LOCUS       MR222620                6807 bp    DNA     circular  SYN 
DEFINITION  Hspa1b (Myc-DDK-tagged) - Mouse heat shock protein 1B (Hspa1b)
ACCESSION   NM_010478
VERSION     NM_010478.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2964..3055 
                     /label="Myc-DDK"  
     misc_feature    3085..3670 
                     /label="PolyA signal"  
     misc_feature    3142..3161 
                     /label="Restriction Enzyme"  
     misc_feature    3946..4618 
                     /label="ColE1"  
     misc_feature    5048..5842 
                     /label="Kan/Neo"  
     misc_feature    5877..6179 
                     /label="SV40 ori"  
     misc_feature    6265..6742 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2946..2952 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2946 
                     /label="Hspa1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAGAAC ACGGCGATCG GCATCGACCT GGGCACCACC TACTCGTGCG 
     1081 TGGGCGTGTT CCAGCACGGC AAGGTGGAGA TCATCGCCAA CGACCAGGGC AACCGCACGA 
     1141 CCCCCAGCTA CGTGGCCTTC ACCGACACCG AGCGCCTCAT CGGGGACGCC GCCAAGAACC 
     1201 AGGTGGCGCT GAACCCGCAG AACACCGTGT TCGACGCGAA GCGGCTGATC GGCCGCAAGT 
     1261 TCGGCGATGC GGTGGTGCAG TCCGACATGA AGCACTGGCC CTTCCAGGTG GTGAACGACG 
     1321 GCGACAAGCC CAAGGTGCAG GTGAACTACA AGGGCGAGAG CCGGTCGTTC TTCCCGGAGG 
     1381 AGATCTCGTC CATGGTGCTG ACGAAGATGA AGGAGATCGC TGAGGCGTAC CTGGGCCACC 
     1441 CGGTGACCAA CGCGGTGATC ACGGTGCCCG CCTACTTCAA CGACTCTCAG CGGCAGGCCA 
     1501 CCAAGGACGC GGGCGTGATC GCCGGTCTAA ACGTGCTGCG GATCATCAAC GAGCCCACGG 
     1561 CGGCCGCCAT CGCCTACGGG CTGGACCGGA CCGGCAAGGG CGAGCGCAAC GTGCTCATCT 
     1621 TCGACCTGGG GGGCGGCACG TTCGACGTGT CCATCCTGAC GATCGACGAC GGCATCTTCG 
     1681 AGGTGAAGGC CACGGCGGGC GACACGCACC TGGGAGGGGA GGACTTCGAC AACCGGCTGG 
     1741 TGAGCCACTT CGTGGAGGAG TTCAAGAGGA AGCACAAGAA GGACATCAGC CAGAACAAGC 
     1801 GCGCGGTGCG GCGGCTGCGC ACGGCGTGTG AGAGGGCCAA GAGGACGCTG TCGTCCAGCA 
     1861 CCCAGGCCAG CCTGGAGATC GACTCTCTGT TCGAGGGCAT CGACTTCTAC ACATCCATCA 
     1921 CGCGGGCGCG GTTCGAAGAG CTGTGCTCGG ACCTGTTCCG CGGCACGCTG GAGCCCGTGG 
     1981 AGAAGGCCCT GCGCGACGCC AAGATGGACA AGGCGCAGAT CCACGACCTG GTGCTGGTGG 
     2041 GCGGCTCGAC GCGCATCCCC AAGGTGCAGA AGCTGCTGCA GGACTTCTTC AACGGGCGCG 
     2101 ACCTGAACAA GAGCATCAAC CCGGACGAGG CGGTGGCCTA CGGGGCGGCG GTGCAGGCGG 
     2161 CCATCCTGAT GGGGGACAAG TCGGAGAACG TGCAGGACCT GCTGCTGCTG GACGTGGCGC 
     2221 CGCTGTCGCT GGGCCTGGAG ACTGCGGGCG GCGTGATGAC GGCGCTCATC AAGCGCAACT 
     2281 CCACCATCCC CACCAAGCAG ACGCAGACCT TCACCACCTA CTCGGACAAC CAGCCCGGGG 
     2341 TGCTGATCCA GGTGTACGAG GGCGAGAGGG CCATGACGCG CGACAACAAC CTGCTGGGGC 
     2401 GCTTCGAGCT GAGCGGCATC CCGCCGGCGC CCAGGGGCGT GCCGCAGATC GAGGTGACCT 
     2461 TCGACATCGA CGCCAACGGC ATCCTGAACG TCACGGCCAC CGACAAGAGC ACCGGCAAGG 
     2521 CCAACAAGAT CACCATCACC AACGACAAGG GCCGCCTGAG CAAGGAGGAG ATCGAGCGCA 
     2581 TGGTGCAGGA GGCCGAGCGC TACAAGGCCG AGGACGAGGT GCAGCGCGAC AGGGTGGCCG 
     2641 CCAAGAACGC GCTCGAGTCC TATGCCTTCA ACATGAAGAG CGCCGTGGAG GACGAGGGTC 
     2701 TCAAGGGCAA GCTCAGCGAG GCTGACAAGA AGAAGGTGCT GGACAAGTGC CAGGAGGTCA 
     2761 TCTCCTGGCT GGACTCCAAC ACGCTGGCCG ACAAGGAGGA GTTCGTGCAC AAGCGGGAGG 
     2821 AGCTGGAGCG GGTGTGCAGC CCCATCATCA GTGGGCTGTA CCAGGGTGCG GGTGCTCCTG 
     2881 GGGCTGGGGG CTTCGGGGCC CAGGCGCCGC CGAAAGGAGC CTCTGGCTCA GGACCCACCA 
     2941 TCGAGGAGGT GGATACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3001 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3061 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3121 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3181 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3241 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3301 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3361 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3421 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3481 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3541 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3601 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3661 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3721 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3781 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     3841 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     3901 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     3961 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4021 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4081 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4141 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4201 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4261 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4321 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4381 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4441 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4501 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4561 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4621 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4681 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4741 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4801 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     4861 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     4921 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     4981 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5041 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5101 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5161 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5221 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5281 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5341 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5401 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5461 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5521 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5581 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5641 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5701 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5761 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     5821 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     5881 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     5941 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6001 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6061 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6121 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6181 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6241 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6301 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6361 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6421 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6481 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6541 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6601 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6661 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6721 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6781 ATTTAACAAA AATTTAACGC GAATTTT
//