LOCUS       MR222635                7893 bp    DNA     circular  SYN 
DEFINITION  Ppp1r12a (Myc-DDK-tagged) - Mouse protein phosphatase 1, regulatory (inhibitor) subunit 12A (Ppp1r12a)
ACCESSION   NM_027892
VERSION     NM_027892.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4050..4141 
                     /label="Myc-DDK"  
     misc_feature    4171..4756 
                     /label="PolyA signal"  
     misc_feature    4228..4247 
                     /label="Restriction Enzyme"  
     misc_feature    5032..5704 
                     /label="ColE1"  
     misc_feature    6134..6928 
                     /label="Kan/Neo"  
     misc_feature    6963..7265 
                     /label="SV40 ori"  
     misc_feature    7351..7828 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4032..4038 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4032 
                     /label="Ppp1r12a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGATGGCG GACGCGAAGC AGAAGCGGAA CGAGCAGCTG AAGCGCTGGA 
     1081 TCGGCTCCGA GACGGACCTC GAGCCGCCCG TGGTGAAGCG CCAGAAGACC AAGGTGAAGT 
     1141 TCGACGATGG CGCCGTCTTC CTCGCCGCCT GCTCCAGCGG CGACACGGAC GAGGTCCTCA 
     1201 AGCTGCTGCA CCGCGGCGCC GACATCAATT ACGCCAATGT GGACGGACTC ACCGCCCTGC 
     1261 ACCAGGCTTG CATTGATGAC AATGTTGATA TGGTGAAGTT TCTGGTAGAA AATGGAGCAA 
     1321 ATATCAATCA ACCTGACAAT GAAGGCTGGA TACCACTCCA TGCAGCTGCT TCCTGTGGAT 
     1381 ATCTCGATAT TGCAGAGTTT TTGATTGGTC AAGGAGCACA TGTAGGAGCT GTCAACAGTG 
     1441 AAGGAGACAC ACCTTTAGAT ATTGCAGAGG AGGAGGCGAT GGAAGAGCTA CTTCAAAATG 
     1501 AGGTTAATCG GCAAGGTGTT GATATAGAAG CGGCTCGGAA AGAGGAGGAG CGGGTAATGC 
     1561 TAAGAGACGC GAGGCAGTGG TTAAACAGCG GCCACATCAG TGACGTCCGG CATGCAAAGT 
     1621 CCGGGGGCAC AGCACTCCAC GTGGCGGCCG CCAAAGGCTA TACAGAAGTG TTAAAACTTT 
     1681 TAATACAGGC AGGCTATGAT GTTAATATTA AAGATTATGA TGGCTGGACA CCTCTTCATG 
     1741 CTGCAGCTCA TTGGGGTAAA GAAGAAGCAT GTCGGATTTT AGTGGACAAT CTGTGTGATA 
     1801 TGGAGACGGT CAACAAAGTG GGCCAAACAG CCTTTGATGT AGCAGATGAA GACATTTTGG 
     1861 GATATCTAGA AGAGTTGCAA AAAAAACAAA CTCTGCTCCA TAGTGAGAAG CGGGATAAGA 
     1921 AATCTCCACT GATCGAGTCC ACAGCAAATA TGGAAAACAA TCAACCACAG AAGGCTTTTA 
     1981 AAAACAAGGA AACGTTGATT ATTGAGCCAG AGAAAAATGC ATCTCGCATC GAGTCTCTGG 
     2041 AGCATGAGAA GGCTGATGAG GAGGAAGAAG GCAAGAAGGA CGAGTCGAGC TGTTCCAGTG 
     2101 AGGAGGACGA GGAGGACGAT TCAGAGTCAG AGGCAGAGAC AGATAAGACA AAGCCCATGG 
     2161 CTTCTGTAAG CAATGCTCAT ACTTCCAGCA CGCAGGCAGC CCCTGCAGCT GTTACAGCAC 
     2221 CTACTCTGTC TTCCAACCAG GGGACCCCTA CATCACCTGT TAAAAAGTTT CCAATATCGA 
     2281 CTACAAAAAT TTCTCCCAAA GAAGAGGAAA GAAAAGATGA GTCTCCTGCA TCCTGGAGGT 
     2341 TAGGGCTTCG AAAGACTGGC AGTTACGGTG CCCTGGCTGA AATCAGTGCG TCTAAAGAAG 
     2401 CCCAGAAGGA GAAAGACACT GCAGGGGTGA TGCGGTCGGC TTCCAGTCCG AGACTCTCGT 
     2461 CCTCTTTGGA TAATAAAGAA AAGGAGAAAG ATAACAAAGG AACGAGACTT GCGTATGTCA 
     2521 CCCCTACAAT CCCGAGACGA CTAGCCAGTA CATCTGACAT TGAAGAGAAG GAGAACAGAG 
     2581 AGTCTTCAAG TTTGCGAACA AGTAGTTCTT ACACAAGAAG AAAATGGGAA GATGATCTTA 
     2641 AAAAGAATAG TTCGATCAAT GAAGGATCCA CTTACCATAG AAGTTGCTCC TTTGGTAGAA 
     2701 GACAAGATGA TTTGATTAGT TGTAGTGTTC CAAGCACCAC ATCAACACCA ACAGTTACCT 
     2761 CTGCAGCGGG GCTTCAGAGA AGCCTCCCTT CCAGCACAAG CACTGCTGCA AAGACTCCCC 
     2821 CGGGTTCCTC CTCCGCAGGC ACACAGAGCA GTACCTCAAA TCGTTTGTGG GCTGAGGATA 
     2881 GTACTGAGAA AGAAAAGGAC AGTGCTCCTA CTGCAGTGAC CATTCCTGTG GCTCCAACTG 
     2941 TTGTAAATGC TGCAGCTCCT TCCACCACCA CCCTGACTAC AACTACTGCT GGCACTGTTT 
     3001 CCGAGGTCAG GGAGAGACGC AGGTCATACC TCACTCCTGT TAGGGACGAA GAGTCTGAAT 
     3061 CCCAAAGAAA AGCAAGATCT AGACAAGCAA GACAGTCTAG ACGGTCAACA CAGGGGGTAA 
     3121 CACTGACTGA TCTTCAGGAA GCTGAAAAAA CAATAGGAAG AAGTCGTTCT ACGAGAACCA 
     3181 GAGAACAAGA AAATGAAGAG AAAGAAAAAG AAGAAAAGGA AAAGCAGGAT AAAGAGAAAC 
     3241 AAGAAGAAAA GAAGGAGTCA GAAGCATCTA GAGAAGATGA ATATAAACAA AAGTATTCAA 
     3301 GAACATACGA TGAGACTTAC ACACGTTATA GACCTGTGTC AACTTCAAGT TCAAGCGCTC 
     3361 CGTCGTCGTC CTCACTCTCT ACTCTAGGCA GCACACTCTA TGCCTCAAGT CAGCTCAACA 
     3421 GGCCAAACAG TCTTGTAGGT ATAACCTCTG CCTACTCCCG GGGATTAGCC AAAGAAAACG 
     3481 AGAGAGAGGG AGAGAAAAAA GAAGAGGAGA AAGAAGGGGA AGATAAGTCA CAGCCTAAGT 
     3541 CAATCAGAGA ACGACGGCGA CCCAGAGAAA AGCGACGGTC TACTGGAGTC TCCTTCTGGA 
     3601 CACAAGATAG TGATGAAAAT GAGCAAGAGC GGCAATCGGA CACTGAGGAC GGCTCCAGCA 
     3661 AGAGAGAGAC GCAGACGGAT TCTGTTTCCA GGTATGACAG CAGTTCCACA TCATCAAGTG 
     3721 ATCGGTATGA TTCCTTGCTG GGTCGCTCTG CTTCATACAG TTACTTAGAA GACAGAAAAC 
     3781 CTTACAGTAG CAGGCTAGAA AAGGATGACT CAACTGACTT CAAAAAGCTT TATGAACAAA 
     3841 TTTTAGCTGA AAATGAAAAG CTAAAAGCAC AGCTACATGA TACAAATATG GAACTAACGG 
     3901 ATCTAAAGTT GCAGTTGGAA AAGGCTACCC AGAGACAAGA ACGATTTGCT GACAGGTCAC 
     3961 AATTGGAGAT GGAAAAAAGG GTGGCCGGCA AGAGTCAGTA TCTTCTGGGC GGAACGAAGA 
     4021 GCTCTAGAAA GAAGAATATC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4081 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4141 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4201 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4261 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4321 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4381 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4441 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4501 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4561 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4621 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4681 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4741 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4801 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4861 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4921 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4981 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5041 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5101 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5161 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5221 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5281 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5341 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5401 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5461 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5521 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5581 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5641 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5701 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5761 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5821 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5881 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5941 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6001 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6061 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6121 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6181 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6241 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6301 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6361 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6421 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6481 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6541 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6601 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6661 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6721 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6781 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6841 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6901 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6961 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7021 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7081 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7141 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7201 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7261 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7321 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7381 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7441 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7501 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7561 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7621 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7681 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7741 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7801 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7861 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//