LOCUS       MR222401                7995 bp    DNA     circular  SYN 
DEFINITION  Kif17 (Myc-DDK-tagged) - Mouse kinesin family member 17 (Kif17), transcript variant 1
ACCESSION   NM_010623
VERSION     NM_010623.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4152..4243 
                     /label="Myc-DDK"  
     misc_feature    4273..4858 
                     /label="PolyA signal"  
     misc_feature    4330..4349 
                     /label="Restriction Enzyme"  
     misc_feature    5134..5806 
                     /label="ColE1"  
     misc_feature    6236..7030 
                     /label="Kan/Neo"  
     misc_feature    7065..7367 
                     /label="SV40 ori"  
     misc_feature    7453..7930 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4134..4140 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4134 
                     /label="Kif17"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCGGAG TCAGTGAAAG TGGTCGTGCG CTGCCGACCC ATGAACAAGC 
     1081 GCGAGCGCGA GCTGAGCTGC CAGTCGGTGG TGACGGTGGA CAGTGCGCGC GGCCAATGCT 
     1141 TCATTCAGAA CCCGGGAGCG GCGGACGAAC CCCCAAAGCA GTTCACCTTC GATGGCGCCT 
     1201 ACTACATAGA ACACTTCACC GAGCAGATCT ACAACGAGAT CGCCTACCCG CTGGTGGAGG 
     1261 GTGTCACCGA AGGCTACAAT GGGACCATTT TTGCCTATGG CCAGACAGGC AGTGGGAAGT 
     1321 CTTTCACCAT GCAGGGACTG CCAGATCCCC CCTGCCAGAG GGGCATCATT CCCAGAGCCT 
     1381 TCGAGCACGT GTTTGAGAGC GTTCAGTGTG CAGAGAACAC CAAGTTCTTA GTGCGGGCTT 
     1441 CCTATCTGGA GATCTACAAC GAAGATGTCC ATGACCTGCT GGGCGCTGAC ACCAAGCAGA 
     1501 GGCTTGAGCT GAAGGAGCAT CCCGAGAAGG GAGTGTATGT CAAGGGGCTG TCCATGCACA 
     1561 CGGTACACAA CGTGGCCCAG TGCGAGCGCG TCATGGAGAC CGGCTGGAAG AACCGTGCGG 
     1621 TTGGCTACAC CCTGATGAAT AAGGACTCCT CCCGTTCCCA CTCCATCTTT ACCATCAACA 
     1681 TCGAGATCTA TGCCGTGGAT GAACGGGGTA AGGACCATCT TCGTGCAGGC AAGCTCAACC 
     1741 TGGTAGACCT GGCCGGCAGT GAGCGGCAAT CCAAGACTGG GGCCACAGGG GAAAGGCTGA 
     1801 AGGAGGCCAC CAAAATTAAC CTGTCACTGT CGGCGCTGGG CAACGTCATC TCGGCACTGG 
     1861 TGGATGGGCG CTGCAAGCAC ATTCCTTACC GGGACTCGAA GCTGACCCGA CTGCTTCAGG 
     1921 ACTCACTGGG TGGCAACACC AAGACTCTGA TGGTGGCTTG CCTGTCTCCC GCAGACAACA 
     1981 ATTACGATGA GACCCTCAGT ACGCTGCGCT ATGCTAACAG GGCCAAGAAC ATCAAGAACA 
     2041 AGCCACGCAT TAATGAAGAC CCCAAGGATG CCCTGCTTCG AGAGTACCAG GAAGAGATCA 
     2101 AGAGGCTAAA GGCTATCCTC GCCCAGCAGA TGGGCCCTGG CAACCTGTCA GCCCTGCTGT 
     2161 CCACTCAGAC ACCCCCAGGC CCAGTCCAGT CTGAGGAGAA GCTGCTGTCC CCAACTACTG 
     2221 TCCAGCAGGA CACGGAGGCT GAGAAGCAGT TGATCCGAGA GGAGTATGAG GAGCGCCTGG 
     2281 CCCGCCTGAA GGCTGACTAC GAGGCAGAGC AAGAGTCTCG GGTCCGGCTG CAGGAGGACA 
     2341 TTACTGCCAT GCGTAACTCG TATGACGTGA AGCTGTCCAC ACTGCAGGAG AATCTGCGCA 
     2401 AGGAGAAGGA GACAGAGGCC ATTCTCAAGG CGGAAGTCCT GTGTAAGACG GAGGTCATGT 
     2461 CCAGGGCCGA GTTAGCCAGC GGGCCCGAGT ACTCACCTCC TTTGCAGTAT GAGACTGCGG 
     2521 TGAAGCCCAC CATCCTCTCC ATGCCCGACA TGCCACCCAG TGGCAAGGTC ACCAAGAGCC 
     2581 AAGCGCCCCT CGCTTTCGAG GAACCACACG GGGAGACCTC CAGGTCCGAG TTTTCCTTTG 
     2641 AGTCCAATGA GTGTTCCACT CTCGAAGACT CTGCCACATC AGAGGCCTTC CCTGGGCCAG 
     2701 AGGAGTTTTC CAACATGGAG TTCTCCATGG CTGCGGCACT GACCGAGAGC AGGTACCTTC 
     2761 CTGAGGAGTA TCTCGGCGGC CAGGAGGCTG CTGCATCTCC GCTGGAGGCA GAGCGCTATG 
     2821 TCCAGGAGAA CGAGCCATCG CTGGAGCCCC TGCGGATACT GGCAAGCCTG CAGGATCCTT 
     2881 TTGCTGAAGT GGAGGCCAAG CTGGCCAGAC TGTCCTCTAC TGTGGCCATG TCGGACTCAT 
     2941 CCCAGACCGT CGTCCCCCAG ATCCCTAAGC AGCCATCCTC GGCTGACCTG CTGGAGCCCA 
     3001 GCGACACCAA GTCAGAAGCT GATGTGGCTG TGGCTGACAA CGTCGTCCTC GGGACTGAGC 
     3061 CTGATGTAAA CCTGAGAGTG GCTGAGGAGG TGGTTTCTGA GGCAGAGACT GGCGTGTGGA 
     3121 TGGAGTCTGA AGCCCAGGTG GCCCATGTGG CCCAGGTGTC TGAGGAGGCC CAGCCTCAGC 
     3181 CCTTGCTGGC CATGGTGAGC GTGAGGAGGG AAAGTGTGGG TGTAGAAGTG GCTGTGTTGA 
     3241 CGGAGGAGGA GCTGCAGCCT GTGGATCAAC AGCAGGTGCT GGCCCGCTTG CAGTTGCTGG 
     3301 AGCAGCAGGT GGTGGGGGGC GAGCAGGCTA AAAACAAGGA CCTGAGGGAG AAGCACAAGC 
     3361 GGCGGAAGCG GTACGCAGAT GAGCGCAAGA AGCAGCTGGT GGCCGCACTG CAGAACTCGG 
     3421 ATGAGGATGG CGGGGACTGG GTGCTGCTCA ACGTCTATGA CTCTATCCAG GAGGAAGTGA 
     3481 GGGCCAAGAG CAAGCTGCTG GAGAAGATGC AGAGGAAGCT CCGGGCAGCA GAGGTAGAAA 
     3541 TCAAGGACCT GCAGTCAGAG TTCCAGCTGG AGAAGATTGA TTACCTGGCA ACCATCCGCC 
     3601 GGCAGGAGCG GGACTCCATG CTCTTCCAGC AACTCCTGGA GCAGGTGCAG CCTCTCATCC 
     3661 GCAGAGACTG TAACTATAGC AACCTGGAGA AGATCAGGCG AGAGTCGAGC TGGGATGAGG 
     3721 ATAATGGCTT CTGGAAGATC CCAGATCCCA TCATACTGAA AACCAGCCTC CCAGTAGTTC 
     3781 CAACTGGGAC ACAGAACAAG CCAGCTCGAA AAACCTCTGC AGTAGACAGT GGGGAGCCAC 
     3841 ACATGCAGGA GGAAGACAGG TATAAGCTGA TGCTCAGCCG AAGCGACAGT GAGAATATCG 
     3901 CTAGCAACTA CTTCCGCTCC AAGCGGGCCA GCCAGATCCT CAGCACGGAC CCCATGAAGA 
     3961 GCCTCACATA TCACAACTCA CCACCGGGGC TAAATTCCTC TCTGAGCAAC AACTCTGCCT 
     4021 TGCCGCCCAC CCAGACCCCG GAAATGCCCC AGCCCCGGCC CTTCCGCCTA GAGTCCCTTG 
     4081 ACATCCCCTT CTCCAAAGCC AAGCGTAAAA AAAGCAAAAA CAGCTTCGGT GGTGAGCCTC 
     4141 TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4201 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4261 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4321 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4381 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4441 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4501 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4561 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4621 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4681 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4741 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4801 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4861 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4921 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4981 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5041 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5101 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5161 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5221 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5281 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5341 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5401 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5461 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5521 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5581 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5641 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5701 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5761 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5821 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5881 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5941 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6001 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6061 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6121 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6181 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6241 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6301 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6361 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6421 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6481 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6541 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6601 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6661 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6721 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6781 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6841 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6901 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6961 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7021 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7081 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7141 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7201 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7261 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7321 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7381 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7441 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7501 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7561 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7621 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7681 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7741 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7801 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7861 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7921 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7981 TTTAACGCGA ATTTT
//