LOCUS       MR222223                8448 bp    DNA     circular  SYN 
DEFINITION  Ptpn14 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase, non-receptor type 14 (Ptpn14)
ACCESSION   NM_008976
VERSION     NM_008976.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4605..4696 
                     /label="Myc-DDK"  
     misc_feature    4726..5311 
                     /label="PolyA signal"  
     misc_feature    4783..4802 
                     /label="Restriction Enzyme"  
     misc_feature    5587..6259 
                     /label="ColE1"  
     misc_feature    6689..7483 
                     /label="Kan/Neo"  
     misc_feature    7518..7820 
                     /label="SV40 ori"  
     misc_feature    7906..8383 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4587..4593 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4587 
                     /label="Ptpn14"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTTTCGGC CTGAAGCTCC GCAGGACTCG GCGCTACAAC GTCCTGAGCA 
     1081 AGAACTGCTT TGTTACCCGG ATCCGCCTGC TGGACAGCAA TGTCATCGAG TGCACGCTGT 
     1141 CGGTGGAAAG CACGGGGCAA GAGTGCCTGG AGGCCGTGGC CCAGAGGCTG GAGCTGAGGG 
     1201 AGACGCACTA CTTCGGCCTT TGGTTTCTCA GCAAGAGCCA GCAGGCGAGA TGGGTAGAGC 
     1261 TGGAGAAGCC ACTGAAGAAA CATCTGGACA AGTTTGCTAA CGAGCCTCTG CTTTTCTTCG 
     1321 GAGTCATGTT CTATGTGCCA AATGTGTCAC GGCTTCAGCA GGAGGCCACA AGATATCAGT 
     1381 ATTACCTGCA AGTCAAAAAA GACGTGCTTG AAGGACGGTT GCGGTGCTCC CTGGAACAAG 
     1441 TGATCCGGCT GGCTGGCTTA GCTGTGCAAG CTGACTTCGG AGATTATAAC CAGTTTGATT 
     1501 CCCAAGAGTT CCTCCGAGAG TATGTGCTCT TTCCTATGGA TTTGGCCATG GAGGAGGCGG 
     1561 CTCTGGAGGA GCTAACCCAG AAGGTGGCCC AGGAACACAA AGCTCATAGC GGGATCCTGC 
     1621 CGGCTGAAGC TGAACTGATG TACATCAACG AGGTAGAGCG TTTGGATGGA TTTGGACAGG 
     1681 AGATCTTCCC CGTGAAGGAC AGTCATGGCA ACAGCGTGCA CCTCGGCATC TTCTTCATGG 
     1741 GGATTTTTGT GAGGAACAGG GTCGGGAGAC AGGCAGTGAT ATACAGGTGG AATGACATTG 
     1801 GGAGTGTTAC TCACAGCAAA GCAGCCATCC TGTTGGAGCT GATTGACAAG GAGGAGACCG 
     1861 CGCTCTTCCA TACAGATGAT ATTGAAAATG CCAAGTACAT TTCTCGGTTG TTTACCACTC 
     1921 GGCACAAATT TTACAAACAG AACAAGATCT GCACTGAACA GTCAAATTCT CCACCCCCAA 
     1981 TTAGACGCCA GCCCACCTGG AGCCGGTCCT CACTGCCAAG GCAGCAGCCG TATATCTTGC 
     2041 CTCCCATGCA TGTCCAGTGC AGTGAGCACT ACTCGGAGAC CCATACTTCC CAAGACAGCA 
     2101 TTTTCCCCGG GAACGAAGAA GCCTTGTACT GCCGTTCTCA CAACAGCCTG GACCTTAATT 
     2161 ACTTGAACGG CACCGTCACC AATGGCAGCG TGTGCAGCGT TCACAGCGTC AACTCCCTCA 
     2221 GCTGCTCTCA GAGCTTCATC CAGGCGTCTC CAGTGTCCTC CAACCTTAGC ATCCCTGGGA 
     2281 GTGACATCAT GAGGGCCGAT TACATCCCCA GCCACCGCCA CAGCACCATC ATCGTGCCGT 
     2341 CTTACAGGCC GACCCCAGAT TACGAGACGG TCATGAGGCA GATGAAGAGG GGTCTGATGC 
     2401 ACGCAGACAG CCAGAGCCGG TCTCTGCGTA ACCTCAATAT CATCAACACC CATGCCTATA 
     2461 ACCAGCCCGA GGAACTGGTG TACAGCCAGC CGGAGATGCG GGAGAGGCAT CCCTACACGG 
     2521 TCCCCTATGC ACACCAGGGG TGCTACGGTC ACAAACTTGT AAGTCCGTCT GACCAGATGA 
     2581 ACCCCCAAAA TTGTGCGATG CCTATCAAGC CAGGGGCCAG TTCCATCTCA CACACAGTGA 
     2641 GCACTCCAGA ACTAGCCAAC ATGCAGCTCC AAGGAGCACA ACACTATAGC ACAGCCCACA 
     2701 TGCTCAAGAA CTATCTATTC AGGCCGCCAC CCCCTTACCC TCGGCCCCGT CCTGCCACCA 
     2761 GCACCCCAGA CCTCGCCAGC CACCGCCACA AGTACGTCAG CGGCAGCAGC CCTGATCTGG 
     2821 TAACTCGGAA GGTGCAGCTC TCCGTAAAGA CCTTCCAGGA GGACAGCTCA CCTGTGGTCC 
     2881 ATCAGTCTCT GCAGGAGGTG AGCGAACCCC TTACAGCCAC CAAGCACCAT GGCGGCGGCG 
     2941 GTGGCACGGT GAATAAACGC CACAGCCTGG AGGTGATGAA CAGCATGGTG AGAGGCATGG 
     3001 AGGCCATGAC ACTGAAGTCA CTCAATATCC CCATGGCTCG CCGCAACACC CTTCGGGAGC 
     3061 AGGGCCCTTC CGAGGAGACG GGCGGCCACG AAGTGCACGG TCTCCCCCAG TATCACCACA 
     3121 AGAAGACATT CTCGGATGCC ACCATGCTGA TCCACAGCAG TGAGAGCGAG GAAGAGGAGG 
     3181 AGACCCTGGA GGCTGCACCT CAGGTTCCTG TGCTTCGAGA GAAAGTAGAA TACAGTGCCC 
     3241 AGCTGCAGGC TGCCCTGGCC CGCATCCCCA ACAGGCCCCC ACCTGAGTAC CCAGGGCCAA 
     3301 GAAAAAGTGT CAGTAATGGG GCACTGAGAC AGGACCAGGG AACCCCTCTT CCTGCCATGG 
     3361 CCAGGTGCAG GGTGCTGAGA CACGGACCAT CCAAGGCCCT CAGTGTCTCC CGGGCAGAGC 
     3421 AGCTGGCTGT CAACGGTGCC TCTCTGGGTC CCTCCATCTC TGAGCCTGAC CTAACCAGCG 
     3481 TGAAGGAGCG GGTCAAGAAA GAGCCTGTGA AGGAAAGGCC GGTGTCAGAG ATGTTCTCCC 
     3541 TGGAGGACAG CATTATAGAG AGAGAGATGA TGATCAGGAA TCTAGAGAAG CAGAAGATGA 
     3601 CGGGCCCGCA GGCACAGAAG AGACCGCTGA TGTTGGCAGC GCTGAATGGG CTCTCGGTGG 
     3661 CCCGAGTGTC GGGGCGGGAA GACGGTCACC ATGATGCCAC CCGAGTCCCC ATAGACGAGA 
     3721 GGCTCAGAGC CCTGAAGAAG AAGCTGGAAG ATGGAATGGT GTTCACAGAA TATGAGCAGA 
     3781 TTCCAAACAA AAAGGCCAAC GGCGTCTTCA GCACCGCCAC TCTGCCTGAG AATGCCGAGC 
     3841 GCAGCCGGAT CCGAGAAGTT GTCCCATACG AGGAGAATCG AGTGGAGCTC ATCCCGACCA 
     3901 AAGAAAACAA CACAGGCTAT ATCAACGCCT CCCACATCAA GGTGGTGGTC GGCGGATCAG 
     3961 AATGGCACTA CATCGCCACC CAGGGGCCCT TGCCACATAC GTGCCATGAC TTCTGGCAGA 
     4021 TGGTGTGGGA GCAGGGGGTG AATGTGATCG CCATGGTCAC TGCAGAGGAG GAGGGTGGAC 
     4081 GGACCAAAAG CCATCGATAC TGGCCCAAAC TGGGGTCCAA GCATAGTTCT GCCACCTACG 
     4141 GCAAGTTCAA GGTCACCACA AAGTTCCGGA CAGATTCTGG TTGCTATGCA ACGACGGGCC 
     4201 TAAAGGTGAA GCACCTGCTG TCCGGGCAGG AGAGGACCGT GTGGCACTTG CAGTACACGG 
     4261 ACTGGCCCCA CCACGGCTGT CCAGAAGACG TCCAAGGATT TTTGTCCTAC TTGGAGGAAA 
     4321 TCCAGTCAGT CCGACGCCAC ACCAACAGCG TGCTGGAAGG CATCAGGACC AGGCACCCCC 
     4381 CCATCGTGGT TCACTGCAGC GCGGGTGTGG GAAGGACTGG TGTGGTTATC CTCTCTGAGC 
     4441 TCATGATCTA CTGCCTGGAA CACAACGAAA AGGTGGAGGT GCCCACGATG CTGCGATTCC 
     4501 TCAGGGAGCA GAGGATGTTC ATGATCCAGA CCATTGCGCA GTACAAGTTC GTCTACCAAG 
     4561 TCCTCGTCCA GTTCCTGCAG AATTCCAGGC TCATTACGCG TACGCGGCCG CTCGAGCAGA 
     4621 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4681 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4741 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4801 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4861 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4921 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4981 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5041 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5101 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5161 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5221 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5281 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5341 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5401 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5461 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5521 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5581 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5641 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5701 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5761 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5821 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5881 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5941 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6001 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6061 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6121 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6181 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6241 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6301 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6361 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6421 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6481 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6541 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6601 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6661 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6721 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6781 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6841 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6901 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6961 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7021 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7081 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7141 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7201 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7261 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7321 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7381 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7441 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7501 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7561 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7621 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7681 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7801 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7861 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7921 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7981 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8041 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8101 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8161 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8221 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8281 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8341 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8401 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//