LOCUS       MR222649                8418 bp    DNA     circular  SYN 
DEFINITION  Itga1 (Myc-DDK-tagged) - Mouse integrin alpha 1 (Itga1)
ACCESSION   NM_001033228
VERSION     NM_001033228.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4575..4666 
                     /label="Myc-DDK"  
     misc_feature    4696..5281 
                     /label="PolyA signal"  
     misc_feature    4753..4772 
                     /label="Restriction Enzyme"  
     misc_feature    5557..6229 
                     /label="ColE1"  
     misc_feature    6659..7453 
                     /label="Kan/Neo"  
     misc_feature    7488..7790 
                     /label="SV40 ori"  
     misc_feature    7876..8353 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4557..4563 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4557 
                     /label="Itga1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCCCAGG CGTCCTGCTA GCCTAGAGGT CACTGTAGCC TGCATCTGGC 
     1081 TTCTCACCGT TATCCTAGGC GTCTGCATCT CCTTCAACGT TGATGTCAAA AACTCCATGA 
     1141 GCTTCAGTGG TCCAGTGGAG GACATGTTTG GATACACCGT TCAACAGTAT GAAAACGAAG 
     1201 AAGGCAAATG GGTTCTTATT GGTTCTCCTT TAGTTGGCCA ACCCAAAGCA AGAACTGGAG 
     1261 ATGTCTATAA GTGTCCAGTT GGGAGAGAGA GATCAATGCC TTGTGTGAAG TTGGATTTAC 
     1321 CAGTTAACAC ATCGATTCCT AATGTCACAG AAATAAAGGA AAACATGACA TTTGGATCAA 
     1381 CTTTAGTCAC CAATCCGAAG GGAGGATTTC TGGCATGTGG GCCCTTGTAT GCCTATAGAT 
     1441 GTGGACATTT GCATTATACG ACTGGCATAT GTTCTGATGT CAGCCCTACA TTTCAAGTTG 
     1501 TGAACTCGTT CGCCCCTGTA CAAGAATGTA GCACACAGCT TGACATAGTC ATCGTCCTAG 
     1561 ATGGCTCCAA CAGCATCTAC CCCTGGGAAA GTGTCACTGC CTTTTTAAAC GACCTTCTTA 
     1621 AGAGGATGGA CATTGGCCCC AAACAGACAC AGGTTGGAAT TGTACAATAT GGAGCAAATG 
     1681 TAACACATGA GTTCAACCTC AATAAGTATT CATCCACAGA AGAGGTCCTG GTGGCAGCAA 
     1741 ACAAAATAGG CCGCAGGGGT GGTCTCCAGA CGATGACAGC CCTTGGAATA GACACAGCCA 
     1801 GGAAAGAGGC ATTCACTGAA GCTCGGGGGG CCAGAAGGGG AGTGAAAAAA GTCATGGTTA 
     1861 TTGTGACTGA TGGAGAATCG CATGACAACT ATCGCCTGAA ACAGGTCATC CAAGACTGTG 
     1921 AGGATGAAAA CATTCAGCGA TTTTCCATAG CTATCCTTGG CCACTATAAC AGGGGGAACT 
     1981 TAAGCACCGA AAAATTTGTG GAGGAGATAA AATCAATCGC AAGTGAGCCC ACCGAAAAGC 
     2041 ACTTCTTCAA CGTCTCAGAT GAGTTGGCCC TGGTCACTAT TGTTAAAGCT CTGGGAGAAA 
     2101 GGATATTTGC TTTGGAAGCT ACAGCTGACC AGTCAGCAGC TTCGTTTGAA ATGGAGATGT 
     2161 CTCAGACCGG CTTCAGTGCT CATTATTCAC AGGACTGGGT CATGCTTGGA GCTGTTGGAG 
     2221 CCTATGACTG GAACGGAACT GTGGTCATGC AGAAGGCTAA CCAGATTGTC ATCCCTCATA 
     2281 ACACCACCTT TCAAACTGAG CCCACCAAGA TGAACGAGCC TCTTGCTTCT TACTTGGGTT 
     2341 ACACAGTGAA CTCTGCCACC ATCCCGGGAG ATGTGCTCTA TATCGCTGGG CAGCCTCGGT 
     2401 ACAATCACAC CGGCCAGGTC GTCATCTACA AGATGGAGGA TGGGGACGTC AACATTCTGC 
     2461 AGACACTCAG TGGAGAGCAG ATCGGATCCT ACTTTGGTAG CGTCTTAACC ACAATTGACA 
     2521 TCGACAAAGA TTCTTACACT GATCTGCTTC TCGTCGGGGC TCCCATGTAT ATGGGGACAG 
     2581 AGAAAGAGGA ACAGGGCAAG GTGTACGTGT ACGCTGTGAA TCAGACGAGG TTTGAATATC 
     2641 AAATGAGCCT GGAACCAATT AAGCAGACCT GCTGTTCATC CCTGAAGGAT AATTCATGCA 
     2701 CAAAAGAAAA CAAGAATGAG CCCTGTGGGG CCCGTTTTGG AACAGCAGTT GCTGCTGTAA 
     2761 AAGACCTCAA CGTGGATGGC TTTAATGACG TCGTGATTGG AGCTCCCCTG GAAGACGACC 
     2821 ACGCAGGAGC TGTGTACATT TATCATGGCA GTGGCAAGAC CATAAGGAAA GAGTATGCGC 
     2881 AACGCATTCC CTCAGGTGGG GATGGGAAGA CGCTGAAATT TTTTGGCCAG TCTATCCACG 
     2941 GAGAGATGGA TTTAAATGGC GATGGTCTGA CTGACGTGAC CATTGGAGGC CTTGGAGGAG 
     3001 CTGCCCTCTT CTGGGCCAGA GATGTGGCTG TAGTTAAAGT GACCATGAAT TTTGAACCCA 
     3061 ACAAAGTGAA TATTCAAAAG AAAAACTGCC GTGTGGAGGG CAAAGAAACA GTATGCATAA 
     3121 ATGCTACAAT GTGTTTTCAT GTGAAATTGA AGTCTAAAGA AGACTCAGTT TACGAGGCTG 
     3181 ATCTGCAGTA CCGTGTCACC CTTGATTCGC TGAGGCAGAT ATCACGGAGC TTTTTTTCTG 
     3241 GAACTCAGGA AAGGAGGATT CAAAGAAACC TCACCGTTCG AGAATCCGAA TGCATCAGGC 
     3301 ACTCCTTCTA CATGTTGGAT AAGCACGACT TTCAGGACTC CGTGAGAGTG ACTTTGGATT 
     3361 TTAACCTCAC TGATCCAGAA AATGGGCCCG TGCTCGATGA CGCTCTGCCA AACTCAGTCC 
     3421 ATGGACATAT TCCTTTTGCC AAAGACTGTG GGAACAAGGA AAGATGCGTT TCAGACCTCA 
     3481 CCCTGGATGT GTCCACAACA GAAAAGAACC TGCTGATTGT CAGATCCCAG AATGACAAGT 
     3541 TCAATGTCAG CCTCACCGTC AAAAACAAGG GAGACAGTGC GTACAACACC CGGACAGTGG 
     3601 TTCAGTATTC TCCAAATCTG ATTTTTTCAG GAATTGAGGA GATCCAAAAA GATAGCTGCG 
     3661 AATCCAATCA AAATATCACG TGTAGAGTTG GATATCCTTT CCTGAGGACA GGAGACGTGG 
     3721 TTAACTTCAA AATAATATTC CAGTTTAATA CATCACATCT CTCAGAAAAT GCAATTATTC 
     3781 ATTTAAGTGC AACAAGTGAC AGTGAAGAGC CCCTGGAATC TCTTTACGAT AATGAAGTAA 
     3841 ATATTTCCAT CCCCGTAAAA TATGAAGTCG GACTGCAGTT TTACAGTTCT GCGAGTGAAC 
     3901 ACCACATTTC AGTTGCTGCC AATGAGACTG TTCCTGAGCT GATTAATTCC ACCAAGGACA 
     3961 TTGGGGATGA AATTAATGTC TTCTACACGA TTAGAAAAAG AGGGCATTTC CCAATGCCAG 
     4021 AACTTCGGCT GGCAATTTCA TTTCCCAATC TGACGTCAGA TGGCTATCCT GTACTGTACC 
     4081 CAACTGGATG GTCATCTTCT GATAATGTAA ACTGCAGACC TCGAAGCCTT GAGGACCCTT 
     4141 TGGGTATCAA CTCTGGGAAG AAAATGACAA TATCAAAGTC TGAGGTTCTC AAAAGAGGCA 
     4201 CAATCCAGGA CTGCAGTACC TGCAAGATTG CTACCATCAC GTGTCATCTC CTTCCCTCGG 
     4261 ATGTGAGTCA AGTGAATGTC TCACTCATCT TGTGGAAACC AACTTTCATA AAAGCACATT 
     4321 TTTCCAGCTT AAATCTTACC ATACGGGGAG AACTTCAGAG TGAAAACTCA TCGCTGACTT 
     4381 TAAGCAGCAG CAACCGGAAA CGAGAGCTGG CTATTCAGAT ATCCAAAGAC GGGCTCCCAG 
     4441 GCCGAGTGCC GCTGTGGGTT ATCCTCCTGA GCGCCTTTGC GGGACTCCTG CTGTTAATGC 
     4501 TGCTTATATT AGCTCTGTGG AAGATTGGAT TCTTCAAAAG GCCACTAAAG AAGAAAATGG 
     4561 AGAAAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4621 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4681 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4741 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4801 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4861 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4921 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4981 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5041 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5101 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5161 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5221 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5281 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5341 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5401 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5461 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5521 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5581 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5641 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5701 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5761 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5821 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5881 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5941 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6001 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6061 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6121 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6181 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6241 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6301 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6361 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6421 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6481 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6541 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6601 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6661 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6721 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6781 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6841 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6901 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6961 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7021 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7081 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7141 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7201 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7261 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7321 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7381 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7441 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7501 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7561 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7621 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7681 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7741 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7801 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7861 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7921 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7981 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8041 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8101 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8161 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8221 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8281 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8341 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8401 AAATTTAACG CGAATTTT
//