LOCUS       MR222629                7545 bp    DNA     circular  SYN 
DEFINITION  Ltk (Myc-DDK-tagged) - Mouse leukocyte tyrosine kinase (Ltk), transcript variant 4
ACCESSION   NM_203345
VERSION     NM_203345.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3702..3793 
                     /label="Myc-DDK"  
     misc_feature    3823..4408 
                     /label="PolyA signal"  
     misc_feature    3880..3899 
                     /label="Restriction Enzyme"  
     misc_feature    4684..5356 
                     /label="ColE1"  
     misc_feature    5786..6580 
                     /label="Kan/Neo"  
     misc_feature    6615..6917 
                     /label="SV40 ori"  
     misc_feature    7003..7480 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3684..3690 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3684 
                     /label="Ltk"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTGCTCC CACCGGCTGC TGCTGTGGCT GGGAGCTGCT GGAACTATTC 
     1081 TTTGCTCCAA CTCGGAGTTC CAGACACCTT TTCTAACACC TTCGCTTTTG CCGGTGCTGG 
     1141 TACTCAATTC CCAGGAGCAG AAAGTCACCC CCACACCCAG TAAATTGGAG CCAGCTTCCC 
     1201 TCCCAAATCC TCTAGGCACA CGGGGGCCTT GGGTGTTTAA CACCTGTGGC GCCAGCGGCA 
     1261 GGAGCGGACC CACACAAACA CAGTGCGATG GGGCATACAC AGGAAGCAGC GTGATGGTGA 
     1321 CAGTGGGGGC TGCCGGGCCG CTCAAAGGCG TGCAGCTGTG GCGGGTGCCA GACACAGGCC 
     1381 AGTATCTGAT CTCCGCCTAC GGAGCGGCGG GCGGCAAGGG CGCCCAAAAC CACCTGTCAC 
     1441 GGGCGCACGG CATCTTCCTC TCAGCAGTCT TCTTCCTCCG TCGCGGGGAG CCGGTGTACA 
     1501 TCCTTGTGGG GCAGCAGGGC CAGGACGCCT GTCCCGGAGG GAGCCCTGAG AGCCAACTCG 
     1561 TCTGTCTGGG AGAGTCTGGG GAGCATGCAA CCACCTATGG GACCGAAAGG ATCCCAGGCT 
     1621 GGAGACGCTG GGCCGGCGGG GGCGGGGGTG GCGGAGGCGC CACCTCCATC TTCCGGCTGC 
     1681 GCGCGGGGGA GCCAGAGCCG CTGCTGGTGG CGGCGGGAGG CGGCGGGAGG TCCTACCGGA 
     1741 GGCGACCTGA CCGCGGCCGG ACTCAGGCCG TCCCCGAGAG GCTGGAGACC CGCGCGGCGG 
     1801 CGCCGGGCAG CGGCGGGAGA GGAGGCGCGG CAGGTGGAGG GAGCGGCTGG ACGTCGCGAG 
     1861 CCCACTCTCC GCAGGCCGGA CGCTCGCCGC GGGAAGGGGC CGAGGGCGGC GAGGGCTGCG 
     1921 CGGAGGCCTG GGCTGCGCTC CGCTGGGCTG CGGCGGGAGG CTTCGGGGGC GGCGGCGGGG 
     1981 CCTGCGCGGC GGGCGGCGGT GGCGGCGGCT ACCGGGGCGG TGATACTTCT GAGTCTGACC 
     2041 TCCTCTGGGC TGATGGGGAA GATGGCACAT CCTTTGTCCA CCCCAGTGGT GAGCTCTACC 
     2101 TACAGCCTCT GGCAGTCACA GAGGGCCATG GGGAGGTGGA GATCCGAAAG CATCCCAACT 
     2161 GCAGTCACTG CCCTTTCAAA GACTGCCAGT GGCAGGCAGA GCTCTGGACG GCCGAATGCA 
     2221 CGTGCCCAGA GGGCACGGAG CTAGCTGTGG ATAATGTCAC TTGCATGGAC CTGCCAACCA 
     2281 CCGCAAGCCC TCTGATCCTG ATGGGAGCTG TAGTGGCAGC CTTGGCACTG AGTCTCCTAA 
     2341 TGATGTGTGC AGTCCTGATT CTAGTGAACC AGAAGTGTCA GGGCCTGTGG GGGACCAGGC 
     2401 TGCCAGGCCC TGAGCTTGAG CTAAGCAAGC TTCGATCCTC TGCCATCAGG ACAGCACCCA 
     2461 ACCCTTACTA TTGTCAGGTG GGACTCAGTC CTGCCCAGCC CTGGCCTTTG CCCCCAGGGC 
     2521 TCACTGAGGT TTCACCAGCC AATGTCACTC TACTCAGAGC CCTTGGCCAT GGTGCCTTTG 
     2581 GGGAAGTGTA CGAGGGACTA GTGACTGGTC TTCCTGGGGA CTCCAGTCCT CTTCCAGTGG 
     2641 CTATTAAGAC TCTGCCAGAG CTCTGCTCCC ATCAGGATGA GCTGGATTTT CTCATGGAGG 
     2701 CTCTGATCAT CAGCAAGTTC AGCCATCAGA ACATTGTACG CTGTGTGGGG CTCAGCTTTC 
     2761 GGTCTGCCCC GCGCCTCATT CTGCTGGAGC TGATGTCTGG TGGGGACATG AAGAGCTTTT 
     2821 TGAGGCACAG CAGACCACAC CCAGGACAAC TGGCACCTCT GACCATGCAG GACCTATTGC 
     2881 AGCTGGCCCA GGATATAGCC CAGGGCTGCC ACTACCTGGA GGAAAATCAC TTCATTCACA 
     2941 GAGACATTGC TGCCCGTAAC TGTCTGCTTA GCTGCAGTGG AGCCAGCCGA GTGGCCAAGA 
     3001 TTGGAGATTT TGGAATGGCA AGAGATATCT ACCAGGCCAG TTATTATCGC AAGGGTGGCC 
     3061 GGACCTTGCT CCCAGTCAAG TGGATGCCGC CAGAAGCTCT CCTGGAGGGC CTTTTCACAT 
     3121 CCAAGACAGA CTCCTGGTCT TTTGGGGTCC TGCTCTGGGA GATCTTCTCA CTGGGGTATA 
     3181 TGCCCTACCC TGGACATACC AACCAGGAGG TTCTAGACTT CATTGCCACA GGGAACAGGA 
     3241 TGGACCCTCC TAGGAACTGT CCTGGGCCAG TGTACCGAAT CATGACCCAG TGTTGGCAGC 
     3301 ATCAGCCGGA GCTCCGCCCT GACTTTGGCA GCATCTTGGA ACGGATTCAG TACTGCACTC 
     3361 AGGACCCTGA TGTGCTGAAC TCACCCCTGC CCGTGGAACC TGGGCCCATT CTAGAGGAGG 
     3421 AAGAGGCCTC CAGGCTGGGA AACAGGTCAC TGGAGGGTCT TAGATCCCCA AAGCCCCTAG 
     3481 AGCTGAGTTC TCAGAACTTG AAGAGCTGGG GAGGAGGCCT TCTTGGCTCT TGGCTGCCCT 
     3541 CTGGCCTCAA GACCCTCAAA CCCAGGTGCC TCCAACCTCA GAACATTTGG AACCCCACCT 
     3601 ATGGCTCCTG GACCCCAAGG GGCCCCCAGG GTGAAGATAC AGGCATTGAA CACTGCAATG 
     3661 GCTCCTCCTC AAGTTCCATT CCAGGCATCC AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3721 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3781 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3841 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3901 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     3961 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4021 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4081 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4141 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4201 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4261 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4321 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4381 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4441 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4501 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4561 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4621 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4681 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4741 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4801 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4861 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4921 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     4981 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5041 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5101 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5161 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5221 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5281 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5341 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5401 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5461 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5521 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5581 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5641 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5701 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5761 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5821 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5881 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     5941 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6001 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6061 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6121 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6181 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6241 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6301 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6361 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6421 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6481 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6541 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6601 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6661 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6721 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6781 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6841 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6901 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     6961 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7021 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7081 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7141 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7201 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7261 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7321 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7381 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7441 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7501 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//