LOCUS       MR222625                8397 bp    DNA     circular  SYN 
DEFINITION  Ehmt2 (Myc-DDK-tagged) - Mouse euchromatic histone lysine N-methyltransferase 2 (Ehmt2), transcript variant short
ACCESSION   NM_147151
VERSION     NM_147151.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4554..4645 
                     /label="Myc-DDK"  
     misc_feature    4675..5260 
                     /label="PolyA signal"  
     misc_feature    4732..4751 
                     /label="Restriction Enzyme"  
     misc_feature    5536..6208 
                     /label="ColE1"  
     misc_feature    6638..7432 
                     /label="Kan/Neo"  
     misc_feature    7467..7769 
                     /label="SV40 ori"  
     misc_feature    7855..8332 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4536..4542 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4536 
                     /label="Ehmt2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGGCG GCGGGAGCTG CTGCGGCGGC GGCCGCCGAG GGGGAGGCCC 
     1081 CCGCTGAGAT GGGGGCGCTG CTGCTGGAGA AGGAGCCCCG AGGAGCCGCC GAGAGAGTTC 
     1141 ATAGCTCTTT GGGGGACACC CCTCAGAGTG AGGAGACCCT TCCCAAGGCC AACCCCGACT 
     1201 CCTTGGAGCC TGCCGGCCCC TCCTCTCCGG CCTCTGTCAC TGTCACCGTC GGCGATGAGG 
     1261 GGGCTGACAC CCCTGTCGGG GCCGCATCAC TCATCGGGGA CGAACCCGAG AGCCTGGAGG 
     1321 GAGATGGGGG TCGCATCGTG CTGGGCCATG CCACAAAGTC GTTCCCCTCT TCCCCCAGCA 
     1381 AGGGGGGTGC CTGTCCCAGT CGGGCCAAAA TGTCAATGAC AGGGGCAGGA AAGTCGCCCC 
     1441 CCTCGGTCCA GAGTTTGGCC ATGAGGCTGT TGAGCATGCC CGGGGCCCAG GGAGCTGCAA 
     1501 CTGCTGGGCC TGAACCCTCT CCGGCAACAA CTGCCGCCCA GGAGGGGCAG CCCAAAGTGC 
     1561 ACCGAGCCCG GAAAACCATG TCCAAACCTA GCAACGGACA GCCTCCAATC CCTGAGAAGC 
     1621 GGCCCCCTGA AGTCCAGCAT TTCCGCATGA GTGATGACAT GCATCTGGGG AAGGTGACTT 
     1681 CAGATGTGGC CAAAAGGAGG AAGCTGAACT CTGGTAGCCT GTCCGAGGAC TTGGGCTCTG 
     1741 CCGGGGGCTC AGGAGATATA ATCCTGGAGA AGGGAGAGCC CAGGCCCCTG GAGGAGTGGG 
     1801 AGACGGTGGT GGGCGATGAC TTCAGCCTGT ACTATGATGC GTACTCTGTG GATGAGCGGG 
     1861 TGGACTCTGA CAGCAAGTCT GAAGTCGAAG CTCTAGCTGA ACAGTTGAGT GAGGAGGAGG 
     1921 AGGAGGAAGA GGAGGAAGAA GAAGAAGAGG AGGAGGAGGA GGAAGAGGAG GAGGAGGAAG 
     1981 AAGAGGACGA GGAGTCGGGC AATCAGTCAG ACAGGAGCGG TTCTAGTGGC CGGCGCAAGG 
     2041 CCAAGAAGAA ATGGCGGAAA GACAGCCCGT GGGTGAAGCC ATCTAGAAAA CGGCGGAAAC 
     2101 GAGAGCCTCC GAGGGCCAAG GAGCCAAGAG GGGTCTCCAA TGACACGTCT TCACTGGAGA 
     2161 CAGAACGCGG GTTTGAGGAG CTGCCCCTCT GCAGCTGCCG CATGGAGGCT CCCAAGATTG 
     2221 ACCGCATCAG CGAGAGAGCA GGGCACAAGT GCATGGCCAC AGAGAGTGTG GATGGAGAGC 
     2281 TCCTGGGCTG CAATGCTGCC ATCCTTAAGC GGGAGACCAT GCGGCCGTCT AGCCGCGTGG 
     2341 CGCTGATGGT GCTCTGTGAG GCCCATCGAG CCCGCATGGT CAAGCACCAT TGCTGCCCGG 
     2401 GCTGCGGCTA CTTCTGCACA GCGGGCACCT TCCTGGAATG CCACCCCGAC TTTCGTGTAG 
     2461 CTCACCGCTT CCATAAGGCC TGCGTATCCC AGCTCAATGG GATGGTCTTC TGTCCCCACT 
     2521 GTGGAGAGGA TGCCTCAGAG GCCCAGGAGG TGACCATTCC TCGGGGCGAT GGGGGAACAC 
     2581 CCCCAATTGG CACCGCAGCT CCTGCTCTGC CACCCCTGGC ACATGATGCC CCAGGGCGAG 
     2641 CGGATACCTC CCAGCCTAGC GCCCGAATGC GAGGGCATGG AGAGCCGCGG CGCCCGCCCT 
     2701 GTGATCCCCT GGCTGACACC ATCGACAGCT CAGGGCCTTC ACTGACTCTG CCTAATGGGG 
     2761 GCTGCCTCTC CGCTGTGGGT CTGCCCCCAG GGCCGGGCAG GGAAGCCCTG GAAAAAGCCT 
     2821 TGGTCATCCA GGAGTCTGAG AGGCGGAAGA AGCTGCGATT CCACCCACGG CAGCTGTACC 
     2881 TGTCGGTGAA GCAGGGGGAG CTGCAGAAGG TGATCCTTAT GCTGTTAGAC AACCTGGACC 
     2941 CCAACTTCCA GAGCGACCAG CAGAGCAAGC GCACGCCCCT GCACGCGGCC GCCCAGAAGG 
     3001 GGTCGGTAGA GATCTGTCAT GTGCTGCTGC AGGCAGGAGC CAACATCAAT GCCGTAGATA 
     3061 AGCAACAACG CACGCCACTA ATGGAGGCCG TGGTGAACAA CCACCTGGAG GTGGCACGCT 
     3121 ACATGGTGCA GTTAGGTGGC TGTGTCTACA GCAAGGAAGA GGATGGCTCC ACCTGTCTAC 
     3181 ATCATGCAGC CAAAATTGGG AACTTGGAAA TGGTCAGCCT GCTACTGAGC ACAGGACAGG 
     3241 TGGACGTCAA TGCCCAGGAC AGTGGGGGCT GGACGCCCAT CATCTGGGCA GCCGAGCACA 
     3301 AGCACATCGA TGTGATTCGT ATGCTGCTGA CCCGGGGTGC CGATGTCACC CTGACTGACA 
     3361 ATGAGGAAAA CATCTGCCTG CACTGGGCCT CCTTCACGGG TAGTGCCGCC ATCGCTGAGG 
     3421 TCCTTCTGAA TGCCCAGTGT GATCTCCATG CTGTCAACTA CCATGGGGAC ACGCCCCTGC 
     3481 ACATAGCCGC CAGGGAGAGC TACCATGACT GTGTTCTGTT GTTCCTGTCT CGTGGAGCCA 
     3541 ACCCTGAGCT TCGGAACAAA GAAGGAGACA CGGCATGGGA TCTGACCCCA GAGCGCTCTG 
     3601 ATGTGTGGTT TGCACTGCAG CTCAATCGAA AGCTTAGGCT TGGGGTAGGG AACCGGGCTG 
     3661 TCCGCACCGA GAAGATCATC TGCCGGGACG TAGCCCGAGG CTATGAGAAT GTACCCATCC 
     3721 CCTGTGTCAA TGGTGTGGAT GGGGAGCCGT GCCCGGAGGA CTACAAGTAC ATCTCTGAGA 
     3781 ACTGCGAGAC ATCGACCATG AACATCGACC GCAACATCAC CCATCTGCAG CACTGCACGT 
     3841 GTGTGGATGA CTGCTCCAGC TCCAATTGCC TATGTGGTCA GCTCAGTATC CGATGCTGGT 
     3901 ATGACAAGGA CGGGCGGCTG CTCCAGGAGT TTAACAAGAT CGAGCCCCCC CTGATCTTTG 
     3961 AGTGTAACCA GGCATGCTCC TGCTGGAGAA GCTGCAAGAA CCGCGTGGTG CAGAGCGGCA 
     4021 TCAAGGTACG GCTGCAGCTC TACCGGACTG CCAAGATGGG CTGGGGGGTC CGAGCCTTGC 
     4081 AGACCATCCC CCAGGGCACG TTCATCTGCG AGTATGTAGG AGAGCTGATC TCTGATGCCG 
     4141 AGGCTGATGT GAGAGAGGAT GATTCTTACC TCTTCGATTT AGATAACAAG GATGGCGAGG 
     4201 TTTACTGCAT TGATGCCCGT TACTATGGCA ACATCAGCCG ATTCATTAAC CACCTGTGTG 
     4261 ACCCCAACAT CATCCCTGTC CGGGTTTTCA TGCTGCACCA AGATCTACGG TTCCCACGCA 
     4321 TTGCCTTCTT CAGCTCCAGG GACATCCGGA CTGGGGAGGA GCTGGGCTTT GACTACGGTG 
     4381 ACCGATTCTG GGACATCAAG AGCAAGTATT TCACCTGCCA GTGTGGCTCT GAGAAGTGCA 
     4441 AGCATTCAGC GGAGGCCATC GCCCTGGAGC AGAGCCGCCT GGCCCGGCTG GACCCCCACC 
     4501 CGGAGCTGCT CCCTGACCTC AGCTCCCTGC CCCCCATCAA CACCACGCGT ACGCGGCCGC 
     4561 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4621 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5341 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5401 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5461 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5521 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5581 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5641 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5701 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5761 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5821 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5881 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5941 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6001 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6061 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6121 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6181 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6241 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6301 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6361 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6421 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6481 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6541 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6601 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6661 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6721 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6781 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6841 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6901 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6961 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7021 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7081 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7141 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7201 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7261 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7321 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7381 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7441 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7501 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7561 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7621 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7801 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7861 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7921 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7981 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8041 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8101 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8161 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8221 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8281 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8341 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//