LOCUS       MR222584                8361 bp    DNA     circular  SYN 
DEFINITION  Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (Tsc1)
ACCESSION   NM_022887
VERSION     NM_022887.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4518..4609 
                     /label="Myc-DDK"  
     misc_feature    4639..5224 
                     /label="PolyA signal"  
     misc_feature    4696..4715 
                     /label="Restriction Enzyme"  
     misc_feature    5500..6172 
                     /label="ColE1"  
     misc_feature    6602..7396 
                     /label="Kan/Neo"  
     misc_feature    7431..7733 
                     /label="SV40 ori"  
     misc_feature    7819..8296 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4500..4506 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4500 
                     /label="Tsc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGTTA GCCAACATTG GGGAGCTGCT CTCCATGCTG GACTCCTCCA 
     1081 CACTGGGTGT GCGGGATGAC GTGACAGCCA TCTTCAAGGA GTCCCTCAAT TCTGAACGTG 
     1141 GGCCTATGCT TGTCAACACG TTGGTTGATT ATTACCTGGA AACCAATTCT CAGCCGGTAT 
     1201 TGCACATCCT GACCACCTTG CAGGAGCCAC ACGATAAGCA CCTCTTGGAC AAAATTAATG 
     1261 AGTATGTAGG CAAAGCTGCT ACCCGTTTAT CCATCCTCTC GCTGCTGGGG CATGTTGTGA 
     1321 GACTGCAGCC ATCTTGGAAG CATAAGCTCT CTCAAGCACC TCTTCTGCCT TCTTTATTGA 
     1381 AATGTCTCAA GATGGACACT GATGTTGTGG TCCTCACAAC TGGTGTCTTG GTGTTGATCA 
     1441 CCATGCTCCC GATGATCCCG CAGTCAGGGA AGCAGCACCT TCTCGACTTC TTTGACATCT 
     1501 TTGGCCGTCT CTCGTCATGG TGCCTGAAGA AACCAGGCCA TGTGACAGAA GTGTACCTGG 
     1561 TCCATCTTCA TGCCAGTGTT TATGCCCTCT TTCACCGCCT TTATGGGATG TACCCATGTA 
     1621 ACTTCGTCTC CTTCCTGCGC TCTCACTACA GTATGAAGGA AAACGTGGAG ACTTTTGAAG 
     1681 AAGTGGTCAA GCCAATGATG GAGCATGTGC GAATTCACCC GGAATTAGTG ACTGGATCCA 
     1741 AGGACCATGA ACTGGACCCT CGAAGGTGGA AGACATTAGA AACTCATGAT GTTGTAATAG 
     1801 AGTGTGCCAA AATCTCTCTG GACCCTACAG AAGCCTCGTA TGAAGATGGC TATTCTGTGT 
     1861 CACACCAGCT CTCTGCTTGC TTCCCTTACC GTTCAGCTGA TGTCACCACC AGCCCTTATG 
     1921 TGGACACACA GAATAGCTAT GGGGGTTCTA CTTCCACCCC TTCCTCCAGC TCTCGGCTGA 
     1981 TGTTGTTCAG TCCACCTGGG CAGCTACCTC AGAGTTTGAG TTCCCCATCA ACACGGCTGT 
     2041 TACCTGAGCC GCTGCAAGCT AGTCTCTGGA GCCCATCTGC GGTCTGTGGT ATGACCACTC 
     2101 CTCCTACGTC TCCTGGAAAT GTCCCAGCTG ATTTGTCACA TCCGTATAGT AAAGCCTTTG 
     2161 GTACCACTGG TGGAAAAGGA ACTCCTTCAG GAACCCCAGC GACCTCTCCT CCTCCAGCCC 
     2221 CACCTTGTCC CCAAGATGAC TGTGTGCATG GTTCAGCAGC CCAGGCCTCA GCCACAGCCC 
     2281 CCAGGAAGGA AGAAAGAGCA GATTCCTCAA GGCCTTACCT TCACAGACAG TCAAACGACC 
     2341 GAGGATTAGA GGATCCACCT GGAAGCAAAG GTTCCGTTAC TCTGAGGAAT CTACCTGATT 
     2401 TCCTAGGTGA TCTGGCTTCT GAGGAAGACA GTATCGAGAA AGATAAGGAA GAAGCTGCAA 
     2461 TATCTAAAGA GCTTTCTGAG ATCACTACTG CAGAGGCGGA TCCTGTAGTT CCTCGAGGGG 
     2521 GCTTTGACTC TCCCTTCTAC CGAGACAGTC TCTCTGGCTC TCAGCGGAAG ACTCATTCGG 
     2581 CAGCCTCTGG GACTCAGGGC TCCAGCGTGA ACCCTGAGCC TTTGCACTCC TCCCTGGACA 
     2641 AACATGGGCC TGACACACCA AAGCAAGCCT TTACTCCCAT AGACCCACCC TCTGGCAGTG 
     2701 CTGATGTCAG TCCCGCTGGG GACAGGGATC GCCAGACTTC TCTGGAGACC AGTATCCTCA 
     2761 CTCCCAGCCC TTGCAAAATC CCACCTCAGA GGGGAGTGAG CTTTGGAAGT GGGCAGCTTC 
     2821 CCCCATATGA TCATCTCTTT GAGGTGGCCT TGCCAAAGAC TGCCTGTCAC TTTGTCAGCA 
     2881 AGAAGACTGA GGAGCTGTTG AAGAAAGTGA AAGGAAACCC TGAGGAAGAC TGTGTGCCCT 
     2941 CTACCTCCCC AATGGAAGTA CTGGACAGAC TGATAGAGCA GGGAGCAGGT GCGCACAGCA 
     3001 AGGAGCTGAG CAGGTTGTCC CTGCCCAGCA AGTCTGTTGA CTGGACCCAC TTTGGAGGCT 
     3061 CTCCTCCCTC AGATGAGCTC CGGACCCTCC GAGACCAGTT GCTTTTACTG CACAATCAGC 
     3121 TGCTGTACGA GCGCTTCAAG CGGCAGCAGC ATGCACTCAG GAACAGAAGG CTCCTGCGCA 
     3181 AGGTGATCCG AGCAGCGGCT CTGGAGGAAC ACAATGCAGC AATGAAAGAT CAGTTGAAGT 
     3241 TACAAGAGAA GGACATACAG ATGTGGAAGG TGAGTCTGCA GAAAGAACAA GCCCGATACA 
     3301 GTCAGCTTCA GGAACAACGT GACACCATGG TGACCCAACT GCATAGCCAG ATCAGACAGC 
     3361 TACAGCATGA CCGAGAAGAA TTCTACAACC AGAGTCAGGA GTTACAGACA AAGCTGGAGG 
     3421 ACTGCAGAAA CATGATTGCG GAGCTTCGGG TGGAGCTGAA GAAGGCTAAC AACAAGGTGT 
     3481 GCCACACTGA GCTGCTGCTC AGCCAGGTCT CTCAGAAGCT CTCCAACAGT GAGTCAGTGC 
     3541 AGCAGCAGAT GGAGTTCTTG AATAGGCAGC TCCTGGTGCT CGGGGAGGTC AATGAGCTGT 
     3601 ACCTGGAGCA GCTGCAGAGC AAGCATCCTG ACACCACCAA GGAAGTAGAA ATGATGAAAA 
     3661 CTGCATATCG GAAAGAGCTA GAGAAAAACA GAAGCCACCT TCTCCAGCAG AACCAAAGGT 
     3721 TGGACGCCTC ACAGAGGCGA GTTTTGGAAC TGGAGTCTCT TCTGGCCAAG AAAGACCACC 
     3781 TTCTCCTAGA ACAGAAGAAA TATCTTGAGG ATGTCAAGAG CCAGGCGAGT GGACAGCTGC 
     3841 TGGCTGCAGA GAGCAGGTAT GAGGCTCAGA GAAAGATCAC CCGGGTGTTG GAACTGGAGA 
     3901 TCCTAGACTT GTATGGCAGG TTGGAAAAAG ATGGCCGCCT ACGGAAACTA GAAGAGGACA 
     3961 GAGCAGAGGC AGCAGAGGCA GCAGAAGAGA GGCTTGACTG TTGTAGTGAT GGATGCACAG 
     4021 ATTCCTTGGT AGGACATAAT GAAGAGGCTT CTGGTCACAA TGGTGAGACC AGGACCTCCA 
     4081 GACCTGGTGG CACCCGGGCC AGCTGTGGAG GTAGAGTCAC TGGAGGCAGC AGCAGCAGCA 
     4141 GCAGTGAGCT TTCCACTCCA GAGAAACCCC CGAGCCAGAG GTTCAGCAGC CGGTGGGAAC 
     4201 CTGCCCTGGG CGAGCCCTCC AGCAGCATCC CCACCACTGT TGGCTCACTT CCCAGTTCCA 
     4261 AAAGCTTCCT GGGCATGAAG GCCCGGGAGC TGTTCCGTAA TAAGAGCGAG AGCCAGTGTG 
     4321 ATGAGGACAG CGTGACCATG AGTAGCAGCA GCCTTTCTGA GACCCTGAAG ACAGAACTGG 
     4381 GCAAGGACTC GGGCACAGAA AACAAGACTT CCCTGAGTCT AGATGCCCCA CACCCATCTT 
     4441 CCCCAAACTC AGACAATGTG GGGCAGCTCC ACATCATGGA CTACAATGAG ACTCATCCTG 
     4501 AACACAGCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4561 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4621 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4681 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4741 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4801 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4861 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4921 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4981 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5041 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5101 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5161 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5221 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5281 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5341 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5401 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5461 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5521 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5581 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5641 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5701 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5761 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5821 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5881 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5941 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6001 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6061 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6121 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6181 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6241 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6301 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6361 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6421 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6481 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6541 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6601 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6661 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6721 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6781 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6841 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6901 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6961 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7021 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7081 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7141 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7201 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7261 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7321 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7381 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7441 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7501 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7561 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7801 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7861 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7921 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7981 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8041 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8101 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8161 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8221 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8281 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8341 CAAAAATTTA ACGCGAATTT T
//