LOCUS       MR222502                8283 bp    DNA     circular  SYN 
DEFINITION  Tie1 (Myc-DDK-tagged) - Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie1)
ACCESSION   NM_011587
VERSION     NM_011587.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4440..4531 
                     /label="Myc-DDK"  
     misc_feature    4561..5146 
                     /label="PolyA signal"  
     misc_feature    4618..4637 
                     /label="Restriction Enzyme"  
     misc_feature    5422..6094 
                     /label="ColE1"  
     misc_feature    6524..7318 
                     /label="Kan/Neo"  
     misc_feature    7353..7655 
                     /label="SV40 ori"  
     misc_feature    7741..8218 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4422..4428 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4422 
                     /label="Tie1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCTGGTGG GGATCCTCTT TGCTGCTCCC CACTCTTTTC TTGGCCTCTC 
     1081 ATGTTGGTGC GTCTGTTGAC TTAACATTGC TGGCCAACCT GCGCATCACA GACCCCCAGC 
     1141 GTTTCTTCCT GACCTGCGTG TCTGGTGAGG CCGGAGCAGG GAGGAGCTCG GACCCGCCCC 
     1201 TGCTGCTGGA GAAGGATGAT CGCATCGTGC GCACCTTCCC GCCTGGGCAG CCCCTGTACC 
     1261 TGGCACGCAA CGGTTCTCAC CAGGTCACGC TGAGGGGCTT CTCCAAGCCC TCGGATTTGG 
     1321 TAGGCGTCTT CTCCTGTGTG GGTGGTGCTG GCGCGCGGCG CACTCGAGTT CTCTATGTGC 
     1381 ACAACAGCCC AGGGGCACAC CTGTTTCCAG ACAAGGTCAC ACACACGGTG AACAAAGGTG 
     1441 ACACTGCTGT GCTTTCTGCC CATGTGCACA AGGAAAAGCA GACTGACGTG ATCTGGAAGA 
     1501 ACAACGGATC CTACTTCAAT ACCCTAGACT GGCAAGAGGC CGATGACGGG CGTTTTCAAC 
     1561 TGCAGCTCCA AAATGTACAG CCACCATCAA GTGGCATCTA CAGTGCCACC TACCTAGAGG 
     1621 CCAGCCCCCT GGGCAGTGCC TTCTTTCGGC TCATCGTGCG AGGCTGTGGG GCTGGACGCT 
     1681 GGGGGCCAGG ATGTGTCAAG GATTGCCCAG GCTGCCTGCA TGGAGGTGTC TGTCATGACC 
     1741 ATGATGGCGA ATGTGTGTGT CCCCCAGGAT TCACTGGCAC CCGCTGTGAG CAGGCCTGCA 
     1801 GGGAGGGTCG TTTTGGACAG AGCTGTCAGG AACAGTGCCC AGGCACAGCA GGTTGTAGAG 
     1861 GTCTCACCTT CTGCCTCCCG GATCCCTATG GCTGTTCCTG TGGATCTGGC TGGAGAGGAA 
     1921 GCCAGTGCCA GGAAGCATGT GCACCTGGTC ATTTTGGGGC TGATTGTCGC CTCCAGTGCC 
     1981 AGTGTCAAAA TGGTGGCACT TGTGACCGGT TCAGCGGCTG CGTCTGCCCC TCTGGGTGGC 
     2041 ATGGAGTCCA CTGTGAAAAG TCAGACCGGA TCCCCCAGAT CCTCAGTATG GCCACAGAGG 
     2101 TGGAGTTCAA CATAGGGACG ATGCCCCGGA TCAACTGTGC AGCTGCCGGG AATCCCTTCC 
     2161 CAGTGCGGGG CAGCATGAAA CTTCGCAAGC CAGATGGCAC CATGCTTTTG TCTACCAAAG 
     2221 TCATTGTGGA GCCAGACAGG ACCACAGCAG AGTTTGAAGT GCCCAGTTTG ACTCTTGGGG 
     2281 ACAGTGGGTT CTGGGAATGC CGCGTATCGA CTTCTGGTGG CCAAGACAGC CGGCGCTTCA 
     2341 AGGTCAATGT CAAAGTACCC CCAGTGCCTT TGACTGCACC TCGACTCCTG GCCAAGCAGA 
     2401 GCCGTCAGCT TGTGGTCTCC CCACTGGTCT CCTTTAGTGG GGATGGACCC ATCTCCTCTG 
     2461 TCCGCCTGCA CTACCGGCCC CAGGACAGCA CGATTGCCTG GTCTGCCATT GTGGTGGATC 
     2521 CCAGTGAGAA TGTGACATTA ATGAACCTGA AGCCAAAGAC AGGATACAAT GTCCGAGTGC 
     2581 AGCTGAGCCG GCCAGGGGAA GGAGGAGAAG GAGGCTGGGG ACCTTCTGCC CTTATGACTA 
     2641 CTGACTGTCC TGAGCCTTTG CTGCAGCCCT GGCTAGAGAG CTGGCACGTG GAGGGTCCTG 
     2701 ACAGGCTCCG TGTGAGCTGG TCTCTACCCT CGGTGCCACT GTCCGGTGAT GGTTTCCTGC 
     2761 TGCGCCTGTG GGATGGGGCC CGGGGACAGG AGAGGCGGGA GAACATCTCA TTCCCCCAGG 
     2821 CCCGCACTGC CCTCCTGACT GGACTCACGC CGGGCACCCA CTACCAGCTG GATGTGCGAC 
     2881 TGTATCACTG TACCCTCCTG GGCCCTGCCT CACCCCCTGC GCATGTGCAC CTGCCCCCCA 
     2941 GCGGGCCTCC AGCTCCCCGC CACCTCCATG CCCAGGCCCT CTCAGACTCT GAGATTCAAC 
     3001 TCATGTGGCA GCATCCCGAG GCTCCGTCTG GGCCTATATC CAAGTACATC GTGGAGATTC 
     3061 AGGTGGCCGG GGGCTCAGGA GACCCACAGT GGATGGATGT AGACAGGCCC GAGGAGACAA 
     3121 GCATCATTGT TCGTGGCCTC AATGCTAGCA CACGATATCT TTTCCGTGTG CGGGCCAGTG 
     3181 TGCAGGGTCT TGGCGACTGG AGCAATACAG TAGAAGAGGC CACTCTGGGC AATGGGCTGC 
     3241 AGAGTGAGGG CCCAGTCCGA GAAAGCCGGG CAGCTGAAGA AGGCCTGGAT CAGCAGCTGG 
     3301 TCCTGGCTGT GGTAGGTTCC GTCTCTGCCA CCTGCCTCAC CATCCTGGCT GCCCTTTTAG 
     3361 CCTTGGTGTG TATCCGAAGA AGCTGCCTAC ATCGGAGACG CACCTTCACT TACCAGTCAG 
     3421 GATCGGGTGA AGAGACCATC CTGCAGTTCA GCTCAGGGAC CTTGACCTTG ACCCGGCGAC 
     3481 CGAAGCCACA GCCTGAGCCC TTGAGTTACC CCGTGCTGGA GTGGGAGGAC ATCACCTTTG 
     3541 AGGACCTCAT AGGGGAGGGC AACTTTGGCC AAGTGATCCG GGCCATGATC AAGAAGGACG 
     3601 GGCTCAAGAT GAACGCAGCC ATCAAGATGC TAAAAGAGTA TGCGTCTGAA AATGACCATC 
     3661 GAGACTTTGC AGGTGAACTA GAAGTTCTGT GCAAACTAGG ACACCACCCC AATATTATCA 
     3721 ACCTCTTGGG GGCCTGTGAG AACCGAGGCT ACTTGTATAT TGCTATCGAA TACGCCCCCT 
     3781 ATGGAAACCT ACTGGACTTC CTGAGAAAGA GCAGGGTCCT GGAGACTGAT CCAGCTTTTG 
     3841 CTCGAGAGCA TGGGACGGCC TCCACACTCA GCTCTCGGCA GCTGCTGCGC TTTGCCAGTG 
     3901 ATGCAGCCAA CGGCATGCAG TACCTTAGTG AGAAGCAGTT CATTCACAGG GACCTGGCTG 
     3961 CCCGAAATGT GCTGGTCGGA GAGAACCTGG CCTCCAAGAT TGCAGACTTT GGCCTTTCTC 
     4021 GAGGGGAGGA GGTGTATGTG AAGAAGACAA TGGGCCGTCT CCCTGTGCGT TGGATGGCCA 
     4081 TTGAGTCCCT CAACTACAGC GTCTATACCA CCAAGAGTGA TGTCTGGTCC TTTGGGGTCC 
     4141 TCCTCTGGGA GATAGTGAGC CTTGGAGGCA CGCCCTACTG TGGCATGACC TGTGCTGAGC 
     4201 TCTACGAGAA GCTGCCTCAA GGCTATCGCA TGGAGCAGCC TCGAAACTGC GATGACGAAG 
     4261 TGTACGAGCT GATGCGGCAG TGCTGGCGGG ATCGGCCCTA TGAGCGCCCC CCCTTTGCTC 
     4321 AGATCGCACT ACAGTTGGGC CGCATGCTGG AAGCCAGGAA GGCCTACGTG AACATGTCGC 
     4381 TGTTTGAGAA CTTCACCTAT GCGGGCATCG ATGCCACAGC TGAGGAGGCC ACGCGTACGC 
     4441 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4501 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4561 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4621 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4681 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4741 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4801 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4861 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4921 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4981 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5041 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5101 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5161 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5221 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5281 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5341 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5401 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5461 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5521 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5581 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5641 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5701 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5761 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5821 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5881 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5941 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6001 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6061 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6121 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6181 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6241 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6301 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6361 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6421 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6481 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6541 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6601 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6661 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6721 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6781 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6841 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6901 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6961 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7021 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7081 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7141 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7201 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7261 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7321 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7381 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7441 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7501 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7681 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7741 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7801 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7861 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7921 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7981 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8041 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8101 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8161 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8221 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8281 TTT
//