LOCUS       MR222243L1                9036 bp    DNA     circular  SYN 
DEFINITION  Nedd4 (Myc-DDK-tagged) - Mouse neural precursor cell expressed, developmentally down-regulated 4 (Nedd4)
ACCESSION   NM_010890
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7327..7507 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8235..8894 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7666..8234 
                     /label="ColE1"  
     misc_feature    6202..6222 
                     /label="LR50 primer"  
     misc_feature    5999..6070 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6211..6802 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5981..5987 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5981 
                     /label="Nedd4"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAGCTCGGAC ATGGCAGCCG ACGAGTCGGA 
     3361 GGCCCCAGTA CTCTCGGAGG ACGAGGTATG GGAGTTTTGC CTGGATAAGA CAGAAGATGG 
     3421 TGGCGGATCC CCCGGAAGTG ATGTTACAGA CACTTGTGAG CCTCCATGTG GATGCTGGGA 
     3481 GTTGAATCCG AATTCCCTGG AAGAGGAGCA CGTGCTGTTC ACTGCTGATC CGTACCTGGA 
     3541 GCTCCACAAC GATGACACAC GAGTTGTGAG AGTGAAGGTT ATAGCTGGCA TAGGCCTGGC 
     3601 CAAGAAAGAC ATCTTGGGAG CCAGTGATCC TTACGTAAGA GTGACATTGT ATGACCCGAT 
     3661 GAGTGGAATC CTTACCAGCG TGCAGACAAA AACTATCAAA AAGTCTTTGA ATCCAAAATG 
     3721 GAATGAAGAA ATACTGTTCA GGGTCCTTCC ACAGCGACAC CGCATTCTTT TCGAAGTGTT 
     3781 TGATGAAAAT CGTTTGACAA GAGATGATTT CCTAGGTCAA GTGGATGTCC CTCTCTATCC 
     3841 TTTACCGACT GAAAACCCAA GAATGGAGAG ACCATATACA TTTAAGGATT TTGTTCTTCA 
     3901 TCCAAGAAGT CACAAATCAA GAGTTAAAGG TTATCTGAGA TTAAAAATGA CTTATTTACC 
     3961 TAAAAATGGC TCAGAAGATG AAAATGCAGA CCAGGCTGAG GAGTTAGAGC CTGGCTGGGT 
     4021 TGTTTTGGAC CAACCAGATG CTGCCACTCA TTTGCCGCAT CCACCAGAAC CCTCTCCCCT 
     4081 ACCTCCAGGA TGGGAAGAGA GGCAGGATGT CCTTGGAAGG ACCTACTACG TAAACCATGA 
     4141 ATCTAGAAGA ACACAGTGGA AAAGGCCAAG CCCTGACGAT GACCTCACGG ATGAAGACAA 
     4201 TGATGATATG CAGCTGCAAG CGCAGCGAGC ATTCACCACC AGGCGGCAGA TATCGGAGGA 
     4261 TGTGGATGGC CCTGACAACC GGGAGTCCCC TGAGAATTGG GAAATCGTAC GAGAAGATGA 
     4321 AAACACCGAG TATAGTGGTC AGGCTGTCCA GTCACCTCCA TCGGGTCACA TTGATGTGCA 
     4381 GACTCACCTT GCAGAAGAGT TTAATACCAG ACTTGCCGTG TGTGGAAATC CAGCCACCAG 
     4441 CCAGCCGGTT ACCAGCTCAA ATCATTCCAG CAGAGGAGGC AGCTTGCAGA CCTGTATCTT 
     4501 TGAGGAACAG CCTACACTTC CTGTGCTTTT GCCTACTTCA TCTGGATTGC CACCAGGTTG 
     4561 GGAAGAAAAA CAAGATGACA GAGGAAGATC ATACTATGTA GACCACAACT CTAAAACCAC 
     4621 CACATGGTCC AAGCCCACCA TGCAGGATGA TCCAAGATCG AAAATCCCTG CTCATCTGAG 
     4681 AGGAAAGACT GACTCCAATG ACCTGGGACC CTTACCTCCA GGCTGGGAAG AAAGAACCCA 
     4741 CACAGATGGG CGAGTCTTCT TCATAAACCA CAATATAAAG AAGACCCAGT GGGAAGATCC 
     4801 TCGCCTGCAG AACGTGGCAA TCACTGGACC AGCAGTGCCC TACTCCAGAG ATTACAAGAG 
     4861 AAAGTACGAG TTCTTCAGAA GGAAGCTCAA GAAGCAGACT GACATTCCAA ACAAATTTGA 
     4921 AATGAAGCTT CGCCGCGCAA ACATTCTGGA GGATTCTTAC CGGAGGATTA TGGGTGTGAA 
     4981 GAGAGCTGAC TTGCTCAAGG CCAGACTCTG GATTGAGTTT GATGGTGAAA AGGGCCTTGA 
     5041 CTATGGAGGG GTTGCCAGAG AGTGGTTCTT CCTCATCTCG AAGGAAATGT TCAACCCTTA 
     5101 CTACGGCCTG TTTGAATATT CTGCTACGGA TAATTACACC CTACAGATAA ATCCTAACTC 
     5161 GGGCTTGTGT AATGAAGATC ACCTCTCATA CTTCAAGTTC ATTGGCCGTG TGGCTGGGAT 
     5221 GGCAGTTTAT CATGGCAAGC TGTTGGATGG GTTTTTCATC CGTCCGTTTT ACAAGATGAT 
     5281 GCTTCAGAAA CTGATAACAC TGCACGACAT GGAGTCCGTG GATAGTGAAT ATTACAGTTC 
     5341 TCTGCGATGG ATTCTTGAAA ATGACCCGAC GGAGCTGGAC CTGAGATTTA TCATAGATGA 
     5401 AGAACTTTTT GGACAGACAC ATCAGCACGA ACTGAAAACC GGAGGATCAG AGATTGTTGT 
     5461 CACCAATAAG AACAAAAAGG AGTATATCTA CCTTGTAATA CAATGGCGAT TTGTGAACCG 
     5521 TATCCAGAAG CAAATGGCAG CTTTTAAAGA GGGATTTTTT GAACTGATAC CACAGGATCT 
     5581 CATCAAGATA TTTGATGAAA ATGAGCTAGA GCTTCTCATG TGTGGTCTGG GAGATGTGGA 
     5641 TGTGAACGAC TGGCGGGAAC ACACAAAATA CAAAAATGGC TACAGCATGA ACCACCAGGT 
     5701 CATCCACTGG TTCTGGAAGG CTGTTTGGAT GATGGATTCG GAAAAAAGAA TACGCTTACT 
     5761 TCAGTTTGTC ACTGGCACAT CCCGTGTGCC GATGAATGGG TTTGCTGAAC TCTATGGCTC 
     5821 GAATGGACCA CAATCCTTCA CAGTGGAACA ATGGGGCACC CCTGATAAGC TGCCAAGAGC 
     5881 ACACACCTGC TTCAATCGCC TGGACCTGCC ACCCTACGAA TCCTTTGACG AACTCTGGGA 
     5941 TAAACTTCAG ATGGCAATTG AGAACACACA GGGCTTTGAT GGCGTTGATA CGCGTACGCG 
     6001 GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA 
     6061 CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CTGTACAAGT AGGATTCGTC 
     6121 GAGGGACCTA ATAACTTCGT ATAGCATACA TTATACGAAG TTATACATGT TTAAGGGTTC 
     6181 CGGTTCCACT AGGTACAATT CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA 
     6241 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC 
     6301 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC 
     6361 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT 
     6421 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC 
     6481 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC 
     6541 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG 
     6601 GTGTTGTCGG GGAAATCATC GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT 
     6661 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC 
     6721 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT 
     6781 CGGATCTCCC TTTGGGCCGC CTCCCCGCAT CGATACCGTC GACCTCGATC GAGACCTAGA 
     6841 AAAACATGGA GCAATCACAA GTAGCAATAC AGCAGCTACC AATGCTGATT GTGCCTGGCT 
     6901 AGAAGCACAA GAGGAGGAGG AGGTGGGTTT TCCAGTCACA CCTCAGGTAC CTTTAAGACC 
     6961 AATGACTTAC AAGGCAGCTG TAGATCTTAG CCACTTTTTA AAAGAAAAGG GGGGACTGGA 
     7021 AGGGCTAATT CACTCCCAAC GAAGACAAGA TATCCTTGAT CTGTGGATCT ACCACACACA 
     7081 AGGCTACTTC CCTGATTGGC AGAACTACAC ACCAGGGCCA GGGATCAGAT ATCCACTGAC 
     7141 CTTTGGATGG TGCTACAAGC TAGTACCAGT TGAGCAAGAG AAGGTAGAAG AAGCCAATGA 
     7201 AGGAGAGAAC ACCCGCTTGT TACACCCTGT GAGCCTGCAT GGGATGGATG ACCCGGAGAG 
     7261 AGAAGTATTA GAGTGGAGGT TTGACAGCCG CCTAGCATTT CATCACATGG CCCGAGAGCT 
     7321 GCATCCGGAC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG 
     7381 CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT 
     7441 GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT 
     7501 GTGGAAAATC TCTAGCAGCA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     7561 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     7621 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     7681 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     7741 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     7801 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     7861 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     7921 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     7981 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     8041 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     8101 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     8161 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     8221 ACGTTAAGGG ATTTTGGTCA TGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT 
     8281 AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAAA CGGCATGATG AACCTGAATC 
     8341 GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG 
     8401 GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA 
     8461 TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA 
     8521 CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT 
     8581 TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA 
     8641 ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA TACGGAACTC CGGATGAGCA 
     8701 TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT 
     8761 ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA 
     8821 ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA 
     8881 TATCCAGTGA TTTTTTTCTC CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8941 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     9001 GTCCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGAC
//