LOCUS       MR222600                8805 bp    DNA     circular  SYN 
DEFINITION  Setdb1 (Myc-DDK-tagged) - Mouse SET domain, bifurcated 1 (Setdb1), transcript variant 2
ACCESSION   NM_018877
VERSION     NM_018877.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4962..5053 
                     /label="Myc-DDK"  
     misc_feature    5083..5668 
                     /label="PolyA signal"  
     misc_feature    5140..5159 
                     /label="Restriction Enzyme"  
     misc_feature    5944..6616 
                     /label="ColE1"  
     misc_feature    7046..7840 
                     /label="Kan/Neo"  
     misc_feature    7875..8177 
                     /label="SV40 ori"  
     misc_feature    8263..8740 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4944..4950 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4944 
                     /label="Setdb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTCCCTC CCTGGGTGCA TGAGTTTGGC TGCAGCGCCA GCTGCAGCTG 
     1081 ACTCTGCAGA GATTGCTGAG CTGCAGCAGG CGGTGGTTGA AGAGCTGGGT ATCTCTATGG 
     1141 AGGAACTTCG TCAGTACATT GATGAGGAAC TGGAAAAGAT GGACTGCATA CAGCAGCGCA 
     1201 AGAAGCAGCT CGCAGAGCTG GAGACGTGGG TACTACAGAA AGAGTCTGAA GTGGCTTATG 
     1261 TTGATCGGCT GTTTGATGAT GCATCCAGGG AAGTGACTAA CTGTGAGTCT TTGGTGAAGG 
     1321 ATTTCTACTC TAAGCTGGGA CTACAGTATC ATGACAGTAG CTCTGAGGAT GAAGCTTCCC 
     1381 GGCCCACAGA GATCATTGAG ATTCCTGATG AAGATGATGA TGTCCTCAGT ATTGATTCAG 
     1441 GTGATGCTGG GAGCAGAACT CCAAAAGACC AGAAGCTTCG TGAAGCTATG GCTGCCTTAA 
     1501 GAAAATCAGC TCAAGATGTC CAGAAGTTCA TGGATGCTGT CAACAAGAAA AGCAGTTCTC 
     1561 AAGATCTACA TAAAGGAACC TTGGGTCAGG TGTCTGGAGA ACTGAGCAAA GATGGGGACC 
     1621 TGATAGTCAG CATGCGGATT CTGGGCAAGA AGAGGACTAA GACATGGCAC AAAGGCACCC 
     1681 TTATTGCCAT CCAGACTGTT GGGCTAGGAA AAAAATACAA AGTGAAATTT GACAACAAAG 
     1741 GAAAGAGTCT GCTATCTGGG AACCATATTG CCTATGATTA CCACCCTCCC GCTGACAAGC 
     1801 TGTTTGTGGG CAGTCGAGTG GTGGCCAAGT ACAAAGATGG AAATCAGGTC TGGCTTTATG 
     1861 CTGGCATTGT AGCTGAGACC CCTAACGTCA AGAACAAGCT CAGATTTTTA ATTTTTTTTG 
     1921 ATGATGGCTA TGCTTCCTAT GTCACTCAGT CAGAGCTTTA TCCCATTTGC CGACCACTAA 
     1981 AAAAGACTTG GGAGGACATA GAAGATAGCT CCTGCCGAGA CTTCATAGAG GAATATATCA 
     2041 CTGCCTATCC AAACCGCCCA ATGGTACTTC TCAAGAGTGG GCAGCTTATC AAGACTGAGT 
     2101 GGGAAGGCAC ATGGTGGAAG TCTCGAGTTG AAGAGGTGGA TGGCAGCCTA GTCAGGATCC 
     2161 TCTTTCTGGA TGACAAAAGA TGTGAGTGGA TATATCGAGG CTCTACACGC CTGGAACCTA 
     2221 TGTTTAGTAT GAAGACATCC TCAGCCTCTG CAATGGAGAA GAAGCAAGGG GGGCAACTCA 
     2281 GAACCCGTCC TAATATGGGT GCTGTGAGGA GCAAAGGTCC TGTTGTTCAG TATACACAGG 
     2341 ATCTAACTGG TACTGGAATC CAGTTTAAGC CCATGGAGCC CCTACAGCCT ATAGCTCCAC 
     2401 CGGCCCCACT TCCTATACCT CCTCTTTCCC CCCAAGCAGC TGACACTGAA AGCTTAGAAA 
     2461 GCCAACTTGC ACAATCACGG AAACAAGTAG CCAAGAAGAG CACATCATTC CGACCAGGAT 
     2521 CTGTGGGCTC CGGCCATTCC TCCCCTACTT CATCCACACT CAGTGAAAAT GTGTCTGCTG 
     2581 GGAAACTTGG GATAAACCAG ACATATCGGT CACCTTTGGC CTCAGTAACA TCTACCCCAG 
     2641 CATCTGCAGC CCCTCCAGTC CCTCCAGTCC CACCAGGGCC TCCAACCCCT CCAGGGCCTC 
     2701 CAGCTCCTCC AGGGCCTCTA GCTCCTCCAG CCTTCCATGG CATGTTAGAG CGGGCACCAG 
     2761 CTGAGCCCTC CTACCGAGCC CCCATGGAGA AGCTTTTCTA TTTACCTCAT GTCTGCAGTT 
     2821 ACACTTGTTT GTCCCGGATC AGACCCATGA GAAACGAACA GTATCGGGGC AAGAACCCTC 
     2881 TATTAGTTCC ACTTCTGTAT GACTTCCGGA GGATGACAGC ACGGCGCAGA GTTAACCGCA 
     2941 AAATGGGCTT TCATGTAATC TATAAGACAC CCTGTGGTCT CTGCCTTCGG ACGATGCAGG 
     3001 AGATAGAGCG CTACCTTTTT GAGACTGGCT GTGACTTTCT GTTCCTGGAG ATGTTCTGTT 
     3061 TGGATCCATA TGTTCTTGTT GACAGAAAGT TTCAACCCTT TAAGCCTTTT TACTATATTT 
     3121 TGGACATCAC CTATGGCAAG GAAGATGTTC CCCTGTCCTG TGTTAATGAG ATTGACACAA 
     3181 CTCCCCCACC CCAGGTGGCC TACAGCAAGG AACGCATTCC TGGCAAGGGT GTTTTCATTA 
     3241 ACACAGGCCC TGAATTTCTG GTTGGCTGTG ACTGCAAGGA TGGGTGTCGG GATAAATCCA 
     3301 AATGTGCCTG CCACCAGCTA ACTATCCAGG CCACAGCCTG TACCCCAGGG GGCCAAGTCA 
     3361 ACCCTAACTC TGGCTACCAG TATAAAAGAC TAGAAGAGTG TCTGCCCACA GGGGTTTATG 
     3421 AGTGTAACAA ACGCTGCAAT TGTGACCCAA ACATGTGCAC AAATCGGTTG GTGCAGCATG 
     3481 GTCTGCAGGT TCGACTACAG CTGTTTAAGA CACAGAACAA GGGCTGGGGT ATCCGCTGCT 
     3541 TGGATGATAT TGCCAAAGGC TCTTTTGTCT GCATTTATGC AGGCAAAATC CTGACAGATG 
     3601 ACTTTGCAGA CAAAGAAGGC CTGGAGATGG GTGATGAGTA CTTTGCAAAT CTGGACCACA 
     3661 TTGAAAGTGT GGAGAACTTC AAGGAAGGAT ATGAGAGTGA TGTCCCCACT TCCTCTGACA 
     3721 GCAGTGGGGT AGATATGAAG GACCAGGAAG ATGGCAACAG CGGTTCAGAG GACCCTGAAG 
     3781 AATCCAATGA TGACAGCTCT GATGATAACT TCTGTAAGGA TGAGGACTTC AGCACCAGTT 
     3841 CAGTGTGGCG TAGCTATGCT ACCCGGAGGC AGACTCGGGG TCAAAAGGAG AATGAATTGT 
     3901 CTGAGATGAC TTCCAAGGAC TCCCGCCCCC CAGACCTCGG GCCTCCACAT GTTCCTATCC 
     3961 CTTCCTCAGT ATCTGTAGGG GGCTGCAATC CACCTTCCTC TGAAGAGACA CCCAAGAACA 
     4021 AGGTGGCCTC GTGGTTGAGT TGCAATAGTG TCAGTGAAGG TGGATTTGCT GACTCTGACA 
     4081 GCCGTTCTTC CTTCAAGACT AGTGAAGGTG GAGATGGCCG TGCTGGGGGA GGCCGGGGAG 
     4141 AGGCTGAAAG GGCCTCTACC TCAGGATTGA GCTTCAAGGA TGAAGGAGAC AATAAGCAGC 
     4201 CTAAAAAAGA GGACCCTGAG AACCGAAACA AGATGCCAGT AGTTACTGAA GGCTCTCAGA 
     4261 ATCATGGACA TAATCCTCCC ATGAAGTCTG AAGGGCTTCG CCGATCAGCT AGTAAAATGT 
     4321 CTGTGCTCCA GAGCCAGCGA GTTGTGACTT CTACTCAGTC AAACCCTGAT GACATCCTGA 
     4381 CACTGTCCAG CAGCACAGAG AGTGAGGGGG AAAGTGGAAC CAGCCGAAAG CCCACTGCTG 
     4441 GTCACACTTC AGCCACAGCT GTTGATAGTG ATGACATCCA GACCATCTCT TCTGGCTCTG 
     4501 ACGGTGATGA CTTTGAGGAC AAGAAGAACT TGTCAGGACC AACAAAGCGC CAGGTGGCAG 
     4561 TAAAATCAAC CCGAGGCTTT GCTCTTAAAT CAACCCATGG TATTGCCATT AAATCAACCA 
     4621 ACATGGCTTC CGTGGACAAG GGGGAGAGTG CACCAGTTCG TAAGAACACA CGCCAGTTCT 
     4681 ATGATGGTGA AGAGTCTTGC TACATCATTG ATGCCAAACT TGAAGGCAAC CTAGGCCGCT 
     4741 ACCTCAATCA CAGTTGCAGC CCCAACCTGT TTGTCCAGAA TGTGTTTGTG GATACCCATG 
     4801 ATCTTCGCTT CCCTTGGGTG GCCTTCTTTG CCAGCAAGAG AATCCGGGCT GGAACAGAAC 
     4861 TCACTTGGGA CTACAACTAC GAAGTGGGCA GTGTGGAAGG CAAGGAGCTG CTGTGCTGCT 
     4921 GTGGGGCCAT TGAATGCAGA GGGAGACTTC TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4981 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5041 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5101 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5161 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5221 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5281 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5341 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5401 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5461 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5521 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5581 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5641 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5701 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5761 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5821 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5881 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5941 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6001 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6061 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6121 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6181 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6241 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6301 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6361 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6421 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6481 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6541 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6601 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6661 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6721 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6781 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6841 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6901 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6961 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7021 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7081 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7141 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7201 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7261 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7321 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7381 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7441 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7501 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7561 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7621 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7681 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7741 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7801 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7861 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7921 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7981 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8041 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8101 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8221 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8281 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8341 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8401 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8461 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8521 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8581 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8641 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8701 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8761 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//