGAA ATCCCATTGC ACTTGGCCTA CGACTCCAGC CTGGTCATCT TCCAAGGACA 
     3721 GCCAAGAGTC AACATCACAG TGCTACCATC CCTTACCTCC AGGAAACAGA TCCTCGACTG 
     3781 GGCAGCCACC AAGGGCGCCA TCACCTCGAT AGCAGCACTG GATGACCCCC AAAGCATCGT 
     3841 CCTCCAGTTG GGCCAAGACC CAAAGGCACC ATTCTTGTGC TTGCCAGAAG CTCACAAGGA 
     3901 CATGGGCGCC ACACTTGAAT GGCAACCACG AGCCCAGACC CCAGTCCAAA GCTGTCGCTT 
     3961 GGAAGGTGTG TCTGGCCACA AGGAGGCCTA CATCCTGAGG ATCCTGCCAG GTTCTGAGGC 
     4021 CGGGCCCCGG ACGGTGACCG TAATGATGGA ACTGAGTTGC ACATCTGGGG ACGCCATTCT 
     4081 CATCCTGCAT GGTCCTCCAT ATGTCTCCTG GTTCATCGAC ATCAACCACA GCATGCAGAT 
     4141 CTTGACCACA GGTGAATACT CCGTCAAGAT CTTTCCAGGA AGCAAGGTCA AAGGCGTGGA 
     4201 GCTCCCAGAC ACACCCCAAG GCCTGATAGC GGAGGCCCGC AAGCTCAATG CCAGCATTGT 
     4261 CACCTCCTTT GTAGAGCTCC CTCTGGTCAG CAATGTCTCC CTGAGGGCCT CCAGCTGCGG 
     4321 TGGTGTGTTC CAGACCACCC CTGCACCCGT TGTGACCACA CCTCCCAAGG ACACATGCAG 
     4381 CCCCGTGCTA CTCATGTCCC TGATCCAGCC AAAGTGTGGC AATCAGGTCA TGACTCTGGC 
     4441 ACTCAATAAA AAACACGTGC AGACTCTCCA GTGCACCATC ACAGGCCTGA CTTTCTGGGA 
     4501 CTCCAGCTGC CAGGCTGAAG ACACTGACGA CCATCTTGTC CTGAGTAGCG CCTACTCCAG 
     4561 CTGCGGCATG AAAGTGACAG CCCATGTGGT CAGCAATGAG GTGATCATCA GTTTCCCGTC 
     4621 AGGCTCACCA CCACTTCGGA AAAAGGTACA GTGCATCGAC ATGGACAGCC TCTCCTTCCA 
     4681 GCTGGGCCTC TACCTCAGCC CGCACTTCCT CCAGGCATCC AACACCATCG AACTAGGCCA 
     4741 GCAGGCCTTC GTACAGGTGA GCGTGTCTCC ATTGACCTCT GAGGTCACAG TCCAGCTAGA 
     4801 TAGCTGCCAT CTGGACTTGG GGCCCGAAGG GGACATGGTG GAACTCATCC AGAGCCGAAC 
     4861 AGCCAAGGGC AGCTGTGTGA CCTTGCTGTC TCCAAGCCCT GAAGGTGACC CACGCTTCAG 
     4921 CTTCCTCCTC CGGGTCTACA TGGTGCCCAC ACCCACCGCT GGCACCCTCA GTTGCAACTT 
     4981 AGCTCTGCGC CCTAGCACCT TGTCCCAGGA AGTCTACAAG ACAGTCTCCA TGCGCCTGAA 
     5041 CATCGTCAGC CCTGACCTGT CTGGTAAAGG CCTTGTCCTG CCCTCTGTAC TGGGTATCAC 
     5101 CTTTGGTGCC TTCCTGATTG GGGCCCTGCT CACAGCTGCA CTCTGGTACA TCTATTCTCA 
     5161 CACACGTGAG TATCCCAAGC CTCCACCCCA TTCCCACAGC AAGCGCTCAG GGCCCGTCCA 
     5221 CACCACCCCG GGGCACACCC AGTGGAGCCT CACGCGTACG CGGCCGCTCG AGCAGAAACT 
     5281 CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA 
     5341 GGTTTGGGTA GGAAGCGGAG CTACTAACTT CAGCCTGCTG AAGCAGGCTG GAGACGTGGA 
     5401 GGAGAACCCT GGACCTATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA 
     5461 CGTCCCCAGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA 
     5521 CACCGTCGAT CCGGATCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC 
     5581 GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT 
     5641 CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT 
     5701 GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC 
     5761 GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA 
     5821 GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG 
     5881 GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG 
     5941 CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT GCATGACCCG 
     6001 CAAGCCCGGT GCCTGATGTA CACCCAAACG GCCGGCCGCG GTCTGTACAA GTAGGATTCG 
     6061 TCGAGGGACC TAATAACTTC GTATAGCATA CATTATACGA AGTTATACAT GTTTAAGGGT 
     6121 TCCGGTTCCA CTAGGTACAA TTCGATATCA AGCTTATCGA TAATCAACCT CTGGATTACA 
     6181 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 
     6241 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 
     6301 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 
     6361 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 
     6421 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 
     6481 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 
     6541 TGGTGTTGTC GGGGAAATCA TCGTCCTTTC CTTGGCTGCT CGCCTGTGTT GCCACCTGGA 
     6601 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 
     6661 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 
     6721 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC ATCGATACCG TCGACCTCGA TCGAGACCTA 
     6781 GAAAAACATG GAGCAATCAC AAGTAGCAAT ACAGCAGCTA CCAATGCTGA TTGTGCCTGG 
     6841 CTAGAAGCAC AAGAGGAGGA GGAGGTGGGT TTTCCAGTCA CACCTCAGGT ACCTTTAAGA 
     6901 CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG 
     6961 GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATATCCTTG ATCTGTGGAT CTACCACACA 
     7021 CAAGGCTACT TCCCTGATTG GCAGAACTAC ACACCAGGGC CAGGGATCAG ATATCCACTG 
     7081 ACCTTTGGAT GGTGCTACAA GCTAGTACCA GTTGAGCAAG AGAAGGTAGA AGAAGCCAAT 
     7141 GAAGGAGAGA ACACCCGCTT GTTACACCCT GTGAGCCTGC ATGGGATGGA TGACCCGGAG 
     7201 AGAGAAGTAT TAGAGTGGAG GTTTGACAGC CGCCTAGCAT TTCATCACAT GGCCCGAGAG 
     7261 CTGCATCCGG ACTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT 
     7321 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA 
     7381 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA 
     7441 GTGTGGAAAA TCTCTAGCAG CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 
     7501 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT 
     7561 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC 
     7621 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC 
     7681 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT 
     7741 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 
     7801 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG 
     7861 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA 
     7921 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC 
     7981 GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA 
     8041 ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 
     8101 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC 
     8161 TCACGTTAAG GGATTTTGGT CATGATTACG CCCCGCCCTG CCACTCATCG CAGTACTGTT 
     8221 GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA AACGGCATGA TGAACCTGAA 
     8281 TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA TTTGCCCATG GTGAAAACGG 
     8341 GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA ACTGGTGAAA CTCACCCAGG 
     8401 GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT AGGGAAATAG GCCAGGTTTT 
     8461 CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT 
     8521 ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG GAAAACGGTG TAACAAGGGT 
     8581 GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC CATACGGAAC TCCGGATGAG 
     8641 CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA AAACTTGTGC TTATTTTTCT 
     8701 TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT CTGGTTATAG GTACATTGAG 
     8761 CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA TTGGGATATA TCAACGGTGG 
     8821 TATATCCAGT GATTTTTTTC TCCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     8881 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     8941 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGAC
//