LOCUS       MR222530                8544 bp    DNA     circular  SYN 
DEFINITION  Prg4 (Myc-DDK-tagged) - Mouse proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein) (Prg4), transcript variant 1. Note: ORF is codon optimized
ACCESSION   NM_021400
VERSION     NM_021400.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4701..4792 
                     /label="Myc-DDK"  
     misc_feature    4822..5407 
                     /label="PolyA signal"  
     misc_feature    4879..4898 
                     /label="Restriction Enzyme"  
     misc_feature    5683..6355 
                     /label="ColE1"  
     misc_feature    6785..7579 
                     /label="Kan/Neo"  
     misc_feature    7614..7916 
                     /label="SV40 ori"  
     misc_feature    8002..8479 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4683..4689 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4683 
                     /label="Prg4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCTGGAAG ATTCTTCCTG TTTGTCTGTC TCTGCTGCTT CCCGTGGTCC 
     1081 TCATTCAGCA AGTCTCATCT CAAGACCTCA GTTCTTGTGC TGGAAGGTGC GGAGAAGGCT 
     1141 ACTCCCGGGA CGCTACCTGC AATTGTGATT ACAACTGTCA ACACTACATG GAGTGTTGCC 
     1201 CAGATTTCAA GAGGGTCTGT ACCACCGAAC TGTCCTGCAA AGGCCGGTGC TTTGAATCTT 
     1261 TTGCACGGGG GCGGGAGTGC GACTGTGATT CTCAGTGTAA ACAGTACGGT AAGTGTTGCG 
     1321 CCGATTACGA TAGTTTCTGT GAAGAGGTTC ACAATCCTAC CAGCCCGAGC CCTAAAACTG 
     1381 CTCCTCCTCC TCCTGGCGCC TCACATACCA TTAAGAGCAC TACAAAACGC AGCCCGAAAA 
     1441 GCCCCAAGAA AAAAACAAAG AAGGTCGTCG AAAGTGAGGA GATCACTGAA GAACACTCCG 
     1501 TCTCAGAGAA TCAGGAGTCC AGCTCCAGCA GCTCTTCCAG TTCAAGCACA ATACGCAAGA 
     1561 TCAAGAGCTC AAAAAACAGT GCCAACAGGG AGCTGCAGAA GAATCCTAAA GTCAAAGATA 
     1621 ATAAGAAAAA CACTCCTAAA AAGAAGCCAA ACCCCGAACC CCCCGCTGTT GACGAGGCTG 
     1681 GCTCAGGCTT GGACAATGGG GAGTTTAAGC TCACACCTCC CCCGCCCGAT CCTCCGACAA 
     1741 CTCCACACTC TAAAGTGACA ACATCTCCTA AGACCACTGC TGCTAAGCCG GTTACCCCCA 
     1801 AGCCCAGCCT CGCACCAAAC TCTGAGACTA GCAAAGAAGC TAGCCTGGCC AGCAACAAAG 
     1861 AAACTACAGT GGAGACTAAA GAAACCACAG CCACAAATAA GCAGTCTAGT GCAAGCAAAA 
     1921 AGAAGACAAC CAGTGCAAAG GAAACGCGAT CCGCAGAGAA GACTTCTGAC AAGGACGTCG 
     1981 AGCCGACCTC CACCACACCC AAGAACTCAG CGCCAACTAC AACCAAAAAG CCCGTAACGA 
     2041 CAACAAAGGA GCCAGTCCCC ACAACCCCCA AAGAACCTGA GCCTACTACA CCCAAGGAAC 
     2101 CACCTCCTAC TACCAAGAAA CCTGAACCTA CAACGCCGAA GGAGCCCGAA CCCACTACAC 
     2161 CGAAGGAGCC GGAACCGACT ACACCCAAGG AACCTGAGCC AACCACCCCG AAGGAACCCG 
     2221 AGCCTACGAC CCCTAAGGAG CCACCGCCCA CCACTAAAAA GCCCGAGCCC ACTACACCGA 
     2281 AAGAGCCTGA GCCGACGACC CCAAAAGAGC CAGAACCAAC CACCACCAAG GAGCCCGAGC 
     2341 CCACTACCAC AAAGGAGCCT GAATCTACCA CGAGGAAGGA GCCAGAGCCT ACCACCCCGA 
     2401 AAGAACCGGA ACCGACGACG CCTAAGGAAC CCGAGCCTAC CACTCCCAAG GAGCCTGAGC 
     2461 CTACCACTCC GAAGGAGCCC GAACCTACAA CGCTTAAGGA ACCTGAGCCT ACCACTCCCA 
     2521 AAGAGCCAGA GCCGACAACA CCTAAAGAGC CAGAGCCTAC TACTCCCAAA GAACCTGAAC 
     2581 CTACAACCCC CAAGGAACCC GAGCCCACCA CGCCAAAGGA ACCGGAGCCC ACCACACCTA 
     2641 AAGAGCCCGA ACCCACAACA CCAAAAGAAC CTGAGCCTAC AACGCCAAAG GAACCCGAGC 
     2701 CTACTACACC TAAGGAACCA GAGCCCACAA CCCCTAAAGA ACCTGAACCC ACTACCCCAA 
     2761 AAGAGCCTGA GCCAACTACA TTGAAAGAGC CAGAACCCAC AACACCCAAG GAACCCGAAC 
     2821 CTACCACACC CAAAGAGCCG GAGCCAACGA CCTTGAAGGA ACCAGAGCCC ACCACCCCAA 
     2881 AGGAGCCTGA GCCAACAACC CTGAAAGAGC CTGAGCCTAC GACCCCAAAG GAGCCCGAAC 
     2941 CTACAACAAC AAAGGAACCG GAAAGCACAA CACGAAAGGA ACCCGAGCCC ACAACTCCCA 
     3001 AGGAGCCTGA GCCCACCACC CCCAAAGAAC CTGAGCCTAC CACCCCTAAA GAGCCAGAGC 
     3061 CTACAACACC TAAGGAACCT GAACCAACGA CGCCAAAAGA ACCCGAACCA ACTACCCCCA 
     3121 AAGAACCTGA ACCCACCACC CCGAAAGAAC CAGAGCCCAC AACACCCAAA AAACCAGAGC 
     3181 CAACAACACC AAAGGAGCCT GAGCCCACCA CACCTAAAGA ACCCGAACCC ACTACTCCTA 
     3241 AAGAGCCCGA GCCAACAACA CCCAAGGAGC CTGAACCTAC GACTCGGAAG GAGCCCGAGC 
     3301 CCACTACTCC TAAAGAGCCC GAGCCTACCA CTCCAAAAGA ACCCGAGCCA ACGACACCCA 
     3361 AAAAACCCGA GCCCACAACT ACCTCCCCCA AAACTACTAC CTTGAAGGCT ACTACTCTGG 
     3421 CACCAAAGGT GACTGCCCCT GCCGAAGAGA TCCAAAACAA GCCTGAGGAA ACAACGCCTG 
     3481 CTAGTGAAGA CAGCGACGAC TCAAAGACTA CTCTCAAACC TCAGAAGCCT ACTAAAGCAC 
     3541 CCAAGCCTAC GAAGAAGCCT ACCAAGGCGC CAAAAAAACC GACAAGCACA AAAAAACCTA 
     3601 AAACACCCAA GACCCGCAAG CCTAAGACTA CTCCATCTCC ATTGAAGACC ACGAGCGCCA 
     3661 CTCCAGAGCT GAACACTACT CCACTGGAGG TCATGCTTCC AACCACAACC ATCCCCAAAC 
     3721 AAACACCAAA TCCGGAGACA GCTGAGGTGA ATCCTGATCA TGAAGACGCC GACGGGGGCG 
     3781 AGGGCGAAAA ACCGCTTATT CCTGGCCCCC CTGTACTTTT CCCCACTGCC ATTCCCGGTA 
     3841 CCGATTTGCT GGCTGGGCGA CTTAATCAAG GGATCAACAT AAACCCGATG CTGAGTGACG 
     3901 AGACAAACCT TTGTAATGGC AAGCCTGTTG ACGGCCTGAC CACACTCAGG AACGGCACAT 
     3961 TGGTGGCGTT TCGGGGCCAC TACTTTTGGA TGCTGAACCC TTTCCGCCCC CCCTCTCCAC 
     4021 CACGCAGGAT TACAGAAGTT TGGGGAATAC CAAGCCCAAT CGATACTGTG TTCACAAGGT 
     4081 GTAACTGTGA GGGGAAGACA TTCTTTTTCA AAGACTCCCA GTACTGGAGG TTCACCAACG 
     4141 ACGTAGTGGA TCCAGGCTAC CCGAAGCAGA TCGTGAAAGG TTTTGGGGGC CTGACGGGAA 
     4201 AGATCGTAGC GGCCCTTTCC ATCGCCAAAT ACAAAGATCG ACCAGAATCT GTGTATTTTT 
     4261 TCAAGAGAGG AGGTAATATC CAACAATATA CTTATAAGCA GGAACCCATG AAGAAGTGTA 
     4321 CTGGCAGGCG CCCAGCCATA AACTATAGCG TGTACGGGGA GGCAGCCCAG GTCAGACGGC 
     4381 GGCGCTTCGA AAGGGCAGTC GGACCCTTCC AGACGCACAC TTTTCGCATT CACTACTCCG 
     4441 TGCCAATGAG AGTCTCTTAC CAGGATAAGG GGTTTTTGCA CAACGAGGTG AAAGTTAGCA 
     4501 CTATGTGGCG CGGATTCCCA AACGTGGTGA CCAGTGCAAT CACACTGCCC AATATCCGAA 
     4561 AGCCAGACGG CTACGACTAT TATGCCTTCA GCAAGGACCA GTACTATAAC ATCGACGTTC 
     4621 CAACCAGAAC TGCCCGGGCC ATTACCACAC GAAGCGGCCA GACACTGTCA AAGATTTGGT 
     4681 ATAATTGTCC CACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4741 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4801 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4861 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4921 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4981 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5041 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5101 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5161 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5221 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5281 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5341 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5401 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5461 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5521 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5581 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5641 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5701 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5761 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5821 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5881 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5941 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6001 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6061 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6121 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6181 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6241 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6301 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6361 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6421 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6481 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6541 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6601 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6661 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6721 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6781 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6841 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6901 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6961 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7021 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7081 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7141 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7201 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7261 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7321 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7381 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7441 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7501 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7561 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7621 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7681 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7741 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7801 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7861 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7921 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7981 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8041 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8101 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8161 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8221 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8281 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8341 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8401 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8461 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8521 TAACAAAAAT TTAACGCGAA TTTT
//