LOCUS       MR222596                8700 bp    DNA     circular  SYN 
DEFINITION  Prdm16 (Myc-DDK-tagged) - Mouse PR domain containing 16 (Prdm16), transcript variant 1
ACCESSION   NM_027504
VERSION     NM_027504.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4855..4946 
                     /label="Myc-DDK"  
     misc_feature    4976..5561 
                     /label="PolyA signal"  
     misc_feature    5033..5052 
                     /label="Restriction Enzyme"  
     misc_feature    5837..6509 
                     /label="ColE1"  
     misc_feature    6939..7733 
                     /label="Kan/Neo"  
     misc_feature    7768..8070 
                     /label="SV40 ori"  
     misc_feature    8156..8633 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4845..4853 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4845 
                     /label="Prdm16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGATCCAAG GCGAGGGCGA GGAAGCTAGC CAAAAGTGAC GGTGACGTTG 
     1081 TAAATAATAT GTATGAACCT GACCCGGACC TGCTGGCCGG CCAGAGTGCC GAGGAGGAGA 
     1141 CCGAAGACGG CATCCTGTCC CCCATCCCCA TGGGGCCACC GTCCCCCTTC CCCACCAGCG 
     1201 AGGACTTCAC TCCCAAGGAG GGCTCGCCCT ATGAGGCTCC TGTCTACATT CCTGAAGACA 
     1261 TTCCAATCCC ACCAGACTTC GAGCTACGAG AGTCCTCCAT ACCAGGAGCT GGCCTGGGGA 
     1321 TCTGGGCCAA GCGGAAGATG GAAATCGGGG AGAGGTTTGG CCCCTACGTG GTGACGCCCC 
     1381 GGGCCGCACT GAAGGAGGCC GACTTTGGAT GGGAGATGCT GACGGATACA GAGGTGTCAT 
     1441 CCCAGGAGAG CTGCATCAAA AAGCAGATCT CTGAAGACTT GGGTAGCGAG AAGTTCTGCG 
     1501 TGGATGCCAA TCAGGCGGGG TCTGGCAGCT GGCTCAAGTA CATCCGTGTA GCGTGTTCCT 
     1561 GTGATGACCA AAACCTCGCC ATGTGTCAGA TCAACGAACA GATTTACTAT AAAGTCATTA 
     1621 AGGACATCGA GCCTGGAGAG GAACTGTTGG TGCATGTGAA AGAAGGTGCC TACTCCTTGG 
     1681 GTGTCATGGC CCCCAGCTTG GATGAGGACC CCACATTCCG CTGTGATGAG TGTGATGAGC 
     1741 TCTTCCAGTG CAGGCTGGAC CTGAGGCGCC ACAAGAAGTA CGCGTGCAGC TCTGCAGGAG 
     1801 CCCAGCTCTA CGAGGGCCTA GGGGAGGAAC TCAAGCCCGA GGGCCTTGGC GTGGGCAGCG 
     1861 ACGGGCAAGC GCATGAGTGC AAGGATTGCG AGCGGATGTT CCCCAACAAG TACAGCTTGG 
     1921 AGCAACACAT GATCGTCCAC ACGGAAGAGC GTGAGTACAA ATGTGACCAG TGTCCCAAGG 
     1981 CCTTCAACTG GAAGTCCAAC CTCATCCGCC ACCAGATGTC TCACGACAGT GGCAAGCGCT 
     2041 TCGAATGTGA AAACTGTGTC AAGGTGTTCA CGGACCCCAG CAACCTCCAG CGTCACATCC 
     2101 GCTCACAGCA TGTCGGTGCC CGGGCCCATG CCTGCCCTGA CTGTGGCAAG ACCTTCGCCA 
     2161 CATCCTCTGG CCTCAAACAG CACAAGCATA TCCACAGCAC GGTGAAGCCA TTCATATGCG 
     2221 AGGTCTGCCA CAAGTCCTAC ACGCAGTTCT CCAACCTGTG CCGGCACAAG CGGATGCACG 
     2281 CCGACTGCAG GACGCAGATC AAGTGCAAGG ACTGTGGGCA GATGTTCAGC ACTACCTCCT 
     2341 CCCTCAACAA GCATCGGAGA TTCTGCGAGG GCAAGAACCA TTACACGCCT GGCAGCATCT 
     2401 TCACCCCAGG CCTGCCCTTG ACCCCCAGCC CCATGATGGA CAAGACAAAA CCCTCCCCGA 
     2461 CCCTCAACCA CGGGGGCCTA GGCTTCAGCG AGTACTTCCC CTCCAGACCT CATCCTGGGA 
     2521 GCCTGCCCTT CTCGGCTGCT CCTCCGGCCT TCCCCGCACT CACTCCGGGC TTCCCGGGCA 
     2581 TCTTTCCTCC ATCCCTGTAC CCACGACCAC CTCTGCTACC TCCCACGCCG CTGCTCAAGA 
     2641 GCCCCCTGAA CCACGCGCAG GACGCCAAGC TACCCAGCCC GCTGGGAAAC CCAGCCCTGC 
     2701 CCCTTGTCTC CGCGGTCAGC AATAGCAGCC AGGGTGCCAC AGCGGCCACC GGGTCAGAGG 
     2761 AGAAATTTGA TGGCCGCTTG GAAGACGCAT ATGCGGAGAA GGTCAAAAAT AGGAGCCCTG 
     2821 ACATGTCGGA TGGCAGTGAC TTTGAGGATA TCAACACCAC GACCGGGACA GACTTGGACA 
     2881 CTACCACGGG CACGGGGTCA GACCTGGACA GCGACCTGGA CAGTGACAGA GACAAAGGCA 
     2941 AGGACAAGGG GAAGCCAGTG GAGAGCAAAC CTGAGTTTGG GGGTGCATCT GTGCCCCCTG 
     3001 GGGCCATGAA CAGTGTGGCC GAGGTACCGG CCTTCTACTC ACAGCATTCC TTCTTCCCGC 
     3061 CACCCGAGGA ACAGCTGCTG ACGGCCTCGG GAGCTGCCGG CGACTCCATC AAGGCCATCG 
     3121 CGTCCATCGC GGAGAAATAC TTCGGTCCTG GCTTCATGAG CATGCAGGAG AAGAAGCTGG 
     3181 GCTCACTACC CTACCACTCC GTGTTCCCCT TCCAGTTCCT GCCTAACTTT CCCCACTCCC 
     3241 TCTACCCCTT TACGGACCGA GCCCTCGCCC ACAACTTGCT GGTCAAGGCT GAGCCAAAGT 
     3301 CACCCCGGGA TGCCCTCAAG GTGGGCGGCC CCAGTGCGGA GTGCCCCTTC GACCTCACCA 
     3361 CCAAACCAAA AGAGGCCAAA CCCGCCCTGC TCGCACCCAA GGTCCCCCTC ATCCCCTCAT 
     3421 CTGGCGAGGA ACAGCCACTG GACCTGAGCA TCGGCAGCAG GGCCAGGGCA AGCCAGAACG 
     3481 GAGGTGGCCG TGAGCCGCGG AAGAACCACG TCTACGGTGA ACGGAAGCCG GGGGTCAGCG 
     3541 AGGGGCTGCC TAAGGTGTGC CCAGCACAGC TGCCCCAGCA GCCCTCCTTG CATTATGCTA 
     3601 AGCCTTCACC GTTCTTCATG GATCCCATCT ACAGGGTAGA AAAGCGGAAG GTGGCAGACC 
     3661 CTGTGGGAGT CCTGAAAGAG AAGTACCTGC GGCCGTCCCC ACTTCTGTTC CACCCCCAGA 
     3721 TGTCAGCCAT AGAAACCATG ACGGAGAAGC TGGAGAGCTT TGCAGCCATG AAGGCCGACT 
     3781 CAGGCAGCTC CCTGCAGCCC CTGCCTCACC ACCCGTTCAA CTTCCGCTCC CCACCCCCAA 
     3841 CGCTCTCGGA TCCCATCCTC AGGAAGGGGA AGGAGAGATA CACGTGCAGG TACTGTGGCA 
     3901 AGATCTTCCC CAGATCTGCA AATCTCACAA GACATCTGAG GACACACACA GGGGAGCAGC 
     3961 CATACAGGTG CAAGTACTGT GACCGGTCAT TCAGCATCTC CTCCAACCTC CAGCGGCACG 
     4021 TGAGGAACAT CCACAACAAA GAGAAGCCGT TCAAGTGCCA TCTGTGCAAC CGCTGCTTCG 
     4081 GGCAGCAGAC CAACCTAGAC CGGCACCTGA AGAAGCACGA ACACGAGGGC GCACCAGTGA 
     4141 GCCAGCACTC CGGGGTGCTC ACGAACCACC TGGGCACCAG CGCCTCCTCC CCCACCTCCG 
     4201 AGTCGGACAA CCATGCACTT TTAGATGAGA AGGAAGATTC TTACTTCTCC GAGATCCGAA 
     4261 ACTTCATCGC CAACAGCGAG ATGAACCAGG CATCCACTCG AATGGACAAA CGGCCTGAGA 
     4321 TCCAAGACCT GGACAGCAAC CCACCGTGTC CAGGCTCAGC CAGTGCAAAG CCAGAGGACG 
     4381 TAGAGGAGGA GGAAGAGGAG GAGCTGGAGG AAGAGGATGA TGACAGCTTA GCCGGGAAGT 
     4441 CACAGGAGGA CACGGTGTCC CCCACACCTG AGCCCCAAGG AGTCTATGAA GATGAAGAGG 
     4501 ATGAGGAACC ACCCAGCCTG ACCATGGGCT TTGACCATAC CCGGAGGTGT GTTGAGGAGC 
     4561 GAGGAGGCGG CCTGTTAGCT TTGGAGCCGA CGCCGACCTT TGGGAAGGGG CTGGATCTCC 
     4621 GCAGAGCAGC TGAGGAAGCA TTTGAAGTTA AAGATGTGCT TAATTCCACC TTAGATTCTG 
     4681 AGGTTTTAAA ACAAACCCTG TACAGGCAGG CTAAGAACCA GGCATATGCA ATGATGCTGT 
     4741 CCCTCTCTGA AGACACTCCT CTCCACGCCC CCTCCCAGAG CTCACTGGAT GCTTGGTTGA 
     4801 ACATCACAGG ACCCTCGTCA GAGTCCGGAG CCTTTAACCC CATCAACCAC CTCACGCGGC 
     4861 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4921 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4981 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5041 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5101 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5161 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5221 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5281 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5341 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5401 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5461 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5521 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5581 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5641 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5701 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5761 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5821 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5881 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5941 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6001 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6061 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6121 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6181 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6241 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6301 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6361 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6421 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6481 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6541 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6601 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6661 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6721 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6781 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6841 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6901 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6961 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7021 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7081 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7141 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7201 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7261 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7321 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7381 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7441 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7501 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7561 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7621 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7681 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7741 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7801 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7861 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7921 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7981 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8041 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8101 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8161 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8221 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8281 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8341 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8401 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8461 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8521 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8581 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8641 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//